Restriction map for pF1KB6499
##################################
# NO CUT ENZYMES (  191 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AfaI        AflII       AflIII      AgeI        AhdI        
AluI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanII       BbrPI       BbsI        BbvCI       
BbvI        BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BpmI        BpuAI       BsaAI       BsaBI       BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsiYI       
BslI        BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
Cfr10I      ClaI        CpoI        DpnII       DraI        
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        FspI        HaeIII      
HincII      HindII      HindIII     HpaI        KpnI        
KspI        MaeI        MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MslI        MspA1I      MunI        NaeI        
NciI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NlaIII      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      SbfI        
ScaI        SexAI       SfcI        SfiI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        Tru9I       TseI        
Tsp509I     Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccI          262  GTCTAC               GT^[AC][GT]AC
AcyI          107  GGCGCC               G[GA]^CG[CT]C
AspHI         223  GAGCAC               G[AT]GC[AT]^C
AvaII          63  GGTCC                G^G[AT]CC
BanI          107  GGCGCC               G^G[CT][GA]CC
BbeI          107  GGCGCC               GGCGC^C
BlpI           46  GCTAAGC              GC^T[ACGT]AGC
BmyI          223  GAGCAC               G[AGT]GC[ACT]^C
Bpu1102I       46  GCTAAGC              GC^T[ACGT]AGC
BsaHI         107  GGCGCC               G[GA]^CG[CT]C
BsaI          145  GGTCTC               GGTCTC(1/5)
BsaJI          81  CCAGGG               C^C[ACGT][ACGT]GG
BseRI         157  GAGGAG               GAGGAG(10/8)
BsiHKAI       223  GAGCAC               G[AT]GC[AT]^C
Bsp1286I      223  GAGCAC               G[AGT]GC[ACT]^C
BsrI           30  ACTGG                ACTGG(1/-1)
BssKI          81  CCAGG                ^CC[ACGT]GG
BstNI          81  CCAGG                CC^[AT]GG
CelII          46  GCTAAGC              GC^T[ACGT]AGC
Cfr13I         63  GGTCC                G^G[ACGT]CC
DdeI           47  CTAAG                C^T[ACGT]AG
DraII          62  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EarI          273  GAAGAG               CTCTTC(1/4)
EcoO109I       62  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRII         81  CCAGG                ^CC[AT]GG
HapII         105  CCGG                 C^CGG
Hin1I         107  GGCGCC               G[GA]^CG[CT]C
HpaII         105  CCGG                 C^CGG
HphI           54  TCACC                GGTGA(8/7)
KasI          107  GGCGCC               G^GCGCC
Ksp632I       273  GAAGAG               CTCTTC(1/4)
MaeII         194  ACGT                 A^CGT
MboII         273  GAAGA                GAAGA(8/7)
MspI          105  CCGG                 C^CGG
MvaI           81  CCAGG                CC^[AT]GG
NarI          107  GGCGCC               GG^CGCC
NlaIV         107  GGCGCC               GG[ACGT]^[ACGT]CC
PleI          162  GACTC                GAGTC(4/5)
PpuMI          62  AGGTCCT              [GA]G^G[AT]CC[CT]
Sau96I         63  GGTCC                G^G[ACGT]CC
ScrFI          81  CCAGG                CC^[ACGT]GG
SfaNI         205  GATGC                GCATC(5/9)
SfoI          107  GGCGCC               GGC^GCC
TfiI          198  GATTC                G^A[AT]TC
TspRI         242  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

BsmFI          27  GGGAC                GGGAC(10/14)
              282  GGGAC               
Cac8I          96  GCCTGC               GC[ACGT]^[ACGT]GC
              225  GCACGC              
FauI          122  GCGGG                CCCGC(4/6)
              167  GCGGG               
Fnu4HI         11  GCGGC                GC^[ACGT]GC
              305  GCGGC               
HaeII          43  GGCGCT               [GA]GCGC^[CT]
              107  GGCGCC              
HgaI          284  GACGC                GACGC(5/10)
               51  GCGTC               
HinfI         162  GACTC                G^A[ACGT]TC
              198  GATTC               
ItaI           11  GCGGC                GC^[ACGT]GC
              305  GCGGC               
MnlI           41  GAGG                 CCTC(7/6)
              157  GAGG                
MwoI           11  GCGGCAAAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              299  GCTTCGGCGGC         


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

AccII       AciI        BsmAI       BstF5I      BstUI       
CfoI        FokI        HhaI        HinP1I      MaeIII      
MvnI        Tsp45I      
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