Restriction map for pF1KB8450
 (Linear) (Six-base) MAPSORT of: KIBB8450.seq  Check: 9578  from: 1  to: 1914



KIBB8450.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1614    1914 
   Size:       1614     300
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     497     676    1258    1914 
   Size:        497     179     582     656

  Fragments arranged by size: 

                 656     582     497     179
 
AfeI AGC'GCT

Cuts at:      0    1507    1914 
   Size:       1507     407
 
AleI CACnn'nnGTG

Cuts at:      0     535    1516    1914 
   Size:        535     981     398

  Fragments arranged by size: 

                 981     535     398
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1646    1858    1914 
   Size:       1646     212      56

  Fragments arranged by size: 

                1646     212      56
 
AlwNI CAG_nnn'CTG

Cuts at:      0     421    1639    1914 
   Size:        421    1218     275

  Fragments arranged by size: 

                1218     421     275
 
ApaI G_GGCC'C

Cuts at:      0    1449    1914 
   Size:       1449     465
 
AvaI C'yCGr_G

Cuts at:      0    1898    1914 
   Size:       1898      16
 
BamHI G'GATC_C

Cuts at:      0    1260    1914 
   Size:       1260     654
 
BanI G'GyrC_C

Cuts at:      0     390    1914 
   Size:        390    1524
 
BanII G_rGCy'C

Cuts at:      0     905    1449    1914 
   Size:        905     544     465

  Fragments arranged by size: 

                 905     544     465
 
BbvCI CC'TCA_GC

Cuts at:      0     796    1914 
   Size:        796    1118
 
BclI T'GATC_A

Cuts at:      0     308    1914 
   Size:        308    1606
 
BfrBI ATG'CAT

Cuts at:      0     328    1914 
   Size:        328    1586
 
BglII A'GATC_T

Cuts at:      0     971    1914 
   Size:        971     943
 
Bme1580I G_kGCm'C

Cuts at:      0    1234    1449    1914 
   Size:       1234     215     465

  Fragments arranged by size: 

                1234     465     215
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     201    1914 
   Size:        201    1713
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     426    1827    1914 
   Size:        426    1401      87

  Fragments arranged by size: 

                1401     426      87
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     634     766     844    1777    1876    1914 
   Size:         48     586     132      78     933      99      38

  Fragments arranged by size: 

                 933     586     132      99      78      48      38
 
Bpu10I CC'TnA_GC

Cuts at:      0     796    1914 
   Size:        796    1118
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1150    1914 
   Size:       1150     764
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1096    1914 
   Size:       1096     818
 
BsaBI GATnn'nnATC

Cuts at:      0     565    1914 
   Size:        565    1349
 
BsaWI w'CCGG_w

Cuts at:      0     113     557    1309    1914 
   Size:        113     444     752     605

  Fragments arranged by size: 

                 752     605     444     113
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     253     283     703     733     853     883    1914 
   Size:        253      30     420      30     120      30    1031

  Fragments arranged by size: 

                1031     420     253     120      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     667     752     852    1114    1873    1914 
   Size:        667      85     100     262     759      41

  Fragments arranged by size: 

                 759     667     262     100      85      41
 
BseYI C'CCAG_C

Cuts at:      0     256     886    1830    1914 
   Size:        256     630     944      84

  Fragments arranged by size: 

                 944     630     256      84
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     361    1914 
   Size:        361    1553
 
BsiEI CG_ry'CG

Cuts at:      0    1497    1914 
   Size:       1497     417
 
BsiHKAI G_wGCw'C

Cuts at:      0     226    1166    1914 
   Size:        226     940     748

  Fragments arranged by size: 

                 940     748     226
 
BsmI GAATG_Cn'

Cuts at:      0     130     780    1777    1914 
   Size:        130     650     997     137

  Fragments arranged by size: 

                 997     650     137     130
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     723     834    1567    1914 
   Size:        723     111     733     347

  Fragments arranged by size: 

                 733     723     347     111
 
Bsp1286I G_dGCh'C

Cuts at:      0     226     905    1166    1234    1449    1914 
   Size:        226     679     261      68     215     465

  Fragments arranged by size: 

                 679     465     261     226     215      68
 
BspEI T'CCGG_A

Cuts at:      0    1309    1914 
   Size:       1309     605
 
BspHI T'CATG_A

Cuts at:      0    1073    1914 
   Size:       1073     841
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1214    1614    1914 
   Size:       1214     400     300

  Fragments arranged by size: 

                1214     400     300
 
BsrBI CCG'CTC

Cuts at:      0     932    1914 
   Size:        932     982
 
BsrDI GCAATG_nn'

Cuts at:      0      53    1914 
   Size:         53    1861
 
BsrFI r'CCGG_y

Cuts at:      0     465    1914 
   Size:        465    1449
 
BsrGI T'GTAC_A

Cuts at:      0    1184    1914 
   Size:       1184     730
 
BstYI r'GATC_y

Cuts at:      0     971    1260    1350    1635    1914 
   Size:        971     289      90     285     279

  Fragments arranged by size: 

                 971     289     285     279      90
 
Bsu36I CC'TnA_GG

Cuts at:      0     850    1914 
   Size:        850    1064
 
BtgI C'CryG_G

Cuts at:      0     503     996    1055    1451    1914 
   Size:        503     493      59     396     463

  Fragments arranged by size: 

                 503     493     463     396      59
 
DraIII CAC_nnn'GTG

Cuts at:      0    1875    1914 
   Size:       1875      39
 
EaeI y'GGCC_r

Cuts at:      0     467    1736    1914 
   Size:        467    1269     178

  Fragments arranged by size: 

                1269     467     178
 
EarI CTCTTCn'nnn_

Cuts at:      0    1427    1914 
   Size:       1427     487
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     497     634     676     766     844    1258    1777 
   Size:         48     449     137      42      90      78     414     519

Cuts at:   1777    1876    1914 
   Size:         99      38

  Fragments arranged by size: 

                 519     449     414     137      99      90      78      48
                  42      38
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1012    1914 
   Size:       1012     902
 
EcoO109I rG'GnC_Cy

Cuts at:      0     241     626    1005    1445    1446    1806    1914 
   Size:        241     385     379     440       1     360     108

  Fragments arranged by size: 

                 440     385     379     360     241     108       1
 
EcoRV GAT'ATC

Cuts at:      0    1584    1914 
   Size:       1584     330
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1038    1914 
   Size:       1038     876
 
FspI TGC'GCA

Cuts at:      0    1141    1252    1486    1914 
   Size:       1141     111     234     428

  Fragments arranged by size: 

                1141     428     234     111
 
HaeII r_GCGC'y

Cuts at:      0    1509    1914 
   Size:       1509     405
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     703     735    1342    1374    1573    1605    1914 
   Size:        703      32     607      32     199      32     309

  Fragments arranged by size: 

                 703     607     309     199      32      32      32
 
HincII GTy'rAC

Cuts at:      0      87     555    1914 
   Size:         87     468    1359

  Fragments arranged by size: 

                1359     468      87
 
HindIII A'AGCT_T

Cuts at:      0    1681    1914 
   Size:       1681     233
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     545    1361    1844    1914 
   Size:        545     816     483      70

  Fragments arranged by size: 

                 816     545     483      70
 
MslI CAynn'nnrTG

Cuts at:      0     460     535    1157    1177    1516    1914 
   Size:        460      75     622      20     339     398

  Fragments arranged by size: 

                 622     460     398     339      75      20
 
MspA1I CmG'CkG

Cuts at:      0     435     696     738    1734    1842    1914 
   Size:        435     261      42     996     108      72

  Fragments arranged by size: 

                 996     435     261     108      72      42
 
NaeI GCC'GGC

Cuts at:      0     467    1914 
   Size:        467    1447
 
NcoI C'CATG_G

Cuts at:      0     996    1451    1914 
   Size:        996     455     463

  Fragments arranged by size: 

                 996     463     455
 
NdeI CA'TA_TG

Cuts at:      0     330    1914 
   Size:        330    1584
 
NgoMIV G'CCGG_C

Cuts at:      0     465    1914 
   Size:        465    1449
 
NsiI A_TGCA'T

Cuts at:      0     330    1914 
   Size:        330    1584
 
NspI r_CATG'y

Cuts at:      0     349     771    1884    1914 
   Size:        349     422    1113      30

  Fragments arranged by size: 

                1113     422     349      30
 
PasI CC'CwG_GG

Cuts at:      0     598    1914 
   Size:        598    1316
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      43     729    1914 
   Size:         43     686    1185

  Fragments arranged by size: 

                1185     686      43
 
PfoI T'CCnGG_A

Cuts at:      0     611    1914 
   Size:        611    1303
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     828     860    1914 
   Size:        828      32    1054

  Fragments arranged by size: 

                1054     828      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     626    1005    1914 
   Size:        626     379     909

  Fragments arranged by size: 

                 909     626     379
 
PshAI GACnn'nnGTC

Cuts at:      0     631    1914 
   Size:        631    1283
 
PspOMI G'GGCC_C

Cuts at:      0    1445    1914 
   Size:       1445     469
 
PstI C_TGCA'G

Cuts at:      0    1753    1851    1914 
   Size:       1753      98      63

  Fragments arranged by size: 

                1753      98      63
 
PvuII CAG'CTG

Cuts at:      0     435     696     738    1914 
   Size:        435     261      42    1176

  Fragments arranged by size: 

                1176     435     261      42
 
SanDI GG'GwC_CC

Cuts at:      0     626    1914 
   Size:        626    1288
 
SfcI C'TryA_G

Cuts at:      0    1749    1847    1914 
   Size:       1749      98      67

  Fragments arranged by size: 

                1749      98      67
 
SmaI CCC'GGG

Cuts at:      0    1900    1914 
   Size:       1900      14
 
SmlI C'TyrA_G

Cuts at:      0    1165    1914 
   Size:       1165     749
 
SphI G_CATG'C

Cuts at:      0     349    1884    1914 
   Size:        349    1535      30

  Fragments arranged by size: 

                1535     349      30
 
StyI C'CwwG_G

Cuts at:      0     182     812     996    1451    1914 
   Size:        182     630     184     455     463

  Fragments arranged by size: 

                 630     463     455     184     182
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     209     290     408    1914 
   Size:        209      81     118    1506

  Fragments arranged by size: 

                1506     209     118      81
 
TatI w'GTAC_w

Cuts at:      0    1184    1217    1914 
   Size:       1184      33     697

  Fragments arranged by size: 

                1184     697      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1698    1730    1914 
   Size:       1698      32     184

  Fragments arranged by size: 

                1698     184      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     715    1914 
   Size:        715    1199
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      68     819    1914 
   Size:         68     751    1095

  Fragments arranged by size: 

                1095     751      68
 
XmaI C'CCGG_G

Cuts at:      0    1898    1914 
   Size:       1898      16
 
XmnI GAAnn'nnTTC

Cuts at:      0     941    1018    1684    1914 
   Size:        941      77     666     230

  Fragments arranged by size: 

                 941     666     230      77
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AleI     AlfI    AlwNI     ApaI     AvaI
    BamHI     BanI    BanII    BbvCI     BclI    BfrBI    BglII Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BstYI   Bsu36I     BtgI   DraIII     EaeI     EarI  Eco57MI    EcoNI
 EcoO109I    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     MmeI     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NsiI
     NspI     PasI    PflMI     PfoI     PpiI    PpuMI    PshAI   PspOMI
     PstI    PvuII    SanDI     SfcI     SmaI     SmlI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
     BbeI     BbsI     BcgI    BciVI     BglI     BlpI    BmgBI     BmtI
    BsaAI    BsaHI    BsiWI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI
     EagI     EciI  EcoICRI    EcoRI     FseI    FspAI     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NarI     NheI     NotI     NruI
     PacI     PciI     PmeI     PmlI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI
     StuI     SwaI     XbaI     XhoI     ZraI
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