Restriction map for pF1KB8892
 (Linear) (Six-base) MAPSORT of: KIBB8892.seq  Check: 3131  from: 1  to: 645



KIBB8892.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

ApaI G_GGCC'C

Cuts at:      0     577     645 
   Size:        577      68
 
AvaI C'yCGr_G

Cuts at:      0     244     475     645 
   Size:        244     231     170

  Fragments arranged by size: 

                 244     231     170
 
BanII G_rGCy'C

Cuts at:      0     145     244     577     645 
   Size:        145      99     333      68

  Fragments arranged by size: 

                 333     145      99      68
 
BglI GCCn_nnn'nGGC

Cuts at:      0     275     487     645 
   Size:        275     212     158

  Fragments arranged by size: 

                 275     212     158
 
BlpI GC'TnA_GC

Cuts at:      0     139     645 
   Size:        139     506
 
Bme1580I G_kGCm'C

Cuts at:      0     577     645 
   Size:        577      68
 
BmgBI CAC'GTC

Cuts at:      0     444     645 
   Size:        444     201
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     571     645 
   Size:        571      74
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     274     645 
   Size:        274     371
 
Bpu10I CC'TnA_GC

Cuts at:      0     291     645 
   Size:        291     354
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     481     645 
   Size:        481     164
 
BsaHI Gr'CG_yC

Cuts at:      0     224     645 
   Size:        224     421
 
BsiEI CG_ry'CG

Cuts at:      0     194     270     377     516     645 
   Size:        194      76     107     139     129

  Fragments arranged by size: 

                 194     139     129     107      76
 
BsmI GAATG_Cn'

Cuts at:      0     337     645 
   Size:        337     308
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     306     645 
   Size:        306     339
 
Bsp1286I G_dGCh'C

Cuts at:      0     145     244     577     645 
   Size:        145      99     333      68

  Fragments arranged by size: 

                 333     145      99      68
 
BsrBI CCG'CTC

Cuts at:      0     309     645 
   Size:        309     336
 
BsrFI r'CCGG_y

Cuts at:      0     278     372     604     645 
   Size:        278      94     232      41

  Fragments arranged by size: 

                 278     232      94      41
 
BssSI C'ACGA_G

Cuts at:      0       7     645 
   Size:          7     638
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      63     645 
   Size:         63     582
 
BtgI C'CryG_G

Cuts at:      0     188     645 
   Size:        188     457
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     411     645 
   Size:        411     234
 
EaeI y'GGCC_r

Cuts at:      0     191     267     276     374     449     513     610     645 
   Size:        191      76       9      98      75      64      97      35

  Fragments arranged by size: 

                 191      98      97      76      75      64      35       9
            
 
EagI C'GGCC_G

Cuts at:      0     191     267     374     513     645 
   Size:        191      76     107     139     132

  Fragments arranged by size: 

                 191     139     132     107      76
 
EarI CTCTTCn'nnn_

Cuts at:      0      88     580     645 
   Size:         88     492      65

  Fragments arranged by size: 

                 492      88      65
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     297     520     645 
   Size:        297     223     125

  Fragments arranged by size: 

                 297     223     125
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     274     645 
   Size:        274     371
 
EcoO109I rG'GnC_Cy

Cuts at:      0     116     295     645 
   Size:        116     179     350

  Fragments arranged by size: 

                 350     179     116
 
FspI TGC'GCA

Cuts at:      0      56     645 
   Size:         56     589
 
HaeII r_GCGC'y

Cuts at:      0     262     631     645 
   Size:        262     369      14

  Fragments arranged by size: 

                 369     262      14
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     387     419     448     480     645 
   Size:        387      32      29      32     165

  Fragments arranged by size: 

                 387     165      32      32      29
 
MscI TGG'CCA

Cuts at:      0     451     645 
   Size:        451     194
 
MspA1I CmG'CkG

Cuts at:      0     190     196     645 
   Size:        190       6     449

  Fragments arranged by size: 

                 449     190       6
 
NaeI GCC'GGC

Cuts at:      0     280     374     645 
   Size:        280      94     271

  Fragments arranged by size: 

                 280     271      94
 
NgoMIV G'CCGG_C

Cuts at:      0     278     372     645 
   Size:        278      94     273

  Fragments arranged by size: 

                 278     273      94
 
NotI GC'GGCC_GC

Cuts at:      0     191     645 
   Size:        191     454
 
PasI CC'CwG_GG

Cuts at:      0     570     645 
   Size:        570      75
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      65      97     645 
   Size:         65      32     548

  Fragments arranged by size: 

                 548      65      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     295     645 
   Size:        295     350
 
PspOMI G'GGCC_C

Cuts at:      0     573     645 
   Size:        573      72
 
PstI C_TGCA'G

Cuts at:      0     201     545     645 
   Size:        201     344     100

  Fragments arranged by size: 

                 344     201     100
 
RsrII CG'GwC_CG

Cuts at:      0      88     645 
   Size:         88     557
 
SacII CC_GC'GG

Cuts at:      0     191     645 
   Size:        191     454
 
SfcI C'TryA_G

Cuts at:      0     197     541     645 
   Size:        197     344     104

  Fragments arranged by size: 

                 344     197     104
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     275     645 
   Size:        275     370
 
SmaI CCC'GGG

Cuts at:      0     246     645 
   Size:        246     399
 
SmlI C'TyrA_G

Cuts at:      0     496     645 
   Size:        496     149
 
StuI AGG'CCT

Cuts at:      0     231     481     645 
   Size:        231     250     164

  Fragments arranged by size: 

                 250     231     164
 
StyI C'CwwG_G

Cuts at:      0     452     645 
   Size:        452     193
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      76     645 
   Size:         76     569
 
XmaI C'CCGG_G

Cuts at:      0     244     645 
   Size:        244     401
 
XmnI GAAnn'nnTTC

Cuts at:      0     557     645 
   Size:        557      88
 

 Enzymes that do cut:

     ApaI     AvaI    BanII     BglI     BlpI Bme1580I    BmgBI     BmrI
     BpmI   Bpu10I    BpuEI    BsaHI    BsiEI     BsmI    BsmBI Bsp1286I
    BsrBI    BsrFI    BssSI   BstAPI     BtgI    BtgZI     EaeI     EagI
     EarI     EciI  Eco57MI EcoO109I     FspI    HaeII    Hin4I     MscI
   MspA1I     NaeI   NgoMIV     NotI     PasI     PpiI    PpuMI   PspOMI
     PstI    RsrII    SacII     SfcI     SfiI     SmaI     SmlI     StuI
     StyI    TaqII     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI    BsiWI    BspEI    BspHI    BspMI    BsrDI
    BsrGI   BssHII    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MslI     NarI     NcoI
     NdeI     NheI     NruI     NsiI     NspI     PacI     PciI    PflMI
     PfoI     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI     PvuI
    PvuII     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     ZraI
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