Restriction map for pF1KB6472
##################################
# NO CUT ENZYMES (  115 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AgeI        AhdI        Alw44I      AlwNI       AocI        
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AvaI        AviII       
AvrII       BanI        BanII       BbeI        BbsI        
BciVI       BclI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BpuAI       BsaI        BsiWI       
BsmBI       BsoBI       Bsp1407I    BspDI       BspHI       
BspLU11I    BsrDI       BsrGI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DrdI        
Eam1105I    Eco81I      EcoO65I     EcoRI       EcoRV       
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MfeI        MluI        MseI        MunI        NarI        
NdeI        NotI        NruI        NspV        PacI        
PaeR7I      PflMI       PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        SwaI        TfiI        Tru9I       Tsp509I     
Van91I      XbaI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   58 enzymes) #
###################################

AccI          217  GTCTAC               GT^[AC][GT]AC
AccIII        383  TCCGGA               T^CCGGA
AflIII        785  ACACGT               A^C[GA][CT]GT
Aor51HI        41  AGCGCT               AGC^GCT
Asp700I       744  GAAGCTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1012  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          245  TGGCCA               TGG^CCA
BamHI         635  GGATCC               G^GATCC
BbrPI         972  CACGTG               CAC^GTG
BbvCI         706  GCTGAGG              CCTCAGC(-5/-2)
BcgI          450  CGACTCAGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          207  CCGGG                CC^[GC]GG
BfaI          703  CTAG                 C^TAG
BglII         956  AGATCT               A^GATCT
BsaAI         972  CACGTG               [CT]AC^GT[GA]
BsaBI         156  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         383  TCCGGA               [AT]^CCGG[AT]
BseAI         383  TCCGGA               T^CCGGA
BsiEI          35  CGGCCG               CG[GA][CT]^CG
BsmAI         559  GAGAC                GTCTC(1/5)
BsmFI         845  GTCCC                GGGAC(10/14)
BsmI          193  GAATGC               GAATGC(1/-1)
BspEI         383  TCCGGA               T^CCGGA
BsrFI         197  GCCGGC               [GA]^CCGG[CT]
BsrI          234  CCAGT                ACTGG(1/-1)
BssSI         169  CACGAG               CACGAG(-5/-1)
BstXI         168  CCACGAGGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        197  GCCGGC               [GA]^CCGG[CT]
DraIII        491  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI           35  CGGCCG               C^GGCCG
EclXI          35  CGGCCG               C^GGCCG
Eco47III       41  AGCGCT               AGC^GCT
Eco52I         35  CGGCCG               C^GGCCG
EcoT22I       730  ATGCAT               ATGCA^T
MaeI          703  CTAG                 C^TAG
MamI          156  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         245  TGGCCA               TGG^CCA
MroI          383  TCCGGA               T^CCGGA
MscI          245  TGGCCA               TGG^CCA
MslI          169  CACGAGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          197  GCCGGC               GCC^GGC
NciI          207  CCGGG                CC^[GC]GG
NgoMIV        197  GCCGGC               G^CCGGC
NheI          702  GCTAGC               G^CTAGC
NsiI          730  ATGCAT               ATGCA^T
NspI          728  GCATGC               [GA]CATG^[CT]
PflFI        1012  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         972  CACGTG               CAC^GTG
PmlI          972  CACGTG               CAC^GTG
PstI          271  CTGCAG               CTGCA^G
PvuII         455  CAGCTG               CAG^CTG
SapI          135  GAAGAGC              GCTCTTC(1/4)
SfaNI         758  GCATC                GCATC(5/9)
SphI          728  GCATGC               GCATG^C
StuI          413  AGGCCT               AGG^CCT
Tth111I      1012  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          545  CCACGGGGATCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          744  GAAGCTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccII          92  CGCG                 CG^CG
              535  CGCG                
AfaI          204  GTAC                 GT^AC
              870  GTAC                
AspHI         138  GAGCAC               G[AT]GC[AT]^C
              355  GTGCTC              
BmyI          138  GAGCAC               G[AGT]GC[ACT]^C
              355  GTGCTC              
BpmI          294  CTCCAG               CTGGAG(16/14)
              478  CTGGAG              
BsiHKAI       138  GAGCAC               G[AT]GC[AT]^C
              355  GTGCTC              
Bsp1286I      138  GAGCAC               G[AGT]GC[ACT]^C
              355  GTGCTC              
BspMI          65  ACCTGC               ACCTGC(4/8)
              269  ACCTGC              
BstAPI        348  GCAAGGCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              516  GCAAGATGTGC         
BstNI         311  CCAGG                CC^[AT]GG
              477  CCTGG               
BstUI          92  CGCG                 CG^CG
              535  CGCG                
BstYI         635  GGATCC               [GA]^GATC[CT]
              956  AGATCT              
EcoNI          66  CCTGCGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              819  CCTGTTTGAGG         
EcoRII        311  CCAGG                ^CC[AT]GG
              477  CCTGG               
HinfI         127  GAGTC                G^A[ACGT]TC
              451  GACTC               
MflI          635  GGATCC               [GA]^GATC[CT]
              956  AGATCT              
MspA1I        455  CAGCTG               C[AC]G^C[GT]G
              613  CAGCGG              
MvaI          311  CCAGG                CC^[AT]GG
              477  CCTGG               
MvnI           92  CGCG                 CG^CG
              535  CGCG                
NcoI          260  CCATGG               C^CATGG
              402  CCATGG              
PleI          451  GACTC                GAGTC(4/5)
              127  GAGTC               
RsaI          204  GTAC                 GT^AC
              870  GTAC                
SfcI          219  CTACAG               C^T[GA][CT]AG
              271  CTGCAG              
SmlI          429  CTCAAG               C^T[CT][GA]AG
              681  CTTGAG              
TspRI         326  CACTG                CA[GC]TG[ACGT][ACGT]^
              640  CACTG               
XhoII         635  GGATCC               [GA]^GATC[CT]
              956  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AciI        AcyI        AluI        AlwI        AvaII       
BbvI        BsaHI       BsaJI       BseRI       BsgI        
BsiYI       BslI        BsrBI       BssKI       BstF5I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MnlI        
MspI        MwoI        NdeII       NlaIII      NlaIV       
PpuMI       Sau3AI      Sau96I      ScrFI       StyI        
TaqI        TseI        Tsp45I      TthHB8I     
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