Restriction map for pF1KB7316
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaLI       AscI        Asp718I     
AspEI       AvaI        AviII       AvrII       BamHI       
BanI        BbeI        BcgI        BclI        BfrI        
BglI        BlnI        BsaHI       BsaWI       BseAI       
BsiWI       BsoBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BspMI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstZ17I     CfoI        
Cfr10I      ClaI        CpoI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO65I     
EcoRI       EcoT22I     FbaI        FseI        FspI        
HaeII       HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MfeI        MluI        MroI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmeI        PshAI       PvuI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SpeI        SphI        Sse8387I    SspBI       StuI        
SwaI        Van91I      XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AclI          391  AACGTT               AA^CGTT
AocI         3088  CCTCAGG              CC^T[ACGT]AGG
Asp700I      2233  GAAGACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1966  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          846  TGGCCA               TGG^CCA
BbrPI        1091  CACGTG               CAC^GTG
BbvCI        1768  CCTCAGC              CCTCAGC(-5/-2)
BcnI         2460  CCCGG                CC^[GC]GG
BfaI         3134  CTAG                 C^TAG
BglII         419  AGATCT               A^GATCT
BlpI         2788  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2788  GCTGAGC              GC^T[ACGT]AGC
BsaBI        1253  GATCACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          691  GGTCTC               GGTCTC(1/5)
BsgI         2906  CTGCAC               GTGCAG(16/14)
BsiEI        1996  CGGTCG               CG[GA][CT]^CG
BsmBI        1991  CGTCTC               CGTCTC(1/5)
BsmI         1230  GAATGC               GAATGC(1/-1)
BspHI         150  TCATGA               T^CATGA
BsrBI         498  CCGCTC               CCGCTC(-3/-3)
BstXI         870  CCATCTTGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       3088  CCTCAGG              CC^T[ACGT]AGG
CelII        2788  GCTGAGC              GC^T[ACGT]AGC
DraI         1035  TTTAAA               TTT^AAA
DraIII        311  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          222  CCGTGG               C^C[GA][CT]GG
EaeI          846  TGGCCA               [CT]^GGCC[GA]
Eco81I       3088  CCTCAGG              CC^T[ACGT]AGG
EcoNI        2983  CCTGTAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       629  CCTTGG               C^C[AT][AT]GG
FauI         3076  GCGGG                CCCGC(4/6)
HapII        2461  CCGG                 C^CGG
HgaI         2793  GCGTC                GACGC(5/10)
HpaII        2461  CCGG                 C^CGG
MaeI         3134  CTAG                 C^TAG
MamI         1253  GATCACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         846  TGGCCA               TGG^CCA
MscI          846  TGGCCA               TGG^CCA
MspI         2461  CCGG                 C^CGG
NciI         2460  CCCGG                CC^[GC]GG
PflFI        1966  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1091  CACGTG               CAC^GTG
PmlI         1091  CACGTG               CAC^GTG
PpuMI         256  AGGTCCC              [GA]G^G[AT]CC[CT]
Psp1406I      391  AACGTT               AA^CGTT
PvuII        1340  CAGCTG               CAG^CTG
RcaI          150  TCATGA               T^CATGA
SacI          547  GAGCTC               GAGCT^C
StyI          629  CCTTGG               C^C[AT][AT]GG
Tth111I      1966  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          641  CCATTCATTTTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         2233  GAAGACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

ApaI         1086  GGGCCC               GGGCC^C
             2457  GGGCCC              
AspHI         547  GAGCTC               G[AT]GC[AT]^C
             2678  GTGCTC              
AvaII         257  GGTCC                G^G[AT]CC
             3254  GGTCC               
BbvI         1434  GCAGC                GCAGC(8/12)
             3049  GCTGC               
BsaJI         222  CCGTGG               C^C[ACGT][ACGT]GG
              629  CCTTGG              
BsiHKAI       547  GAGCTC               G[AT]GC[AT]^C
             2678  GTGCTC              
BstYI         419  AGATCT               [GA]^GATC[CT]
             3136  AGATCC              
Cac8I        2433  GCGAGC               GC[ACGT]^[ACGT]GC
             2995  GCAGGC              
DraII         256  AGGTCCC              [GA]G^G[ACGT]CC[CT]
             1611  GGGGCCT             
EcoO109I      256  AGGTCCC              [GA]G^G[ACGT]CC[CT]
             1611  GGGGCCT             
EcoRV         989  GATATC               GAT^ATC
             2123  GATATC              
MflI          419  AGATCT               [GA]^GATC[CT]
             3136  AGATCC              
PstI         2207  CTGCAG               CTGCA^G
             3050  CTGCAG              
SexAI        2076  ACCAGGT              A^CC[AT]GGT
             2423  ACCTGGT             
SmlI          767  CTCAAG               C^T[CT][GA]AG
             2948  CTCAAG              
SnaBI          13  TACGTA               TAC^GTA
              530  TACGTA              
SspI          907  AATATT               AAT^ATT
             2359  AATATT              
TseI         1434  GCAGC                G^C[AT]GC
             3049  GCTGC               
XhoII         419  AGATCT               [GA]^GATC[CT]
             3136  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AseI        AsnI        BanII       
BbsI        BciVI       BmyI        BpmI        BpuAI       
BsaAI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cfr13I      DdeI        DpnII       EarI        
EcoRII      Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspA1I      MvaI        MwoI        NdeII       NlaIII      
NlaIV       PleI        PshBI       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TaqI        
TfiI        Tru9I       Tsp45I      Tsp509I     TspRI       
TthHB8I     
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