Restriction map for pF1KB7347
 (Circular) (Six-base) MAPSORT of: pF1KB7347.seq  Check: 1818  from: 1  to: 6422



pF1KB7347  6422 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1201    1201 
   Size:       6422
 
AatII G_ACGT'C

Cuts at:   2294    2294 
   Size:       6422
 
AccI GT'mk_AC

Cuts at:   2870    3460    2870 
   Size:        590    5832
 
Acc65I G'GTAC_C

Cuts at:   1399    3438    1399 
   Size:       2039    4383
 
AclI AA'CG_TT

Cuts at:   6262    6262 
   Size:       6422
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1079    1813    1972    2128    3262    4247    4679    5426    5694 
   Size:        734     159     156    1134     985     432     747     268

Cuts at:   5694    1079 
   Size:       1807

  Fragments arranged by size: 

                1807    1134     985     747     734     432     268     159
                 156
 
AfeI AGC'GCT

Cuts at:    404     877     404 
   Size:        473    5949
 
AflIII A'CryG_T

Cuts at:   2960    3774    4878    5855    2960 
   Size:        814    1104     977    3527

  Fragments arranged by size: 

                3527    1104     977     814
 
AgeI A'CCGG_T

Cuts at:   4810    4810 
   Size:       6422
 
AleI CACnn'nnGTG

Cuts at:    829     829 
   Size:       6422
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   3058    3370    3058 
   Size:        312    6110
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2014    2046    2014 
   Size:         32    6390
 
AlwNI CAG_nnn'CTG

Cuts at:    684    2676    3727    5294     684 
   Size:       1992    1051    1567    1812

  Fragments arranged by size: 

                1992    1812    1567    1051
 
ApaI G_GGCC'C

Cuts at:    204     289     729     818    1020     204 
   Size:         85     440      89     202    5606

  Fragments arranged by size: 

                5606     440     202      89      85
 
ApaLI G'TGCA_C

Cuts at:   5192    5192 
   Size:       6422
 
ApoI r'AATT_y

Cuts at:   1798    3426    5775    6031    1798 
   Size:       1628    2349     256    2189

  Fragments arranged by size: 

                2349    2189    1628     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6422
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6422
 
AvaI C'yCGr_G

Cuts at:    148     197     314     412     728     785    1376    1910    2365 
   Size:         49     117      98     316      57     591     534     455

Cuts at:   2365    2725    2910    3048    3204    3370    3442     148 
   Size:        360     185     138     156     166      72    3128

  Fragments arranged by size: 

                3128     591     534     455     360     316     185     166
                 156     138     117      98      72      57      49
 
AvrII C'CTAG_G

Cuts at:   1287    1287 
   Size:       6422
 
BamHI G'GATC_C

Cuts at:   1368    3447    1368 
   Size:       2079    4343
 
BanI G'GyrC_C

Cuts at:    549     583    1150    1399    1571    1660    1702    2433    3253 
   Size:         34     567     249     172      89      42     731     820

Cuts at:   3253    3438    3852    4100    4135     549 
   Size:        185     414     248      35    2836

  Fragments arranged by size: 

                2836     820     731     567     414     249     248     185
                 172      89      42      35      34
 
BanII G_rGCy'C

Cuts at:    155     177     204     289     321     436     729     767     786 
   Size:         22      27      85      32     115     293      38      19

Cuts at:    786     818    1020    1092    1307    1384    1868    1984    2360 
   Size:         32     202      72     215      77     484     116     376

Cuts at:   2360    2395    2726    2801    3436    4466     155 
   Size:         35     331      75     635    1030    2111

  Fragments arranged by size: 

                2111    1030     635     484     376     331     293     215
                 202     116     115      85      77      75      72      38
                  35      32      32      27      22      19
 
BbeI G_GCGC'C

Cuts at:   3257    3856    4104    3257 
   Size:        599     248    5575

  Fragments arranged by size: 

                5575     599     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    921    1161    2551    2656    2710     921 
   Size:        240    1390     105      54    4633

  Fragments arranged by size: 

                4633    1390     240     105      54
 
BbvCI CC'TCA_GC

Cuts at:   2693    2693 
   Size:       6422
 
BciVI GTATCCnnnnn_n'

Cuts at:   2458    4320    5081    6422    2458 
   Size:       1862     761    1341    2458

  Fragments arranged by size: 

                2458    1862    1341     761
 
BclI T'GATC_A

Cuts at:    833    3942     833 
   Size:       3109    3313
 
BglI GCCn_nnn'nGGC

Cuts at:    343     380     790    1555     343 
   Size:         37     410     765    5210

  Fragments arranged by size: 

                5210     765     410      37
 
BglII A'GATC_T

Cuts at:   3937    5974    3937 
   Size:       2037    4385
 
BlpI GC'TnA_GC

Cuts at:   3534    3534 
   Size:       6422
 
Bme1580I G_kGCm'C

Cuts at:    204     289     554     586     729     818    1020    1663    1707 
   Size:         85     265      32     143      89     202     643      44

Cuts at:   1707    2340    2992    4047    4140    5196     204 
   Size:        633     652    1055      93    1056    1430

  Fragments arranged by size: 

                1430    1056    1055     652     643     633     265     202
                 143      93      89      85      44      32
 
BmrI ACTGGGnnnn_n'

Cuts at:   3634    3715    4050    6194    3634 
   Size:         81     335    2144    3862

  Fragments arranged by size: 

                3862    2144     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1128    2414    2604    4789    1128 
   Size:       1286     190    2185    2761

  Fragments arranged by size: 

                2761    2185    1286     190
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    800     907    1510    1843    2284    2374    2599    2620    2653 
   Size:        107     603     333     441      90     225      21      33

Cuts at:   2653    3409     800 
   Size:        756    3813

  Fragments arranged by size: 

                3813     756     603     441     333     225     107      90
                  33      21
 
Bpu10I CC'TnA_GC

Cuts at:   2248    2693    2248 
   Size:        445    5977
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1076    2131    3192    3598    4477    4969    5267    1076 
   Size:       1055    1061     406     879     492     298    2231

  Fragments arranged by size: 

                2231    1061    1055     879     492     406     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1140    1334    1602    1965    6126    1140 
   Size:        194     268     363    4161    1436

  Fragments arranged by size: 

                4161    1436     363     268     194
 
BsaAI yAC'GTr

Cuts at:   3165    3775    3165 
   Size:        610    5812
 
BsaBI GATnn'nnATC

Cuts at:      6    3936    5973       6 
   Size:       3930    2037     455

  Fragments arranged by size: 

                3930    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    427    2291    2894    3254    3853    4101    4803    6299     427 
   Size:       1864     603     360     599     248     702    1496     550

  Fragments arranged by size: 

                1864    1496     702     603     599     550     360     248
            
 
BsaWI w'CCGG_w

Cuts at:    141     486    1228    2199    3611    4132    4810    5084    5231 
   Size:        345     742     971    1412     521     678     274     147

Cuts at:   5231    5642     141 
   Size:        411     921

  Fragments arranged by size: 

                1412     971     921     742     678     521     411     345
                 274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1223    1253    1769    1799    2713    2743    1223 
   Size:         30     516      30     914      30    4902

  Fragments arranged by size: 

                4902     914     516      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    883     913    1307    1315    1987    2641     883 
   Size:         30     394       8     672     654    4664

  Fragments arranged by size: 

                4664     672     654     394      30       8
 
BseYI C'CCAG_C

Cuts at:    438     647     858     903    1004    1543    1735    1830    2256 
   Size:        209     211      45     101     539     192      95     426

Cuts at:   2256    2468    2921    3847    4605    5182    5678     438 
   Size:        212     453     926     758     577     496    1182

  Fragments arranged by size: 

                1182     926     758     577     539     496     453     426
                 212     211     209     192     101      95      45
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1956    2062    2398    3028    1956 
   Size:        106     336     630    5350

  Fragments arranged by size: 

                5350     630     336     106
 
BsiEI CG_ry'CG

Cuts at:    104    3403    3964    4010    5218     104 
   Size:       3299     561      46    1208    1308

  Fragments arranged by size: 

                3299    1308    1208     561      46
 
BsiHKAI G_wGCw'C

Cuts at:   1034    1868    1970    2360    3178    3198    3436    4214    4404 
   Size:        834     102     390     818      20     238     778     190

Cuts at:   4404    5196    1034 
   Size:        792    2260

  Fragments arranged by size: 

                2260     834     818     792     778     390     238     190
                 102      20
 
BsmI GAATG_Cn'

Cuts at:    640     640 
   Size:       6422
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1289    1289 
   Size:       6422
 
Bsp1286I G_dGCh'C

Cuts at:    155     177     204     289     321     436     554     586     729 
   Size:         22      27      85      32     115     118      32     143

Cuts at:    729     767     786     818    1020    1034    1092    1307    1384 
   Size:         38      19      32     202      14      58     215      77

Cuts at:   1384    1663    1707    1868    1970    1984    2340    2360    2395 
   Size:        279      44     161     102      14     356      20      35

Cuts at:   2395    2726    2801    2992    3178    3198    3436    4047    4140 
   Size:        331      75     191     186      20     238     611      93

Cuts at:   4140    4214    4404    4466    5196     155 
   Size:         74     190      62     730    1381

  Fragments arranged by size: 

                1381     730     611     356     331     279     238     215
                 202     191     190     186     161     143     118     115
                 102      93      85      77      75      74      62      58
                  44      38      35      32      32      32      27      22
                  20      20      19      14      14
 
BspEI T'CCGG_A

Cuts at:    141    1228    5642     141 
   Size:       1087    4414     921

  Fragments arranged by size: 

                4414    1087     921
 
BspHI T'CATG_A

Cuts at:   5598    5598 
   Size:       6422
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1122    1201    1254    2825    3329    3472    3686    3988    4369 
   Size:         79      53    1571     504     143     214     302     381

Cuts at:   4369    1122 
   Size:       3175

  Fragments arranged by size: 

                3175    1571     504     381     302     214     143      79
                  53
 
BsrBI CCG'CTC

Cuts at:     50     130     219    2215    3375    4714    4768    6252    6417 
   Size:         80      89    1996    1160    1339      54    1484     165

Cuts at:   6417      50 
   Size:         55

  Fragments arranged by size: 

                1996    1484    1339    1160     165      89      80      55
                  54
 
BsrDI GCAATG_nn'

Cuts at:   4334    4334 
   Size:       6422
 
BsrFI r'CCGG_y

Cuts at:    506    1343    1396    1574    2284    2657    4420    4601    4810 
   Size:        837      53     178     710     373    1763     181     209

Cuts at:   4810     506 
   Size:       2118

  Fragments arranged by size: 

                2118    1763     837     710     373     209     181     178
                  53
 
BssHII G'CGCG_C

Cuts at:    652     652 
   Size:       6422
 
BssSI C'ACGA_G

Cuts at:    478    1449    4693    5051     478 
   Size:        971    3244     358    1849

  Fragments arranged by size: 

                3244    1849     971     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    852    2385    3799     852 
   Size:       1533    1414    3475

  Fragments arranged by size: 

                3475    1533    1414
 
BstBI TT'CG_AA

Cuts at:   4783    4783 
   Size:       6422
 
BstEII G'GTnAC_C

Cuts at:    666     666 
   Size:       6422
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1654    2929    1654 
   Size:       1275    5147
 
BstYI r'GATC_y

Cuts at:      1    1368    2604    3362    3447    3914    3937    4272    4518 
   Size:       1367    1236     758      85     467      23     335     246

Cuts at:   4518    5530    5616    5628    5974       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1367    1236    1012     758     467     449     346     335
                 246      86      85      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    796    3014     796 
   Size:       2218    4204
 
BtgI C'CryG_G

Cuts at:    587     611     713     758    1625    2160    2166    2423    2498 
   Size:         24     102      45     867     535       6     257      75

Cuts at:   2498    2984    3088    3342     587 
   Size:        486     104     254    3667

  Fragments arranged by size: 

                3667     867     535     486     257     254     104     102
                  75      45      24       6
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   4372    4553    4716    4372 
   Size:        181     163    6078

  Fragments arranged by size: 

                6078     181     163
 
BtsI GCAGTG_nn'

Cuts at:    836    1968    2340    2470    3665     836 
   Size:       1132     372     130    1195    3593

  Fragments arranged by size: 

                3593    1195    1132     372     130
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   1169    1204    1169 
   Size:         35    6387
 
DraI TTT'AAA

Cuts at:   3421    3421 
   Size:       6422
 
DraIII CAC_nnn'GTG

Cuts at:    852     852 
   Size:       6422
 
DrdI GACnn_nn'nnGTC

Cuts at:   4128    4986    4128 
   Size:        858    5564
 
EaeI y'GGCC_r

Cuts at:    332     344     508     983    2163    2282    2303    3400    4007 
   Size:         12     164     475    1180     119      21    1097     607

Cuts at:   4007    4181    4572    4599    5944     332 
   Size:        174     391      27    1345     810

  Fragments arranged by size: 

                1345    1180    1097     810     607     475     391     174
                 164     119      27      21      12
 
EagI C'GGCC_G

Cuts at:   3400    4007    3400 
   Size:        607    5815
 
EarI CTCTTCn'nnn_

Cuts at:   1991    4445    4655    1991 
   Size:       2454     210    3758

  Fragments arranged by size: 

                3758    2454     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    249     592    1383    1816    4630    4940    5086    6232     249 
   Size:        343     791     433    2814     310     146    1146     439

  Fragments arranged by size: 

                2814    1146     791     439     433     343     310     146
            
 
EcoICRI GAG'CTC

Cuts at:   1866    2358    3434    1866 
   Size:        492    1076    4854

  Fragments arranged by size: 

                4854    1076     492
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    800     907    1079    1510    1813    1843    1972    2128    2284 
   Size:        107     172     431     303      30     129     156     156

Cuts at:   2284    2374    2599    2620    2653    3262    3409    4247    4679 
   Size:         90     225      21      33     609     147     838     432

Cuts at:   4679    5426    5694     800 
   Size:        747     268    1528

  Fragments arranged by size: 

                1528     838     747     609     432     431     303     268
                 225     172     156     156     147     129     107      90
                  33      30      21
 
EcoNI CCTnn'n_nnAGG

Cuts at:    799     886    1939    2059     799 
   Size:         87    1053     120    5162

  Fragments arranged by size: 

                5162    1053     120      87
 
EcoO109I rG'GnC_Cy

Cuts at:    201     279     286     681     725     792    1016    1266    1284 
   Size:         78       7     395      44      67     224     250      18

Cuts at:   1284    1547    1924    1992    2253    3561     201 
   Size:        263     377      68     261    1308    3062

  Fragments arranged by size: 

                3062    1308     395     377     263     261     250     224
                  78      68      67      44      18       7
 
EcoRI G'AATT_C

Cuts at:   3426    3426 
   Size:       6422
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1863    4441    1863 
   Size:       2578    3844
 
FspI TGC'GCA

Cuts at:   1084    1262    3028    4203    1084 
   Size:        178    1766    1175    3303

  Fragments arranged by size: 

                3303    1766    1175     178
 
FspAI rTGC'GCAy

Cuts at:   3028    3028 
   Size:       6422
 
HaeII r_GCGC'y

Cuts at:    406     879    1101    2271    3257    3856    4104    5126    6105 
   Size:        473     222    1170     986     599     248    1022     979

Cuts at:   6105     406 
   Size:        723

  Fragments arranged by size: 

                1170    1022     986     979     723     599     473     248
                 222
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2129    2161    2664    2696    4264    4294    4296    4326    2129 
   Size:         32     503      32    1568      30       2      30    4225

  Fragments arranged by size: 

                4225    1568     503      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   3117    3461    3117 
   Size:        344    6078
 
KasI G'GCGC_C

Cuts at:   3253    3852    4100    3253 
   Size:        599     248    5575

  Fragments arranged by size: 

                5575     599     248
 
KpnI G_GTAC'C

Cuts at:   1403    3442    1403 
   Size:       2039    4383
 
MluI A'CGCG_T

Cuts at:   5855    5855 
   Size:       6422
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   5093    5277    5093 
   Size:        184    6238
 
MscI TGG'CCA

Cuts at:   2305    4183    2305 
   Size:       1878    4544
 
MslI CAynn'nnrTG

Cuts at:    829     841    1360    1591    1652    2311    3163    4411    4538 
   Size:         12     519     231      61     659     852    1248     127

Cuts at:   4538     829 
   Size:       2713

  Fragments arranged by size: 

                2713    1248     852     659     519     231     127      61
                  12
 
MspA1I CmG'CkG

Cuts at:    613    1237    1730    2226    2925    3090    3533    3847    4207 
   Size:        624     493     496     699     165     443     314     360

Cuts at:   4207    5220    5465     613 
   Size:       1013     245    1570

  Fragments arranged by size: 

                1570    1013     699     624     496     493     443     360
                 314     245     165
 
NaeI GCC'GGC

Cuts at:    508    2286    4603     508 
   Size:       1778    2317    2327

  Fragments arranged by size: 

                2327    2317    1778
 
NarI GG'CG_CC

Cuts at:   3254    3853    4101    3254 
   Size:        599     248    5575

  Fragments arranged by size: 

                5575     599     248
 
NcoI C'CATG_G

Cuts at:    758    2423    2498     758 
   Size:       1665      75    4682

  Fragments arranged by size: 

                4682    1665      75
 
NdeI CA'TA_TG

Cuts at:   3008    3008 
   Size:       6422
 
NgoMIV G'CCGG_C

Cuts at:    506    2284    4601     506 
   Size:       1778    2317    2327

  Fragments arranged by size: 

                2327    2317    1778
 
NspI r_CATG'y

Cuts at:   1365    1590    1716    2964    3003    3475    3975    4882    1365 
   Size:        225     126    1248      39     472     500     907    2905

  Fragments arranged by size: 

                2905    1248     907     500     472     225     126      39
            
 
PasI CC'CwG_GG

Cuts at:    290     554     818    1680    1804     290 
   Size:        264     264     862     124    4908

  Fragments arranged by size: 

                4908     862     264     264     124
 
PciI A'CATG_T

Cuts at:   2960    4878    2960 
   Size:       1918    4504
 
PflMI CCAn_nnn'nTGG

Cuts at:   1147    1746    1147 
   Size:        599    5823
 
PfoI T'CCnGG_A

Cuts at:    884    1253    1859    2612     884 
   Size:        369     606     753    4694

  Fragments arranged by size: 

                4694     753     606     369
 
PmeI GTTT'AAAC

Cuts at:   3421    3421 
   Size:       6422
 
PmlI CAC'GTG

Cuts at:   3165    3165 
   Size:       6422
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5561    5593    5561 
   Size:         32    6390
 
PpuMI rG'GwC_Cy

Cuts at:    279     681    1266    1284    1992    2253     279 
   Size:        402     585      18     708     261    4448

  Fragments arranged by size: 

                4448     708     585     402     261      18
 
PspOMI G'GGCC_C

Cuts at:    200     285     725     814    1016     200 
   Size:         85     440      89     202    5606

  Fragments arranged by size: 

                5606     440     202      89      85
 
PstI C_TGCA'G

Cuts at:    245    1130    3359    3469    4154     245 
   Size:        885    2229     110     685    2513

  Fragments arranged by size: 

                2513    2229     885     685     110
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6422
 
PvuII CAG'CTG

Cuts at:   1237    2226    3847    4207    1237 
   Size:        989    1621     360    3452

  Fragments arranged by size: 

                3452    1621     989     360
 
RsrII CG'GwC_CG

Cuts at:   4617    4617 
   Size:       6422
 
SacI G_AGCT'C

Cuts at:   1868    2360    3436    1868 
   Size:        492    1076    4854

  Fragments arranged by size: 

                4854    1076     492
 
SacII CC_GC'GG

Cuts at:    614    3091     614 
   Size:       2477    3945
 
SalI G'TCGA_C

Cuts at:   3459    3459 
   Size:       6422
 
SanDI GG'GwC_CC

Cuts at:   2253    2253 
   Size:       6422
 
SapI GCTCTTCn'nnn_

Cuts at:   4445    4655    4445 
   Size:        210    6212
 
SbfI CC_TGCA'GG

Cuts at:   3359    3469    3359 
   Size:        110    6312
 
ScaI AGT'ACT

Cuts at:   2587    2731    5939    2587 
   Size:        144    3208    3070

  Fragments arranged by size: 

                3208    3070     144
 
SexAI A'CCwGG_T

Cuts at:   3212    3212 
   Size:       6422
 
SfcI C'TryA_G

Cuts at:     33     241    1126    3355    3465    4150    5143    5334      33 
   Size:        208     885    2229     110     685     993     191    1121

  Fragments arranged by size: 

                2229    1121     993     885     685     208     191     110
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   1555    1555 
   Size:       6422
 
SfoI GGC'GCC

Cuts at:   3255    3854    4102    3255 
   Size:        599     248    5575

  Fragments arranged by size: 

                5575     599     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6422
 
SmaI CCC'GGG

Cuts at:    150     199     730     787    1378    3444     150 
   Size:         49     531      57     591    2066    3128

  Fragments arranged by size: 

                3128    2066     591     531      57      49
 
SmlI C'TyrA_G

Cuts at:   1091    2146    3171    3577    4492    4984    5246    1091 
   Size:       1055    1025     406     915     492     262    2267

  Fragments arranged by size: 

                2267    1055    1025     915     492     406     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6422
 
SphI G_CATG'C

Cuts at:   3003    3475    3975    3003 
   Size:        472     500    5450

  Fragments arranged by size: 

                5450     500     472
 
StuI AGG'CCT

Cuts at:    979    2698    3041     979 
   Size:       1719     343    4360

  Fragments arranged by size: 

                4360    1719     343
 
StyI C'CwwG_G

Cuts at:    758     767     980    1287    2414    2423    2498    3556    3910 
   Size:          9     213     307    1127       9      75    1058     354

Cuts at:   3910     758 
   Size:       3270

  Fragments arranged by size: 

                3270    1127    1058     354     307     213      75       9
                   9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    678     850     928    1212    1664    1690    4005    4138    4597 
   Size:        172      78     284     452      26    2315     133     459

Cuts at:   4597    4806     678 
   Size:        209    2294

  Fragments arranged by size: 

                2315    2294     459     452     284     209     172     133
                  78      26
 
TatI w'GTAC_w

Cuts at:   2585    2729    2750    5937    2585 
   Size:        144      21    3187    3070

  Fragments arranged by size: 

                3187    3070     144      21
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1223    1255    2212    2244    2957    2989    3177    3209    5939 
   Size:         32     957      32     713      32     188      32    2730

Cuts at:   5939    5971    1223 
   Size:         32    1674

  Fragments arranged by size: 

                2730    1674     957     713     188      32      32      32
                  32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1165    3112    4219    1165 
   Size:       1947    1107    3368

  Fragments arranged by size: 

                3368    1947    1107
 
XbaI T'CTAG_A

Cuts at:   3453    3453 
   Size:       6422
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    765    1362    1743    2808    2964    3917     765 
   Size:        597     381    1065     156     953    3270

  Fragments arranged by size: 

                3270    1065     953     597     381     156
 
XmaI C'CCGG_G

Cuts at:    148     197     728     785    1376    3442     148 
   Size:         49     531      57     591    2066    3128

  Fragments arranged by size: 

                3128    2066     591     531      57      49
 
XmnI GAAnn'nnTTC

Cuts at:   1798    1798 
   Size:       6422
 
ZraI GAC'GTC

Cuts at:   2292    2292 
   Size:       6422
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    FspAI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI   PspOMI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

    AflII     AhdI     AscI     BaeI     BcgI    BfrBI    BmgBI     BmtI
    BsiWI    BsrGI  BstZ17I     ClaI    EcoRV     FseI  HindIII     HpaI
     MfeI     NheI     NotI     NruI     NsiI     PacI    PshAI     PsiI
    PspXI     PsrI    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI
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