Restriction map for pF1KE0507
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
BamHI       BanI        BbeI        BbrPI       BbsI        
BbvCI       BciVI       BclI        BfrI        BglII       
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HaeII       Hin1I       HincII      HindII      HpaI        
KasI        KpnI        KspI        MaeII       MaeIII      
MamI        MfeI        MflI        MluI        MroI        
MseI        MslI        MunI        NarI        NcoI        
NdeI        NheI        NotI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        TaqI        TfiI        Tru9I       Tsp45I      
Tsp509I     Tth111I     TthHB8I     XbaI        XcmI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AfaI          382  GTAC                 GT^AC
AviII          71  TGCGCA               TGC^GCA
AvrII         627  CCTAGG               C^CTAGG
BcgI          246  CGAGGAGTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          628  CTAG                 C^TAG
BlnI          627  CCTAGG               C^CTAGG
BsaI          658  GAGACC               GGTCTC(1/5)
BsgI          213  CTGCAC               GTGCAG(16/14)
BsmAI         658  GAGAC                GTCTC(1/5)
BsrBI         438  CCGCTC               CCGCTC(-3/-3)
BsrFI         315  GCCGGC               [GA]^CCGG[CT]
BstF5I        443  CATCC                GGATG(2/0)
Cfr10I        315  GCCGGC               [GA]^CCGG[CT]
DpnII         702  GATC                 ^GATC
EarI          729  GAAGAG               CTCTTC(1/4)
FokI          443  CATCC                GGATG(9/13)
FspI           71  TGCGCA               TGC^GCA
HgaI          409  GACGC                GACGC(5/10)
HindIII       357  AAGCTT               A^AGCTT
HphI          336  GGTGA                GGTGA(8/7)
Ksp632I       729  GAAGAG               CTCTTC(1/4)
MaeI          628  CTAG                 C^TAG
MboI          702  GATC                 ^GATC
NaeI          315  GCCGGC               GCC^GGC
NdeII         702  GATC                 ^GATC
NgoMIV        315  GCCGGC               G^CCGGC
NruI          171  TCGCGA               TCG^CGA
NspI          368  ACATGC               [GA]CATG^[CT]
PflMI         417  CCAGAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI          91  GGGACCC              [GA]G^G[AT]CC[CT]
RsaI          382  GTAC                 GT^AC
Sau3AI        702  GATC                 ^GATC
SfiI          321  GGCCCAGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          350  CCCGGG               CCC^GGG
SmlI          139  CTCAAG               C^T[CT][GA]AG
StuI          329  AGGCCT               AGG^CCT
Van91I        417  CCAGAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         350  CCCGGG               C^CCGGG
XmaI          350  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccII         172  CGCG                 CG^CG
              385  CGCG                
AlwNI         418  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              462  CAGCCGCTG           
AvaI          244  CCCGAG               C^[CT]CG[GA]G
              350  CCCGGG              
AvaII          54  GGACC                G^G[AT]CC
               92  GGACC               
BalI          513  TGGCCA               TGG^CCA
              693  TGGCCA              
BglI          322  GCCCAGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              539  GCCCTGTGGGC         
BpmI          281  CTGGAG               CTGGAG(16/14)
              505  CTGGAG              
BsoBI         244  CCCGAG               C^[CT]CG[GA]G
              350  CCCGGG              
BstUI         172  CGCG                 CG^CG
              385  CGCG                
EcoNI         522  CCTCTCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              627  CCTAGGCAAGG         
EcoT14I       527  CCAAGG               C^C[AT][AT]GG
              627  CCTAGG              
FauI          499  GCGGG                CCCGC(4/6)
              562  GCGGG               
HinfI          43  GACTC                G^A[ACGT]TC
              168  GAGTC               
MluNI         513  TGGCCA               TGG^CCA
              693  TGGCCA              
MscI          513  TGGCCA               TGG^CCA
              693  TGGCCA              
MspA1I         50  CAGCGG               C[AC]G^C[GT]G
              465  CCGCTG              
MvnI          172  CGCG                 CG^CG
              385  CGCG                
PleI           43  GACTC                GAGTC(4/5)
              168  GAGTC               
SfaNI         125  GATGC                GCATC(5/9)
              274  GATGC               
StyI          527  CCAAGG               C^C[AT][AT]GG
              627  CCTAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AciI        AluI        ApaI        BanII       BbvI        
BcnI        BmyI        BsaJI       BseRI       BsiYI       
BslI        BsmFI       Bsp1286I    BspMI       BsrI        
BssKI       BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DraII       EaeI        EcoO109I    EcoRII      
Fnu4HI      HaeIII      HapII       HhaI        HinP1I      
HpaII       ItaI        MboII       MnlI        MspI        
MvaI        MwoI        NciI        NlaIII      NlaIV       
PstI        Sau96I      ScrFI       SfcI        TseI        
TspRI       
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