Restriction map for pF1KB8450
##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AvrII       BalI        
BbeI        BbrPI       BbsI        BcgI        BciVI       
BfaI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BsiWI       
BspDI       BspLU11I    BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     CelII       ClaI        CpoI        DraI        
DrdI        EagI        Eam1105I    EclXI       Eco52I      
EcoO65I     EcoRI       FseI        HgaI        Hin1I       
HpaI        KasI        KpnI        KspI        MaeI        
MfeI        MluI        MluNI       MscI        MunI        
NarI        NheI        NotI        NruI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SspI        
StuI        SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AccII           6  CGCG                 CG^CG
AccIII       1309  TCCGGA               T^CCGGA
AocI          849  CCTAAGG              CC^T[ACGT]AGG
Aor51HI      1505  AGCGCT               AGC^GCT
ApaI         1445  GGGCCC               GGGCC^C
AspI          712  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1898  CCCGGG               C^[CT]CG[GA]G
BamHI        1260  GGATCC               G^GATCC
BanI          390  GGCACC               G^G[CT][GA]CC
BbvCI         795  CCTCAGC              CCTCAGC(-5/-2)
BclI          308  TGATCA               T^GATCA
BglII         971  AGATCT               A^GATCT
BsaBI         561  GATGCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1102  GAGACC               GGTCTC(1/5)
BseAI        1309  TCCGGA               T^CCGGA
BsgI          340  GTGCAG               GTGCAG(16/14)
BsiEI        1494  CGACCG               CG[GA][CT]^CG
BsoBI        1898  CCCGGG               C^[CT]CG[GA]G
Bsp1407I     1184  TGTACA               T^GTACA
BspEI        1309  TCCGGA               T^CCGGA
BspHI        1073  TCATGA               T^CATGA
BsrBI         930  CCGCTC               CCGCTC(-3/-3)
BsrDI          54  CATTGC               GCAATG(2/0)
BsrFI         465  GCCGGC               [GA]^CCGG[CT]
BsrGI        1184  TGTACA               T^GTACA
BstUI           6  CGCG                 CG^CG
Bsu36I        849  CCTAAGG              CC^T[ACGT]AGG
Cfr10I        465  GCCGGC               [GA]^CCGG[CT]
DraIII       1870  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI         1432  GAAGAG               CTCTTC(1/4)
Eco47III     1505  AGCGCT               AGC^GCT
Eco81I        849  CCTAAGG              CC^T[ACGT]AGG
EcoNI        1008  CCTGGGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        1582  GATATC               GAT^ATC
EcoT22I       326  ATGCAT               ATGCA^T
FbaI          308  TGATCA               T^GATCA
HaeII        1505  AGCGCT               [GA]GCGC^[CT]
HindIII      1681  AAGCTT               A^AGCTT
Ksp632I      1432  GAAGAG               CTCTTC(1/4)
MamI          561  GATGCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         1309  TCCGGA               T^CCGGA
MseI         1084  TTAA                 T^TAA
MvnI            6  CGCG                 CG^CG
NaeI          465  GCCGGC               GCC^GGC
NdeI          329  CATATG               CA^TATG
NgoMIV        465  GCCGGC               G^CCGGC
NsiI          326  ATGCAT               ATGCA^T
PflFI         712  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI         627  GACCCCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         1073  TCATGA               T^CATGA
SmaI         1898  CCCGGG               CCC^GGG
SmlI         1165  CTCAAG               C^T[CT][GA]AG
SspBI        1184  TGTACA               T^GTACA
Tru9I        1084  TTAA                 T^TAA
Tth111I       712  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        1898  CCCGGG               C^CCGGG
XmaI         1898  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AlwNI         416  CAGAGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1634  CAGATCCTG           
AspHI         222  GTGCTC               G[AT]GC[AT]^C
             1162  GTGCTC              
BanII         901  GAGCCC               G[GA]GC[CT]^C
             1445  GGGCCC              
BsiHKAI       222  GTGCTC               G[AT]GC[AT]^C
             1162  GTGCTC              
BspMI        1205  ACCTGC               ACCTGC(4/8)
             1623  GCAGGT              
EaeI          467  CGGCCA               [CT]^GGCC[GA]
             1736  TGGCCG              
HincII         85  GTCAAC               GT[CT]^[GA]AC
              553  GTCAAC              
HindII         85  GTCAAC               GT[CT]^[GA]AC
              553  GTCAAC              
MaeII         843  ACGT                 A^CGT
             1676  ACGT                
NcoI          996  CCATGG               C^CATGG
             1451  CCATGG              
PflMI          37  CCAGGCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              723  CCAACGCCTGG         
PpuMI         625  GGGACCC              [GA]G^G[AT]CC[CT]
             1004  AGGTCCT             
PstI         1749  CTGCAG               CTGCA^G
             1847  CTGCAG              
SfcI         1749  CTGCAG               C^T[GA][CT]AG
             1847  CTGCAG              
SphI          345  GCATGC               GCATG^C
             1880  GCATGC              
Van91I         37  CCAGGCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              723  CCAACGCCTGG         
XcmI           61  CCAAGCCAGATATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              812  CCAAGGAGAATCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        Asp700I     
AvaII       AviII       BbvI        BcnI        BmyI        
BpmI        BsaJI       BsaWI       BseRI       BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsmI        
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        FspI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeIII      MboI        
MboII       MflI        MnlI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
StyI        TaqI        TfiI        TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       XmnI        
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