Restriction map for pF1KB7330
##################################
# NO CUT ENZYMES (   73 enzymes) #
##################################

AclI        AflII       AgeI        Alw44I      AocI        
Aor51HI     ApaLI       AseI        AsnI        AspI        
AvrII       BalI        BamHI       BcgI        BciVI       
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsiWI       BsmI        BspDI       BspHI       BspLU11I    
BstBI       Bsu36I      CelII       ClaI        CpoI        
DraI        DraIII      DrdI        Eco47III    Eco81I      
EcoRI       EcoRV       EcoT22I     FseI        HindIII     
HpaI        KspI        MfeI        MluI        MluNI       
MscI        MunI        NdeI        NheI        NruI        
NsiI        NspV        PacI        PflFI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SexAI       SfiI        
SfuI        SnaBI       SpeI        SphI        SspI        
SwaI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AatII        1696  GACGTC               GACGT^C
Acc65I        458  GGTACC               G^GTACC
AccIII        590  TCCGGA               T^CCGGA
AcsI          234  GAATTT               [GA]^AATT[CT]
AhdI         1729  GACATGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         610  CAGATTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          234  GAATTT               [GA]^AATT[CT]
AscI           88  GGCGCGCC             GG^CGCGCC
Asp718I       458  GGTACC               G^GTACC
AspEI        1729  GACATGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        2219  TGCGCA               TGC^GCA
BbrPI        1074  CACGTG               CAC^GTG
BclI          815  TGATCA               T^GATCA
BfaI         1298  CTAG                 C^TAG
BglI         1337  GCCCCCGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI         1960  GAGACC               GGTCTC(1/5)
BsaWI         590  TCCGGA               [AT]^CCGG[AT]
BseAI         590  TCCGGA               T^CCGGA
BseRI        2386  GAGGAG               GAGGAG(10/8)
BsmBI        1238  CGTCTC               CGTCTC(1/5)
Bsp1407I     1112  TGTACA               T^GTACA
BspEI         590  TCCGGA               T^CCGGA
BsrGI        1112  TGTACA               T^GTACA
BssHII         89  GCGCGC               G^CGCGC
Bst1107I       28  GTATAC               GTA^TAC
BstXI        1850  CCAGCATGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         431  GGATCT               [GA]^GATC[CT]
BstZ17I        28  GTATAC               GTA^TAC
Eam1105I     1729  GACATGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        2295  CCTGCGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          815  TGATCA               T^GATCA
FspI         2219  TGCGCA               TGC^GCA
HincII       2038  GTCGAC               GT[CT]^[GA]AC
HindII       2038  GTCGAC               GT[CT]^[GA]AC
KpnI          458  GGTACC               GGTAC^C
MaeI         1298  CTAG                 C^TAG
MflI          431  GGATCT               [GA]^GATC[CT]
MroI          590  TCCGGA               T^CCGGA
MseI         1745  TTAA                 T^TAA
NcoI         1895  CCATGG               C^CATGG
NotI          258  GCGGCCGC             GC^GGCCGC
PaeR7I       1417  CTCGAG               C^TCGAG
PmaCI        1074  CACGTG               CAC^GTG
PmlI         1074  CACGTG               CAC^GTG
SacI          360  GAGCTC               GAGCT^C
SalI         2038  GTCGAC               G^TCGAC
SapI          357  GAAGAGC              GCTCTTC(1/4)
SbfI          922  CCTGCAGG             CCTGCA^GG
SgrAI        2568  CACCGGCG             C[GA]^CCGG[CT]G
Sse8387I      922  CCTGCAGG             CCTGCA^GG
SspBI        1112  TGTACA               T^GTACA
Tru9I        1745  TTAA                 T^TAA
XhoI         1417  CTCGAG               C^TCGAG
XhoII         431  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI           28  GTATAC               GT^[AC][GT]AC
             2038  GTCGAC              
AflIII        293  ACGTGT               A^C[GA][CT]GT
             2354  ACACGT              
Asp700I       495  GAAACCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2094  GAAGAACTTC          
AspHI         360  GAGCTC               G[AT]GC[AT]^C
              523  GTGCTC              
BbsI         2020  GAAGAC               GAAGAC(2/6)
             1327  GTCTTC              
BbvCI         159  CCTCAGC              CCTCAGC(-5/-2)
             1179  GCTGAGG             
BpuAI        2020  GAAGAC               GAAGAC(2/6)
             1327  GTCTTC              
BsaAI         292  TACGTG               [CT]AC^GT[GA]
             1074  CACGTG              
BsaBI         510  GATTGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1941  GATGGGCATC          
BsiHKAI       360  GAGCTC               G[AT]GC[AT]^C
              523  GTGCTC              
BsrDI         537  CATTGC               GCAATG(2/0)
             2499  CATTGC              
BstAPI        609  GCAGATTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1253  GCAGCCCGTGC         
BstEII       1098  GGTCACC              G^GT[ACGT]ACC
             1394  GGTCACC             
BstPI        1098  GGTCACC              G^GT[ACGT]ACC
             1394  GGTCACC             
EarI          357  GAAGAG               CTCTTC(1/4)
              990  GAAGAG              
EcoO65I      1098  GGTCACC              G^GT[ACGT]ACC
             1394  GGTCACC             
HgaI         1292  GACGC                GACGC(5/10)
             1504  GCGTC               
Ksp632I       357  GAAGAG               CTCTTC(1/4)
              990  GAAGAG              
MamI          510  GATTGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1941  GATGGGCATC          
PvuII        1168  CAGCTG               CAG^CTG
             2454  CAGCTG              
StuI         1011  AGGCCT               AGG^CCT
             1967  AGGCCT              
TfiI          612  GATTC                G^A[AT]TC
             1034  GATTC               
Tsp509I       235  AATT                 ^AATT
             1743  AATT                
TspRI         699  CACTG                CA[GC]TG[ACGT][ACGT]^
             2131  CAGTG               
XmnI          495  GAAACCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2094  GAAGAACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  106 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        ApaI        AvaI        AvaII       BanI        
BanII       BbeI        BbvI        BcnI        BmyI        
BpmI        BsaHI       BsaJI       BsgI        BsiEI       
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrBI       BsrFI       BsrI        
BssKI       BssSI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EagI        
EclXI       Eco52I      EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KasI        MaeII       
MaeIII      MboI        MboII       MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NdeII       NgoMIV      
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       PstI        RsaI        Sau3AI      Sau96I      
ScaI        ScrFI       SfaNI       SfcI        SfoI        
SmaI        SmlI        StyI        TaqI        TseI        
Tsp45I      TthHB8I     Van91I      XcmI        XmaCI       
XmaI        
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