Restriction map for pF1KB8892
##################################
# NO CUT ENZYMES (  141 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvrII       
BamHI       BanI        BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfrI        
BglII       BlnI        BpuAI       BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiHKAI     BsiWI       BsmFI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrDI       
BsrGI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
ClaI        DraI        DraIII      DrdI        Eam1105I    
Eco47III    Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HincII      
HindII      HindIII     HpaI        KasI        KpnI        
MaeIII      MamI        MfeI        MflI        MluI        
MroI        MslI        MunI        NarI        NcoI        
NdeI        NheI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsaI        SacI        SalI        SapI        SbfI        
ScaI        SexAI       SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        TfiI        Tsp45I      Tsp509I     
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AcyI          223  GACGCC               G[GA]^CG[CT]C
AlwI          472  GATCC                GGATC(4/5)
ApaI          573  GGGCCC               GGGCC^C
Asp700I       553  GAAGGTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII          54  TGCGCA               TGC^GCA
BalI          449  TGGCCA               TGG^CCA
BfaI          412  CTAG                 C^TAG
BlpI          138  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      138  GCTGAGC              GC^T[ACGT]AGC
BsaHI         223  GACGCC               G[GA]^CG[CT]C
BsmAI         312  GAGAC                GTCTC(1/5)
BsmBI         312  GAGACG               CGTCTC(1/5)
BsmI          337  GCATTC               GAATGC(1/-1)
BsrBI         307  GAGCGG               CCGCTC(-3/-3)
BsrI          577  CCAGT                ACTGG(1/-1)
BssSI           7  CTCGTG               CACGAG(-5/-1)
BstAPI         57  GCACCCCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII         138  GCTGAGC              GC^T[ACGT]AGC
CpoI           87  CGGTCCG              CG^G[AT]CCG
DsaI          188  CCGCGG               C^C[GA][CT]GG
EcoT14I       452  CCAAGG               C^C[AT][AT]GG
FspI           54  TGCGCA               TGC^GCA
Hin1I         223  GACGCC               G[GA]^CG[CT]C
HinfI         402  GACTC                G^A[ACGT]TC
KspI          188  CCGCGG               CCGC^GG
MaeI          412  CTAG                 C^TAG
MluNI         449  TGGCCA               TGG^CCA
MscI          449  TGGCCA               TGG^CCA
NlaIII         63  CATG                 CATG^
NotI          190  GCGGCCGC             GC^GGCCGC
PleI          402  GACTC                GAGTC(4/5)
PpuMI         294  AGGACCC              [GA]G^G[AT]CC[CT]
RsrII          87  CGGTCCG              CG^G[AT]CCG
SacII         188  CCGCGG               CCGC^GG
SfiI          268  GGCCGTCTTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          244  CCCGGG               CCC^GGG
SmlI          496  CTCAAG               C^T[CT][GA]AG
StyI          452  CCAAGG               C^C[AT][AT]GG
TaqI          581  TCGA                 T^CGA
TspRI         318  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       581  TCGA                 T^CGA
XmaCI         244  CCCGGG               C^CCGGG
XmaI          244  CCCGGG               C^CCGGG
XmnI          553  GAAGGTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AvaI          244  CCCGGG               C^[CT]CG[GA]G
              475  CCCGAG              
BglI          269  GCCGTCTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              481  GCCTTCAAGGC         
BpmI          253  CTGGAG               CTGGAG(16/14)
              631  CTGGAG              
BsoBI         244  CCCGGG               C^[CT]CG[GA]G
              475  CCCGAG              
DpnII         396  GATC                 ^GATC
              472  GATC                
DraII         115  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              294  AGGACCC             
EarI           82  CTCTTC               CTCTTC(1/4)
              585  GAAGAG              
EcoO109I      115  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              294  AGGACCC             
HaeII         258  GGCGCT               [GA]GCGC^[CT]
              627  GGCGCT              
HphI           30  TCACC                GGTGA(8/7)
               39  TCACC               
Ksp632I        82  CTCTTC               CTCTTC(1/4)
              585  GAAGAG              
MaeII         368  ACGT                 A^CGT
              443  ACGT                
MboI          396  GATC                 ^GATC
              472  GATC                
MboII         585  GAAGA                GAAGA(8/7)
               83  TCTTC               
MspA1I        188  CCGCGG               C[AC]G^C[GT]G
              194  CCGCTG              
NaeI          278  GCCGGC               GCC^GGC
              372  GCCGGC              
NdeII         396  GATC                 ^GATC
              472  GATC                
NgoMIV        278  GCCGGC               G^CCGGC
              372  GCCGGC              
NlaIV         295  GGACCC               GG[ACGT]^[ACGT]CC
              573  GGGCCC              
PstI          197  CTGCAG               CTGCA^G
              541  CTGCAG              
Sau3AI        396  GATC                 ^GATC
              472  GATC                
SfaNI         457  GATGC                GCATC(5/9)
              359  GCATC               
SfcI          197  CTGCAG               C^T[GA][CT]AG
              541  CTGCAG              
StuI          229  AGGCCT               AGG^CCT
              479  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AccII       AciI        AluI        AvaII       BanII       
BbvI        BcnI        BmyI        BsaJI       BsiEI       
BsiYI       BslI        Bsp1286I    BsrFI       BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        EaeI        EagI        
EclXI       Eco52I      EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
HinP1I      HpaII       ItaI        MnlI        MseI        
MspI        MvaI        MvnI        MwoI        NciI        
Sau96I      ScrFI       Tru9I       TseI        
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