Restriction map for pF1KE0106
##################################
# NO CUT ENZYMES (  139 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BamHI       BbeI        
BbrPI       BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaWI       BseAI       
BsiWI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DrdI        
Eam1105I    Eco47III    Eco81I      EcoO65I     EcoRV       
EcoT22I     FbaI        FseI        FspI        HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MaeII       MamI        MfeI        MluI        MroI        
MseI        MslI        MunI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tru9I       Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccII         347  CGCG                 CG^CG
AcsI          715  GAATTC               [GA]^AATT[CT]
AfaI          825  GTAC                 GT^AC
ApaI          625  GGGCCC               GGGCC^C
ApoI          715  GAATTC               [GA]^AATT[CT]
BbsI          450  GAAGAC               GAAGAC(2/6)
BbvCI         602  CCTCAGC              CCTCAGC(-5/-2)
BcnI          410  CCCGG                CC^[GC]GG
BpuAI         450  GAAGAC               GAAGAC(2/6)
BseRI         134  CTCCTC               GAGGAG(10/8)
BsgI          274  CTGCAC               GTGCAG(16/14)
BsiEI         287  CGGCCG               CG[GA][CT]^CG
BspMI         293  ACCTGC               ACCTGC(4/8)
BsrFI         248  GCCGGC               [GA]^CCGG[CT]
BsrI          267  CCAGT                ACTGG(1/-1)
BstUI         347  CGCG                 CG^CG
BstXI         828  CCAAGAGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         548  GGATCT               [GA]^GATC[CT]
Cfr10I        248  GCCGGC               [GA]^CCGG[CT]
DraIII        772  CACGGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI           28  CCGTGG               C^C[GA][CT]GG
EagI          287  CGGCCG               C^GGCCG
EarI          476  CTCTTC               CTCTTC(1/4)
EclXI         287  CGGCCG               C^GGCCG
Eco52I        287  CGGCCG               C^GGCCG
EcoRI         715  GAATTC               G^AATTC
EcoT14I       332  CCAAGG               C^C[AT][AT]GG
FauI          300  CCCGC                CCCGC(4/6)
HaeII         245  AGCGCC               [GA]GCGC^[CT]
HphI          102  GGTGA                GGTGA(8/7)
Ksp632I       476  CTCTTC               CTCTTC(1/4)
MaeIII        103  GTGAC                ^GT[ACGT]AC
MflI          548  GGATCT               [GA]^GATC[CT]
MvnI          347  CGCG                 CG^CG
NaeI          248  GCCGGC               GCC^GGC
NciI          410  CCCGG                CC^[GC]GG
NgoMIV        248  GCCGGC               G^CCGGC
RsaI          825  GTAC                 GT^AC
SapI          475  GCTCTTC              GCTCTTC(1/4)
StyI          332  CCAAGG               C^C[AT][AT]GG
Tsp45I        103  GTGAC                ^GT[GC]AC
TspRI         351  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoII         548  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AcyI          105  GACGCC               G[GA]^CG[CT]C
              365  GACGCC              
AlwI          357  GATCC                GGATC(4/5)
              548  GGATC               
BalI          432  TGGCCA               TGG^CCA
              837  TGGCCA              
BanI          325  GGCACC               G^G[CT][GA]CC
              532  GGCACC              
BanII         227  GAGCCC               G[GA]GC[CT]^C
              625  GGGCCC              
BpmI          137  CTCCAG               CTGGAG(16/14)
              766  CTGGAG              
BsaHI         105  GACGCC               G[GA]^CG[CT]C
              365  GACGCC              
BsaI           33  GAGACC               GGTCTC(1/5)
              221  GAGACC              
BsmFI          66  GGGAC                GGGAC(10/14)
              611  GGGAC               
BstAPI        732  GCAGACTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              771  GCACGGAGTGC         
CfoI          246  GCGC                 GCG^C
              348  GCGC                
DpnII         357  GATC                 ^GATC
              549  GATC                
HapII         249  CCGG                 C^CGG
              411  CCGG                
HgaI          105  GACGC                GACGC(5/10)
              365  GACGC               
HhaI          246  GCGC                 GCG^C
              348  GCGC                
Hin1I         105  GACGCC               G[GA]^CG[CT]C
              365  GACGCC              
HinP1I        246  GCGC                 G^CGC
              348  GCGC                
HincII        484  GTCAAC               GT[CT]^[GA]AC
              523  GTTGAC              
HindII        484  GTCAAC               GT[CT]^[GA]AC
              523  GTTGAC              
HpaII         249  CCGG                 C^CGG
              411  CCGG                
MboI          357  GATC                 ^GATC
              549  GATC                
MluNI         432  TGGCCA               TGG^CCA
              837  TGGCCA              
MscI          432  TGGCCA               TGG^CCA
              837  TGGCCA              
MspI          249  CCGG                 C^CGG
              411  CCGG                
NdeII         357  GATC                 ^GATC
              549  GATC                
NlaIII        150  CATG                 CATG^
              430  CATG                
PstI           57  CTGCAG               CTGCA^G
              730  CTGCAG              
Sau3AI        357  GATC                 ^GATC
              549  GATC                
SmlI          313  CTCAAG               C^T[CT][GA]AG
              748  CTCAAG              
TaqI          219  TCGA                 T^CGA
              761  TCGA                
TfiI          426  GATTC                G^A[AT]TC
              468  GATTC               
Tsp509I       388  AATT                 ^AATT
              716  AATT                
TthHB8I       219  TCGA                 T^CGA
              761  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AciI        AluI        AlwNI       AspHI       AvaII       
BbvI        BmyI        BsaJI       BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DraII       
EaeI        EcoNI       EcoO109I    EcoRII      Fnu4HI      
FokI        HaeIII      HinfI       ItaI        MboII       
MnlI        MspA1I      MvaI        MwoI        NlaIV       
PleI        PpuMI       PvuII       Sau96I      ScrFI       
SfaNI       SfcI        TseI        
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