Restriction map for pF1KB5952
##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

AccIII      AclI        AcsI        AflII       AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AviII       BbeI        
BbrPI       BciVI       BclI        BfrI        BglII       
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BsiWI       BspDI       BspEI       BspLU11I    BspMI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    EarI        Eco47III    
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HindIII     HpaI        
KasI        Ksp632I     MamI        MfeI        MluI        
MroI        MunI        NarI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        SwaI        
TfiI        Tth111I     XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AatII         198  GACGTC               GACGT^C
Acc65I         12  GGTACC               G^GTACC
AccI          760  GTCTAC               GT^[AC][GT]AC
AcyI          198  GACGTC               G[GA]^CG[CT]C
AflIII       1254  ACACGT               A^C[GA][CT]GT
Asp718I        12  GGTACC               G^GTACC
BcgI         1210  CGAGCCACATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI          480  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      480  GCTGAGC              GC^T[ACGT]AGC
BsaHI         198  GACGTC               G[GA]^CG[CT]C
BsgI          720  CTGCAC               GTGCAG(16/14)
BsiEI        1049  CGGCCG               CG[GA][CT]^CG
BsmI          789  GAATGC               GAATGC(1/-1)
Bsp1407I      338  TGTACA               T^GTACA
BspHI         800  TCATGA               T^CATGA
BsrBI        1545  GAGCGG               CCGCTC(-3/-3)
BsrGI         338  TGTACA               T^GTACA
CelII         480  GCTGAGC              GC^T[ACGT]AGC
EagI         1049  CGGCCG               C^GGCCG
EclXI        1049  CGGCCG               C^GGCCG
Eco52I       1049  CGGCCG               C^GGCCG
EcoNI         204  CCTATCAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII         768  GGCGCT               [GA]GCGC^[CT]
Hin1I         198  GACGTC               G[GA]^CG[CT]C
HincII       1354  GTCAAC               GT[CT]^[GA]AC
HindII       1354  GTCAAC               GT[CT]^[GA]AC
KpnI           12  GGTACC               GGTAC^C
KspI         1052  CCGCGG               CCGC^GG
MseI         1516  TTAA                 T^TAA
NaeI          485  GCCGGC               GCC^GGC
NcoI            8  CCATGG               C^CATGG
NgoMIV        485  GCCGGC               G^CCGGC
PflMI         594  CCAGGGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII        1177  CAGCTG               CAG^CTG
RcaI          800  TCATGA               T^CATGA
SacI         1446  GAGCTC               GAGCT^C
SacII        1052  CCGCGG               CCGC^GG
ScaI          160  AGTACT               AGT^ACT
SfaNI         783  GATGC                GCATC(5/9)
SspBI         338  TGTACA               T^GTACA
Tru9I        1516  TTAA                 T^TAA
Tsp509I      1203  AATT                 ^AATT
Van91I        594  CCAGGGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AvrII         891  CCTAGG               C^CTAGG
              942  CCTAGG              
BamHI         278  GGATCC               G^GATCC
             1340  GGATCC              
BbsI         1465  GTCTTC               GAAGAC(2/6)
             1525  GTCTTC              
BbvCI         620  CCTCAGC              CCTCAGC(-5/-2)
              960  CCTCAGC             
BlnI          891  CCTAGG               C^CTAGG
              942  CCTAGG              
BpmI         1317  CTCCAG               CTGGAG(16/14)
              134  CTGGAG              
BpuAI        1465  GTCTTC               GAAGAC(2/6)
             1525  GTCTTC              
BsrDI         299  GCAATG               GCAATG(2/0)
             1612  GCAATG              
BssHII        844  GCGCGC               G^CGCGC
              846  GCGCGC              
BssSI        1400  CACGAG               CACGAG(-5/-1)
              673  CTCGTG              
BstF5I         17  CATCC                GGATG(2/0)
              426  GGATG               
BstXI         224  CCAGTGGCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              524  CCACTGTGCTGG        
BstYI         278  GGATCC               [GA]^GATC[CT]
             1340  GGATCC              
FokI           17  CATCC                GGATG(9/13)
              426  GGATG               
MflI          278  GGATCC               [GA]^GATC[CT]
             1340  GGATCC              
NspI          584  ACATGC               [GA]CATG^[CT]
             1216  ACATGC              
PaeR7I       1019  CTCGAG               C^TCGAG
             1135  CTCGAG              
PpuMI         139  GGGTCCC              [GA]G^G[AT]CC[CT]
              945  AGGACCC             
SmlI         1019  CTCGAG               C^T[CT][GA]AG
             1135  CTCGAG              
TaqI         1020  TCGA                 T^CGA
             1136  TCGA                
TthHB8I      1020  TCGA                 T^CGA
             1136  TCGA                
XhoI         1019  CTCGAG               C^TCGAG
             1135  CTCGAG              
XhoII         278  GGATCC               [GA]^GATC[CT]
             1340  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbvI        BcnI        BfaI        
BglI        BmyI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1286I    BsrFI       BsrI        BssKI       
BstNI       BstUI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfcI        
SmaI        StyI        TseI        Tsp45I      TspRI       
XcmI        XmaCI       XmaI        
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