Restriction map for
pF1KB7316
(Linear) (Six-base) MAPSORT of: KIBB7316.seq Check: 8262 from: 1 to: 3291
KIBB7316.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AclI AA'CG_TT
Cuts at: 0 392 3291
Size: 392 2899
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1175 2819 3291
Size: 1175 1644 472
Fragments arranged by size:
1644 1175 472
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1515 1547 3291
Size: 1515 32 1744
Fragments arranged by size:
1744 1515 32
AlwNI CAG_nnn'CTG
Cuts at: 0 84 2539 2788 2821 3291
Size: 84 2455 249 33 470
Fragments arranged by size:
2455 470 249 84 33
ApaI G_GGCC'C
Cuts at: 0 1090 2461 3291
Size: 1090 1371 830
Fragments arranged by size:
1371 1090 830
ApoI r'AATT_y
Cuts at: 0 72 296 336 370 426 1464 1650 1830
Size: 72 224 40 34 56 1038 186 180
Cuts at: 1830 1874 2184 2493 3111 3291
Size: 44 310 309 618 180
Fragments arranged by size:
1038 618 310 309 224 186 180 180
72 56 44 40 34
AseI AT'TA_AT
Cuts at: 0 1331 1385 1682 2506 3026 3188 3291
Size: 1331 54 297 824 520 162 103
Fragments arranged by size:
1331 824 520 297 162 103 54
BanII G_rGCy'C
Cuts at: 0 147 551 718 1090 1843 2461 3291
Size: 147 404 167 372 753 618 830
Fragments arranged by size:
830 753 618 404 372 167 147
BbsI GAAGACnn'nnnn_
Cuts at: 0 2240 2386 3230 3291
Size: 2240 146 844 61
Fragments arranged by size:
2240 844 146 61
BbvCI CC'TCA_GC
Cuts at: 0 1769 3291
Size: 1769 1522
BciVI GTATCCnnnnn_n'
Cuts at: 0 731 2193 2575 3291
Size: 731 1462 382 716
Fragments arranged by size:
1462 731 716 382
BglII A'GATC_T
Cuts at: 0 419 3291
Size: 419 2872
BlpI GC'TnA_GC
Cuts at: 0 2789 3291
Size: 2789 502
Bme1580I G_kGCm'C
Cuts at: 0 1090 2461 3291
Size: 1090 1371 830
Fragments arranged by size:
1371 1090 830
BmrI ACTGGGnnnn_n'
Cuts at: 0 2521 2776 3291
Size: 2521 255 515
Fragments arranged by size:
2521 515 255
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3180 3291
Size: 3180 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 627 732 2040 2918 3107 3291
Size: 398 229 105 1308 878 189 184
Fragments arranged by size:
1308 878 398 229 189 184 105
Bpu10I CC'TnA_GC
Cuts at: 0 1769 3291
Size: 1769 1522
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 752 2933 3291
Size: 752 2181 358
Fragments arranged by size:
2181 752 358
BsaI GGTCTCn'nnnn_
Cuts at: 0 697 3291
Size: 697 2594
BsaAI yAC'GTr
Cuts at: 0 15 532 1093 3291
Size: 15 517 561 2198
Fragments arranged by size:
2198 561 517 15
BsaBI GATnn'nnATC
Cuts at: 0 1257 3291
Size: 1257 2034
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 137 995 1921 3260 3291
Size: 137 858 926 1339 31
Fragments arranged by size:
1339 926 858 137 31
BseYI C'CCAG_C
Cuts at: 0 1245 2880 3291
Size: 1245 1635 411
Fragments arranged by size:
1635 1245 411
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2891 3291
Size: 2891 400
BsiEI CG_ry'CG
Cuts at: 0 1999 3291
Size: 1999 1292
BsiHKAI G_wGCw'C
Cuts at: 0 551 2682 3291
Size: 551 2131 609
Fragments arranged by size:
2131 609 551
BsmI GAATG_Cn'
Cuts at: 0 1236 3291
Size: 1236 2055
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1997 3291
Size: 1997 1294
Bsp1286I G_dGCh'C
Cuts at: 0 147 551 718 1090 1843 2461 2682 3291
Size: 147 404 167 372 753 618 221 609
Fragments arranged by size:
753 618 609 404 372 221 167 147
BspHI T'CATG_A
Cuts at: 0 150 3291
Size: 150 3141
BsrBI CCG'CTC
Cuts at: 0 500 3291
Size: 500 2791
BstXI CCAn_nnnn'nTGG
Cuts at: 0 877 3291
Size: 877 2414
BstYI r'GATC_y
Cuts at: 0 419 3136 3291
Size: 419 2717 155
Fragments arranged by size:
2717 419 155
Bsu36I CC'TnA_GG
Cuts at: 0 3089 3291
Size: 3089 202
BtgI C'CryG_G
Cuts at: 0 222 3291
Size: 222 3069
BtsI GCAGTG_nn'
Cuts at: 0 2540 2682 3291
Size: 2540 142 609
Fragments arranged by size:
2540 609 142
DraI TTT'AAA
Cuts at: 0 1037 3291
Size: 1037 2254
DraIII CAC_nnn'GTG
Cuts at: 0 316 3291
Size: 316 2975
EaeI y'GGCC_r
Cuts at: 0 846 3291
Size: 846 2445
EarI CTCTTCn'nnn_
Cuts at: 0 692 2461 3050 3291
Size: 692 1769 589 241
Fragments arranged by size:
1769 692 589 241
EcoICRI GAG'CTC
Cuts at: 0 549 3291
Size: 549 2742
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 627 732 1175 2040 2819 2918 3107
Size: 398 229 105 443 865 779 99 189
Cuts at: 3107 3291
Size: 184
Fragments arranged by size:
865 779 443 398 229 189 184 105
99
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2987 3291
Size: 2987 304
EcoO109I rG'GnC_Cy
Cuts at: 0 257 1612 3291
Size: 257 1355 1679
Fragments arranged by size:
1679 1355 257
EcoRV GAT'ATC
Cuts at: 0 991 2125 3291
Size: 991 1134 1166
Fragments arranged by size:
1166 1134 991
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 613 799 1124 3291
Size: 613 186 325 2167
Fragments arranged by size:
2167 613 325 186
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2917 2949 3211 3243 3291
Size: 2917 32 262 32 48
Fragments arranged by size:
2917 262 48 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 1036 1979 2529 3291
Size: 257 779 943 550 762
Fragments arranged by size:
943 779 762 550 257
MscI TGG'CCA
Cuts at: 0 848 3291
Size: 848 2443
MslI CAynn'nnrTG
Cuts at: 0 875 2242 2527 3291
Size: 875 1367 285 764
Fragments arranged by size:
1367 875 764 285
MspA1I CmG'CkG
Cuts at: 0 1245 1342 1437 3291
Size: 1245 97 95 1854
Fragments arranged by size:
1854 1245 97 95
PfoI T'CCnGG_A
Cuts at: 0 34 1062 3291
Size: 34 1028 2229
Fragments arranged by size:
2229 1028 34
PmlI CAC'GTG
Cuts at: 0 1093 3291
Size: 1093 2198
PpuMI rG'GwC_Cy
Cuts at: 0 257 3291
Size: 257 3034
PspOMI G'GGCC_C
Cuts at: 0 1086 2457 3291
Size: 1086 1371 834
Fragments arranged by size:
1371 1086 834
PstI C_TGCA'G
Cuts at: 0 2211 3054 3291
Size: 2211 843 237
Fragments arranged by size:
2211 843 237
PvuII CAG'CTG
Cuts at: 0 1342 3291
Size: 1342 1949
SacI G_AGCT'C
Cuts at: 0 551 3291
Size: 551 2740
SexAI A'CCwGG_T
Cuts at: 0 2076 2423 3291
Size: 2076 347 868
Fragments arranged by size:
2076 868 347
SfcI C'TryA_G
Cuts at: 0 2207 2984 3050 3291
Size: 2207 777 66 241
Fragments arranged by size:
2207 777 241 66
SmlI C'TyrA_G
Cuts at: 0 767 2948 3291
Size: 767 2181 343
Fragments arranged by size:
2181 767 343
SnaBI TAC'GTA
Cuts at: 0 15 532 3291
Size: 15 517 2759
Fragments arranged by size:
2759 517 15
SspI AAT'ATT
Cuts at: 0 909 2361 3291
Size: 909 1452 930
Fragments arranged by size:
1452 930 909
StyI C'CwwG_G
Cuts at: 0 629 3291
Size: 629 2662
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1985 2868 3291
Size: 1985 883 423
Fragments arranged by size:
1985 883 423
TatI w'GTAC_w
Cuts at: 0 1425 3291
Size: 1425 1866
Tth111I GACn'n_nGTC
Cuts at: 0 1969 3291
Size: 1969 1322
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 648 3291
Size: 648 2643
XmnI GAAnn'nnTTC
Cuts at: 0 2237 3291
Size: 2237 1054
Enzymes that do cut:
AclI AcuI AloI AlwNI ApaI ApoI AseI BanII
BbsI BbvCI BciVI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrBI
BstXI BstYI Bsu36I BtgI BtsI DraI DraIII EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI Hin4I
MmeI MscI MslI MspA1I PfoI PmlI PpuMI PspOMI
PstI PvuII SacI SexAI SfcI SmlI SnaBI SspI
StyI TaqII TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AleI AlfI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BcgI BclI BfrBI BglI
BmgBI BmtI BsaHI BsaWI BsaXI BsiWI BspEI BspMI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgZI ClaI CspCI DrdI EagI EciI EcoRI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SpeI SphI SrfI StuI
SwaI TstI XbaI XhoI XmaI ZraI