Restriction map for pF1KB6333
 (Linear) (Six-base) MAPSORT of: KIBB6333.seq  Check: 4672  from: 1  to: 783



KIBB6333.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     105     460     631     783 
   Size:        105     355     171     152

  Fragments arranged by size: 

                 355     171     152     105
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     269     337     496     783 
   Size:        269      68     159     287

  Fragments arranged by size: 

                 287     269     159      68
 
AflIII A'CryG_T

Cuts at:      0     545     783 
   Size:        545     238
 
AleI CACnn'nnGTG

Cuts at:      0     733     769     783 
   Size:        733      36      14

  Fragments arranged by size: 

                 733      36      14
 
AlwNI CAG_nnn'CTG

Cuts at:      0     385     783 
   Size:        385     398
 
ApaI G_GGCC'C

Cuts at:      0     646     783 
   Size:        646     137
 
ApaLI G'TGCA_C

Cuts at:      0     163     783 
   Size:        163     620
 
AvaI C'yCGr_G

Cuts at:      0     627     783 
   Size:        627     156
 
BanII G_rGCy'C

Cuts at:      0     395     646     783 
   Size:        395     251     137

  Fragments arranged by size: 

                 395     251     137
 
BbsI GAAGACnn'nnnn_

Cuts at:      0       9     257     783 
   Size:          9     248     526

  Fragments arranged by size: 

                 526     248       9
 
BglI GCCn_nnn'nGGC

Cuts at:      0     714     783 
   Size:        714      69
 
Bme1580I G_kGCm'C

Cuts at:      0     167     646     705     783 
   Size:        167     479      59      78

  Fragments arranged by size: 

                 479     167      78      59
 
BmgBI CAC'GTC

Cuts at:      0      38     546     680     783 
   Size:         38     508     134     103

  Fragments arranged by size: 

                 508     134     103      38
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     165     451     783 
   Size:        165     286     332

  Fragments arranged by size: 

                 332     286     165
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     333     783 
   Size:        333     450
 
BsaWI w'CCGG_w

Cuts at:      0     746     783 
   Size:        746      37
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     502     783 
   Size:        502     281
 
BseYI C'CCAG_C

Cuts at:      0      83     222     467     783 
   Size:         83     139     245     316

  Fragments arranged by size: 

                 316     245     139      83
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      35     783 
   Size:         35     748
 
BsiHKAI G_wGCw'C

Cuts at:      0     167     395     783 
   Size:        167     228     388

  Fragments arranged by size: 

                 388     228     167
 
BsmI GAATG_Cn'

Cuts at:      0      99     783 
   Size:         99     684
 
Bsp1286I G_dGCh'C

Cuts at:      0     167     395     646     705     783 
   Size:        167     228     251      59      78

  Fragments arranged by size: 

                 251     228     167      78      59
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     105     428     460     631     783 
   Size:        105     323      32     171     152

  Fragments arranged by size: 

                 323     171     152     105      32
 
BsrBI CCG'CTC

Cuts at:      0     297     783 
   Size:        297     486
 
BsrGI T'GTAC_A

Cuts at:      0     725     783 
   Size:        725      58
 
BssSI C'ACGA_G

Cuts at:      0     131     783 
   Size:        131     652
 
BstEII G'GTnAC_C

Cuts at:      0     573     783 
   Size:        573     210
 
Bsu36I CC'TnA_GG

Cuts at:      0     331     462     783 
   Size:        331     131     321

  Fragments arranged by size: 

                 331     321     131
 
BtsI GCAGTG_nn'

Cuts at:      0     192     696     783 
   Size:        192     504      87

  Fragments arranged by size: 

                 504     192      87
 
ClaI AT'CG_AT

Cuts at:      0     324     783 
   Size:        324     459
 
EaeI y'GGCC_r

Cuts at:      0     773     783 
   Size:        773      10
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     361     783 
   Size:        361     422
 
EcoICRI GAG'CTC

Cuts at:      0     393     783 
   Size:        393     390
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     269     333     337     496     783 
   Size:        269      64       4     159     287

  Fragments arranged by size: 

                 287     269     159      64       4
 
EcoO109I rG'GnC_Cy

Cuts at:      0       6     177     642     783 
   Size:          6     171     465     141

  Fragments arranged by size: 

                 465     171     141       6
 
FspI TGC'GCA

Cuts at:      0     554     783 
   Size:        554     229
 
MscI TGG'CCA

Cuts at:      0     775     783 
   Size:        775       8
 
MslI CAynn'nnrTG

Cuts at:      0     733     769     783 
   Size:        733      36      14

  Fragments arranged by size: 

                 733      36      14
 
MspA1I CmG'CkG

Cuts at:      0     187     783 
   Size:        187     596
 
PpuMI rG'GwC_Cy

Cuts at:      0       6     177     783 
   Size:          6     171     606

  Fragments arranged by size: 

                 606     171       6
 
PspOMI G'GGCC_C

Cuts at:      0     642     783 
   Size:        642     141
 
PvuII CAG'CTG

Cuts at:      0     187     783 
   Size:        187     596
 
SacI G_AGCT'C

Cuts at:      0     395     783 
   Size:        395     388
 
SanDI GG'GwC_CC

Cuts at:      0       6     783 
   Size:          6     777
 
StuI AGG'CCT

Cuts at:      0     717     783 
   Size:        717      66
 
StyI C'CwwG_G

Cuts at:      0     731     783 
   Size:        731      52
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     442     783 
   Size:        442     341
 
TatI w'GTAC_w

Cuts at:      0     725     783 
   Size:        725      58
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      44      76     783 
   Size:         44      32     707

  Fragments arranged by size: 

                 707      44      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     325     783 
   Size:        325     458
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AleI    AlwNI     ApaI    ApaLI     AvaI
    BanII     BbsI     BglI Bme1580I    BmgBI     BmrI     BpmI    BsaWI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI
    BsrGI    BssSI   BstEII   Bsu36I     BtsI     ClaI     EaeI     EciI
  EcoICRI  Eco57MI EcoO109I     FspI     MscI     MslI   MspA1I    PpuMI
   PspOMI    PvuII     SacI    SanDI     StuI     StyI    TaqII     TatI
     TstI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BsrDI    BsrFI   BssHII   BstAPI    BstBI    BstXI    BstYI  BstZ17I
     BtgI    BtgZI    CspCI     DraI   DraIII     DrdI     EagI     EarI
    EcoNI    EcoRI    EcoRV     FalI     FseI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    RsrII    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI
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