Restriction map for pF1KE0563
 (Circular) (Six-base) MAPSORT of: pF1KE0563.seq  Check: 8224  from: 1  to: 3779



pF1KE0563  3779 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AccI GT'mk_AC

Cuts at:    817     817 
   Size:       3779
 
Acc65I G'GTAC_C

Cuts at:    795     795 
   Size:       3779
 
AclI AA'CG_TT

Cuts at:   3619    3619 
   Size:       3779
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    670    1604    2036    2783    3051     670 
   Size:        934     432     747     268    1398

  Fragments arranged by size: 

                1398     934     747     432     268
 
AflIII A'CryG_T

Cuts at:   1131    2235    3212    1131 
   Size:       1104     977    1698

  Fragments arranged by size: 

                1698    1104     977
 
AgeI A'CCGG_T

Cuts at:   2167    2167 
   Size:       3779
 
AlwNI CAG_nnn'CTG

Cuts at:   1084    2651    1084 
   Size:       1567    2212
 
ApaLI G'TGCA_C

Cuts at:   2549    2549 
   Size:       3779
 
ApoI r'AATT_y

Cuts at:    205     783    3132    3388     205 
   Size:        578    2349     256     596

  Fragments arranged by size: 

                2349     596     578     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3779
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3779
 
AvaI C'yCGr_G

Cuts at:    621     799     621 
   Size:        178    3601
 
BamHI G'GATC_C

Cuts at:    804     804 
   Size:       3779
 
BanI G'GyrC_C

Cuts at:    795    1209    1457    1492     795 
   Size:        414     248      35    3082

  Fragments arranged by size: 

                3082     414     248      35
 
BanII G_rGCy'C

Cuts at:    378     793    1823     378 
   Size:        415    1030    2334

  Fragments arranged by size: 

                2334    1030     415
 
BbeI G_GCGC'C

Cuts at:   1213    1461    1213 
   Size:        248    3531
 
BciVI GTATCCnnnnn_n'

Cuts at:   1677    2438    3779    1677 
   Size:        761    1341    1677

  Fragments arranged by size: 

                1677    1341     761
 
BclI T'GATC_A

Cuts at:   1299    1299 
   Size:       3779
 
BglII A'GATC_T

Cuts at:   1294    3331    1294 
   Size:       2037    1742
 
BlpI GC'TnA_GC

Cuts at:    565     891     565 
   Size:        326    3453
 
Bme1580I G_kGCm'C

Cuts at:   1404    1497    2553    1404 
   Size:         93    1056    2630

  Fragments arranged by size: 

                2630    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    991    1072    1407    3551     991 
   Size:         81     335    2144    1219

  Fragments arranged by size: 

                2144    1219     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    636     740    2146     636 
   Size:        104    1406    2269

  Fragments arranged by size: 

                2269    1406     104
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    206     618     206 
   Size:        412    3367
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    955    1834    2326    2624     955 
   Size:        879     492     298    2110

  Fragments arranged by size: 

                2110     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3483    3483 
   Size:       3779
 
BsaAI yAC'GTr

Cuts at:    606    1132     606 
   Size:        526    3253
 
BsaBI GATnn'nnATC

Cuts at:      6    1293    3330       6 
   Size:       1287    2037     455

  Fragments arranged by size: 

                2037    1287     455
 
BsaHI Gr'CG_yC

Cuts at:   1210    1458    2160    3656    1210 
   Size:        248     702    1496    1333

  Fragments arranged by size: 

                1496    1333     702     248
 
BsaWI w'CCGG_w

Cuts at:    968    1489    2167    2441    2588    2999     968 
   Size:        521     678     274     147     411    1748

  Fragments arranged by size: 

                1748     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    569    1204    1962    2539    3035     569 
   Size:        635     758     577     496    1313

  Fragments arranged by size: 

                1313     758     635     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    325     325 
   Size:       3779
 
BsiEI CG_ry'CG

Cuts at:    104    1321    1367    2575     104 
   Size:       1217      46    1208    1308

  Fragments arranged by size: 

                1308    1217    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    793    1571    1761    2553     793 
   Size:        778     190     792    2019

  Fragments arranged by size: 

                2019     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    378     793    1404    1497    1571    1761    1823    2553     378 
   Size:        415     611      93      74     190      62     730    1604

  Fragments arranged by size: 

                1604     730     611     415     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2999    2999 
   Size:       3779
 
BspHI T'CATG_A

Cuts at:   2955    2955 
   Size:       3779
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    686     829    1043    1345    1726     686 
   Size:        143     214     302     381    2739

  Fragments arranged by size: 

                2739     381     302     214     143
 
BsrBI CCG'CTC

Cuts at:     50    2071    2125    3609    3774      50 
   Size:       2021      54    1484     165      55

  Fragments arranged by size: 

                2021    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    329    1691     329 
   Size:       1362    2417
 
BsrFI r'CCGG_y

Cuts at:   1777    1958    2167    1777 
   Size:        181     209    3389

  Fragments arranged by size: 

                3389     209     181
 
BsrGI T'GTAC_A

Cuts at:    389     389 
   Size:       3779
 
BssSI C'ACGA_G

Cuts at:   2050    2408    2050 
   Size:        358    3421
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1156    1156 
   Size:       3779
 
BstBI TT'CG_AA

Cuts at:   2140    2140 
   Size:       3779
 
BstYI r'GATC_y

Cuts at:      1     804    1271    1294    1629    1875    2887    2973    2985 
   Size:        803     467      23     335     246    1012      86      12

Cuts at:   2985    3331       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     803     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     683     728     107 
   Size:        576      45    3158

  Fragments arranged by size: 

                3158     576      45
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1729    1910    2073    1729 
   Size:        181     163    3435

  Fragments arranged by size: 

                3435     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1022    1022 
   Size:       3779
 
DraI TTT'AAA

Cuts at:    778     778 
   Size:       3779
 
DraIII CAC_nnn'GTG

Cuts at:    588     588 
   Size:       3779
 
DrdI GACnn_nn'nnGTC

Cuts at:   1485    2343    1485 
   Size:        858    2921
 
EaeI y'GGCC_r

Cuts at:   1364    1538    1929    1956    3301    1364 
   Size:        174     391      27    1345    1842

  Fragments arranged by size: 

                1842    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1364    1364 
   Size:       3779
 
EarI CTCTTCn'nnn_

Cuts at:    194     605    1802    2012     194 
   Size:        411    1197     210    1961

  Fragments arranged by size: 

                1961    1197     411     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1987    2297    2443    3589    1987 
   Size:        310     146    1146    2177

  Fragments arranged by size: 

                2177    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    791     791 
   Size:       3779
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    206     618     670    1604    2036    2783    3051     206 
   Size:        412      52     934     432     747     268     934

  Fragments arranged by size: 

                 934     934     747     432     412     268      52
 
EcoO109I rG'GnC_Cy

Cuts at:    654     918     654 
   Size:        264    3515
 
EcoRI G'AATT_C

Cuts at:    783     783 
   Size:       3779
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    489     565    1798     489 
   Size:         76    1233    2470

  Fragments arranged by size: 

                2470    1233      76
 
FspI TGC'GCA

Cuts at:   1560    1560 
   Size:       3779
 
HaeII r_GCGC'y

Cuts at:   1213    1461    2483    3462    1213 
   Size:        248    1022     979    1530

  Fragments arranged by size: 

                1530    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    724     756    1621    1651    1653    1683     724 
   Size:         32     865      30       2      30    2820

  Fragments arranged by size: 

                2820     865      32      30      30       2
 
HincII GTy'rAC

Cuts at:    445     818     445 
   Size:        373    3406
 
KasI G'GCGC_C

Cuts at:   1209    1457    1209 
   Size:        248    3531
 
KpnI G_GTAC'C

Cuts at:    799     799 
   Size:       3779
 
MluI A'CGCG_T

Cuts at:   3212    3212 
   Size:       3779
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    176    2450    2634     176 
   Size:       2274     184    1321

  Fragments arranged by size: 

                2274    1321     184
 
MscI TGG'CCA

Cuts at:   1540    1540 
   Size:       3779
 
MslI CAynn'nnrTG

Cuts at:   1768    1895    1768 
   Size:        127    3652
 
MspA1I CmG'CkG

Cuts at:    184     890    1204    1564    2577    2822     184 
   Size:        706     314     360    1013     245    1141

  Fragments arranged by size: 

                1141    1013     706     360     314     245
 
NaeI GCC'GGC

Cuts at:   1960    1960 
   Size:       3779
 
NarI GG'CG_CC

Cuts at:   1210    1458    1210 
   Size:        248    3531
 
NcoI C'CATG_G

Cuts at:    107     728     107 
   Size:        621    3158
 
NdeI CA'TA_TG

Cuts at:    439     439 
   Size:       3779
 
NgoMIV G'CCGG_C

Cuts at:   1958    1958 
   Size:       3779
 
NspI r_CATG'y

Cuts at:    832    1332    2239     832 
   Size:        500     907    2372

  Fragments arranged by size: 

                2372     907     500
 
PciI A'CATG_T

Cuts at:   2235    2235 
   Size:       3779
 
PflMI CCAn_nnn'nTGG

Cuts at:    167     167 
   Size:       3779
 
PmeI GTTT'AAAC

Cuts at:    778     778 
   Size:       3779
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2918    2950    2918 
   Size:         32    3747
 
PshAI GACnn'nnGTC

Cuts at:    623     623 
   Size:       3779
 
PspXI vC'TCGA_Gb

Cuts at:    621     621 
   Size:       3779
 
PstI C_TGCA'G

Cuts at:    183     222     704     826    1511     183 
   Size:         39     482     122     685    2451

  Fragments arranged by size: 

                2451     685     482     122      39
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3779
 
PvuII CAG'CTG

Cuts at:    184    1204    1564     184 
   Size:       1020     360    2399

  Fragments arranged by size: 

                2399    1020     360
 
RsrII CG'GwC_CG

Cuts at:   1974    1974 
   Size:       3779
 
SacI G_AGCT'C

Cuts at:    793     793 
   Size:       3779
 
SalI G'TCGA_C

Cuts at:    816     816 
   Size:       3779
 
SapI GCTCTTCn'nnn_

Cuts at:    605    1802    2012     605 
   Size:       1197     210    2372

  Fragments arranged by size: 

                2372    1197     210
 
SbfI CC_TGCA'GG

Cuts at:    826     826 
   Size:       3779
 
ScaI AGT'ACT

Cuts at:   3296    3296 
   Size:       3779
 
SexAI A'CCwGG_T

Cuts at:    584     584 
   Size:       3779
 
SfcI C'TryA_G

Cuts at:     33     179     218     700     822    1507    2500    2691      33 
   Size:        146      39     482     122     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     482     191     146     122      39
            
 
SfoI GGC'GCC

Cuts at:   1211    1459    1211 
   Size:        248    3531
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3779
 
SmaI CCC'GGG

Cuts at:    801     801 
   Size:       3779
 
SmlI C'TyrA_G

Cuts at:    621     934    1849    2341    2603     621 
   Size:        313     915     492     262    1797

  Fragments arranged by size: 

                1797     915     492     313     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3779
 
SphI G_CATG'C

Cuts at:    832    1332     832 
   Size:        500    3279
 
StyI C'CwwG_G

Cuts at:    107     473     728     913    1267     107 
   Size:        366     255     185     354    2619

  Fragments arranged by size: 

                2619     366     354     255     185
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1362    1495    1954    2163    1362 
   Size:        133     459     209    2978

  Fragments arranged by size: 

                2978     459     209     133
 
TatI w'GTAC_w

Cuts at:    389    3294     389 
   Size:       2905     874
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3296    3328    3296 
   Size:         32    3747
 
Tth111I GACn'n_nGTC

Cuts at:   1576    1576 
   Size:       3779
 
XbaI T'CTAG_A

Cuts at:    210     810     210 
   Size:        600    3179
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    471    1274     471 
   Size:        803    2976
 
XhoI C'TCGA_G

Cuts at:    621     621 
   Size:       3779
 
XmaI C'CCGG_G

Cuts at:    799     799 
   Size:       3779
 
XmnI GAAnn'nnTTC

Cuts at:    763     763 
   Size:       3779
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI
     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NspI     PciI    PflMI
     PmeI     PpiI    PshAI    PspXI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI
     BglI    BmgBI     BmtI   Bpu10I    BsaXI    BseRI    BsiWI     BsmI
    BsmBI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI
    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NheI
     NotI     NruI     NsiI     PacI     PasI     PfoI     PmlI    PpuMI
     PsiI   PspOMI     PsrI    SacII    SanDI     SfiI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     ZraI
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