Restriction map for pF1KB6308
 (Circular) (Six-base) MAPSORT of: pF1KB6308.seq  Check: 5069  from: 1  to: 3953



pF1KB6308  3953 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AccI GT'mk_AC

Cuts at:    991     991 
   Size:       3953
 
Acc65I G'GTAC_C

Cuts at:    969     969 
   Size:       3953
 
AclI AA'CG_TT

Cuts at:   3793    3793 
   Size:       3953
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    812     907    1778    2210    2957    3225     812 
   Size:         95     871     432     747     268    1540

  Fragments arranged by size: 

                1540     871     747     432     268      95
 
AflIII A'CryG_T

Cuts at:    914    1305    2409    3386     914 
   Size:        391    1104     977    1481

  Fragments arranged by size: 

                1481    1104     977     391
 
AgeI A'CCGG_T

Cuts at:    552    2341     552 
   Size:       1789    2164
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    542     574     542 
   Size:         32    3921
 
AlwNI CAG_nnn'CTG

Cuts at:    568    1258    2825     568 
   Size:        690    1567    1696

  Fragments arranged by size: 

                1696    1567     690
 
ApaLI G'TGCA_C

Cuts at:    796    2723     796 
   Size:       1927    2026
 
ApoI r'AATT_y

Cuts at:    304     678     957    3306    3562     304 
   Size:        374     279    2349     256     695

  Fragments arranged by size: 

                2349     695     374     279     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3953
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3953
 
AvaI C'yCGr_G

Cuts at:    374     973     374 
   Size:        599    3354
 
AvrII C'CTAG_G

Cuts at:    523     523 
   Size:       3953
 
BamHI G'GATC_C

Cuts at:    978     978 
   Size:       3953
 
BanI G'GyrC_C

Cuts at:    969    1383    1631    1666     969 
   Size:        414     248      35    3256

  Fragments arranged by size: 

                3256     414     248      35
 
BanII G_rGCy'C

Cuts at:    967    1997     967 
   Size:       1030    2923
 
BbeI G_GCGC'C

Cuts at:   1387    1635    1387 
   Size:        248    3705
 
BbsI GAAGACnn'nnnn_

Cuts at:    391     604     917     391 
   Size:        213     313    3427

  Fragments arranged by size: 

                3427     313     213
 
BciVI GTATCCnnnnn_n'

Cuts at:   1851    2612    3953    1851 
   Size:        761    1341    1851

  Fragments arranged by size: 

                1851    1341     761
 
BclI T'GATC_A

Cuts at:   1473    1473 
   Size:       3953
 
BglII A'GATC_T

Cuts at:    726    1468    3505     726 
   Size:        742    2037    1174

  Fragments arranged by size: 

                2037    1174     742
 
BlpI GC'TnA_GC

Cuts at:   1065    1065 
   Size:       3953
 
Bme1580I G_kGCm'C

Cuts at:    800    1578    1671    2727     800 
   Size:        778      93    1056    2026

  Fragments arranged by size: 

                2026    1056     778      93
 
BmgBI CAC'GTC

Cuts at:    915     915 
   Size:       3953
 
BmrI ACTGGGnnnn_n'

Cuts at:   1165    1246    1581    3725    1165 
   Size:         81     335    2144    1393

  Fragments arranged by size: 

                2144    1393     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    339    2320     339 
   Size:       1981    1972
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1129    2008    2500    2798    1129 
   Size:        879     492     298    2284

  Fragments arranged by size: 

                2284     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    456    3657     456 
   Size:       3201     752
 
BsaAI yAC'GTr

Cuts at:   1306    1306 
   Size:       3953
 
BsaBI GATnn'nnATC

Cuts at:      6    1467    3504       6 
   Size:       1461    2037     455

  Fragments arranged by size: 

                2037    1461     455
 
BsaHI Gr'CG_yC

Cuts at:   1384    1632    2334    3830    1384 
   Size:        248     702    1496    1507

  Fragments arranged by size: 

                1507    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:    552    1142    1663    2341    2615    2762    3173     552 
   Size:        590     521     678     274     147     411    1332

  Fragments arranged by size: 

                1332     678     590     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    272     272 
   Size:       3953
 
BseYI C'CCAG_C

Cuts at:   1378    2136    2713    3209    1378 
   Size:        758     577     496    2122

  Fragments arranged by size: 

                2122     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    180     180 
   Size:       3953
 
BsiEI CG_ry'CG

Cuts at:    104    1495    1541    2749     104 
   Size:       1391      46    1208    1308

  Fragments arranged by size: 

                1391    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    800     967    1745    1935    2727     800 
   Size:        167     778     190     792    2026

  Fragments arranged by size: 

                2026     792     778     190     167
 
BsmI GAATG_Cn'

Cuts at:    402     402 
   Size:       3953
 
Bsp1286I G_dGCh'C

Cuts at:    800     967    1578    1671    1745    1935    1997    2727     800 
   Size:        167     611      93      74     190      62     730    2026

  Fragments arranged by size: 

                2026     730     611     190     167      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   3173    3173 
   Size:       3953
 
BspHI T'CATG_A

Cuts at:    906    3129     906 
   Size:       2223    1730
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    202    1003    1217    1519    1900     202 
   Size:        801     214     302     381    2255

  Fragments arranged by size: 

                2255     801     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2245    2299    3783    3948      50 
   Size:       2195      54    1484     165      55

  Fragments arranged by size: 

                2195    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1865    1865 
   Size:       3953
 
BsrFI r'CCGG_y

Cuts at:    552    1951    2132    2341     552 
   Size:       1399     181     209    2164

  Fragments arranged by size: 

                2164    1399     209     181
 
BssSI C'ACGA_G

Cuts at:   2224    2582    2224 
   Size:        358    3595
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1330    1330 
   Size:       3953
 
BstBI TT'CG_AA

Cuts at:   2314    2314 
   Size:       3953
 
BstXI CCAn_nnnn'nTGG

Cuts at:    129     129 
   Size:       3953
 
BstYI r'GATC_y

Cuts at:      1     564     726     978    1445    1468    1803    2049    3061 
   Size:        563     162     252     467      23     335     246    1012

Cuts at:   3061    3147    3159    3505       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     563     467     449     346     335     252     246
                 162      86      23      12
 
BtgI C'CryG_G

Cuts at:    455     455 
   Size:       3953
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1903    2084    2247    1903 
   Size:        181     163    3609

  Fragments arranged by size: 

                3609     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1196    1196 
   Size:       3953
 
DraI TTT'AAA

Cuts at:    723     952     723 
   Size:        229    3724
 
DrdI GACnn_nn'nnGTC

Cuts at:   1659    2517    1659 
   Size:        858    3095
 
EaeI y'GGCC_r

Cuts at:   1538    1712    2103    2130    3475    1538 
   Size:        174     391      27    1345    2016

  Fragments arranged by size: 

                2016    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1538    1538 
   Size:       3953
 
EarI CTCTTCn'nnn_

Cuts at:    601    1976    2186     601 
   Size:       1375     210    2368

  Fragments arranged by size: 

                2368    1375     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2161    2471    2617    3763    2161 
   Size:        310     146    1146    2351

  Fragments arranged by size: 

                2351    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    965     965 
   Size:       3953
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    812     907    1778    2210    2957    3225     812 
   Size:         95     871     432     747     268    1540

  Fragments arranged by size: 

                1540     871     747     432     268      95
 
EcoO109I rG'GnC_Cy

Cuts at:    137    1092     137 
   Size:        955    2998
 
EcoRI G'AATT_C

Cuts at:    957     957 
   Size:       3953
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    243    1972     243 
   Size:       1729    2224
 
FspI TGC'GCA

Cuts at:   1734    1734 
   Size:       3953
 
HaeII r_GCGC'y

Cuts at:   1387    1635    2657    3636    1387 
   Size:        248    1022     979    1704

  Fragments arranged by size: 

                1704    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    343     375     451     483    1795    1825    1827    1857     343 
   Size:         32      76      32    1312      30       2      30    2439

  Fragments arranged by size: 

                2439    1312      76      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    992     992 
   Size:       3953
 
HindIII A'AGCT_T

Cuts at:    181     220     181 
   Size:         39    3914
 
KasI G'GCGC_C

Cuts at:   1383    1631    1383 
   Size:        248    3705
 
KpnI G_GTAC'C

Cuts at:    973     973 
   Size:       3953
 
MluI A'CGCG_T

Cuts at:   3386    3386 
   Size:       3953
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2624    2808    2624 
   Size:        184    3769
 
MscI TGG'CCA

Cuts at:   1714    1714 
   Size:       3953
 
MslI CAynn'nnrTG

Cuts at:    514    1942    2069     514 
   Size:       1428     127    2398

  Fragments arranged by size: 

                2398    1428     127
 
MspA1I CmG'CkG

Cuts at:   1064    1378    1738    2751    2996    1064 
   Size:        314     360    1013     245    2021

  Fragments arranged by size: 

                2021    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2134    2134 
   Size:       3953
 
NarI GG'CG_CC

Cuts at:   1384    1632    1384 
   Size:        248    3705
 
NgoMIV G'CCGG_C

Cuts at:   2132    2132 
   Size:       3953
 
NspI r_CATG'y

Cuts at:    270    1006    1506    2413     270 
   Size:        736     500     907    1810

  Fragments arranged by size: 

                1810     907     736     500
 
PciI A'CATG_T

Cuts at:   2409    2409 
   Size:       3953
 
PmeI GTTT'AAAC

Cuts at:    952     952 
   Size:       3953
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3092    3124    3092 
   Size:         32    3921
 
PstI C_TGCA'G

Cuts at:    330    1000    1685     330 
   Size:        670     685    2598

  Fragments arranged by size: 

                2598     685     670
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3953
 
PvuII CAG'CTG

Cuts at:   1378    1738    1378 
   Size:        360    3593
 
RsrII CG'GwC_CG

Cuts at:   2148    2148 
   Size:       3953
 
SacI G_AGCT'C

Cuts at:    967     967 
   Size:       3953
 
SalI G'TCGA_C

Cuts at:    990     990 
   Size:       3953
 
SapI GCTCTTCn'nnn_

Cuts at:   1976    2186    1976 
   Size:        210    3743
 
SbfI CC_TGCA'GG

Cuts at:   1000    1000 
   Size:       3953
 
ScaI AGT'ACT

Cuts at:   3470    3470 
   Size:       3953
 
SfcI C'TryA_G

Cuts at:     33     326     820     996    1681    2674    2865      33 
   Size:        293     494     176     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     494     293     191     176
 
SfoI GGC'GCC

Cuts at:   1385    1633    1385 
   Size:        248    3705
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3953
 
SmaI CCC'GGG

Cuts at:    975     975 
   Size:       3953
 
SmlI C'TyrA_G

Cuts at:    374    1108    2023    2515    2777     374 
   Size:        734     915     492     262    1550

  Fragments arranged by size: 

                1550     915     734     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3953
 
SphI G_CATG'C

Cuts at:   1006    1506    1006 
   Size:        500    3453
 
SspI AAT'ATT

Cuts at:    870     870 
   Size:       3953
 
StuI AGG'CCT

Cuts at:    859     859 
   Size:       3953
 
StyI C'CwwG_G

Cuts at:    523    1087    1441     523 
   Size:        564     354    3035

  Fragments arranged by size: 

                3035     564     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    775    1536    1669    2128    2337     775 
   Size:        761     133     459     209    2391

  Fragments arranged by size: 

                2391     761     459     209     133
 
TatI w'GTAC_w

Cuts at:   3468    3468 
   Size:       3953
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3470    3502    3470 
   Size:         32    3921
 
Tth111I GACn'n_nGTC

Cuts at:   1750    1750 
   Size:       3953
 
XbaI T'CTAG_A

Cuts at:    984     984 
   Size:       3953
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1448    1448 
   Size:       3953
 
XhoI C'TCGA_G

Cuts at:    374     374 
   Size:       3953
 
XmaI C'CCGG_G

Cuts at:    973     973 
   Size:       3953
 
XmnI GAAnn'nnTTC

Cuts at:    151     295     151 
   Size:        144    3809
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AloI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI
    BanII     BbeI     BbsI    BciVI     BclI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV
     NspI     PciI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     ApaI
     AscI     BaeI    BbvCI     BcgI    BfrBI     BglI     BmtI     BpmI
   Bpu10I    BsaXI    BsiWI    BsmBI    BsrGI   BssHII   BstEII  BstZ17I
   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI
     HpaI     MfeI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     SwaI     ZraI
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