Restriction map for pF1KB7772
 (Linear) (Six-base) MAPSORT of: KIBB7772.seq  Check: 6072  from: 1  to: 1515



KIBB7772.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AatII G_ACGT'C

Cuts at:      0    1142    1515 
   Size:       1142     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     845    1394    1515 
   Size:        845     549     121

  Fragments arranged by size: 

                 845     549     121
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     625    1515 
   Size:        625     890
 
AlwNI CAG_nnn'CTG

Cuts at:      0      34     832    1095    1515 
   Size:         34     798     263     420

  Fragments arranged by size: 

                 798     420     263      34
 
ApaI G_GGCC'C

Cuts at:      0     238    1055    1515 
   Size:        238     817     460

  Fragments arranged by size: 

                 817     460     238
 
ApoI r'AATT_y

Cuts at:      0      18    1515 
   Size:         18    1497
 
AvaI C'yCGr_G

Cuts at:      0     142    1515 
   Size:        142    1373
 
BamHI G'GATC_C

Cuts at:      0       3    1515 
   Size:          3    1512
 
BanI G'GyrC_C

Cuts at:      0     673    1515 
   Size:        673     842
 
BanII G_rGCy'C

Cuts at:      0     238     691     929    1055    1515 
   Size:        238     453     238     126     460

  Fragments arranged by size: 

                 460     453     238     238     126
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     186     220    1515 
   Size:        186      34    1295

  Fragments arranged by size: 

                1295     186      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      31     213     695    1515 
   Size:         31     182     482     820

  Fragments arranged by size: 

                 820     482     182      31
 
BglII A'GATC_T

Cuts at:      0     156     377    1410    1515 
   Size:        156     221    1033     105

  Fragments arranged by size: 

                1033     221     156     105
 
BlpI GC'TnA_GC

Cuts at:      0     438    1515 
   Size:        438    1077
 
Bme1580I G_kGCm'C

Cuts at:      0     238    1055    1329    1515 
   Size:        238     817     274     186

  Fragments arranged by size: 

                 817     274     238     186
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     204    1515 
   Size:        204    1311
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1065    1117    1197    1247    1295    1456    1515 
   Size:       1065      52      80      50      48     161      59

  Fragments arranged by size: 

                1065     161      80      59      52      50      48
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     840    1515 
   Size:        840     675
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     973    1515 
   Size:        973     542
 
BsaBI GATnn'nnATC

Cuts at:      0    1409    1515 
   Size:       1409     106
 
BsaHI Gr'CG_yC

Cuts at:      0    1139    1232    1515 
   Size:       1139      93     283

  Fragments arranged by size: 

                1139     283      93
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1012    1042    1515 
   Size:       1012      30     473

  Fragments arranged by size: 

                1012     473      30
 
BseYI C'CCAG_C

Cuts at:      0     239    1041    1194    1270    1515 
   Size:        239     802     153      76     245

  Fragments arranged by size: 

                 802     245     239     153      76
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      75     463    1515 
   Size:         75     388    1052

  Fragments arranged by size: 

                1052     388      75
 
BsiEI CG_ry'CG

Cuts at:      0     230    1515 
   Size:        230    1285
 
BsiHKAI G_wGCw'C

Cuts at:      0    1361    1515 
   Size:       1361     154
 
BsmI GAATG_Cn'

Cuts at:      0     300    1400    1515 
   Size:        300    1100     115

  Fragments arranged by size: 

                1100     300     115
 
Bsp1286I G_dGCh'C

Cuts at:      0     238     691     929    1055    1329    1361    1515 
   Size:        238     453     238     126     274      32     154

  Fragments arranged by size: 

                 453     274     238     238     154     126      32
 
BspHI T'CATG_A

Cuts at:      0    1145    1515 
   Size:       1145     370
 
BsrBI CCG'CTC

Cuts at:      0      86     560    1515 
   Size:         86     474     955

  Fragments arranged by size: 

                 955     474      86
 
BsrFI r'CCGG_y

Cuts at:      0     225     514    1003    1515 
   Size:        225     289     489     512

  Fragments arranged by size: 

                 512     489     289     225
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     725    1515 
   Size:        725     790
 
BstBI TT'CG_AA

Cuts at:      0      65    1515 
   Size:         65    1450
 
BstEII G'GTnAC_C

Cuts at:      0     999    1515 
   Size:        999     516
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1221    1278    1344    1515 
   Size:       1221      57      66     171

  Fragments arranged by size: 

                1221     171      66      57
 
BstYI r'GATC_y

Cuts at:      0       3      54     156     377    1158    1404    1410    1509 
   Size:          3      51     102     221     781     246       6      99

Cuts at:   1509    1515 
   Size:          6

  Fragments arranged by size: 

                 781     246     221     102      99      51       6       6
                   3
 
Bsu36I CC'TnA_GG

Cuts at:      0     545    1400    1515 
   Size:        545     855     115

  Fragments arranged by size: 

                 855     545     115
 
BtgI C'CryG_G

Cuts at:      0     590    1226    1515 
   Size:        590     636     289

  Fragments arranged by size: 

                 636     590     289
 
BtsI GCAGTG_nn'

Cuts at:      0     801    1515 
   Size:        801     714
 
EaeI y'GGCC_r

Cuts at:      0     227     696    1223    1515 
   Size:        227     469     527     292

  Fragments arranged by size: 

                 527     469     292     227
 
EagI C'GGCC_G

Cuts at:      0     227    1515 
   Size:        227    1288
 
EarI CTCTTCn'nnn_

Cuts at:      0     945    1190    1515 
   Size:        945     245     325

  Fragments arranged by size: 

                 945     325     245
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     451     859    1515 
   Size:        451     408     656

  Fragments arranged by size: 

                 656     451     408
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     845    1065    1117    1197    1247    1295    1394    1456 
   Size:        845     220      52      80      50      48      99      62

Cuts at:   1456    1515 
   Size:         59

  Fragments arranged by size: 

                 845     220      99      80      62      59      52      50
                  48
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     548    1515 
   Size:        548     967
 
EcoO109I rG'GnC_Cy

Cuts at:      0     234     235     268    1051    1052    1101    1267    1515 
   Size:        234       1      33     783       1      49     166     248

  Fragments arranged by size: 

                 783     248     234     166      49      33       1       1
            
 
EcoRI G'AATT_C

Cuts at:      0      18    1515 
   Size:         18    1497
 
HaeII r_GCGC'y

Cuts at:      0     189     350    1515 
   Size:        189     161    1165

  Fragments arranged by size: 

                1165     189     161
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     101     133    1515 
   Size:        101      32    1382

  Fragments arranged by size: 

                1382     101      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     706    1204    1515 
   Size:        706     498     311

  Fragments arranged by size: 

                 706     498     311
 
MscI TGG'CCA

Cuts at:      0    1225    1515 
   Size:       1225     290
 
NaeI GCC'GGC

Cuts at:      0     227    1515 
   Size:        227    1288
 
NcoI C'CATG_G

Cuts at:      0     590    1515 
   Size:        590     925
 
NdeI CA'TA_TG

Cuts at:      0     315    1515 
   Size:        315    1200
 
NgoMIV G'CCGG_C

Cuts at:      0     225    1515 
   Size:        225    1290
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1095    1515 
   Size:       1095     420
 
PpuMI rG'GwC_Cy

Cuts at:      0     268    1515 
   Size:        268    1247
 
PspOMI G'GGCC_C

Cuts at:      0     234    1051    1515 
   Size:        234     817     464

  Fragments arranged by size: 

                 817     464     234
 
SfcI C'TryA_G

Cuts at:      0    1020    1515 
   Size:       1020     495
 
SmlI C'TyrA_G

Cuts at:      0     819    1515 
   Size:        819     696
 
StyI C'CwwG_G

Cuts at:      0     590    1337    1515 
   Size:        590     747     178

  Fragments arranged by size: 

                 747     590     178
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     457     489     753     785    1515 
   Size:        457      32     264      32     730

  Fragments arranged by size: 

                 730     457     264      32      32
 
XbaI T'CTAG_A

Cuts at:      0     909    1407    1515 
   Size:        909     498     108

  Fragments arranged by size: 

                 909     498     108
 
XmnI GAAnn'nnTTC

Cuts at:      0     780    1515 
   Size:        780     735
 
ZraI GAC'GTC

Cuts at:      0    1140    1515 
   Size:       1140     375
 

 Enzymes that do cut:

    AatII     AcuI     AlfI    AlwNI     ApaI     ApoI     AvaI    BamHI
     BanI    BanII     BcgI     BglI    BglII     BlpI Bme1580I     BplI
     BpmI    BpuEI     BsaI    BsaBI    BsaHI    BsaXI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrBI    BsrFI   BstAPI
    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI     EaeI
     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII
    Hin4I     MmeI     MscI     NaeI     NcoI     NdeI   NgoMIV    PflMI
    PpuMI   PspOMI     SfcI     SmlI     StyI     TstI     XbaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI    BmgBI
     BmrI     BmtI   Bpu10I    BsaAI    BsaWI    BseRI    BsiWI    BsmBI
    BspEI    BspMI    BsrDI    BsrGI   BssHII    BssSI  BstZ17I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI  EcoICRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MslI   MspA1I     NarI     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI  Tth111I
     XcmI     XhoI     XmaI
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