Restriction map for pF1KE0345
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# NO CUT ENZYMES (  113 enzymes) #
##################################

AatII       AccI        AclI        AcsI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     AspEI       AspI        
AviII       AvrII       BbeI        BbrPI       BbsI        
BciVI       BclI        BfaI        BfrI        BlnI        
BpuAI       BsaAI       BsaBI       BsaHI       BsiWI       
BspDI       BspLU11I    BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
ClaI        DraI        DraIII      Eam1105I    Eco47III    
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        MaeI        MamI        MfeI        MluI        
MseI        MunI        NarI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        SacI        SalI        SapI        SbfI        
ScaI        SexAI       SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        TfiI        Tru9I       Tth111I     
XbaI        XhoI        XmnI        


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# ONE CUT ENZYMES (   46 enzymes) #
###################################

Acc65I       1302  GGTACC               G^GTACC
AccIII       1394  TCCGGA               T^CCGGA
Asp718I      1302  GGTACC               G^GTACC
BamHI        1241  GGATCC               G^GATCC
BglI         1379  GCCAGGACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1070  AGATCT               A^GATCT
BsaI         1582  GAGACC               GGTCTC(1/5)
BseAI        1394  TCCGGA               T^CCGGA
BseRI        1498  GAGGAG               GAGGAG(10/8)
BsiEI         728  CGGCCG               CG[GA][CT]^CG
BsmI          234  GAATGC               GAATGC(1/-1)
BspEI        1394  TCCGGA               T^CCGGA
BspHI         230  TCATGA               T^CATGA
BspMI        1314  ACCTGC               ACCTGC(4/8)
BsrDI         204  CATTGC               GCAATG(2/0)
BsrFI        1699  GCCGGC               [GA]^CCGG[CT]
CfoI          835  GCGC                 GCG^C
Cfr10I       1699  GCCGGC               [GA]^CCGG[CT]
CpoI          544  CGGACCG              CG^G[AT]CCG
DrdI         1384  GACGGCCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          728  CGGCCG               C^GGCCG
EclXI         728  CGGCCG               C^GGCCG
Eco52I        728  CGGCCG               C^GGCCG
HhaI          835  GCGC                 GCG^C
HinP1I        835  GCGC                 G^CGC
HinfI        1675  GACTC                G^A[ACGT]TC
KpnI         1302  GGTACC               GGTAC^C
KspI          131  CCGCGG               CCGC^GG
MroI         1394  TCCGGA               T^CCGGA
NaeI         1699  GCCGGC               GCC^GGC
NgoMIV       1699  GCCGGC               G^CCGGC
NspI          824  ACATGC               [GA]CATG^[CT]
PflMI         240  CCACAGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI         1675  GACTC                GAGTC(4/5)
PstI         1571  CTGCAG               CTGCA^G
RcaI          230  TCATGA               T^CATGA
RsrII         544  CGGACCG              CG^G[AT]CCG
SacII         131  CCGCGG               CCGC^GG
SfcI         1571  CTGCAG               C^T[GA][CT]AG
SfiI         1378  GGCCAGGACGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          896  CCCGGG               CCC^GGG
StuI          677  AGGCCT               AGG^CCT
Tsp509I      1321  AATT                 ^AATT
Van91I        240  CCACAGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         896  CCCGGG               C^CCGGG
XmaI          896  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AlwNI        1519  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1565  CAGTGACTG           
ApaI          847  GGGCCC               GGGCC^C
             1064  GGGCCC              
AspHI         147  GTGCTC               G[AT]GC[AT]^C
              784  GAGCAC              
BalI          659  TGGCCA               TGG^CCA
             1448  TGGCCA              
BcgI          245  GCATGGGCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1663  GCAGCTGCCTCG        
BlpI          772  GCTGAGC              GC^T[ACGT]AGC
              840  GCTCAGC             
BpmI         1677  CTCCAG               CTGGAG(16/14)
             1579  CTGGAG              
Bpu1102I      772  GCTGAGC              GC^T[ACGT]AGC
              840  GCTCAGC             
BsaWI         959  TCCGGT               [AT]^CCGG[AT]
             1394  TCCGGA              
BsgI         1442  CTGCAC               GTGCAG(16/14)
             1510  CTGCAC              
BsiHKAI       147  GTGCTC               G[AT]GC[AT]^C
              784  GAGCAC              
BsmBI         652  GAGACG               CGTCTC(1/5)
              809  GAGACG              
Bsp1407I      110  TGTACA               T^GTACA
              821  TGTACA              
BsrGI         110  TGTACA               T^GTACA
              821  TGTACA              
BsrI         1446  ACTGG                ACTGG(1/-1)
              830  CCAGT               
BstAPI        199  GCAGCCATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1564  GCAGTGACTGC         
CelII         772  GCTGAGC              GC^T[ACGT]AGC
              840  GCTCAGC             
EarI          265  CTCTTC               CTCTTC(1/4)
              466  CTCTTC              
EcoT14I        89  CCAAGG               C^C[AT][AT]GG
             1217  CCAAGG              
HgaI          654  GACGC                GACGC(5/10)
             1609  GCGTC               
Ksp632I       265  CTCTTC               CTCTTC(1/4)
              466  CTCTTC              
MluNI         659  TGGCCA               TGG^CCA
             1448  TGGCCA              
MscI          659  TGGCCA               TGG^CCA
             1448  TGGCCA              
MslI         1536  CACGACAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1620  CATCGCTGTG          
SspBI         110  TGTACA               T^GTACA
              821  TGTACA              
StyI           89  CCAAGG               C^C[AT][AT]GG
             1217  CCAAGG              
XcmI          116  CCAGGGCTGCCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              476  CCAGCATCCAGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AvaI        AvaII       BanI        BanII       BbvCI       
BbvI        BcnI        BmyI        BsaJI       BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BsrBI       BssKI       BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PpuMI       
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       TaqI        TseI        Tsp45I      TspRI       
TthHB8I     XhoII       
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