Restriction map for pF1KB6507
##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AvrII       
BalI        BbsI        BcgI        BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaI        BsgI        BsiWI       
Bsp1407I    BspDI       BspHI       BspLU11I    BsrDI       
BsrGI       BsrI        BssHII      BssSI       Bst1107I    
BstBI       BstXI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        Eam1105I    Eco47III    
Eco81I      EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HindIII     HpaI        KpnI        KspI        
MaeI        MfeI        MluI        MluNI       MscI        
MseI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        SfiI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Tru9I       
Tsp509I     XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccI          235  GTCTAC               GT^[AC][GT]AC
AflIII        985  ACGTGT               A^C[GA][CT]GT
AspI          706  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         176  TGCGCA               TGC^GCA
BamHI         647  GGATCC               G^GATCC
BanI          190  GGCGCC               G^G[CT][GA]CC
BanII         115  GAGCTC               G[GA]GC[CT]^C
BbeI          190  GGCGCC               GGCGC^C
BbrPI         984  CACGTG               CAC^GTG
BbvCI         693  CCTCAGC              CCTCAGC(-5/-2)
BsaAI         984  CACGTG               [CT]AC^GT[GA]
BsaBI         174  GATGCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI         283  GAGAC                GTCTC(1/5)
BsmBI         283  GAGACG               CGTCTC(1/5)
BsmI          427  GAATGC               GAATGC(1/-1)
BstAPI        528  GCAGGACGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        360  GGTGACC              G^GT[ACGT]ACC
BstPI         360  GGTGACC              G^GT[ACGT]ACC
DraIII        859  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI            3  GACTCTGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         1025  CGGCCG               [CT]^GGCC[GA]
EagI         1025  CGGCCG               C^GGCCG
EclXI        1025  CGGCCG               C^GGCCG
Eco52I       1025  CGGCCG               C^GGCCG
EcoO65I       360  GGTGACC              G^GT[ACGT]ACC
FspI          176  TGCGCA               TGC^GCA
HaeII         190  GGCGCC               [GA]GCGC^[CT]
HincII        490  GTTGAC               GT[CT]^[GA]AC
HindII        490  GTTGAC               GT[CT]^[GA]AC
KasI          190  GGCGCC               G^GCGCC
MamI          174  GATGCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI          252  CACCGCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          193  GCCGGC               GCC^GGC
NarI          190  GGCGCC               GG^CGCC
NcoI          278  CCATGG               C^CATGG
NgoMIV        193  GCCGGC               G^CCGGC
PflFI         706  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         708  CCAAGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         984  CACGTG               CAC^GTG
PmlI          984  CACGTG               CAC^GTG
PstI         1045  CTGCAG               CTGCA^G
PvuII          82  CAGCTG               CAG^CTG
SacI          115  GAGCTC               GAGCT^C
SapI          153  GAAGAGC              GCTCTTC(1/4)
ScaI          473  AGTACT               AGT^ACT
SfcI         1045  CTGCAG               C^T[GA][CT]AG
SfoI          190  GGCGCC               GGC^GCC
SmaI          414  CCCGGG               CCC^GGG
Tth111I       706  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        708  CCAAGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         414  CCCGGG               C^CCGGG
XmaI          414  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccIII        602  TCCGGA               T^CCGGA
              764  TCCGGA              
Asp700I       243  GAACATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              930  GAAGATGTTC          
AspHI         115  GAGCTC               G[AT]GC[AT]^C
              156  GAGCAC              
AvaI          414  CCCGGG               C^[CT]CG[GA]G
              877  CCCGAG              
BglI          256  GCCATGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              904  GCCCAGGCGGC         
BseAI         602  TCCGGA               T^CCGGA
              764  TCCGGA              
BsiHKAI       115  GAGCTC               G[AT]GC[AT]^C
              156  GAGCAC              
BsoBI         414  CCCGGG               C^[CT]CG[GA]G
              877  CCCGAG              
BspEI         602  TCCGGA               T^CCGGA
              764  TCCGGA              
BspMI         515  ACCTGC               ACCTGC(4/8)
              830  ACCTGC              
BsrBI         147  GAGCGG               CCGCTC(-3/-3)
              634  GAGCGG              
BsrFI         193  GCCGGC               [GA]^CCGG[CT]
              559  ACCGGC              
Cfr10I        193  GCCGGC               [GA]^CCGG[CT]
              559  ACCGGC              
DdeI           31  CTGAG                C^T[ACGT]AG
              694  CTCAG               
DraII         410  AGGACCC              [GA]G^G[ACGT]CC[CT]
              827  AGGACCT             
DsaI          186  CCACGG               C^C[GA][CT]GG
              278  CCATGG              
EarI          811  CTCTTC               CTCTTC(1/4)
              153  GAAGAG              
EcoNI         402  CCTGTGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              831  CCTGCTGGAGG         
EcoO109I      410  AGGACCC              [GA]G^G[ACGT]CC[CT]
              827  AGGACCT             
EcoT14I       278  CCATGG               C^C[AT][AT]GG
              506  CCTTGG              
Ksp632I       811  CTCTTC               CTCTTC(1/4)
              153  GAAGAG              
MaeIII        361  GTGAC                ^GT[ACGT]AC
              667  GTGAC               
MroI          602  TCCGGA               T^CCGGA
              764  TCCGGA              
PpuMI         410  AGGACCC              [GA]G^G[AT]CC[CT]
              827  AGGACCT             
SexAI         860  ACCTGGT              A^CC[AT]GGT
             1070  ACCTGGT             
StyI          278  CCATGG               C^C[AT][AT]GG
              506  CCTTGG              
Tsp45I        361  GTGAC                ^GT[GC]AC
              667  GTGAC               
TspRI         387  CAGTG                CA[GC]TG[ACGT][ACGT]^
              652  CACTG               
XmnI          243  GAACATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              930  GAAGATGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AvaII       BbvI        BcnI        BmyI        
BpmI        BsaHI       BsaJI       BsaWI       BseRI       
BsiEI       BsiYI       BslI        BsmFI       Bsp1286I    
BssKI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DpnII       EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MboI        
MboII       MflI        MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SmlI        TaqI        
TseI        TthHB8I     XhoII       
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