Restriction map for pF1KB6543
 (Linear) (Six-base) MAPSORT of: KIBB6543.seq  Check: 8871  from: 1  to: 1107



KIBB6543.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     344     898    1107 
   Size:        344     554     209

  Fragments arranged by size: 

                 554     344     209
 
AflIII A'CryG_T

Cuts at:      0     656    1107 
   Size:        656     451
 
AlwNI CAG_nnn'CTG

Cuts at:      0     450    1107 
   Size:        450     657
 
ApaI G_GGCC'C

Cuts at:      0    1023    1077    1107 
   Size:       1023      54      30

  Fragments arranged by size: 

                1023      54      30
 
ApoI r'AATT_y

Cuts at:      0     866    1107 
   Size:        866     241
 
BanII G_rGCy'C

Cuts at:      0     239     468    1023    1077    1107 
   Size:        239     229     555      54      30

  Fragments arranged by size: 

                 555     239     229      54      30
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     107    1107 
   Size:        107    1000
 
BlpI GC'TnA_GC

Cuts at:      0     701    1107 
   Size:        701     406
 
Bme1580I G_kGCm'C

Cuts at:      0    1023    1077    1107 
   Size:       1023      54      30

  Fragments arranged by size: 

                1023      54      30
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     585     744    1080    1107 
   Size:        585     159     336      27

  Fragments arranged by size: 

                 585     336     159      27
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     379     452     948    1107 
   Size:        379      73     496     159

  Fragments arranged by size: 

                 496     379     159      73
 
BsaHI Gr'CG_yC

Cuts at:      0      85    1107 
   Size:         85    1022
 
BsaWI w'CCGG_w

Cuts at:      0     566    1107 
   Size:        566     541
 
BseYI C'CCAG_C

Cuts at:      0      54     239     503    1107 
   Size:         54     185     264     604

  Fragments arranged by size: 

                 604     264     185      54
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     324     580    1107 
   Size:        324     256     527

  Fragments arranged by size: 

                 527     324     256
 
BsiHKAI G_wGCw'C

Cuts at:      0     554     702    1107 
   Size:        554     148     405

  Fragments arranged by size: 

                 554     405     148
 
Bsp1286I G_dGCh'C

Cuts at:      0     239     468     554     702    1023    1077    1107 
   Size:        239     229      86     148     321      54      30

  Fragments arranged by size: 

                 321     239     229     148      86      54      30
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     558    1107 
   Size:        558     549
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     734    1107 
   Size:        734     373
 
BstYI r'GATC_y

Cuts at:      0     362     891    1107 
   Size:        362     529     216

  Fragments arranged by size: 

                 529     362     216
 
BtgI C'CryG_G

Cuts at:      0     403     785     806     909    1107 
   Size:        403     382      21     103     198

  Fragments arranged by size: 

                 403     382     198     103      21
 
DraIII CAC_nnn'GTG

Cuts at:      0     558     691    1107 
   Size:        558     133     416

  Fragments arranged by size: 

                 558     416     133
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     344     379     452     898     948    1107 
   Size:        344      35      73     446      50     159

  Fragments arranged by size: 

                 446     344     159      73      50      35
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     172    1027    1107 
   Size:        172     855      80

  Fragments arranged by size: 

                 855     172      80
 
EcoO109I rG'GnC_Cy

Cuts at:      0     399    1019    1020    1033    1040    1074    1107 
   Size:        399     620       1      13       7      34      33

  Fragments arranged by size: 

                 620     399      34      33      13       7       1
 
EcoRI G'AATT_C

Cuts at:      0     866    1107 
   Size:        866     241
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     642    1107 
   Size:        642     465
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     803     835     974    1006    1107 
   Size:        803      32     139      32     101

  Fragments arranged by size: 

                 803     139     101      32      32
 
MslI CAynn'nnrTG

Cuts at:      0     430     710    1107 
   Size:        430     280     397

  Fragments arranged by size: 

                 430     397     280
 
MspA1I CmG'CkG

Cuts at:      0      45     112     587    1107 
   Size:         45      67     475     520

  Fragments arranged by size: 

                 520     475      67      45
 
NcoI C'CATG_G

Cuts at:      0     806    1107 
   Size:        806     301
 
PasI CC'CwG_GG

Cuts at:      0     461    1107 
   Size:        461     646
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     546     691    1107 
   Size:        546     145     416

  Fragments arranged by size: 

                 546     416     145
 
PfoI T'CCnGG_A

Cuts at:      0     395    1107 
   Size:        395     712
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     214     246    1107 
   Size:        214      32     861

  Fragments arranged by size: 

                 861     214      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     399    1107 
   Size:        399     708
 
PspOMI G'GGCC_C

Cuts at:      0    1019    1073    1107 
   Size:       1019      54      34

  Fragments arranged by size: 

                1019      54      34
 
PstI C_TGCA'G

Cuts at:      0      44     446     965    1107 
   Size:         44     402     519     142

  Fragments arranged by size: 

                 519     402     142      44
 
PvuII CAG'CTG

Cuts at:      0      45     112     587    1107 
   Size:         45      67     475     520

  Fragments arranged by size: 

                 520     475      67      45
 
SfcI C'TryA_G

Cuts at:      0      40     442     961     972    1107 
   Size:         40     402     519      11     135

  Fragments arranged by size: 

                 519     402     135      40      11
 
StyI C'CwwG_G

Cuts at:      0     806    1023    1107 
   Size:        806     217      84

  Fragments arranged by size: 

                 806     217      84
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     740    1107 
   Size:        740     367
 
TatI w'GTAC_w

Cuts at:      0     203    1107 
   Size:        203     904
 
XmnI GAAnn'nnTTC

Cuts at:      0     763    1107 
   Size:        763     344
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     ApaI     ApoI    BanII     BbsI     BlpI
 Bme1580I     BmrI     BpmI    BsaHI    BsaWI    BseYI     BsgI  BsiHKAI
 Bsp1286I   BstAPI    BstXI    BstYI     BtgI   DraIII  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI    Hin4I     MslI   MspA1I     NcoI     PasI
    PflMI     PfoI     PpiI    PpuMI   PspOMI     PstI    PvuII     SfcI
     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII    BmgBI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaXI    BseRI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoRV     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI     PmeI     PmlI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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