Restriction map for pF1KB6756
 (Linear) (Six-base) MAPSORT of: KIBB6756.seq  Check: 5266  from: 1  to: 438



KIBB6756.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AflIII A'CryG_T

Cuts at:      0     257     438 
   Size:        257     181
 
AlwNI CAG_nnn'CTG

Cuts at:      0     106     438 
   Size:        106     332
 
BanII G_rGCy'C

Cuts at:      0     137     438 
   Size:        137     301
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     289     438 
   Size:        289     149
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     258     292     438 
   Size:        258      34     146

  Fragments arranged by size: 

                 258     146      34
 
BclI T'GATC_A

Cuts at:      0     251     438 
   Size:        251     187
 
BfrBI ATG'CAT

Cuts at:      0       3     438 
   Size:          3     435
 
BglI GCCn_nnn'nGGC

Cuts at:      0      86     438 
   Size:         86     352
 
BlpI GC'TnA_GC

Cuts at:      0     298     438 
   Size:        298     140
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      43     372     438 
   Size:         43     329      66

  Fragments arranged by size: 

                 329      66      43
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     327     438 
   Size:        327     111
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       1     157     438 
   Size:          1     156     281

  Fragments arranged by size: 

                 281     156       1
 
BseYI C'CCAG_C

Cuts at:      0     328     438 
   Size:        328     110
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     438 
   Size:        254     184
 
BsiHKAI G_wGCw'C

Cuts at:      0     137     438 
   Size:        137     301
 
Bsp1286I G_dGCh'C

Cuts at:      0     137     438 
   Size:        137     301
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     401     438 
   Size:        401      37
 
BsrBI CCG'CTC

Cuts at:      0       9     180     302     438 
   Size:          9     171     122     136

  Fragments arranged by size: 

                 171     136     122       9
 
BsrDI GCAATG_nn'

Cuts at:      0      65     192     438 
   Size:         65     127     246

  Fragments arranged by size: 

                 246     127      65
 
Bsu36I CC'TnA_GG

Cuts at:      0     127     438 
   Size:        127     311
 
BtgI C'CryG_G

Cuts at:      0      59     438 
   Size:         59     379
 
BtsI GCAGTG_nn'

Cuts at:      0     276     438 
   Size:        276     162
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      48      83     438 
   Size:         48      35     355

  Fragments arranged by size: 

                 355      48      35
 
DraI TTT'AAA

Cuts at:      0     407     438 
   Size:        407      31
 
EcoICRI GAG'CTC

Cuts at:      0     135     438 
   Size:        135     303
 
EcoO109I rG'GnC_Cy

Cuts at:      0     103     390     438 
   Size:        103     287      48

  Fragments arranged by size: 

                 287     103      48
 
HaeII r_GCGC'y

Cuts at:      0      49     438 
   Size:         49     389
 
HincII GTy'rAC

Cuts at:      0      56     153     438 
   Size:         56      97     285

  Fragments arranged by size: 

                 285      97      56
 
MspA1I CmG'CkG

Cuts at:      0     220     328     438 
   Size:        220     108     110

  Fragments arranged by size: 

                 220     110     108
 
NruI TCG'CGA

Cuts at:      0     422     438 
   Size:        422      16
 
NsiI A_TGCA'T

Cuts at:      0       5     438 
   Size:          5     433
 
NspI r_CATG'y

Cuts at:      0     228     352     438 
   Size:        228     124      86

  Fragments arranged by size: 

                 228     124      86
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     415     438 
   Size:        415      23
 
PpuMI rG'GwC_Cy

Cuts at:      0     390     438 
   Size:        390      48
 
PshAI GACnn'nnGTC

Cuts at:      0      61     438 
   Size:         61     377
 
PstI C_TGCA'G

Cuts at:      0     271     438 
   Size:        271     167
 
PvuII CAG'CTG

Cuts at:      0     220     328     438 
   Size:        220     108     110

  Fragments arranged by size: 

                 220     110     108
 
SacI G_AGCT'C

Cuts at:      0     137     438 
   Size:        137     301
 
SfcI C'TryA_G

Cuts at:      0     119     267     438 
   Size:        119     148     171

  Fragments arranged by size: 

                 171     148     119
 
StyI C'CwwG_G

Cuts at:      0      81     438 
   Size:         81     357
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     175     438 
   Size:        175     263
 
TatI w'GTAC_w

Cuts at:      0     227     438 
   Size:        227     211
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     369     438 
   Size:        369      69
 

 Enzymes that do cut:

   AflIII    AlwNI    BanII     BbsI     BcgI     BclI    BfrBI     BglI
     BlpI     BmrI     BsaI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspMI    BsrBI    BsrDI   Bsu36I     BtgI     BtsI    CspCI     DraI
  EcoICRI EcoO109I    HaeII   HincII   MspA1I     NruI     NsiI     NspI
     PpiI    PpuMI    PshAI     PstI    PvuII     SacI     SfcI     StyI
    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI    BciVI    BglII Bme1580I    BmgBI     BmtI     BplI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
    BtgZI     ClaI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com