Restriction map for pF1KE0469
 (Linear) (Six-base) MAPSORT of: KIEE0469.seq  Check: 1530  from: 1  to: 624



KIEE0469.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     474     624 
   Size:        474     150
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     221     624 
   Size:        221     403
 
AhdI GACnn_n'nnGTC

Cuts at:      0      67     624 
   Size:         67     557
 
AlwNI CAG_nnn'CTG

Cuts at:      0     489     624 
   Size:        489     135
 
ApaI G_GGCC'C

Cuts at:      0       9      18     145     624 
   Size:          9       9     127     479

  Fragments arranged by size: 

                 479     127       9       9
 
BanI G'GyrC_C

Cuts at:      0      45     624 
   Size:         45     579
 
BanII G_rGCy'C

Cuts at:      0       9      18      85     145     624 
   Size:          9       9      67      60     479

  Fragments arranged by size: 

                 479      67      60       9       9
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     209     238     624 
   Size:        209      29     386

  Fragments arranged by size: 

                 386     209      29
 
BglI GCCn_nnn'nGGC

Cuts at:      0      13     624 
   Size:         13     611
 
BglII A'GATC_T

Cuts at:      0     452     624 
   Size:        452     172
 
Bme1580I G_kGCm'C

Cuts at:      0       9      18      50     145     624 
   Size:          9       9      32      95     479

  Fragments arranged by size: 

                 479      95      32       9       9
 
BmgBI CAC'GTC

Cuts at:      0     294     624 
   Size:        294     330
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      61     310     624 
   Size:         61     249     314

  Fragments arranged by size: 

                 314     249      61
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     194     624 
   Size:        194     430
 
BsaHI Gr'CG_yC

Cuts at:      0      63     624 
   Size:         63     561
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     191     447     577     624 
   Size:        191     256     130      47

  Fragments arranged by size: 

                 256     191     130      47
 
BseYI C'CCAG_C

Cuts at:      0     469     624 
   Size:        469     155
 
Bsp1286I G_dGCh'C

Cuts at:      0       9      18      50      85     145     624 
   Size:          9       9      32      35      60     479

  Fragments arranged by size: 

                 479      60      35      32       9       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     474     624 
   Size:        474     150
 
BsrFI r'CCGG_y

Cuts at:      0      95     624 
   Size:         95     529
 
BsrGI T'GTAC_A

Cuts at:      0     404     624 
   Size:        404     220
 
BstYI r'GATC_y

Cuts at:      0     452     624 
   Size:        452     172
 
Bsu36I CC'TnA_GG

Cuts at:      0     309     624 
   Size:        309     315
 
BtgI C'CryG_G

Cuts at:      0     614     624 
   Size:        614      10
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      49     624 
   Size:         49     575
 
EaeI y'GGCC_r

Cuts at:      0      97     447     504     624 
   Size:         97     350      57     120

  Fragments arranged by size: 

                 350     120      97      57
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     194     221     624 
   Size:        194      27     403

  Fragments arranged by size: 

                 403     194      27
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     176     624 
   Size:        176     448
 
EcoO109I rG'GnC_Cy

Cuts at:      0       5      14     141     142     624 
   Size:          5       9     127       1     482

  Fragments arranged by size: 

                 482     127       9       5       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      53      85     624 
   Size:         53      32     539

  Fragments arranged by size: 

                 539      53      32
 
HindIII A'AGCT_T

Cuts at:      0     380     439     624 
   Size:        380      59     185

  Fragments arranged by size: 

                 380     185      59
 
MscI TGG'CCA

Cuts at:      0     449     506     624 
   Size:        449      57     118

  Fragments arranged by size: 

                 449     118      57
 
PasI CC'CwG_GG

Cuts at:      0       9      85     624 
   Size:          9      76     539

  Fragments arranged by size: 

                 539      76       9
 
PspOMI G'GGCC_C

Cuts at:      0       5      14     141     624 
   Size:          5       9     127     483

  Fragments arranged by size: 

                 483     127       9       5
 
PstI C_TGCA'G

Cuts at:      0     341     624 
   Size:        341     283
 
SfcI C'TryA_G

Cuts at:      0     239     337     624 
   Size:        239      98     287

  Fragments arranged by size: 

                 287     239      98
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      13     624 
   Size:         13     611
 
StyI C'CwwG_G

Cuts at:      0     519     624 
   Size:        519     105
 
TatI w'GTAC_w

Cuts at:      0     404     624 
   Size:        404     220
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     274     624 
   Size:        274     350
 

 Enzymes that do cut:

     AarI     AcuI     AhdI    AlwNI     ApaI     BanI    BanII     BbsI
     BglI    BglII Bme1580I    BmgBI     BmrI     BpmI    BsaHI    BseRI
    BseYI Bsp1286I    BspMI    BsrFI    BsrGI    BstYI   Bsu36I     BtgI
    BtgZI     EaeI  Eco57MI    EcoNI EcoO109I    Hin4I  HindIII     MscI
     PasI   PspOMI     PstI     SfcI     SfiI     StyI     TatI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BlpI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
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