Restriction map for pF1KE0563
##################################
# NO CUT ENZYMES (  167 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AspI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BpuAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmFI       BsmI        BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrFI       BsrI        BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CfoI        Cfr10I      ClaI        CpoI        
DraI        DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HindIII     
HpaI        HpaII       KasI        KpnI        KspI        
MaeIII      MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MslI        MspI        
MunI        MvnI        NaeI        NarI        NciI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        Tsp45I      TspRI       Tth111I     XhoII       
XmaCI       XmaI        


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# ONE CUT ENZYMES (   56 enzymes) #
###################################

AcsI           97  AAATTT               [GA]^AATT[CT]
AlwI          315  GATCC                GGATC(4/5)
ApoI           97  AAATTT               [GA]^AATT[CT]
Asp700I       651  GAAGATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          513  CTCGAG               C^[CT]CG[GA]G
BanII         266  GAGCCC               G[GA]GC[CT]^C
BfaI          103  CTAG                 C^TAG
BlpI          456  GCTGAGC              GC^T[ACGT]AGC
BmyI          266  GAGCCC               G[AGT]GC[ACT]^C
Bpu1102I      456  GCTGAGC              GC^T[ACGT]AGC
BsaAI         496  TACGTG               [CT]AC^GT[GA]
BsgI          196  GTGCAG               GTGCAG(16/14)
BsoBI         513  CTCGAG               C^[CT]CG[GA]G
Bsp1286I      266  GAGCCC               G[AGT]GC[ACT]^C
Bsp1407I      281  TGTACA               T^GTACA
BspMI         569  ACCTGC               ACCTGC(4/8)
BsrDI         222  CATTGC               GCAATG(2/0)
BsrGI         281  TGTACA               T^GTACA
Cac8I         226  GCCAGC               GC[ACGT]^[ACGT]GC
CelII         456  GCTGAGC              GC^T[ACGT]AGC
Cfr13I        546  GGCCC                G^G[ACGT]CC
DdeI          457  CTGAG                C^T[ACGT]AG
DpnII         315  GATC                 ^GATC
DraII         545  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DraIII        475  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I      545  AGGCCCT              [GA]G^G[ACGT]CC[CT]
HaeIII        546  GGCC                 GG^CC
HincII        335  GTCAAC               GT[CT]^[GA]AC
HindII        335  GTCAAC               GT[CT]^[GA]AC
MaeI          103  CTAG                 C^TAG
MaeII         497  ACGT                 A^CGT
MboI          315  GATC                 ^GATC
MspA1I         74  CAGCTG               C[AC]G^C[GT]G
NcoI          620  CCATGG               C^CATGG
NdeI          330  CATATG               CA^TATG
NdeII         315  GATC                 ^GATC
NlaIV          62  GGCTCC               GG[ACGT]^[ACGT]CC
PaeR7I        513  CTCGAG               C^TCGAG
PflMI          53  CCATTCGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         511  GACTCGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII          74  CAGCTG               CAG^CTG
SapI          502  GAAGAGC              GCTCTTC(1/4)
Sau3AI        315  GATC                 ^GATC
Sau96I        546  GGCCC                G^G[ACGT]CC
SexAI         476  ACCTGGT              A^CC[AT]GGT
SfaNI         352  GATGC                GCATC(5/9)
SmlI          513  CTCGAG               C^T[CT][GA]AG
SspBI         281  TGTACA               T^GTACA
TaqI          514  TCGA                 T^CGA
Tsp509I        98  AATT                 ^AATT
TthHB8I       514  TCGA                 T^CGA
Van91I         53  CCATTCGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          102  TCTAGA               T^CTAGA
XcmI          356  CCAAGACTGCCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          513  CTCGAG               C^TCGAG
XmnI          651  GAAGATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AfaI          163  GTAC                 GT^AC
              282  GTAC                
BpmI          525  CTCCAG               CTGGAG(16/14)
               77  CTGGAG              
BstF5I        185  GGATG                GGATG(2/0)
              624  GGATG               
DsaI          575  CCACGG               C^C[GA][CT]GG
              620  CCATGG              
EarI           91  GAAGAG               CTCTTC(1/4)
              502  GAAGAG              
EcoT14I       365  CCTTGG               C^C[AT][AT]GG
              620  CCATGG              
FokI          185  GGATG                GGATG(9/13)
              624  GGATG               
HphI          579  GGTGA                GGTGA(8/7)
              474  TCACC               
Ksp632I        91  GAAGAG               CTCTTC(1/4)
              502  GAAGAG              
MwoI          150  GCTGTTCCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              355  GCCAAGACTGC         
NlaIII        255  CATG                 CATG^
              621  CATG                
RsaI          163  GTAC                 GT^AC
              282  GTAC                
StyI          365  CCTTGG               C^C[AT][AT]GG
              620  CCATGG              
TfiI          106  GAATC                G^A[AT]TC
              560  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AluI        BbvI        BsaJI       BsiYI       BslI        
BssKI       BstNI       EcoRII      Fnu4HI      HinfI       
ItaI        MboII       MnlI        MseI        MvaI        
PleI        PstI        ScrFI       SfcI        Tru9I       
TseI        
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