Restriction map for pF1KB7332
 (Linear) (Six-base) MAPSORT of: KIBB7332.seq  Check: 3480  from: 1  to: 3096



KIBB7332.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AccI GT'mk_AC

Cuts at:      0     680    3096 
   Size:        680    2416
 
Acc65I G'GTAC_C

Cuts at:      0    2481    3096 
   Size:       2481     615
 
AclI AA'CG_TT

Cuts at:      0    2919    3096 
   Size:       2919     177
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     224    1246    1780    1792    2777    3096 
   Size:        224    1022     534      12     985     319

  Fragments arranged by size: 

                1022     985     534     319     224      12
 
AflIII A'CryG_T

Cuts at:      0    2025    3096 
   Size:       2025    1071
 
AleI CACnn'nnGTG

Cuts at:      0    2932    3096 
   Size:       2932     164
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2618    3096 
   Size:       2618     478
 
ApaI G_GGCC'C

Cuts at:      0     139     645    3096 
   Size:        139     506    2451

  Fragments arranged by size: 

                2451     506     139
 
ApoI r'AATT_y

Cuts at:      0     568     579     736    1447    1783    2487    2694    3096 
   Size:        568      11     157     711     336     704     207     402

  Fragments arranged by size: 

                 711     704     568     402     336     207     157      11
            
 
AvaI C'yCGr_G

Cuts at:      0     253    1038    3096 
   Size:        253     785    2058

  Fragments arranged by size: 

                2058     785     253
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2211    2244    3096 
   Size:       2211      33     852

  Fragments arranged by size: 

                2211     852      33
 
BamHI G'GATC_C

Cuts at:      0     415    3096 
   Size:        415    2681
 
BanI G'GyrC_C

Cuts at:      0    2481    3096 
   Size:       2481     615
 
BanII G_rGCy'C

Cuts at:      0     139     645     816    1170    2200    3096 
   Size:        139     506     171     354    1030     896

  Fragments arranged by size: 

                1030     896     506     354     171     139
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1969    3096 
   Size:       1969    1127
 
BclI T'GATC_A

Cuts at:      0     245    3096 
   Size:        245    2851
 
BfrBI ATG'CAT

Cuts at:      0    2008    3096 
   Size:       2008    1088
 
BglII A'GATC_T

Cuts at:      0     963    1488    3096 
   Size:        963     525    1608

  Fragments arranged by size: 

                1608     963     525
 
Bme1580I G_kGCm'C

Cuts at:      0     139     506     645    1869    3096 
   Size:        139     367     139    1224    1227

  Fragments arranged by size: 

                1227    1224     367     139     139
 
BmgBI CAC'GTC

Cuts at:      0     299    1626    3096 
   Size:        299    1327    1470

  Fragments arranged by size: 

                1470    1327     299
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295    2018    2567    3096 
   Size:        295    1723     549     529

  Fragments arranged by size: 

                1723     549     529     295
 
Bpu10I CC'TnA_GC

Cuts at:      0    1381    3096 
   Size:       1381    1715
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2148    2785    3096 
   Size:       2148     637     311

  Fragments arranged by size: 

                2148     637     311
 
BsaWI w'CCGG_w

Cuts at:      0    2630    3096 
   Size:       2630     466
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     911     941    3096 
   Size:        911      30    2155

  Fragments arranged by size: 

                2155     911      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     806     824    3096 
   Size:        806      18    2272

  Fragments arranged by size: 

                2272     806      18
 
BseYI C'CCAG_C

Cuts at:      0     315    3096 
   Size:        315    2781
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157    1238    3096 
   Size:        157    1081    1858

  Fragments arranged by size: 

                1858    1081     157
 
BsiEI CG_ry'CG

Cuts at:      0      89      93     167    3096 
   Size:         89       4      74    2929

  Fragments arranged by size: 

                2929      89      74       4
 
BsiHKAI G_wGCw'C

Cuts at:      0     816     981     994    1170    2200    3096 
   Size:        816     165      13     176    1030     896

  Fragments arranged by size: 

                1030     896     816     176     165      13
 
BsmI GAATG_Cn'

Cuts at:      0    1059    2557    3096 
   Size:       1059    1498     539

  Fragments arranged by size: 

                1498    1059     539
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      46    3096 
   Size:         46    3050
 
Bsp1286I G_dGCh'C

Cuts at:      0     139     506     645     816     981     994    1170    1869 
   Size:        139     367     139     171     165      13     176     699

Cuts at:   1869    2200    3096 
   Size:        331     896

  Fragments arranged by size: 

                 896     699     367     331     176     171     165     139
                 139      13
 
BsrBI CCG'CTC

Cuts at:      0    2212    3096 
   Size:       2212     884
 
BsrDI GCAATG_nn'

Cuts at:      0    1064    2612    3093    3096 
   Size:       1064    1548     481       3

  Fragments arranged by size: 

                1548    1064     481       3
 
BsrFI r'CCGG_y

Cuts at:      0      88    2762    3096 
   Size:         88    2674     334

  Fragments arranged by size: 

                2674     334      88
 
BsrGI T'GTAC_A

Cuts at:      0    1022    3096 
   Size:       1022    2074
 
BssHII G'CGCG_C

Cuts at:      0     119    3096 
   Size:        119    2977
 
BssSI C'ACGA_G

Cuts at:      0     970    3096 
   Size:        970    2126
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1351    3096 
   Size:       1351    1745
 
BstYI r'GATC_y

Cuts at:      0     415     963    1226    1488    3096 
   Size:        415     548     263     262    1608

  Fragments arranged by size: 

                1608     548     415     263     262
 
BtgI C'CryG_G

Cuts at:      0     180    2039    3096 
   Size:        180    1859    1057

  Fragments arranged by size: 

                1859    1057     180
 
BtsI GCAGTG_nn'

Cuts at:      0     215    3096 
   Size:        215    2881
 
DraI TTT'AAA

Cuts at:      0    2309    2693    3015    3096 
   Size:       2309     384     322      81

  Fragments arranged by size: 

                2309     384     322      81
 
EaeI y'GGCC_r

Cuts at:      0      90     291    1212    2764    3096 
   Size:         90     201     921    1552     332

  Fragments arranged by size: 

                1552     921     332     201      90
 
EagI C'GGCC_G

Cuts at:      0      90    3096 
   Size:         90    3006
 
EarI CTCTTCn'nnn_

Cuts at:      0     353    1005    1611    2069    2210    2252    3048    3096 
   Size:        353     652     606     458     141      42     796      48

  Fragments arranged by size: 

                 796     652     606     458     353     141      48      42
            
 
EcoICRI GAG'CTC

Cuts at:      0     814    1168    2198    3096 
   Size:        814     354    1030     898

  Fragments arranged by size: 

                1030     898     814     354
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     224     295    1246    1780    1792    2018    2567    2777 
   Size:        224      71     951     534      12     226     549     210

Cuts at:   2777    3096 
   Size:        319

  Fragments arranged by size: 

                 951     549     534     319     226     224     210      71
                  12
 
EcoO109I rG'GnC_Cy

Cuts at:      0      81     135     136     641     642    2220    3096 
   Size:         81      54       1     505       1    1578     876

  Fragments arranged by size: 

                1578     876     505      81      54       1       1
 
EcoRI G'AATT_C

Cuts at:      0    1783    3096 
   Size:       1783    1313
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    3024    3096 
   Size:       3024      72
 
FseI GG_CCGG'CC

Cuts at:      0    2766    3096 
   Size:       2766     330
 
FspI TGC'GCA

Cuts at:      0    2879    3096 
   Size:       2879     217
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     260     292     354     386     654     686     746     778 
   Size:        260      32      62      32     268      32      60      32

Cuts at:    778     911     943     975    1213    1245    1250    1282    2146 
   Size:        133      32      32     238      32       5      32     864

Cuts at:   2146    2178    2182    2214    3096 
   Size:         32       4      32     882

  Fragments arranged by size: 

                 882     864     268     260     238     133      62      60
                  32      32      32      32      32      32      32      32
                  32      32       5       4
 
HincII GTy'rAC

Cuts at:      0    1235    1407    3096 
   Size:       1235     172    1689

  Fragments arranged by size: 

                1689    1235     172
 
HindIII A'AGCT_T

Cuts at:      0     901    3096 
   Size:        901    2195
 
KpnI G_GTAC'C

Cuts at:      0    2485    3096 
   Size:       2485     611
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     744     786    1056    2775    3043    3096 
   Size:        744      42     270    1719     268      53

  Fragments arranged by size: 

                1719     744     270     268      53      42
 
MslI CAynn'nnrTG

Cuts at:      0    1645    2932    3096 
   Size:       1645    1287     164

  Fragments arranged by size: 

                1645    1287     164
 
MspA1I CmG'CkG

Cuts at:      0     315    3096 
   Size:        315    2781
 
NaeI GCC'GGC

Cuts at:      0    2764    3096 
   Size:       2764     332
 
NdeI CA'TA_TG

Cuts at:      0    2010    2988    3096 
   Size:       2010     978     108

  Fragments arranged by size: 

                2010     978     108
 
NgoMIV G'CCGG_C

Cuts at:      0    2762    3096 
   Size:       2762     334
 
NsiI A_TGCA'T

Cuts at:      0    2010    3096 
   Size:       2010    1086
 
NspI r_CATG'y

Cuts at:      0    2029    2242    2884    3096 
   Size:       2029     213     642     212

  Fragments arranged by size: 

                2029     642     213     212
 
PciI A'CATG_T

Cuts at:      0    2025    3096 
   Size:       2025    1071
 
PfoI T'CCnGG_A

Cuts at:      0      47    2186    3096 
   Size:         47    2139     910

  Fragments arranged by size: 

                2139     910      47
 
PpuMI rG'GwC_Cy

Cuts at:      0      81    2220    3096 
   Size:         81    2139     876

  Fragments arranged by size: 

                2139     876      81
 
PsiI TTA'TAA

Cuts at:      0    1550    3096 
   Size:       1550    1546
 
PspOMI G'GGCC_C

Cuts at:      0     135     641    3096 
   Size:        135     506    2455

  Fragments arranged by size: 

                2455     506     135
 
PstI C_TGCA'G

Cuts at:      0    2134    3096 
   Size:       2134     962
 
PvuII CAG'CTG

Cuts at:      0     315    3096 
   Size:        315    2781
 
SacI G_AGCT'C

Cuts at:      0     816    1170    2200    3096 
   Size:        816     354    1030     896

  Fragments arranged by size: 

                1030     896     816     354
 
SanDI GG'GwC_CC

Cuts at:      0      81    3096 
   Size:         81    3015
 
SfcI C'TryA_G

Cuts at:      0    1112    1394    1668    2130    2294    3096 
   Size:       1112     282     274     462     164     802

  Fragments arranged by size: 

                1112     802     462     282     274     164
 
SmaI CCC'GGG

Cuts at:      0     255    3096 
   Size:        255    2841
 
SmlI C'TyrA_G

Cuts at:      0    2163    2800    3096 
   Size:       2163     637     296

  Fragments arranged by size: 

                2163     637     296
 
SphI G_CATG'C

Cuts at:      0    2242    3096 
   Size:       2242     854
 
StyI C'CwwG_G

Cuts at:      0    1746    3096 
   Size:       1746    1350
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     153     196     996    1349    3096 
   Size:        153      43     800     353    1747

  Fragments arranged by size: 

                1747     800     353     153      43
 
TatI w'GTAC_w

Cuts at:      0    1022    1774    2663    2970    3096 
   Size:       1022     752     889     307     126

  Fragments arranged by size: 

                1022     889     752     307     126
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     407     439    3096 
   Size:        407      32    2657

  Fragments arranged by size: 

                2657     407      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2466    2542    2778    3096 
   Size:       2466      76     236     318

  Fragments arranged by size: 

                2466     318     236      76
 
XmaI C'CCGG_G

Cuts at:      0     253    3096 
   Size:        253    2843
 
XmnI GAAnn'nnTTC

Cuts at:      0    1259    3096 
   Size:       1259    1837
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AleI     AlfI     ApaI
     ApoI     AvaI     BaeI    BamHI     BanI    BanII     BbsI     BclI
    BfrBI    BglII Bme1580I    BmgBI     BpmI   Bpu10I    BpuEI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstYI     BtgI     BtsI     DraI     EaeI     EagI     EarI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI     FseI     FspI    Hin4I   HincII
  HindIII     KpnI     MmeI     MslI   MspA1I     NaeI     NdeI   NgoMIV
     NsiI     NspI     PciI     PfoI    PpuMI     PsiI   PspOMI     PstI
    PvuII     SacI    SanDI     SfcI     SmaI     SmlI     SphI     StyI
    TaqII     TatI     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AhdI     AloI    AlwNI
    ApaLI     AscI     AseI    AsiSI    AvrII     BbeI    BbvCI     BcgI
    BciVI     BglI     BlpI     BmrI     BmtI     BplI     BsaI    BsaAI
    BsaBI    BsaHI    BsiWI    BspEI    BspHI    BspMI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI   DraIII     DrdI
     EciI    EcoNI    EcoRV    FspAI    HaeII     HpaI     KasI     MfeI
     MluI     MscI     NarI     NcoI     NheI     NotI     NruI     PacI
     PasI    PflMI     PmeI     PmlI     PpiI    PshAI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI
     StuI     SwaI  Tth111I     XbaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com