(Linear) (Six-base) MAPSORT of: KIBB6468.seq  Check: 8261  from: 1  to: 1062



KIBB6468.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AccI GT'mk_AC

Cuts at:      0     218    1062 
   Size:        218     844
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     362     402    1062 
   Size:        362      40     660

  Fragments arranged by size: 

                 660     362      40
 
AleI CACnn'nnGTG

Cuts at:      0     145    1062 
   Size:        145     917
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      29     544    1062 
   Size:         29     515     518

  Fragments arranged by size: 

                 518     515      29
 
BbvCI CC'TCA_GC

Cuts at:      0     704    1062 
   Size:        704     358
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      36      70     975    1009    1062 
   Size:         36      34     905      34      53

  Fragments arranged by size: 

                 905      53      36      34      34
 
BfrBI ATG'CAT

Cuts at:      0     729    1062 
   Size:        729     333
 
BglII A'GATC_T

Cuts at:      0     924     953    1062 
   Size:        924      29     109

  Fragments arranged by size: 

                 924     109      29
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     483    1062 
   Size:        483     579
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     143     344    1062 
   Size:        143     201     718

  Fragments arranged by size: 

                 718     201     143
 
Bpu10I CC'TnA_GC

Cuts at:      0     704    1062 
   Size:        704     358
 
BsaBI GATnn'nnATC

Cuts at:      0     160    1062 
   Size:        160     902
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     727     852    1062 
   Size:        727     125     210

  Fragments arranged by size: 

                 727     210     125
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1015    1062 
   Size:       1015      47
 
BsiEI CG_ry'CG

Cuts at:      0      60    1062 
   Size:         60    1002
 
BsmI GAATG_Cn'

Cuts at:      0     731    1062 
   Size:        731     331
 
BspHI T'CATG_A

Cuts at:      0     168     353    1062 
   Size:        168     185     709

  Fragments arranged by size: 

                 709     185     168
 
BsrDI GCAATG_nn'

Cuts at:      0     242    1062 
   Size:        242     820
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     307    1062 
   Size:        307     755
 
BstYI r'GATC_y

Cuts at:      0     424     924     953    1062 
   Size:        424     500      29     109

  Fragments arranged by size: 

                 500     424     109      29
 
BtgI C'CryG_G

Cuts at:      0     260    1062 
   Size:        260     802
 
BtsI GCAGTG_nn'

Cuts at:      0      41     342    1062 
   Size:         41     301     720

  Fragments arranged by size: 

                 720     301      41
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     531     566    1062 
   Size:        531      35     496

  Fragments arranged by size: 

                 531     496      35
 
EaeI y'GGCC_r

Cuts at:      0      89     606    1062 
   Size:         89     517     456

  Fragments arranged by size: 

                 517     456      89
 
EarI CTCTTCn'nnn_

Cuts at:      0     802     884    1062 
   Size:        802      82     178

  Fragments arranged by size: 

                 802     178      82
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     143     344     362     402    1062 
   Size:        143     201      18      40     660

  Fragments arranged by size: 

                 660     201     143      40      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     820    1062 
   Size:        820     242
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     297     538    1062 
   Size:        297     241     524

  Fragments arranged by size: 

                 524     297     241
 
HaeII r_GCGC'y

Cuts at:      0      20    1062 
   Size:         20    1042
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     469     501     945     977    1062 
   Size:        469      32     444      32      85

  Fragments arranged by size: 

                 469     444      85      32      32
 
HincII GTy'rAC

Cuts at:      0     848    1062 
   Size:        848     214
 
HindIII A'AGCT_T

Cuts at:      0     410    1062 
   Size:        410     652
 
HpaI GTT'AAC

Cuts at:      0     848    1062 
   Size:        848     214
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     529    1062 
   Size:        529     533
 
MscI TGG'CCA

Cuts at:      0     608    1062 
   Size:        608     454
 
MslI CAynn'nnrTG

Cuts at:      0     145     173    1062 
   Size:        145      28     889

  Fragments arranged by size: 

                 889     145      28
 
MspA1I CmG'CkG

Cuts at:      0     898    1062 
   Size:        898     164
 
NcoI C'CATG_G

Cuts at:      0     260    1062 
   Size:        260     802
 
NdeI CA'TA_TG

Cuts at:      0     885    1062 
   Size:        885     177
 
NsiI A_TGCA'T

Cuts at:      0     731    1062 
   Size:        731     331
 
PvuII CAG'CTG

Cuts at:      0     898    1062 
   Size:        898     164
 
SfcI C'TryA_G

Cuts at:      0     219    1062 
   Size:        219     843
 
StyI C'CwwG_G

Cuts at:      0     260     947    1062 
   Size:        260     687     115

  Fragments arranged by size: 

                 687     260     115
 

 Enzymes that do cut:

     AccI     AcuI     AleI     BbsI    BbvCI     BcgI    BfrBI    BglII
     BmrI     BpmI   Bpu10I    BsaBI    BseRI     BsgI    BsiEI     BsmI
    BspHI    BsrDI   BstAPI    BstYI     BtgI     BtsI    CspCI     EaeI
     EarI  Eco57MI    EcoNI     FalI    HaeII    Hin4I   HincII  HindIII
     HpaI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NsiI
    PvuII     SfcI     StyI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BciVI     BclI     BglI     BlpI Bme1580I    BmgBI     BmtI
     BplI    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseYI
  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI     KasI     KpnI
     MfeI     MluI     NaeI     NarI   NgoMIV     NheI     NotI     NruI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI