Restriction map for pF1KB6472
 (Linear) (Six-base) MAPSORT of: KIBB6472.seq  Check: 1391  from: 1  to: 1065



KIBB6472.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AccI GT'mk_AC

Cuts at:      0     218    1065 
   Size:        218     847
 
AfeI AGC'GCT

Cuts at:      0      43    1065 
   Size:         43    1022
 
AflIII A'CryG_T

Cuts at:      0     785    1065 
   Size:        785     280
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     338     348    1065 
   Size:        338      10     717

  Fragments arranged by size: 

                 717     338      10
 
BamHI G'GATC_C

Cuts at:      0     635    1065 
   Size:        635     430
 
BbvCI CC'TCA_GC

Cuts at:      0     707    1065 
   Size:        707     358
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     439     473    1065 
   Size:        439      34     592

  Fragments arranged by size: 

                 592     439      34
 
BfrBI ATG'CAT

Cuts at:      0     732    1065 
   Size:        732     333
 
BglII A'GATC_T

Cuts at:      0     956    1065 
   Size:        956     109
 
BmgBI CAC'GTC

Cuts at:      0     788    1065 
   Size:        788     277
 
BmtI G_CTAG'C

Cuts at:      0     706    1065 
   Size:        706     359
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     279     499    1065 
   Size:        279     220     566

  Fragments arranged by size: 

                 566     279     220
 
Bpu10I CC'TnA_GC

Cuts at:      0     707    1065 
   Size:        707     358
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     414     702    1065 
   Size:        414     288     363

  Fragments arranged by size: 

                 414     363     288
 
BsaAI yAC'GTr

Cuts at:      0     974    1065 
   Size:        974      91
 
BsaBI GATnn'nnATC

Cuts at:      0     160    1065 
   Size:        160     905
 
BsaHI Gr'CG_yC

Cuts at:      0     308     377     653    1013    1065 
   Size:        308      69     276     360      52

  Fragments arranged by size: 

                 360     308     276      69      52
 
BsaWI w'CCGG_w

Cuts at:      0     383    1065 
   Size:        383     682
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      88     118    1065 
   Size:         88      30     947

  Fragments arranged by size: 

                 947      88      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     202     358     730     841    1065 
   Size:        202     156     372     111     224

  Fragments arranged by size: 

                 372     224     202     156     111
 
BseYI C'CCAG_C

Cuts at:      0     121    1065 
   Size:        121     944
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     318     430    1018    1065 
   Size:        318     112     588      47

  Fragments arranged by size: 

                 588     318     112      47
 
BsiEI CG_ry'CG

Cuts at:      0      38    1065 
   Size:         38    1027
 
BsiHKAI G_wGCw'C

Cuts at:      0     142     359    1065 
   Size:        142     217     706

  Fragments arranged by size: 

                 706     217     142
 
BsmI GAATG_Cn'

Cuts at:      0     199    1065 
   Size:        199     866
 
Bsp1286I G_dGCh'C

Cuts at:      0     142     359    1065 
   Size:        142     217     706

  Fragments arranged by size: 

                 706     217     142
 
BspEI T'CCGG_A

Cuts at:      0     383    1065 
   Size:        383     682
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      74     278    1065 
   Size:         74     204     787

  Fragments arranged by size: 

                 787     204      74
 
BsrBI CCG'CTC

Cuts at:      0     301     428     624    1065 
   Size:        301     127     196     441

  Fragments arranged by size: 

                 441     301     196     127
 
BsrFI r'CCGG_y

Cuts at:      0     197    1065 
   Size:        197     868
 
BssSI C'ACGA_G

Cuts at:      0     169    1065 
   Size:        169     896
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     354     522    1065 
   Size:        354     168     543

  Fragments arranged by size: 

                 543     354     168
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     175    1065 
   Size:        175     890
 
BstYI r'GATC_y

Cuts at:      0     635     956    1065 
   Size:        635     321     109

  Fragments arranged by size: 

                 635     321     109
 
BtgI C'CryG_G

Cuts at:      0     260     402     545    1065 
   Size:        260     142     143     520

  Fragments arranged by size: 

                 520     260     143     142
 
BtsI GCAGTG_nn'

Cuts at:      0     639    1065 
   Size:        639     426
 
DraIII CAC_nnn'GTG

Cuts at:      0     496    1065 
   Size:        496     569
 
EaeI y'GGCC_r

Cuts at:      0      35     245     423    1065 
   Size:         35     210     178     642

  Fragments arranged by size: 

                 642     210     178      35
 
EagI C'GGCC_G

Cuts at:      0      35    1065 
   Size:         35    1030
 
EarI CTCTTCn'nnn_

Cuts at:      0     130     805    1065 
   Size:        130     675     260

  Fragments arranged by size: 

                 675     260     130
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     279     499    1065 
   Size:        279     220     566

  Fragments arranged by size: 

                 566     279     220
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      70     823    1065 
   Size:         70     753     242

  Fragments arranged by size: 

                 753     242      70
 
EcoO109I rG'GnC_Cy

Cuts at:      0     579     816     927    1065 
   Size:        579     237     111     138

  Fragments arranged by size: 

                 579     237     138     111
 
HaeII r_GCGC'y

Cuts at:      0      20      45     689    1065 
   Size:         20      25     644     376

  Fragments arranged by size: 

                 644     376      25      20
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     166     198     355     387    1065 
   Size:        166      32     157      32     678

  Fragments arranged by size: 

                 678     166     157      32      32
 
MscI TGG'CCA

Cuts at:      0     247    1065 
   Size:        247     818
 
MslI CAynn'nnrTG

Cuts at:      0     173    1065 
   Size:        173     892
 
MspA1I CmG'CkG

Cuts at:      0     457     615    1065 
   Size:        457     158     450

  Fragments arranged by size: 

                 457     450     158
 
NaeI GCC'GGC

Cuts at:      0     199    1065 
   Size:        199     866
 
NcoI C'CATG_G

Cuts at:      0     260     402    1065 
   Size:        260     142     663

  Fragments arranged by size: 

                 663     260     142
 
NgoMIV G'CCGG_C

Cuts at:      0     197    1065 
   Size:        197     868
 
NheI G'CTAG_C

Cuts at:      0     702    1065 
   Size:        702     363
 
NsiI A_TGCA'T

Cuts at:      0     734    1065 
   Size:        734     331
 
NspI r_CATG'y

Cuts at:      0     732    1065 
   Size:        732     333
 
PmlI CAC'GTG

Cuts at:      0     974    1065 
   Size:        974      91
 
PpuMI rG'GwC_Cy

Cuts at:      0     579     816     927    1065 
   Size:        579     237     111     138

  Fragments arranged by size: 

                 579     237     138     111
 
PstI C_TGCA'G

Cuts at:      0     275    1065 
   Size:        275     790
 
PvuII CAG'CTG

Cuts at:      0     457    1065 
   Size:        457     608
 
SapI GCTCTTCn'nnn_

Cuts at:      0     130    1065 
   Size:        130     935
 
SfcI C'TryA_G

Cuts at:      0     219     271    1065 
   Size:        219      52     794

  Fragments arranged by size: 

                 794     219      52
 
SmlI C'TyrA_G

Cuts at:      0     429     681    1065 
   Size:        429     252     384

  Fragments arranged by size: 

                 429     384     252
 
SphI G_CATG'C

Cuts at:      0     732    1065 
   Size:        732     333
 
StuI AGG'CCT

Cuts at:      0     415    1065 
   Size:        415     650
 
StyI C'CwwG_G

Cuts at:      0     260     402     950    1065 
   Size:        260     142     548     115

  Fragments arranged by size: 

                 548     260     142     115
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     106     595    1065 
   Size:        106     489     470

  Fragments arranged by size: 

                 489     470     106
 
TatI w'GTAC_w

Cuts at:      0     869    1065 
   Size:        869     196
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     828     860    1065 
   Size:        828      32     205

  Fragments arranged by size: 

                 828     205      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1015    1065 
   Size:       1015      50
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     552    1065 
   Size:        552     513
 
XmnI GAAnn'nnTTC

Cuts at:      0     748    1065 
   Size:        748     317
 

 Enzymes that do cut:

     AccI     AfeI   AflIII     AlfI    BamHI    BbvCI     BcgI    BfrBI
    BglII    BmgBI     BmtI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI    BssSI   BstAPI
    BstXI    BstYI     BtgI     BtsI   DraIII     EaeI     EagI     EarI
  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I     MscI     MslI   MspA1I
     NaeI     NcoI   NgoMIV     NheI     NsiI     NspI     PmlI    PpuMI
     PstI    PvuII     SapI     SfcI     SmlI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII     AgeI     AhdI
     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI     BbsI
    BciVI     BclI     BglI     BlpI Bme1580I     BmrI     BplI     BsaI
    BsiWI    BsmBI    BspHI    BsrDI    BsrGI   BssHII    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI     DrdI     EciI
  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NarI
     NdeI     NotI     NruI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SspI     SwaI     XbaI     XhoI     XmaI     ZraI
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