Restriction map for pF1KB7772
##################################
# NO CUT ENZYMES (  116 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BbeI        
BbrPI       BbsI        BbvCI       BciVI       BclI        
BfrI        BlnI        BpuAI       BsaAI       BsaWI       
BseAI       BseRI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BspMI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstZ17I     
ClaI        CpoI        DraI        DraIII      DrdI        
Eam1105I    Eco47III    EcoRV       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MroI        MseI        MslI        MspA1I      
MunI        NarI        NheI        NotI        NruI        
NsiI        NspI        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tru9I       Tth111I     XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AatII        1138  GACGTC               GACGT^C
AccII          39  CGCG                 CG^CG
AcsI           18  GAATTC               [GA]^AATT[CT]
ApoI           18  GAATTC               [GA]^AATT[CT]
Asp700I       776  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI        1357  GTGCTC               G[AT]GC[AT]^C
AvaI          142  CCCGAG               C^[CT]CG[GA]G
BalI         1223  TGGCCA               TGG^CCA
BamHI           3  GGATCC               G^GATCC
BanI          673  GGTGCC               G^G[CT][GA]CC
BcgI          197  CGATCCTGTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI          437  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      437  GCTCAGC              GC^T[ACGT]AGC
BsaBI        1405  GATCTAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          967  GGTCTC               GGTCTC(1/5)
BsiEI         227  CGGCCG               CG[GA][CT]^CG
BsiHKAI      1357  GTGCTC               G[AT]GC[AT]^C
BsmAI         968  GTCTC                GTCTC(1/5)
BsoBI         142  CCCGAG               C^[CT]CG[GA]G
BspHI        1145  TCATGA               T^CATGA
BstAPI        719  GCAGCCCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI          64  TTCGAA               TT^CGAA
BstEII        999  GGTTACC              G^GT[ACGT]ACC
BstPI         999  GGTTACC              G^GT[ACGT]ACC
BstUI          39  CGCG                 CG^CG
CelII         437  GCTCAGC              GC^T[ACGT]AGC
EagI          227  CGGCCG               C^GGCCG
EclXI         227  CGGCCG               C^GGCCG
Eco52I        227  CGGCCG               C^GGCCG
EcoNI         544  CCTGAGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       999  GGTTACC              G^GT[ACGT]ACC
EcoRI          18  GAATTC               G^AATTC
HgaI         1232  GCGTC                GACGC(5/10)
MaeII        1139  ACGT                 A^CGT
MamI         1405  GATCTAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1223  TGGCCA               TGG^CCA
MscI         1223  TGGCCA               TGG^CCA
MvnI           39  CGCG                 CG^CG
NaeI          225  GCCGGC               GCC^GGC
NcoI          590  CCATGG               C^CATGG
NdeI          314  CATATG               CA^TATG
NgoMIV        225  GCCGGC               G^CCGGC
NspV           64  TTCGAA               TT^CGAA
PflMI        1089  CCAGGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         267  AGGTCCT              [GA]G^G[AT]CC[CT]
RcaI         1145  TCATGA               T^CATGA
SfcI         1020  CTACAG               C^T[GA][CT]AG
SfuI           64  TTCGAA               TT^CGAA
SmlI          819  CTTGAG               C^T[CT][GA]AG
TfiI          248  GAATC                G^A[AT]TC
Tsp509I        19  AATT                 ^AATT
Van91I       1089  CCAGGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          776  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcyI         1138  GACGTC               G[GA]^CG[CT]C
             1231  GGCGTC              
AocI          544  CCTGAGG              CC^T[ACGT]AGG
             1399  CCTCAGG             
ApaI          234  GGGCCC               GGGCC^C
             1051  GGGCCC              
BsaHI        1138  GACGTC               G[GA]^CG[CT]C
             1231  GGCGTC              
BsgI           90  CTGCAC               GTGCAG(16/14)
              478  CTGCAC              
BsmI          294  GAATGC               GAATGC(1/-1)
             1394  GAATGC              
BsrBI          84  CCGCTC               CCGCTC(-3/-3)
              558  GAGCGG              
Bsu36I        544  CCTGAGG              CC^T[ACGT]AGG
             1399  CCTCAGG             
CfoI          186  GCGC                 GCG^C
              347  GCGC                
DsaI          590  CCATGG               C^C[GA][CT]GG
             1226  CCACGG              
EarI          939  CTCTTC               CTCTTC(1/4)
             1184  CTCTTC              
Eco81I        544  CCTGAGG              CC^T[ACGT]AGG
             1399  CCTCAGG             
EcoT14I       590  CCATGG               C^C[AT][AT]GG
             1337  CCAAGG              
HaeII         185  GGCGCT               [GA]GCGC^[CT]
              346  AGCGCC              
HhaI          186  GCGC                 GCG^C
              347  GCGC                
Hin1I        1138  GACGTC               G[GA]^CG[CT]C
             1231  GGCGTC              
HinP1I        186  GCGC                 G^CGC
              347  GCGC                
Ksp632I       939  CTCTTC               CTCTTC(1/4)
             1184  CTCTTC              
SfaNI         534  GATGC                GCATC(5/9)
              990  GCATC               
StyI          590  CCATGG               C^C[AT][AT]GG
             1337  CCAAGG              
TspRI         795  CAGTG                CA[GC]TG[ACGT][ACGT]^
             1461  CAGTG               
XbaI          909  TCTAGA               T^CTAGA
             1407  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AvaII       BanII       BbvI        BcnI        BfaI        
BglI        BglII       BmyI        BpmI        BsaJI       
BsiYI       BslI        BsmFI       Bsp1286I    BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstXI       
BstYI       Cac8I       Cfr10I      Cfr13I      DdeI        
DpnII       DraII       EaeI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        MaeI        
MaeIII      MboI        MboII       MflI        MnlI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       TaqI        TseI        Tsp45I      
TthHB8I     XhoII       
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