Restriction map for pF1KB6358
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AviII       AvrII       BalI        BamHI       
BbrPI       BbsI        BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaI        BsaWI       
BseAI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrGI       
BssHII      Bst1107I    BstAPI      BstBI       BstXI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        Eam1105I    
Eco47III    Eco81I      EcoNI       EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KpnI        
KspI        MaeI        MfeI        MluI        MluNI       
MroI        MscI        MunI        NcoI        NdeI        
NheI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacII       SalI        SapI        SexAI       SfaNI       
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SphI        SspBI       SspI        StyI        SwaI        
TfiI        Tsp509I     Van91I      XbaI        XcmI        
XhoI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI          829  GTCTAC               GT^[AC][GT]AC
ApaI          851  GGGCCC               GGGCC^C
Asp700I       498  GAAGTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          823  GACTCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII          99  GGACC                G^G[AT]CC
BglII         755  AGATCT               A^GATCT
BsaBI         750  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI          202  GTGCAG               GTGCAG(16/14)
BsmBI           8  GAGACG               CGTCTC(1/5)
BsmFI         428  GGGAC                GGGAC(10/14)
BsrBI          88  GAGCGG               CCGCTC(-3/-3)
BssSI         679  CACGAG               CACGAG(-5/-1)
BstF5I        749  GGATG                GGATG(2/0)
BstYI         755  AGATCT               [GA]^GATC[CT]
DpnII         756  GATC                 ^GATC
DraII         850  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EarI          388  GAAGAG               CTCTTC(1/4)
EcoO109I      850  GGGGCCC              [GA]G^G[ACGT]CC[CT]
FokI          749  GGATG                GGATG(9/13)
Ksp632I       388  GAAGAG               CTCTTC(1/4)
MamI          750  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          756  GATC                 ^GATC
MboII         388  GAAGA                GAAGA(8/7)
MflI          755  AGATCT               [GA]^GATC[CT]
MseI          662  TTAA                 T^TAA
MslI          600  CATCGTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII         756  GATC                 ^GATC
NlaIII        421  CATG                 CATG^
NotI          121  GCGGCCGC             GC^GGCCGC
PflFI         823  GACTCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI          550  CTGCAG               CTGCA^G
SacI          652  GAGCTC               GAGCT^C
Sau3AI        756  GATC                 ^GATC
SbfI          549  CCTGCAGG             CCTGCA^GG
ScaI          500  AGTACT               AGT^ACT
SfcI          550  CTGCAG               C^T[GA][CT]AG
SmaI          854  CCCGGG               CCC^GGG
SmlI          268  CTCAAG               C^T[CT][GA]AG
Sse8387I      549  CCTGCAGG             CCTGCA^GG
StuI          206  AGGCCT               AGG^CCT
TaqI          806  TCGA                 T^CGA
Tru9I         662  TTAA                 T^TAA
Tth111I       823  GACTCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       806  TCGA                 T^CGA
XhoII         755  AGATCT               [GA]^GATC[CT]
XmaCI         854  CCCGGG               C^CCGGG
XmaI          854  CCCGGG               C^CCGGG
XmnI          498  GAAGTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

BanII         652  GAGCTC               G[GA]GC[CT]^C
              851  GGGCCC              
BbeI          250  GGCGCC               GGCGC^C
              356  GGCGCC              
BbvCI          79  GCTGAGG              CCTCAGC(-5/-2)
              556  GCTGAGG             
BpmI          435  CTGGAG               CTGGAG(16/14)
              455  CTGGAG              
BsmAI           8  GAGAC                GTCTC(1/5)
              458  GAGAC               
BspMI         548  ACCTGC               ACCTGC(4/8)
              711  GCAGGT              
BsrFI         186  GCCGGC               [GA]^CCGG[CT]
              594  GCCGGC              
BstEII         13  GGTGACC              G^GT[ACGT]ACC
              858  GGTGACC             
BstPI          13  GGTGACC              G^GT[ACGT]ACC
              858  GGTGACC             
Cfr10I        186  GCCGGC               [GA]^CCGG[CT]
              594  GCCGGC              
EcoO65I        13  GGTGACC              G^GT[ACGT]ACC
              858  GGTGACC             
HaeII         250  GGCGCC               [GA]GCGC^[CT]
              356  GGCGCC              
HinfI         588  GACTC                G^A[ACGT]TC
              823  GACTC               
KasI          250  GGCGCC               G^GCGCC
              356  GGCGCC              
MaeII         413  ACGT                 A^CGT
              686  ACGT                
NaeI          186  GCCGGC               GCC^GGC
              594  GCCGGC              
NarI          250  GGCGCC               GG^CGCC
              356  GGCGCC              
NgoMIV        186  GCCGGC               G^CCGGC
              594  GCCGGC              
PleI          588  GACTC                GAGTC(4/5)
              823  GACTC               
SfoI          250  GGCGCC               GGC^GCC
              356  GGCGCC              


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AspHI       AvaI        BanI        BbvI        BcnI        
BmyI        BsaHI       BsaJI       BseRI       BsiEI       
BsiHKAI     BsiYI       BslI        BsoBI       Bsp1286I    
BsrI        BssKI       BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        EaeI        EagI        
EclXI       Eco52I      EcoRII      FauI        Fnu4HI      
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HpaII       HphI        ItaI        MaeIII      
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NlaIV       RsaI        Sau96I      
ScrFI       TseI        Tsp45I      TspRI       
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