Restriction map for pF1KB6507
 (Linear) (Six-base) MAPSORT of: KIBB6507.seq  Check: 1293  from: 1  to: 1077



KIBB6507.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AccI GT'mk_AC

Cuts at:      0     236    1077 
   Size:        236     841
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     949    1077 
   Size:        949     128
 
AflIII A'CryG_T

Cuts at:      0     985    1077 
   Size:        985      92
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     655     687    1077 
   Size:        655      32     390

  Fragments arranged by size: 

                 655     390      32
 
AvaI C'yCGr_G

Cuts at:      0     414     877    1077 
   Size:        414     463     200

  Fragments arranged by size: 

                 463     414     200
 
BamHI G'GATC_C

Cuts at:      0     647    1077 
   Size:        647     430
 
BanI G'GyrC_C

Cuts at:      0     190    1077 
   Size:        190     887
 
BanII G_rGCy'C

Cuts at:      0     119    1077 
   Size:        119     958
 
BbeI G_GCGC'C

Cuts at:      0     194    1077 
   Size:        194     883
 
BbvCI CC'TCA_GC

Cuts at:      0     694    1077 
   Size:        694     383
 
BglI GCCn_nnn'nGGC

Cuts at:      0     262     910    1077 
   Size:        262     648     167

  Fragments arranged by size: 

                 648     262     167
 
Bme1580I G_kGCm'C

Cuts at:      0     554    1077 
   Size:        554     523
 
BmgBI CAC'GTC

Cuts at:      0     351     534    1077 
   Size:        351     183     543

  Fragments arranged by size: 

                 543     351     183
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     607     856    1077 
   Size:         28     579     249     221

  Fragments arranged by size: 

                 579     249     221      28
 
Bpu10I CC'TnA_GC

Cuts at:      0      31     694    1077 
   Size:         31     663     383

  Fragments arranged by size: 

                 663     383      31
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     274    1042    1077 
   Size:        103     171     768      35

  Fragments arranged by size: 

                 768     171     103      35
 
BsaAI yAC'GTr

Cuts at:      0     986    1077 
   Size:        986      91
 
BsaBI GATnn'nnATC

Cuts at:      0     178    1077 
   Size:        178     899
 
BsaHI Gr'CG_yC

Cuts at:      0     104     191     902    1077 
   Size:        104      87     711     175

  Fragments arranged by size: 

                 711     175     104      87
 
BsaWI w'CCGG_w

Cuts at:      0     602     737     764    1077 
   Size:        602     135      27     313

  Fragments arranged by size: 

                 602     313     135      27
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     442     472    1077 
   Size:        442      30     605

  Fragments arranged by size: 

                 605     442      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     220     760     789    1077 
   Size:        220     540      29     288

  Fragments arranged by size: 

                 540     288     220      29
 
BseYI C'CCAG_C

Cuts at:      0     524     893    1077 
   Size:        524     369     184

  Fragments arranged by size: 

                 524     369     184
 
BsiEI CG_ry'CG

Cuts at:      0     438     631    1028    1077 
   Size:        438     193     397      49

  Fragments arranged by size: 

                 438     397     193      49
 
BsiHKAI G_wGCw'C

Cuts at:      0     119     160    1077 
   Size:        119      41     917

  Fragments arranged by size: 

                 917     119      41
 
BsmI GAATG_Cn'

Cuts at:      0     433    1077 
   Size:        433     644
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     277    1077 
   Size:        277     800
 
Bsp1286I G_dGCh'C

Cuts at:      0     119     160     554    1077 
   Size:        119      41     394     523

  Fragments arranged by size: 

                 523     394     119      41
 
BspEI T'CCGG_A

Cuts at:      0     602     764    1077 
   Size:        602     162     313

  Fragments arranged by size: 

                 602     313     162
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     524     839    1077 
   Size:        524     315     238

  Fragments arranged by size: 

                 524     315     238
 
BsrBI CCG'CTC

Cuts at:      0     149     636    1077 
   Size:        149     487     441

  Fragments arranged by size: 

                 487     441     149
 
BsrFI r'CCGG_y

Cuts at:      0     193     559    1077 
   Size:        193     366     518

  Fragments arranged by size: 

                 518     366     193
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     534    1077 
   Size:        534     543
 
BstEII G'GTnAC_C

Cuts at:      0     360    1077 
   Size:        360     717
 
BstYI r'GATC_y

Cuts at:      0     293     647     845    1077 
   Size:        293     354     198     232

  Fragments arranged by size: 

                 354     293     232     198
 
BtgI C'CryG_G

Cuts at:      0     186     278    1077 
   Size:        186      92     799

  Fragments arranged by size: 

                 799     186      92
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      50     483    1077 
   Size:         50     433     594

  Fragments arranged by size: 

                 594     433      50
 
BtsI GCAGTG_nn'

Cuts at:      0     651    1077 
   Size:        651     426
 
DraIII CAC_nnn'GTG

Cuts at:      0     864    1077 
   Size:        864     213
 
DrdI GACnn_nn'nnGTC

Cuts at:      0       9    1077 
   Size:          9    1068
 
EaeI y'GGCC_r

Cuts at:      0    1025    1077 
   Size:       1025      52
 
EagI C'GGCC_G

Cuts at:      0    1025    1077 
   Size:       1025      52
 
EarI CTCTTCn'nnn_

Cuts at:      0     148     817    1077 
   Size:        148     669     260

  Fragments arranged by size: 

                 669     260     148
 
EcoICRI GAG'CTC

Cuts at:      0     117    1077 
   Size:        117     960
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     607     856     949    1077 
   Size:         28     579     249      93     128

  Fragments arranged by size: 

                 579     249     128      93      28
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     406     835    1077 
   Size:        406     429     242

  Fragments arranged by size: 

                 429     406     242
 
EcoO109I rG'GnC_Cy

Cuts at:      0     411     828    1077 
   Size:        411     417     249

  Fragments arranged by size: 

                 417     411     249
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     237    1077 
   Size:        237     840
 
FspI TGC'GCA

Cuts at:      0     178    1077 
   Size:        178     899
 
FspAI rTGC'GCAy

Cuts at:      0     178    1077 
   Size:        178     899
 
HaeII r_GCGC'y

Cuts at:      0     194    1077 
   Size:        194     883
 
HincII GTy'rAC

Cuts at:      0     492    1077 
   Size:        492     585
 
KasI G'GCGC_C

Cuts at:      0     190    1077 
   Size:        190     887
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     485     611     704    1077 
   Size:        485     126      93     373

  Fragments arranged by size: 

                 485     373     126      93
 
MslI CAynn'nnrTG

Cuts at:      0     256    1077 
   Size:        256     821
 
MspA1I CmG'CkG

Cuts at:      0      84     627     897    1077 
   Size:         84     543     270     180

  Fragments arranged by size: 

                 543     270     180      84
 
NaeI GCC'GGC

Cuts at:      0     195    1077 
   Size:        195     882
 
NarI GG'CG_CC

Cuts at:      0     191    1077 
   Size:        191     886
 
NcoI C'CATG_G

Cuts at:      0     278    1077 
   Size:        278     799
 
NgoMIV G'CCGG_C

Cuts at:      0     193    1077 
   Size:        193     884
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     714    1077 
   Size:        714     363
 
PfoI T'CCnGG_A

Cuts at:      0     338     422    1077 
   Size:        338      84     655

  Fragments arranged by size: 

                 655     338      84
 
PmlI CAC'GTG

Cuts at:      0     986    1077 
   Size:        986      91
 
PpuMI rG'GwC_Cy

Cuts at:      0     411     828    1077 
   Size:        411     417     249

  Fragments arranged by size: 

                 417     411     249
 
PstI C_TGCA'G

Cuts at:      0    1049    1077 
   Size:       1049      28
 
PvuII CAG'CTG

Cuts at:      0      84    1077 
   Size:         84     993
 
SacI G_AGCT'C

Cuts at:      0     119    1077 
   Size:        119     958
 
SapI GCTCTTCn'nnn_

Cuts at:      0     148    1077 
   Size:        148     929
 
ScaI AGT'ACT

Cuts at:      0     475    1077 
   Size:        475     602
 
SexAI A'CCwGG_T

Cuts at:      0     860    1070    1077 
   Size:        860     210       7

  Fragments arranged by size: 

                 860     210       7
 
SfcI C'TryA_G

Cuts at:      0    1045    1077 
   Size:       1045      32
 
SfoI GGC'GCC

Cuts at:      0     192    1077 
   Size:        192     885
 
SmaI CCC'GGG

Cuts at:      0     416    1077 
   Size:        416     661
 
SmlI C'TyrA_G

Cuts at:      0     118     289    1057    1077 
   Size:        118     171     768      20

  Fragments arranged by size: 

                 768     171     118      20
 
StyI C'CwwG_G

Cuts at:      0     278     506    1077 
   Size:        278     228     571

  Fragments arranged by size: 

                 571     278     228
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     499     538    1077 
   Size:        499      39     539

  Fragments arranged by size: 

                 539     499      39
 
TatI w'GTAC_w

Cuts at:      0     473     851    1064    1077 
   Size:        473     378     213      13

  Fragments arranged by size: 

                 473     378     213      13
 
Tth111I GACn'n_nGTC

Cuts at:      0     709    1077 
   Size:        709     368
 
XmaI C'CCGG_G

Cuts at:      0     414    1077 
   Size:        414     663
 
XmnI GAAnn'nnTTC

Cuts at:      0     247     934    1077 
   Size:        247     687     143

  Fragments arranged by size: 

                 687     247     143
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AloI     AvaI    BamHI     BanI    BanII
     BbeI    BbvCI     BglI Bme1580I    BmgBI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI
   BstAPI   BstEII    BstYI     BtgI    BtgZI     BtsI   DraIII     DrdI
     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
     FspI    FspAI    HaeII   HincII     KasI     MmeI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV    PflMI     PfoI     PmlI    PpuMI
     PstI    PvuII     SacI     SapI     ScaI    SexAI     SfcI     SfoI
     SmaI     SmlI     StyI    TaqII     TatI  Tth111I     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI     BbsI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI     BmrI     BmtI     BplI     BsaI     BsgI    BsiWI
    BspHI    BsrDI    BsrGI   BssHII    BssSI    BstBI    BstXI  BstZ17I
   Bsu36I     ClaI    CspCI     DraI     EciI    EcoRI    EcoRV     FseI
    Hin4I  HindIII     HpaI     KpnI     MfeI     MluI     MscI     NdeI
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SbfI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI
     XbaI     XcmI     XhoI     ZraI
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