Restriction map for pF1KB6756
 (Circular) (Six-base) MAPSORT of: pF1KB6756.seq  Check: 6013  from: 1  to: 3551



pF1KB6756  3551 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AccI GT'mk_AC

Cuts at:    589     589 
   Size:       3551
 
Acc65I G'GTAC_C

Cuts at:    567     567 
   Size:       3551
 
AclI AA'CG_TT

Cuts at:    545    3391     545 
   Size:       2846     705
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1376    1808    2555    2823    1376 
   Size:        432     747     268    2104

  Fragments arranged by size: 

                2104     747     432     268
 
AflIII A'CryG_T

Cuts at:    365     903    2007    2984     365 
   Size:        538    1104     977     932

  Fragments arranged by size: 

                1104     977     932     538
 
AgeI A'CCGG_T

Cuts at:   1939    1939 
   Size:       3551
 
AlwNI CAG_nnn'CTG

Cuts at:    214     856    2423     214 
   Size:        642    1567    1342

  Fragments arranged by size: 

                1567    1342     642
 
ApaLI G'TGCA_C

Cuts at:   2321    2321 
   Size:       3551
 
ApoI r'AATT_y

Cuts at:    555    2904    3160     555 
   Size:       2349     256     946

  Fragments arranged by size: 

                2349     946     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3551
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3551
 
AvaI C'yCGr_G

Cuts at:    571     571 
   Size:       3551
 
BamHI G'GATC_C

Cuts at:    576     576 
   Size:       3551
 
BanI G'GyrC_C

Cuts at:    567     981    1229    1264     567 
   Size:        414     248      35    2854

  Fragments arranged by size: 

                2854     414     248      35
 
BanII G_rGCy'C

Cuts at:    245     565    1595     245 
   Size:        320    1030    2201

  Fragments arranged by size: 

                2201    1030     320
 
BbeI G_GCGC'C

Cuts at:    985    1233     985 
   Size:        248    3303
 
BbsI GAAGACnn'nnnn_

Cuts at:    397     397 
   Size:       3551
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124     366     400      90 
   Size:         34     242      34    3241

  Fragments arranged by size: 

                3241     242      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   1449    2210    3551    1449 
   Size:        761    1341    1449

  Fragments arranged by size: 

                1449    1341     761
 
BclI T'GATC_A

Cuts at:    359    1071     359 
   Size:        712    2839
 
BfrBI ATG'CAT

Cuts at:    111     111 
   Size:       3551
 
BglI GCCn_nnn'nGGC

Cuts at:    194     194 
   Size:       3551
 
BglII A'GATC_T

Cuts at:   1066    3103    1066 
   Size:       2037    1514
 
BlpI GC'TnA_GC

Cuts at:    406     663     406 
   Size:        257    3294
 
Bme1580I G_kGCm'C

Cuts at:   1176    1269    2325    1176 
   Size:         93    1056    2402

  Fragments arranged by size: 

                2402    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    151     480     763     844    1179    3323     151 
   Size:        329     283      81     335    2144     379

  Fragments arranged by size: 

                2144     379     335     329     283      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1918    1918 
   Size:       3551
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    727    1606    2098    2396     727 
   Size:        879     492     298    1882

  Fragments arranged by size: 

                1882     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    435    3255     435 
   Size:       2820     731
 
BsaAI yAC'GTr

Cuts at:    904     904 
   Size:       3551
 
BsaBI GATnn'nnATC

Cuts at:      6    1065    3102       6 
   Size:       1059    2037     455

  Fragments arranged by size: 

                2037    1059     455
 
BsaHI Gr'CG_yC

Cuts at:    982    1230    1932    3428     982 
   Size:        248     702    1496    1105

  Fragments arranged by size: 

                1496    1105     702     248
 
BsaWI w'CCGG_w

Cuts at:    740    1261    1939    2213    2360    2771     740 
   Size:        521     678     274     147     411    1520

  Fragments arranged by size: 

                1520     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    109     265     109 
   Size:        156    3395
 
BseYI C'CCAG_C

Cuts at:    436     976    1734    2311    2807     436 
   Size:        540     758     577     496    1180

  Fragments arranged by size: 

                1180     758     577     540     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    362     362 
   Size:       3551
 
BsiEI CG_ry'CG

Cuts at:    104    1093    1139    2347     104 
   Size:        989      46    1208    1308

  Fragments arranged by size: 

                1308    1208     989      46
 
BsiHKAI G_wGCw'C

Cuts at:    245     565    1343    1533    2325     245 
   Size:        320     778     190     792    1471

  Fragments arranged by size: 

                1471     792     778     320     190
 
Bsp1286I G_dGCh'C

Cuts at:    245     565    1176    1269    1343    1533    1595    2325     245 
   Size:        320     611      93      74     190      62     730    1471

  Fragments arranged by size: 

                1471     730     611     320     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2771    2771 
   Size:       3551
 
BspHI T'CATG_A

Cuts at:   2727    2727 
   Size:       3551
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    509     601     815    1117    1498     509 
   Size:         92     214     302     381    2562

  Fragments arranged by size: 

                2562     381     302     214      92
 
BsrBI CCG'CTC

Cuts at:     50     117     288     410    1843    1897    3381    3546      50 
   Size:         67     171     122    1433      54    1484     165      55

  Fragments arranged by size: 

                1484    1433     171     165     122      67      55      54
            
 
BsrDI GCAATG_nn'

Cuts at:    173     300    1463     173 
   Size:        127    1163    2261

  Fragments arranged by size: 

                2261    1163     127
 
BsrFI r'CCGG_y

Cuts at:   1549    1730    1939    1549 
   Size:        181     209    3161

  Fragments arranged by size: 

                3161     209     181
 
BssSI C'ACGA_G

Cuts at:   1822    2180    1822 
   Size:        358    3193
 
BstAPI GCAn_nnn'nTGC

Cuts at:    928     928 
   Size:       3551
 
BstBI TT'CG_AA

Cuts at:   1912    1912 
   Size:       3551
 
BstYI r'GATC_y

Cuts at:      1     576    1043    1066    1401    1647    2659    2745    2757 
   Size:        575     467      23     335     246    1012      86      12

Cuts at:   2757    3103       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     575     467     449     346     335     246      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:    235     235 
   Size:       3551
 
BtgI C'CryG_G

Cuts at:    167     167 
   Size:       3551
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1501    1682    1845    1501 
   Size:        181     163    3207

  Fragments arranged by size: 

                3207     181     163
 
BtsI GCAGTG_nn'

Cuts at:    384     794     384 
   Size:        410    3141
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    156     191     156 
   Size:         35    3516
 
DraI TTT'AAA

Cuts at:    515     550     515 
   Size:         35    3516
 
DrdI GACnn_nn'nnGTC

Cuts at:   1257    2115    1257 
   Size:        858    2693
 
EaeI y'GGCC_r

Cuts at:   1136    1310    1701    1728    3073    1136 
   Size:        174     391      27    1345    1614

  Fragments arranged by size: 

                1614    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1136    1136 
   Size:       3551
 
EarI CTCTTCn'nnn_

Cuts at:   1574    1784    1574 
   Size:        210    3341
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1759    2069    2215    3361    1759 
   Size:        310     146    1146    1949

  Fragments arranged by size: 

                1949    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    243     563     243 
   Size:        320    3231
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1376    1808    2555    2823    1376 
   Size:        432     747     268    2104

  Fragments arranged by size: 

                2104     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    211     498     690     211 
   Size:        287     192    3072

  Fragments arranged by size: 

                3072     287     192
 
EcoRI G'AATT_C

Cuts at:    555     555 
   Size:       3551
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1570    1570 
   Size:       3551
 
FspI TGC'GCA

Cuts at:   1332    1332 
   Size:       3551
 
HaeII r_GCGC'y

Cuts at:    157     985    1233    2255    3234     157 
   Size:        828     248    1022     979     474

  Fragments arranged by size: 

                1022     979     828     474     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1393    1423    1425    1455    1393 
   Size:         30       2      30    3489

  Fragments arranged by size: 

                3489      30      30       2
 
HincII GTy'rAC

Cuts at:    164     261     590     164 
   Size:         97     329    3125

  Fragments arranged by size: 

                3125     329      97
 
KasI G'GCGC_C

Cuts at:    981    1229     981 
   Size:        248    3303
 
KpnI G_GTAC'C

Cuts at:    571     571 
   Size:       3551
 
MluI A'CGCG_T

Cuts at:   2984    2984 
   Size:       3551
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2222    2406    2222 
   Size:        184    3367
 
MscI TGG'CCA

Cuts at:   1312    1312 
   Size:       3551
 
MslI CAynn'nnrTG

Cuts at:   1540    1667    1540 
   Size:        127    3424
 
MspA1I CmG'CkG

Cuts at:    328     436     662     976    1336    2349    2594     328 
   Size:        108     226     314     360    1013     245    1285

  Fragments arranged by size: 

                1285    1013     360     314     245     226     108
 
NaeI GCC'GGC

Cuts at:   1732    1732 
   Size:       3551
 
NarI GG'CG_CC

Cuts at:    982    1230     982 
   Size:        248    3303
 
NgoMIV G'CCGG_C

Cuts at:   1730    1730 
   Size:       3551
 
NruI TCG'CGA

Cuts at:    530     530 
   Size:       3551
 
NsiI A_TGCA'T

Cuts at:    113     113 
   Size:       3551
 
NspI r_CATG'y

Cuts at:    336     460     604    1104    2011     336 
   Size:        124     144     500     907    1876

  Fragments arranged by size: 

                1876     907     500     144     124
 
PciI A'CATG_T

Cuts at:   2007    2007 
   Size:       3551
 
PmeI GTTT'AAAC

Cuts at:    550     550 
   Size:       3551
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    523     555    2690    2722     523 
   Size:         32    2135      32    1352

  Fragments arranged by size: 

                2135    1352      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    498     498 
   Size:       3551
 
PshAI GACnn'nnGTC

Cuts at:    169     169 
   Size:       3551
 
PstI C_TGCA'G

Cuts at:    379     598    1283     379 
   Size:        219     685    2647

  Fragments arranged by size: 

                2647     685     219
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3551
 
PvuII CAG'CTG

Cuts at:    328     436     976    1336     328 
   Size:        108     540     360    2543

  Fragments arranged by size: 

                2543     540     360     108
 
RsrII CG'GwC_CG

Cuts at:   1746    1746 
   Size:       3551
 
SacI G_AGCT'C

Cuts at:    245     565     245 
   Size:        320    3231
 
SalI G'TCGA_C

Cuts at:    588     588 
   Size:       3551
 
SapI GCTCTTCn'nnn_

Cuts at:   1574    1784    1574 
   Size:        210    3341
 
SbfI CC_TGCA'GG

Cuts at:    598     598 
   Size:       3551
 
ScaI AGT'ACT

Cuts at:   3068    3068 
   Size:       3551
 
SfcI C'TryA_G

Cuts at:     33     227     375     594    1279    2272    2463      33 
   Size:        194     148     219     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     219     194     191     148
 
SfoI GGC'GCC

Cuts at:    983    1231     983 
   Size:        248    3303
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3551
 
SmaI CCC'GGG

Cuts at:    573     573 
   Size:       3551
 
SmlI C'TyrA_G

Cuts at:    706    1621    2113    2375     706 
   Size:        915     492     262    1882

  Fragments arranged by size: 

                1882     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3551
 
SphI G_CATG'C

Cuts at:    604    1104     604 
   Size:        500    3051
 
StyI C'CwwG_G

Cuts at:    189     685    1039     189 
   Size:        496     354    2701

  Fragments arranged by size: 

                2701     496     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    283    1134    1267    1726    1935     283 
   Size:        851     133     459     209    1899

  Fragments arranged by size: 

                1899     851     459     209     133
 
TatI w'GTAC_w

Cuts at:    335    3066     335 
   Size:       2731     820
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3068    3100    3068 
   Size:         32    3519
 
Tth111I GACn'n_nGTC

Cuts at:   1348    1348 
   Size:       3551
 
XbaI T'CTAG_A

Cuts at:    582     582 
   Size:       3551
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    477    1046     477 
   Size:        569    2982
 
XmaI C'CCGG_G

Cuts at:    571     571 
   Size:       3551
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NruI
     NsiI     NspI     PciI     PmeI     PpiI    PpuMI    PshAI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI    BmgBI     BmtI     BpmI
   Bpu10I    BsaXI    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII
    BstXI  BstZ17I     ClaI   DraIII    EcoNI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NcoI     NdeI     NheI     NotI     PacI
     PasI    PflMI     PfoI     PmlI     PsiI   PspOMI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     XmnI     ZraI
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