Restriction map for pF1KB7694
##################################
# NO CUT ENZYMES (  139 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Aor51HI     ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BalI        BanI        BbeI        
BbrPI       BcgI        BciVI       BclI        BfaI        
BfrI        BlnI        BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
ClaI        CpoI        DraI        DrdI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        Hin1I       HincII      HindII      
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MaeIII      MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MunI        NarI        NcoI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       Tsp45I      Tth111I     XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccII         337  CGCG                 CG^CG
Alw44I        771  GTGCAC               G^TGCAC
AocI          112  CCTGAGG              CC^T[ACGT]AGG
ApaLI         771  GTGCAC               G^TGCAC
AvaI          147  CCCGAG               C^[CT]CG[GA]G
BbsI          408  GAAGAC               GAAGAC(2/6)
BbvCI         196  GCTGAGG              CCTCAGC(-5/-2)
BcnI          527  CCGGG                CC^[GC]GG
BglI          280  GCCGGCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         366  AGATCT               A^GATCT
BpuAI         408  GAAGAC               GAAGAC(2/6)
BseRI         347  CTCCTC               GAGGAG(10/8)
BsgI          449  CTGCAC               GTGCAG(16/14)
BsmAI         400  GAGAC                GTCTC(1/5)
BsoBI         147  CCCGAG               C^[CT]CG[GA]G
BstUI         337  CGCG                 CG^CG
BstXI         389  CCAGCTTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        112  CCTGAGG              CC^T[ACGT]AGG
CfoI          738  GCGC                 GCG^C
DraIII        865  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          877  CGGCCA               [CT]^GGCC[GA]
Eco81I        112  CCTGAGG              CC^T[ACGT]AGG
EcoNI         182  CCTACTACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I      1092  CCAAGG               C^C[AT][AT]GG
HaeII         737  AGCGCC               [GA]GCGC^[CT]
HgaI          193  GACGC                GACGC(5/10)
HhaI          738  GCGC                 GCG^C
HinP1I        738  GCGC                 G^CGC
HindIII       565  AAGCTT               A^AGCTT
MslI         1212  CACTACTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          337  CGCG                 CG^CG
NaeI          280  GCCGGC               GCC^GGC
NciI          527  CCGGG                CC^[GC]GG
NdeI          275  CATATG               CA^TATG
NgoMIV        280  GCCGGC               G^CCGGC
PstI          164  CTGCAG               CTGCA^G
SexAI         866  ACCAGGT              A^CC[AT]GGT
StyI         1092  CCAAGG               C^C[AT][AT]GG
TaqI          912  TCGA                 T^CGA
TfiI           85  GAATC                G^A[AT]TC
TthHB8I       912  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AlwNI        1073  CAGGTACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1118  CAGGGCCTG           
AspHI         150  GAGCAC               G[AT]GC[AT]^C
              771  GTGCAC              
BlpI          420  GCTGAGC              GC^T[ACGT]AGC
             1230  GCTCAGC             
BpmI          954  CTCCAG               CTGGAG(16/14)
              397  CTGGAG              
Bpu1102I      420  GCTGAGC              GC^T[ACGT]AGC
             1230  GCTCAGC             
BsiHKAI       150  GAGCAC               G[AT]GC[AT]^C
              771  GTGCAC              
BspMI         447  ACCTGC               ACCTGC(4/8)
              760  GCAGGT              
BsrBI        1228  CCGCTC               CCGCTC(-3/-3)
              415  GAGCGG              
BsrDI         236  CATTGC               GCAATG(2/0)
              836  GCAATG              
BsrFI         280  GCCGGC               [GA]^CCGG[CT]
              875  ACCGGC              
CelII         420  GCTGAGC              GC^T[ACGT]AGC
             1230  GCTCAGC             
Cfr10I        280  GCCGGC               [GA]^CCGG[CT]
              875  ACCGGC              
DsaI          359  CCGTGG               C^C[GA][CT]GG
             1169  CCACGG              
FauI          335  CCCGC                CCCGC(4/6)
             1028  GCGGG               
PleI          343  GACTC                GAGTC(4/5)
              909  GACTC               
PvuII         167  CAGCTG               CAG^CTG
              989  CAGCTG              
SfaNI         124  GATGC                GCATC(5/9)
              925  GCATC               
Tsp509I       541  AATT                 ^AATT
             1033  AATT                
XcmI          274  CCATATGCCGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1208  CCAGCACTACTGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        ApaI        
AvaII       BamHI       BanII       BbvI        BmyI        
BsaJI       BsiYI       BslI        BsmFI       Bsp1286I    
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        MboI        MboII       MflI        MnlI        
MspA1I      MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PflMI       PpuMI       RsaI        
Sau3AI      Sau96I      ScrFI       SfcI        StuI        
TseI        TspRI       Van91I      XhoII       
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