Restriction map for pF1KE0510
 (Circular) (Six-base) MAPSORT of: pF1KE0510.seq  Check: 2555  from: 1  to: 3818



pF1KE0510  3818 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AccI GT'mk_AC

Cuts at:    856     856 
   Size:       3818
 
Acc65I G'GTAC_C

Cuts at:    386     834     386 
   Size:        448    3370
 
AclI AA'CG_TT

Cuts at:   3658    3658 
   Size:       3818
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    312     339     408    1643    2075    2822    3090     312 
   Size:         27      69    1235     432     747     268    1040

  Fragments arranged by size: 

                1235    1040     747     432     268      69      27
 
AfeI AGC'GCT

Cuts at:    296     296 
   Size:       3818
 
AflIII A'CryG_T

Cuts at:   1170    2274    3251    1170 
   Size:       1104     977    1737

  Fragments arranged by size: 

                1737    1104     977
 
AgeI A'CCGG_T

Cuts at:   2206    2206 
   Size:       3818
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    256     288     256 
   Size:         32    3786
 
AlwNI CAG_nnn'CTG

Cuts at:    396     631    1123    2690     396 
   Size:        235     492    1567    1524

  Fragments arranged by size: 

                1567    1524     492     235
 
ApaI G_GGCC'C

Cuts at:    256     256 
   Size:       3818
 
ApaLI G'TGCA_C

Cuts at:   2588    2588 
   Size:       3818
 
ApoI r'AATT_y

Cuts at:    688     746     822    3171    3427     688 
   Size:         58      76    2349     256    1079

  Fragments arranged by size: 

                2349    1079     256      76      58
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3818
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3818
 
AvaI C'yCGr_G

Cuts at:    256     317     838     256 
   Size:         61     521    3236

  Fragments arranged by size: 

                3236     521      61
 
BamHI G'GATC_C

Cuts at:    843     843 
   Size:       3818
 
BanI G'GyrC_C

Cuts at:    303     386     834    1248    1496    1531     303 
   Size:         83     448     414     248      35    2590

  Fragments arranged by size: 

                2590     448     414     248      83      35
 
BanII G_rGCy'C

Cuts at:    256     832    1862     256 
   Size:        576    1030    2212

  Fragments arranged by size: 

                2212    1030     576
 
BbeI G_GCGC'C

Cuts at:   1252    1500    1252 
   Size:        248    3570
 
BciVI GTATCCnnnnn_n'

Cuts at:   1716    2477    3818    1716 
   Size:        761    1341    1716

  Fragments arranged by size: 

                1716    1341     761
 
BclI T'GATC_A

Cuts at:   1338    1338 
   Size:       3818
 
BglI GCCn_nnn'nGGC

Cuts at:    251     251 
   Size:       3818
 
BglII A'GATC_T

Cuts at:   1333    3370    1333 
   Size:       2037    1781
 
BlpI GC'TnA_GC

Cuts at:    184     930     184 
   Size:        746    3072
 
Bme1580I G_kGCm'C

Cuts at:    256    1443    1536    2592     256 
   Size:       1187      93    1056    1482

  Fragments arranged by size: 

                1482    1187    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1030    1111    1446    3590    1030 
   Size:         81     335    2144    1258

  Fragments arranged by size: 

                2144    1258     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2185    2185 
   Size:       3818
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    361     361 
   Size:       3818
 
Bpu10I CC'TnA_GC

Cuts at:    511     511 
   Size:       3818
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    994    1873    2365    2663     994 
   Size:        879     492     298    2149

  Fragments arranged by size: 

                2149     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    171     440    3522     171 
   Size:        269    3082     467

  Fragments arranged by size: 

                3082     467     269
 
BsaAI yAC'GTr

Cuts at:   1171    1171 
   Size:       3818
 
BsaBI GATnn'nnATC

Cuts at:      6    1332    3369       6 
   Size:       1326    2037     455

  Fragments arranged by size: 

                2037    1326     455
 
BsaHI Gr'CG_yC

Cuts at:   1249    1497    2199    3695    1249 
   Size:        248     702    1496    1372

  Fragments arranged by size: 

                1496    1372     702     248
 
BsaWI w'CCGG_w

Cuts at:   1007    1528    2206    2480    2627    3038    1007 
   Size:        521     678     274     147     411    1787

  Fragments arranged by size: 

                1787     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    256     286     256 
   Size:         30    3788
 
BseYI C'CCAG_C

Cuts at:   1243    2001    2578    3074    1243 
   Size:        758     577     496    1987

  Fragments arranged by size: 

                1987     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1360    1406    2614     104 
   Size:       1256      46    1208    1308

  Fragments arranged by size: 

                1308    1256    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    832    1610    1800    2592     832 
   Size:        778     190     792    2058

  Fragments arranged by size: 

                2058     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    465     465 
   Size:       3818
 
Bsp1286I G_dGCh'C

Cuts at:    256     832    1443    1536    1610    1800    1862    2592     256 
   Size:        576     611      93      74     190      62     730    1482

  Fragments arranged by size: 

                1482     730     611     576     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   3038    3038 
   Size:       3818
 
BspHI T'CATG_A

Cuts at:   2994    2994 
   Size:       3818
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    868    1082    1384    1765     868 
   Size:        214     302     381    2921

  Fragments arranged by size: 

                2921     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2110    2164    3648    3813      50 
   Size:       2060      54    1484     165      55

  Fragments arranged by size: 

                2060    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1730    1730 
   Size:       3818
 
BsrFI r'CCGG_y

Cuts at:   1816    1997    2206    1816 
   Size:        181     209    3428

  Fragments arranged by size: 

                3428     209     181
 
BssSI C'ACGA_G

Cuts at:   2089    2447    2089 
   Size:        358    3460
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1195    1195 
   Size:       3818
 
BstBI TT'CG_AA

Cuts at:   2179    2179 
   Size:       3818
 
BstYI r'GATC_y

Cuts at:      1     843    1310    1333    1668    1914    2926    3012    3024 
   Size:        842     467      23     335     246    1012      86      12

Cuts at:   3024    3370       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     842     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    247     540     247 
   Size:        293    3525
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    206    1768    1949    2112     206 
   Size:       1562     181     163    1912

  Fragments arranged by size: 

                1912    1562     181     163
 
BtsI GCAGTG_nn'

Cuts at:    714    1061     714 
   Size:        347    3471
 
DraI TTT'AAA

Cuts at:    817     817 
   Size:       3818
 
DrdI GACnn_nn'nnGTC

Cuts at:   1524    2382    1524 
   Size:        858    2960
 
EaeI y'GGCC_r

Cuts at:    154    1403    1577    1968    1995    3340     154 
   Size:       1249     174     391      27    1345     632

  Fragments arranged by size: 

                1345    1249     632     391     174      27
 
EagI C'GGCC_G

Cuts at:   1403    1403 
   Size:       3818
 
EarI CTCTTCn'nnn_

Cuts at:   1841    2051    1841 
   Size:        210    3608
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2026    2336    2482    3628    2026 
   Size:        310     146    1146    2216

  Fragments arranged by size: 

                2216    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    830     830 
   Size:       3818
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    312     339     361     408    1643    2075    2822    3090     312 
   Size:         27      22      47    1235     432     747     268    1040

  Fragments arranged by size: 

                1235    1040     747     432     268      47      27      22
            
 
EcoO109I rG'GnC_Cy

Cuts at:    252     253     957     252 
   Size:          1     704    3113

  Fragments arranged by size: 

                3113     704       1
 
EcoRI G'AATT_C

Cuts at:    822     822 
   Size:       3818
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1837    1837 
   Size:       3818
 
FspI TGC'GCA

Cuts at:   1599    1599 
   Size:       3818
 
HaeII r_GCGC'y

Cuts at:    298    1252    1500    2522    3501     298 
   Size:        954     248    1022     979     615

  Fragments arranged by size: 

                1022     979     954     615     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    184     216     646     678    1660    1690    1692    1722     184 
   Size:         32     430      32     982      30       2      30    2280

  Fragments arranged by size: 

                2280     982     430      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    857     857 
   Size:       3818
 
KasI G'GCGC_C

Cuts at:   1248    1496    1248 
   Size:        248    3570
 
KpnI G_GTAC'C

Cuts at:    390     838     390 
   Size:        448    3370
 
MluI A'CGCG_T

Cuts at:   3251    3251 
   Size:       3818
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    211    2489    2673     211 
   Size:       2278     184    1356

  Fragments arranged by size: 

                2278    1356     184
 
MscI TGG'CCA

Cuts at:   1579    1579 
   Size:       3818
 
MslI CAynn'nnrTG

Cuts at:   1807    1934    1807 
   Size:        127    3691
 
MspA1I CmG'CkG

Cuts at:    227     929    1243    1603    2616    2861     227 
   Size:        702     314     360    1013     245    1184

  Fragments arranged by size: 

                1184    1013     702     360     314     245
 
NaeI GCC'GGC

Cuts at:   1999    1999 
   Size:       3818
 
NarI GG'CG_CC

Cuts at:   1249    1497    1249 
   Size:        248    3570
 
NcoI C'CATG_G

Cuts at:    247     540     247 
   Size:        293    3525
 
NgoMIV G'CCGG_C

Cuts at:   1997    1997 
   Size:       3818
 
NspI r_CATG'y

Cuts at:    871    1371    2278     871 
   Size:        500     907    2411

  Fragments arranged by size: 

                2411     907     500
 
PciI A'CATG_T

Cuts at:   2274    2274 
   Size:       3818
 
PmeI GTTT'AAAC

Cuts at:    817     817 
   Size:       3818
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    256     288    2957    2989     256 
   Size:         32    2669      32    1085

  Fragments arranged by size: 

                2669    1085      32      32
 
PspOMI G'GGCC_C

Cuts at:    252     252 
   Size:       3818
 
PstI C_TGCA'G

Cuts at:    302     401     865    1550     302 
   Size:         99     464     685    2570

  Fragments arranged by size: 

                2570     685     464      99
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3818
 
PvuII CAG'CTG

Cuts at:   1243    1603    1243 
   Size:        360    3458
 
RsrII CG'GwC_CG

Cuts at:   2013    2013 
   Size:       3818
 
SacI G_AGCT'C

Cuts at:    832     832 
   Size:       3818
 
SalI G'TCGA_C

Cuts at:    855     855 
   Size:       3818
 
SapI GCTCTTCn'nnn_

Cuts at:   1841    2051    1841 
   Size:        210    3608
 
SbfI CC_TGCA'GG

Cuts at:    865     865 
   Size:       3818
 
ScaI AGT'ACT

Cuts at:   3335    3335 
   Size:       3818
 
SfcI C'TryA_G

Cuts at:     33     298     397     861    1546    2539    2730      33 
   Size:        265      99     464     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     464     265     191      99
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    251     251 
   Size:       3818
 
SfoI GGC'GCC

Cuts at:   1250    1498    1250 
   Size:        248    3570
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3818
 
SmaI CCC'GGG

Cuts at:    258     840     258 
   Size:        582    3236
 
SmlI C'TyrA_G

Cuts at:    317     973    1888    2380    2642     317 
   Size:        656     915     492     262    1493

  Fragments arranged by size: 

                1493     915     656     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3818
 
SphI G_CATG'C

Cuts at:    871    1371     871 
   Size:        500    3318
 
StyI C'CwwG_G

Cuts at:    247     540     740     952    1306     247 
   Size:        293     200     212     354    2759

  Fragments arranged by size: 

                2759     354     293     212     200
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    428    1401    1534    1993    2202     428 
   Size:        973     133     459     209    2044

  Fragments arranged by size: 

                2044     973     459     209     133
 
TatI w'GTAC_w

Cuts at:   3333    3333 
   Size:       3818
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3335    3367    3335 
   Size:         32    3786
 
Tth111I GACn'n_nGTC

Cuts at:   1615    1615 
   Size:       3818
 
XbaI T'CTAG_A

Cuts at:    849     849 
   Size:       3818
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1313    1313 
   Size:       3818
 
XhoI C'TCGA_G

Cuts at:    317     317 
   Size:       3818
 
XmaI C'CCGG_G

Cuts at:    256     838     256 
   Size:        582    3236
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PciI     PmeI     PpiI   PspOMI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfiI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AleI     AlfI     AscI    AvrII
     BaeI     BbsI    BbvCI     BcgI    BfrBI    BmgBI     BmtI    BseRI
     BsgI    BsiWI    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I
   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI
    PspXI     PsrI    SacII    SanDI    SexAI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     XmnI     ZraI
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