Restriction map for pF1KB7347
 (Linear) (Six-base) MAPSORT of: KIBB7347.seq  Check: 7089  from: 1  to: 3309



KIBB7347.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1093    3309 
   Size:       1093    2216
 
AatII G_ACGT'C

Cuts at:      0    2186    3309 
   Size:       2186    1123
 
AccI GT'mk_AC

Cuts at:      0    2762    3309 
   Size:       2762     547
 
Acc65I G'GTAC_C

Cuts at:      0    1291    3309 
   Size:       1291    2018
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     971    1705    1864    2020    3154    3309 
   Size:        971     734     159     156    1134     155

  Fragments arranged by size: 

                1134     971     734     159     156     155
 
AfeI AGC'GCT

Cuts at:      0     296     769    3309 
   Size:        296     473    2540

  Fragments arranged by size: 

                2540     473     296
 
AflIII A'CryG_T

Cuts at:      0    2852    3309 
   Size:       2852     457
 
AleI CACnn'nnGTG

Cuts at:      0     721    3309 
   Size:        721    2588
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2950    3262    3309 
   Size:       2950     312      47

  Fragments arranged by size: 

                2950     312      47
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1906    1938    3309 
   Size:       1906      32    1371

  Fragments arranged by size: 

                1906    1371      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     576    2568    3309 
   Size:        576    1992     741

  Fragments arranged by size: 

                1992     741     576
 
ApaI G_GGCC'C

Cuts at:      0      96     181     621     710     912    3309 
   Size:         96      85     440      89     202    2397

  Fragments arranged by size: 

                2397     440     202      96      89      85
 
ApoI r'AATT_y

Cuts at:      0    1690    3309 
   Size:       1690    1619
 
AvaI C'yCGr_G

Cuts at:      0      40      89     206     304     620     677    1268    1802 
   Size:         40      49     117      98     316      57     591     534

Cuts at:   1802    2257    2617    2802    2940    3096    3262    3309 
   Size:        455     360     185     138     156     166      47

  Fragments arranged by size: 

                 591     534     455     360     316     185     166     156
                 138     117      98      57      49      47      40
 
AvrII C'CTAG_G

Cuts at:      0    1179    3309 
   Size:       1179    2130
 
BamHI G'GATC_C

Cuts at:      0    1260    3309 
   Size:       1260    2049
 
BanI G'GyrC_C

Cuts at:      0     441     475    1042    1291    1463    1552    1594    2325 
   Size:        441      34     567     249     172      89      42     731

Cuts at:   2325    3145    3309 
   Size:        820     164

  Fragments arranged by size: 

                 820     731     567     441     249     172     164      89
                  42      34
 
BanII G_rGCy'C

Cuts at:      0      47      69      96     181     213     328     621     659 
   Size:         47      22      27      85      32     115     293      38

Cuts at:    659     678     710     912     984    1199    1276    1760    1876 
   Size:         19      32     202      72     215      77     484     116

Cuts at:   1876    2252    2287    2618    2693    3309 
   Size:        376      35     331      75     616

  Fragments arranged by size: 

                 616     484     376     331     293     215     202     116
                 115      85      77      75      72      47      38      35
                  32      32      27      22      19
 
BbeI G_GCGC'C

Cuts at:      0    3149    3309 
   Size:       3149     160
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     813    1053    2443    2548    2602    3309 
   Size:        813     240    1390     105      54     707

  Fragments arranged by size: 

                1390     813     707     240     105      54
 
BbvCI CC'TCA_GC

Cuts at:      0    2585    3309 
   Size:       2585     724
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2350    3309 
   Size:       2350     959
 
BclI T'GATC_A

Cuts at:      0     725    3309 
   Size:        725    2584
 
BglI GCCn_nnn'nGGC

Cuts at:      0     235     272     682    1447    3309 
   Size:        235      37     410     765    1862

  Fragments arranged by size: 

                1862     765     410     235      37
 
Bme1580I G_kGCm'C

Cuts at:      0      96     181     446     478     621     710     912    1555 
   Size:         96      85     265      32     143      89     202     643

Cuts at:   1555    1599    2232    2884    3309 
   Size:         44     633     652     425

  Fragments arranged by size: 

                 652     643     633     425     265     202     143      96
                  89      85      44      32
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1020    2306    2496    3309 
   Size:       1020    1286     190     813

  Fragments arranged by size: 

                1286    1020     813     190
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     692     799    1402    1735    2176    2266    2491    2512 
   Size:        692     107     603     333     441      90     225      21

Cuts at:   2512    2545    3301    3309 
   Size:         33     756       8

  Fragments arranged by size: 

                 756     692     603     441     333     225     107      90
                  33      21       8
 
Bpu10I CC'TnA_GC

Cuts at:      0    2140    2585    3309 
   Size:       2140     445     724

  Fragments arranged by size: 

                2140     724     445
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     968    2023    3084    3309 
   Size:        968    1055    1061     225

  Fragments arranged by size: 

                1061    1055     968     225
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1032    1226    1494    1857    3309 
   Size:       1032     194     268     363    1452

  Fragments arranged by size: 

                1452    1032     363     268     194
 
BsaAI yAC'GTr

Cuts at:      0    3057    3309 
   Size:       3057     252
 
BsaHI Gr'CG_yC

Cuts at:      0     319    2183    2786    3146    3309 
   Size:        319    1864     603     360     163

  Fragments arranged by size: 

                1864     603     360     319     163
 
BsaWI w'CCGG_w

Cuts at:      0      33     378    1120    2091    3309 
   Size:         33     345     742     971    1218

  Fragments arranged by size: 

                1218     971     742     345      33
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1115    1145    1661    1691    2605    2635    3309 
   Size:       1115      30     516      30     914      30     674

  Fragments arranged by size: 

                1115     914     674     516      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     775     805    1199    1207    1879    2533    3309 
   Size:        775      30     394       8     672     654     776

  Fragments arranged by size: 

                 776     775     672     654     394      30       8
 
BseYI C'CCAG_C

Cuts at:      0     330     539     750     795     896    1435    1627    1722 
   Size:        330     209     211      45     101     539     192      95

Cuts at:   1722    2148    2360    2813    3309 
   Size:        426     212     453     496

  Fragments arranged by size: 

                 539     496     453     426     330     212     211     209
                 192     101      95      45
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1848    1954    2290    2920    3309 
   Size:       1848     106     336     630     389

  Fragments arranged by size: 

                1848     630     389     336     106
 
BsiEI CG_ry'CG

Cuts at:      0    3295    3309 
   Size:       3295      14
 
BsiHKAI G_wGCw'C

Cuts at:      0     926    1760    1862    2252    3070    3090    3309 
   Size:        926     834     102     390     818      20     219

  Fragments arranged by size: 

                 926     834     818     390     219     102      20
 
BsmI GAATG_Cn'

Cuts at:      0     532    3309 
   Size:        532    2777
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1181    3309 
   Size:       1181    2128
 
Bsp1286I G_dGCh'C

Cuts at:      0      47      69      96     181     213     328     446     478 
   Size:         47      22      27      85      32     115     118      32

Cuts at:    478     621     659     678     710     912     926     984    1199 
   Size:        143      38      19      32     202      14      58     215

Cuts at:   1199    1276    1555    1599    1760    1862    1876    2232    2252 
   Size:         77     279      44     161     102      14     356      20

Cuts at:   2252    2287    2618    2693    2884    3070    3090    3309 
   Size:         35     331      75     191     186      20     219

  Fragments arranged by size: 

                 356     331     279     219     215     202     191     186
                 161     143     118     115     102      85      77      75
                  58      47      44      38      35      32      32      32
                  27      22      20      20      19      14      14
 
BspEI T'CCGG_A

Cuts at:      0      33    1120    3309 
   Size:         33    1087    2189

  Fragments arranged by size: 

                2189    1087      33
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1014    1093    1146    2717    3221    3309 
   Size:       1014      79      53    1571     504      88

  Fragments arranged by size: 

                1571    1014     504      88      79      53
 
BsrBI CCG'CTC

Cuts at:      0      22     111    2107    3267    3309 
   Size:         22      89    1996    1160      42

  Fragments arranged by size: 

                1996    1160      89      42      22
 
BsrFI r'CCGG_y

Cuts at:      0     398    1235    1288    1466    2176    2549    3309 
   Size:        398     837      53     178     710     373     760

  Fragments arranged by size: 

                 837     760     710     398     373     178      53
 
BssHII G'CGCG_C

Cuts at:      0     544    3309 
   Size:        544    2765
 
BssSI C'ACGA_G

Cuts at:      0     370    1341    3309 
   Size:        370     971    1968

  Fragments arranged by size: 

                1968     971     370
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     744    2277    3309 
   Size:        744    1533    1032

  Fragments arranged by size: 

                1533    1032     744
 
BstEII G'GTnAC_C

Cuts at:      0     558    3309 
   Size:        558    2751
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1546    2821    3309 
   Size:       1546    1275     488

  Fragments arranged by size: 

                1546    1275     488
 
BstYI r'GATC_y

Cuts at:      0    1260    2496    3254    3309 
   Size:       1260    1236     758      55

  Fragments arranged by size: 

                1260    1236     758      55
 
Bsu36I CC'TnA_GG

Cuts at:      0     688    2906    3309 
   Size:        688    2218     403

  Fragments arranged by size: 

                2218     688     403
 
BtgI C'CryG_G

Cuts at:      0     479     503     605     650    1517    2052    2058    2315 
   Size:        479      24     102      45     867     535       6     257

Cuts at:   2315    2390    2876    2980    3234    3309 
   Size:         75     486     104     254      75

  Fragments arranged by size: 

                 867     535     486     479     257     254     104     102
                  75      75      45      24       6
 
BtsI GCAGTG_nn'

Cuts at:      0     728    1860    2232    2362    3309 
   Size:        728    1132     372     130     947

  Fragments arranged by size: 

                1132     947     728     372     130
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1061    1096    3309 
   Size:       1061      35    2213

  Fragments arranged by size: 

                2213    1061      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     744    3309 
   Size:        744    2565
 
EaeI y'GGCC_r

Cuts at:      0     224     236     400     875    2055    2174    2195    3292 
   Size:        224      12     164     475    1180     119      21    1097

Cuts at:   3292    3309 
   Size:         17

  Fragments arranged by size: 

                1180    1097     475     224     164     119      21      17
                  12
 
EagI C'GGCC_G

Cuts at:      0    3292    3309 
   Size:       3292      17
 
EarI CTCTTCn'nnn_

Cuts at:      0    1883    3309 
   Size:       1883    1426
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     141     484    1275    1708    3309 
   Size:        141     343     791     433    1601

  Fragments arranged by size: 

                1601     791     433     343     141
 
EcoICRI GAG'CTC

Cuts at:      0    1758    2250    3309 
   Size:       1758     492    1059

  Fragments arranged by size: 

                1758    1059     492
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     692     799     971    1402    1705    1735    1864    2020 
   Size:        692     107     172     431     303      30     129     156

Cuts at:   2020    2176    2266    2491    2512    2545    3154    3301    3309 
   Size:        156      90     225      21      33     609     147       8

  Fragments arranged by size: 

                 692     609     431     303     225     172     156     156
                 147     129     107      90      33      30      21       8
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     691     778    1831    1951    3309 
   Size:        691      87    1053     120    1358

  Fragments arranged by size: 

                1358    1053     691     120      87
 
EcoO109I rG'GnC_Cy

Cuts at:      0      93     171     178     573     617     684     908    1158 
   Size:         93      78       7     395      44      67     224     250

Cuts at:   1158    1176    1439    1816    1884    2145    3309 
   Size:         18     263     377      68     261    1164

  Fragments arranged by size: 

                1164     395     377     263     261     250     224      93
                  78      68      67      44      18       7
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1755    3309 
   Size:       1755    1554
 
FspI TGC'GCA

Cuts at:      0     976    1154    2920    3309 
   Size:        976     178    1766     389

  Fragments arranged by size: 

                1766     976     389     178
 
FspAI rTGC'GCAy

Cuts at:      0    2920    3309 
   Size:       2920     389
 
HaeII r_GCGC'y

Cuts at:      0     298     771     993    2163    3149    3309 
   Size:        298     473     222    1170     986     160

  Fragments arranged by size: 

                1170     986     473     298     222     160
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2021    2053    2556    2588    3309 
   Size:       2021      32     503      32     721

  Fragments arranged by size: 

                2021     721     503      32      32
 
HincII GTy'rAC

Cuts at:      0    3009    3309 
   Size:       3009     300
 
KasI G'GCGC_C

Cuts at:      0    3145    3309 
   Size:       3145     164
 
KpnI G_GTAC'C

Cuts at:      0    1295    3309 
   Size:       1295    2014
 
MscI TGG'CCA

Cuts at:      0    2197    3309 
   Size:       2197    1112
 
MslI CAynn'nnrTG

Cuts at:      0     721     733    1252    1483    1544    2203    3055    3309 
   Size:        721      12     519     231      61     659     852     254

  Fragments arranged by size: 

                 852     721     659     519     254     231      61      12
            
 
MspA1I CmG'CkG

Cuts at:      0     505    1129    1622    2118    2817    2982    3309 
   Size:        505     624     493     496     699     165     327

  Fragments arranged by size: 

                 699     624     505     496     493     327     165
 
NaeI GCC'GGC

Cuts at:      0     400    2178    3309 
   Size:        400    1778    1131

  Fragments arranged by size: 

                1778    1131     400
 
NarI GG'CG_CC

Cuts at:      0    3146    3309 
   Size:       3146     163
 
NcoI C'CATG_G

Cuts at:      0     650    2315    2390    3309 
   Size:        650    1665      75     919

  Fragments arranged by size: 

                1665     919     650      75
 
NdeI CA'TA_TG

Cuts at:      0    2900    3309 
   Size:       2900     409
 
NgoMIV G'CCGG_C

Cuts at:      0     398    2176    3309 
   Size:        398    1778    1133

  Fragments arranged by size: 

                1778    1133     398
 
NspI r_CATG'y

Cuts at:      0    1257    1482    1608    2856    2895    3309 
   Size:       1257     225     126    1248      39     414

  Fragments arranged by size: 

                1257    1248     414     225     126      39
 
PasI CC'CwG_GG

Cuts at:      0     182     446     710    1572    1696    3309 
   Size:        182     264     264     862     124    1613

  Fragments arranged by size: 

                1613     862     264     264     182     124
 
PciI A'CATG_T

Cuts at:      0    2852    3309 
   Size:       2852     457
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1039    1638    3309 
   Size:       1039     599    1671

  Fragments arranged by size: 

                1671    1039     599
 
PfoI T'CCnGG_A

Cuts at:      0     776    1145    1751    2504    3309 
   Size:        776     369     606     753     805

  Fragments arranged by size: 

                 805     776     753     606     369
 
PmlI CAC'GTG

Cuts at:      0    3057    3309 
   Size:       3057     252
 
PpuMI rG'GwC_Cy

Cuts at:      0     171     573    1158    1176    1884    2145    3309 
   Size:        171     402     585      18     708     261    1164

  Fragments arranged by size: 

                1164     708     585     402     261     171      18
 
PspOMI G'GGCC_C

Cuts at:      0      92     177     617     706     908    3309 
   Size:         92      85     440      89     202    2401

  Fragments arranged by size: 

                2401     440     202      92      89      85
 
PstI C_TGCA'G

Cuts at:      0     137    1022    3251    3309 
   Size:        137     885    2229      58

  Fragments arranged by size: 

                2229     885     137      58
 
PvuII CAG'CTG

Cuts at:      0    1129    2118    3309 
   Size:       1129     989    1191

  Fragments arranged by size: 

                1191    1129     989
 
SacI G_AGCT'C

Cuts at:      0    1760    2252    3309 
   Size:       1760     492    1057

  Fragments arranged by size: 

                1760    1057     492
 
SacII CC_GC'GG

Cuts at:      0     506    2983    3309 
   Size:        506    2477     326

  Fragments arranged by size: 

                2477     506     326
 
SanDI GG'GwC_CC

Cuts at:      0    2145    3309 
   Size:       2145    1164
 
SbfI CC_TGCA'GG

Cuts at:      0    3251    3309 
   Size:       3251      58
 
ScaI AGT'ACT

Cuts at:      0    2479    2623    3309 
   Size:       2479     144     686

  Fragments arranged by size: 

                2479     686     144
 
SexAI A'CCwGG_T

Cuts at:      0    3104    3309 
   Size:       3104     205
 
SfcI C'TryA_G

Cuts at:      0     133    1018    3247    3309 
   Size:        133     885    2229      62

  Fragments arranged by size: 

                2229     885     133      62
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1447    3309 
   Size:       1447    1862
 
SfoI GGC'GCC

Cuts at:      0    3147    3309 
   Size:       3147     162
 
SmaI CCC'GGG

Cuts at:      0      42      91     622     679    1270    3309 
   Size:         42      49     531      57     591    2039

  Fragments arranged by size: 

                2039     591     531      57      49      42
 
SmlI C'TyrA_G

Cuts at:      0     983    2038    3063    3309 
   Size:        983    1055    1025     246

  Fragments arranged by size: 

                1055    1025     983     246
 
SphI G_CATG'C

Cuts at:      0    2895    3309 
   Size:       2895     414
 
StuI AGG'CCT

Cuts at:      0     871    2590    2933    3309 
   Size:        871    1719     343     376

  Fragments arranged by size: 

                1719     871     376     343
 
StyI C'CwwG_G

Cuts at:      0     650     659     872    1179    2306    2315    2390    3309 
   Size:        650       9     213     307    1127       9      75     919

  Fragments arranged by size: 

                1127     919     650     307     213      75       9       9
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     570     742     820    1104    1556    1582    3309 
   Size:        570     172      78     284     452      26    1727

  Fragments arranged by size: 

                1727     570     452     284     172      78      26
 
TatI w'GTAC_w

Cuts at:      0    2477    2621    2642    3309 
   Size:       2477     144      21     667

  Fragments arranged by size: 

                2477     667     144      21
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1115    1147    2104    2136    2849    2881    3069    3101 
   Size:       1115      32     957      32     713      32     188      32

Cuts at:   3101    3309 
   Size:        208

  Fragments arranged by size: 

                1115     957     713     208     188      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1057    3004    3309 
   Size:       1057    1947     305

  Fragments arranged by size: 

                1947    1057     305
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     657    1254    1635    2700    2856    3309 
   Size:        657     597     381    1065     156     453

  Fragments arranged by size: 

                1065     657     597     453     381     156
 
XmaI C'CCGG_G

Cuts at:      0      40      89     620     677    1268    3309 
   Size:         40      49     531      57     591    2041

  Fragments arranged by size: 

                2041     591     531      57      49      40
 
XmnI GAAnn'nnTTC

Cuts at:      0    1690    3309 
   Size:       1690    1619
 
ZraI GAC'GTC

Cuts at:      0    2184    3309 
   Size:       2184    1125
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AfeI   AflIII     AleI
     AlfI     AloI    AlwNI     ApaI     ApoI     AvaI    AvrII    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI     BglI
 Bme1580I     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI   BssHII    BssSI
   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI    CspCI
   DraIII     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI     FspI    FspAI    HaeII    Hin4I   HincII     KasI
     KpnI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NspI     PasI     PciI    PflMI     PfoI     PmlI    PpuMI
   PspOMI     PstI    PvuII     SacI    SacII    SanDI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SmaI     SmlI     SphI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AclI    AflII     AgeI     AhdI    ApaLI     AscI     AseI    AsiSI
     BaeI     BcgI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI
    BsaBI    BsiWI    BspHI    BsrDI    BsrGI    BstBI  BstZ17I    BtgZI
     ClaI     DraI     DrdI    EcoRI    EcoRV     FseI  HindIII     HpaI
     MfeI     MluI     MmeI     NheI     NotI     NruI     NsiI     PacI
     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
     SalI     SapI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI
     SwaI     XbaI     XhoI
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