Restriction map for
pF1KE0469
(Circular) (Six-base) MAPSORT of: pF1KE0469.seq Check: 4592 from: 1 to: 3737
pF1KE0469 3737 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 582 582
Size: 3737
AccI GT'mk_AC
Cuts at: 775 775
Size: 3737
Acc65I G'GTAC_C
Cuts at: 753 753
Size: 3737
AclI AA'CG_TT
Cuts at: 3577 3577
Size: 3737
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 329 1562 1994 2741 3009 329
Size: 1233 432 747 268 1057
Fragments arranged by size:
1233 1057 747 432 268
AflIII A'CryG_T
Cuts at: 1089 2193 3170 1089
Size: 1104 977 1656
Fragments arranged by size:
1656 1104 977
AgeI A'CCGG_T
Cuts at: 2125 2125
Size: 3737
AhdI GACnn_n'nnGTC
Cuts at: 175 175
Size: 3737
AlwNI CAG_nnn'CTG
Cuts at: 597 1042 2609 597
Size: 445 1567 1725
Fragments arranged by size:
1725 1567 445
ApaI G_GGCC'C
Cuts at: 117 126 253 117
Size: 9 127 3601
Fragments arranged by size:
3601 127 9
ApaLI G'TGCA_C
Cuts at: 2507 2507
Size: 3737
ApoI r'AATT_y
Cuts at: 741 3090 3346 741
Size: 2349 256 1132
Fragments arranged by size:
2349 1132 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3737
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3737
AvaI C'yCGr_G
Cuts at: 757 757
Size: 3737
BamHI G'GATC_C
Cuts at: 762 762
Size: 3737
BanI G'GyrC_C
Cuts at: 153 753 1167 1415 1450 153
Size: 600 414 248 35 2440
Fragments arranged by size:
2440 600 414 248 35
BanII G_rGCy'C
Cuts at: 117 126 193 253 751 1781 117
Size: 9 67 60 498 1030 2073
Fragments arranged by size:
2073 1030 498 67 60 9
BbeI G_GCGC'C
Cuts at: 1171 1419 1171
Size: 248 3489
BbsI GAAGACnn'nnnn_
Cuts at: 317 346 317
Size: 29 3708
BciVI GTATCCnnnnn_n'
Cuts at: 1635 2396 3737 1635
Size: 761 1341 1635
Fragments arranged by size:
1635 1341 761
BclI T'GATC_A
Cuts at: 1257 1257
Size: 3737
BglI GCCn_nnn'nGGC
Cuts at: 112 121 112
Size: 9 3728
BglII A'GATC_T
Cuts at: 560 1252 3289 560
Size: 692 2037 1008
Fragments arranged by size:
2037 1008 692
BlpI GC'TnA_GC
Cuts at: 849 849
Size: 3737
Bme1580I G_kGCm'C
Cuts at: 117 126 158 253 1362 1455 2511 117
Size: 9 32 95 1109 93 1056 1343
Fragments arranged by size:
1343 1109 1056 95 93 32 9
BmgBI CAC'GTC
Cuts at: 402 402
Size: 3737
BmrI ACTGGGnnnn_n'
Cuts at: 169 418 949 1030 1365 3509 169
Size: 249 531 81 335 2144 397
Fragments arranged by size:
2144 531 397 335 249 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2104 2104
Size: 3737
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 302
Size: 3737
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 913 1792 2284 2582 913
Size: 879 492 298 2068
Fragments arranged by size:
2068 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3441 3441
Size: 3737
BsaAI yAC'GTr
Cuts at: 1090 1090
Size: 3737
BsaBI GATnn'nnATC
Cuts at: 6 1251 3288 6
Size: 1245 2037 455
Fragments arranged by size:
2037 1245 455
BsaHI Gr'CG_yC
Cuts at: 171 1168 1416 2118 3614 171
Size: 997 248 702 1496 294
Fragments arranged by size:
1496 997 702 294 248
BsaWI w'CCGG_w
Cuts at: 926 1447 2125 2399 2546 2957 926
Size: 521 678 274 147 411 1706
Fragments arranged by size:
1706 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 299 555 685 299
Size: 256 130 3351
Fragments arranged by size:
3351 256 130
BseYI C'CCAG_C
Cuts at: 577 1162 1920 2497 2993 577
Size: 585 758 577 496 1321
Fragments arranged by size:
1321 758 585 577 496
BsiEI CG_ry'CG
Cuts at: 104 1279 1325 2533 104
Size: 1175 46 1208 1308
Fragments arranged by size:
1308 1208 1175 46
BsiHKAI G_wGCw'C
Cuts at: 751 1529 1719 2511 751
Size: 778 190 792 1977
Fragments arranged by size:
1977 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 117 126 158 193 253 751 1362 1455 1529
Size: 9 32 35 60 498 611 93 74
Cuts at: 1529 1719 1781 2511 117
Size: 190 62 730 1343
Fragments arranged by size:
1343 730 611 498 190 93 74 62
60 35 32 9
BspEI T'CCGG_A
Cuts at: 2957 2957
Size: 3737
BspHI T'CATG_A
Cuts at: 2913 2913
Size: 3737
BspMI ACCTGCnnnn'nnnn_
Cuts at: 582 787 1001 1303 1684 582
Size: 205 214 302 381 2635
Fragments arranged by size:
2635 381 302 214 205
BsrBI CCG'CTC
Cuts at: 50 2029 2083 3567 3732 50
Size: 1979 54 1484 165 55
Fragments arranged by size:
1979 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1649 1649
Size: 3737
BsrFI r'CCGG_y
Cuts at: 203 1735 1916 2125 203
Size: 1532 181 209 1815
Fragments arranged by size:
1815 1532 209 181
BsrGI T'GTAC_A
Cuts at: 512 512
Size: 3737
BssSI C'ACGA_G
Cuts at: 2008 2366 2008
Size: 358 3379
BstAPI GCAn_nnn'nTGC
Cuts at: 1114 1114
Size: 3737
BstBI TT'CG_AA
Cuts at: 2098 2098
Size: 3737
BstYI r'GATC_y
Cuts at: 1 560 762 1229 1252 1587 1833 2845 2931
Size: 559 202 467 23 335 246 1012 86
Cuts at: 2931 2943 3289 1
Size: 12 346 449
Fragments arranged by size:
1012 559 467 449 346 335 246 202
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 417 417
Size: 3737
BtgI C'CryG_G
Cuts at: 107 722 107
Size: 615 3122
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 157 1687 1868 2031 157
Size: 1530 181 163 1863
Fragments arranged by size:
1863 1530 181 163
BtsI GCAGTG_nn'
Cuts at: 980 980
Size: 3737
DraI TTT'AAA
Cuts at: 736 736
Size: 3737
DrdI GACnn_nn'nnGTC
Cuts at: 1443 2301 1443
Size: 858 2879
EaeI y'GGCC_r
Cuts at: 205 555 612 1322 1496 1887 1914 3259 205
Size: 350 57 710 174 391 27 1345 683
Fragments arranged by size:
1345 710 683 391 350 174 57 27
EagI C'GGCC_G
Cuts at: 1322 1322
Size: 3737
EarI CTCTTCn'nnn_
Cuts at: 1760 1970 1760
Size: 210 3527
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1945 2255 2401 3547 1945
Size: 310 146 1146 2135
Fragments arranged by size:
2135 1146 310 146
EcoICRI GAG'CTC
Cuts at: 749 749
Size: 3737
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 329 1562 1994 2741 3009 302
Size: 27 1233 432 747 268 1030
Fragments arranged by size:
1233 1030 747 432 268 27
EcoNI CCTnn'n_nnAGG
Cuts at: 284 284
Size: 3737
EcoO109I rG'GnC_Cy
Cuts at: 113 122 249 250 876 113
Size: 9 127 1 626 2974
Fragments arranged by size:
2974 626 127 9 1
EcoRI G'AATT_C
Cuts at: 741 741
Size: 3737
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1756 1756
Size: 3737
FspI TGC'GCA
Cuts at: 1518 1518
Size: 3737
HaeII r_GCGC'y
Cuts at: 1171 1419 2441 3420 1171
Size: 248 1022 979 1488
Fragments arranged by size:
1488 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 161 193 1579 1609 1611 1641 161
Size: 32 1386 30 2 30 2257
Fragments arranged by size:
2257 1386 32 30 30 2
HincII GTy'rAC
Cuts at: 776 776
Size: 3737
HindIII A'AGCT_T
Cuts at: 488 547 488
Size: 59 3678
KasI G'GCGC_C
Cuts at: 1167 1415 1167
Size: 248 3489
KpnI G_GTAC'C
Cuts at: 757 757
Size: 3737
MluI A'CGCG_T
Cuts at: 3170 3170
Size: 3737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2408 2592 2408
Size: 184 3553
MscI TGG'CCA
Cuts at: 557 614 1498 557
Size: 57 884 2796
Fragments arranged by size:
2796 884 57
MslI CAynn'nnrTG
Cuts at: 1726 1853 1726
Size: 127 3610
MspA1I CmG'CkG
Cuts at: 848 1162 1522 2535 2780 848
Size: 314 360 1013 245 1805
Fragments arranged by size:
1805 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1918 1918
Size: 3737
NarI GG'CG_CC
Cuts at: 1168 1416 1168
Size: 248 3489
NcoI C'CATG_G
Cuts at: 107 107
Size: 3737
NgoMIV G'CCGG_C
Cuts at: 1916 1916
Size: 3737
NspI r_CATG'y
Cuts at: 790 1290 2197 790
Size: 500 907 2330
Fragments arranged by size:
2330 907 500
PasI CC'CwG_GG
Cuts at: 117 193 117
Size: 76 3661
PciI A'CATG_T
Cuts at: 2193 2193
Size: 3737
PmeI GTTT'AAAC
Cuts at: 736 736
Size: 3737
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2876 2908 2876
Size: 32 3705
PspOMI G'GGCC_C
Cuts at: 113 122 249 113
Size: 9 127 3601
Fragments arranged by size:
3601 127 9
PstI C_TGCA'G
Cuts at: 449 784 1469 449
Size: 335 685 2717
Fragments arranged by size:
2717 685 335
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3737
PvuII CAG'CTG
Cuts at: 1162 1522 1162
Size: 360 3377
RsrII CG'GwC_CG
Cuts at: 1932 1932
Size: 3737
SacI G_AGCT'C
Cuts at: 751 751
Size: 3737
SalI G'TCGA_C
Cuts at: 774 774
Size: 3737
SapI GCTCTTCn'nnn_
Cuts at: 1760 1970 1760
Size: 210 3527
SbfI CC_TGCA'GG
Cuts at: 784 784
Size: 3737
ScaI AGT'ACT
Cuts at: 3254 3254
Size: 3737
SfcI C'TryA_G
Cuts at: 33 347 445 780 1465 2458 2649 33
Size: 314 98 335 685 993 191 1121
Fragments arranged by size:
1121 993 685 335 314 191 98
SfiI GGCCn_nnn'nGGCC
Cuts at: 121 121
Size: 3737
SfoI GGC'GCC
Cuts at: 1169 1417 1169
Size: 248 3489
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3737
SmaI CCC'GGG
Cuts at: 759 759
Size: 3737
SmlI C'TyrA_G
Cuts at: 892 1807 2299 2561 892
Size: 915 492 262 2068
Fragments arranged by size:
2068 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3737
SphI G_CATG'C
Cuts at: 790 1290 790
Size: 500 3237
StyI C'CwwG_G
Cuts at: 107 627 871 1225 107
Size: 520 244 354 2619
Fragments arranged by size:
2619 520 354 244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1320 1453 1912 2121 1320
Size: 133 459 209 2936
Fragments arranged by size:
2936 459 209 133
TatI w'GTAC_w
Cuts at: 512 3252 512
Size: 2740 997
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3254 3286 3254
Size: 32 3705
Tth111I GACn'n_nGTC
Cuts at: 1534 1534
Size: 3737
XbaI T'CTAG_A
Cuts at: 768 768
Size: 3737
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 382 1232 382
Size: 850 2887
XmaI C'CCGG_G
Cuts at: 757 757
Size: 3737
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsaXI BsgI
BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PfoI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI