Restriction map for pF1KE0345
 (Linear) (Six-base) MAPSORT of: KIEE0345.seq  Check: 9457  from: 1  to: 1707



KIEE0345.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1323    1707 
   Size:       1323     384
 
Acc65I G'GTAC_C

Cuts at:      0    1302    1707 
   Size:       1302     405
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1254    1707 
   Size:       1254     453
 
AleI CACnn'nnGTG

Cuts at:      0    1540    1707 
   Size:       1540     167
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1524    1570    1707 
   Size:       1524      46     137

  Fragments arranged by size: 

                1524     137      46
 
ApaI G_GGCC'C

Cuts at:      0     851    1068    1707 
   Size:        851     217     639

  Fragments arranged by size: 

                 851     639     217
 
AvaI C'yCGr_G

Cuts at:      0     896     916    1111    1707 
   Size:        896      20     195     596

  Fragments arranged by size: 

                 896     596     195      20
 
BamHI G'GATC_C

Cuts at:      0    1241    1707 
   Size:       1241     466
 
BanI G'GyrC_C

Cuts at:      0     399     670    1302    1589    1707 
   Size:        399     271     632     287     118

  Fragments arranged by size: 

                 632     399     287     271     118
 
BanII G_rGCy'C

Cuts at:      0     230     462     851     916    1068    1505    1707 
   Size:        230     232     389      65     152     437     202

  Fragments arranged by size: 

                 437     389     232     230     202     152      65
 
BbvCI CC'TCA_GC

Cuts at:      0      81     302     749    1707 
   Size:         81     221     447     958

  Fragments arranged by size: 

                 958     447     221      81
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     234     268    1652    1686    1707 
   Size:        234      34    1384      34      21

  Fragments arranged by size: 

                1384     234      34      34      21
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1385    1707 
   Size:       1385     322
 
BglII A'GATC_T

Cuts at:      0    1070    1707 
   Size:       1070     637
 
BlpI GC'TnA_GC

Cuts at:      0     773     841    1707 
   Size:        773      68     866

  Fragments arranged by size: 

                 866     773      68
 
Bme1580I G_kGCm'C

Cuts at:      0     675     851    1068    1141    1363    1423    1707 
   Size:        675     176     217      73     222      60     284

  Fragments arranged by size: 

                 675     284     222     217     176      73      60
 
BmgBI CAC'GTC

Cuts at:      0     813    1482    1707 
   Size:        813     669     225

  Fragments arranged by size: 

                 813     669     225
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     824    1707 
   Size:        824     883
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1600    1662    1707 
   Size:       1600      62      45

  Fragments arranged by size: 

                1600      62      45
 
Bpu10I CC'TnA_GC

Cuts at:      0      81     302     749    1707 
   Size:         81     221     447     958

  Fragments arranged by size: 

                 958     447     221      81
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1576    1707 
   Size:       1576     131
 
BsaWI w'CCGG_w

Cuts at:      0     959    1394    1707 
   Size:        959     435     313

  Fragments arranged by size: 

                 959     435     313
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     800     830     877     907    1021    1051    1081    1111 
   Size:        800      30      47      30     114      30      30      30

Cuts at:   1111    1198    1228    1707 
   Size:         87      30     479

  Fragments arranged by size: 

                 800     479     114      87      47      30      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1513    1707 
   Size:       1513     194
 
BseYI C'CCAG_C

Cuts at:      0      21     475    1055    1279    1414    1658    1707 
   Size:         21     454     580     224     135     244      49

  Fragments arranged by size: 

                 580     454     244     224     135      49      21
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1427    1495    1707 
   Size:       1427      68     212

  Fragments arranged by size: 

                1427     212      68
 
BsiEI CG_ry'CG

Cuts at:      0     731    1707 
   Size:        731     976
 
BsiHKAI G_wGCw'C

Cuts at:      0     151     788    1707 
   Size:        151     637     919

  Fragments arranged by size: 

                 919     637     151
 
BsmI GAATG_Cn'

Cuts at:      0     240    1707 
   Size:        240    1467
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     646     803    1707 
   Size:        646     157     904

  Fragments arranged by size: 

                 904     646     157
 
Bsp1286I G_dGCh'C

Cuts at:      0     151     230     462     675     788     851     916    1068 
   Size:        151      79     232     213     113      63      65     152

Cuts at:   1068    1141    1363    1423    1505    1707 
   Size:         73     222      60      82     202

  Fragments arranged by size: 

                 232     222     213     202     152     151     113      82
                  79      73      65      63      60
 
BspEI T'CCGG_A

Cuts at:      0    1394    1707 
   Size:       1394     313
 
BspHI T'CATG_A

Cuts at:      0     230    1707 
   Size:        230    1477
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1323    1707 
   Size:       1323     384
 
BsrBI CCG'CTC

Cuts at:      0     840    1226    1347    1707 
   Size:        840     386     121     360

  Fragments arranged by size: 

                 840     386     360     121
 
BsrDI GCAATG_nn'

Cuts at:      0     203    1707 
   Size:        203    1504
 
BsrFI r'CCGG_y

Cuts at:      0    1699    1707 
   Size:       1699       8
 
BsrGI T'GTAC_A

Cuts at:      0     110     821    1707 
   Size:        110     711     886

  Fragments arranged by size: 

                 886     711     110
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     205    1570    1707 
   Size:        205    1365     137

  Fragments arranged by size: 

                1365     205     137
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     442    1005    1446    1707 
   Size:        442     563     441     261

  Fragments arranged by size: 

                 563     442     441     261
 
BstYI r'GATC_y

Cuts at:      0     410    1070    1241    1707 
   Size:        410     660     171     466

  Fragments arranged by size: 

                 660     466     410     171
 
BtgI C'CryG_G

Cuts at:      0      35     125     131     506    1433    1493    1707 
   Size:         35      90       6     375     927      60     214

  Fragments arranged by size: 

                 927     375     214      90      60      35       6
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1605    1707 
   Size:       1605     102
 
BtsI GCAGTG_nn'

Cuts at:      0     151     169    1139    1179    1439    1571    1707 
   Size:        151      18     970      40     260     132     136

  Fragments arranged by size: 

                 970     260     151     136     132      40      18
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1390    1707 
   Size:       1390     317
 
EaeI y'GGCC_r

Cuts at:      0      32     128     659     728    1386    1436    1448    1707 
   Size:         32      96     531      69     658      50      12     259

  Fragments arranged by size: 

                 658     531     259      96      69      50      32      12
            
 
EagI C'GGCC_G

Cuts at:      0     728    1707 
   Size:        728     979
 
EarI CTCTTCn'nnn_

Cuts at:      0     271     472    1707 
   Size:        271     201    1235

  Fragments arranged by size: 

                1235     271     201
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1173    1707 
   Size:       1173     534
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1254    1600    1662    1707 
   Size:       1254     346      62      45

  Fragments arranged by size: 

                1254     346      62      45
 
EcoO109I rG'GnC_Cy

Cuts at:      0     753     927     980     993    1707 
   Size:        753     174      53      13     714

  Fragments arranged by size: 

                 753     714     174      53      13
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     169     201    1258    1290    1707 
   Size:        169      32    1057      32     417

  Fragments arranged by size: 

                1057     417     169      32      32
 
KpnI G_GTAC'C

Cuts at:      0    1306    1707 
   Size:       1306     401
 
MscI TGG'CCA

Cuts at:      0     661    1450    1707 
   Size:        661     789     257

  Fragments arranged by size: 

                 789     661     257
 
MslI CAynn'nnrTG

Cuts at:      0    1540    1624    1707 
   Size:       1540      84      83

  Fragments arranged by size: 

                1540      84      83
 
MspA1I CmG'CkG

Cuts at:      0     133     438     486     636     748     792     845    1238 
   Size:        133     305      48     150     112      44      53     393

Cuts at:   1238    1666    1707 
   Size:        428      41

  Fragments arranged by size: 

                 428     393     305     150     133     112      53      48
                  44      41
 
NaeI GCC'GGC

Cuts at:      0    1701    1707 
   Size:       1701       6
 
NgoMIV G'CCGG_C

Cuts at:      0    1699    1707 
   Size:       1699       8
 
NspI r_CATG'y

Cuts at:      0     828    1707 
   Size:        828     879
 
PasI CC'CwG_GG

Cuts at:      0     455    1707 
   Size:        455    1252
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     246    1707 
   Size:        246    1461
 
PfoI T'CCnGG_A

Cuts at:      0    1678    1707 
   Size:       1678      29
 
PpuMI rG'GwC_Cy

Cuts at:      0     753     980     993    1707 
   Size:        753     227      13     714

  Fragments arranged by size: 

                 753     714     227      13
 
PspOMI G'GGCC_C

Cuts at:      0     847    1064    1707 
   Size:        847     217     643

  Fragments arranged by size: 

                 847     643     217
 
PstI C_TGCA'G

Cuts at:      0    1575    1707 
   Size:       1575     132
 
PvuII CAG'CTG

Cuts at:      0     486     636     748    1666    1707 
   Size:        486     150     112     918      41

  Fragments arranged by size: 

                 918     486     150     112      41
 
RsrII CG'GwC_CG

Cuts at:      0     545    1707 
   Size:        545    1162
 
SacII CC_GC'GG

Cuts at:      0     134    1707 
   Size:        134    1573
 
SanDI GG'GwC_CC

Cuts at:      0     993    1707 
   Size:        993     714
 
SfcI C'TryA_G

Cuts at:      0    1571    1707 
   Size:       1571     136
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1385    1707 
   Size:       1385     322
 
SmaI CCC'GGG

Cuts at:      0     898    1707 
   Size:        898     809
 
StuI AGG'CCT

Cuts at:      0     679    1707 
   Size:        679    1028
 
StyI C'CwwG_G

Cuts at:      0      89    1217    1707 
   Size:         89    1128     490

  Fragments arranged by size: 

                1128     490      89
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1615    1707 
   Size:       1615      92
 
TatI w'GTAC_w

Cuts at:      0     110     821    1707 
   Size:        110     711     886

  Fragments arranged by size: 

                 886     711     110
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     800     832    1081    1113    1707 
   Size:        800      32     249      32     594

  Fragments arranged by size: 

                 800     594     249      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     123     483    1707 
   Size:        123     360    1224

  Fragments arranged by size: 

                1224     360     123
 
XmaI C'CCGG_G

Cuts at:      0     896    1707 
   Size:        896     811
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AleI    AlwNI     ApaI     AvaI    BamHI
     BanI    BanII    BbvCI     BcgI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BpmI   Bpu10I     BsaI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI    BstXI
    BstYI     BtgI    BtgZI     BtsI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI EcoO109I    Hin4I     KpnI     MscI     MslI   MspA1I
     NaeI   NgoMIV     NspI     PasI    PflMI     PfoI    PpuMI   PspOMI
     PstI    PvuII    RsrII    SacII    SanDI     SfcI     SfiI     SmaI
     StuI     StyI    TaqII     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI     BbsI    BciVI     BclI    BfrBI     BmtI     BplI
    BpuEI    BsaAI    BsaBI    BsaHI    BsiWI   BssHII    BssSI    BstBI
   BstEII  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     MfeI     MluI     MmeI     NarI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfoI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
  Tth111I     XbaI     XhoI     XmnI     ZraI
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