Restriction map for pF1KE0507
 (Circular) (Six-base) MAPSORT of: pF1KE0507.seq  Check: 6334  from: 1  to: 3890



pF1KE0507  3890 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    413     413 
   Size:       3890
 
AccI GT'mk_AC

Cuts at:    928     928 
   Size:       3890
 
Acc65I G'GTAC_C

Cuts at:    906     906 
   Size:       3890
 
AclI AA'CG_TT

Cuts at:   3730    3730 
   Size:       3890
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1715    2147    2894    3162    1715 
   Size:        432     747     268    2443

  Fragments arranged by size: 

                2443     747     432     268
 
AflIII A'CryG_T

Cuts at:   1242    2346    3323    1242 
   Size:       1104     977    1809

  Fragments arranged by size: 

                1809    1104     977
 
AgeI A'CCGG_T

Cuts at:   2278    2278 
   Size:       3890
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    737     737 
   Size:       3890
 
AlwNI CAG_nnn'CTG

Cuts at:    531     575    1195    2762     531 
   Size:         44     620    1567    1659

  Fragments arranged by size: 

                1659    1567     620      44
 
ApaI G_GGCC'C

Cuts at:    196     294     721     846     196 
   Size:         98     427     125    3240

  Fragments arranged by size: 

                3240     427     125      98
 
ApaLI G'TGCA_C

Cuts at:   2660    2660 
   Size:       3890
 
ApoI r'AATT_y

Cuts at:    894    3243    3499     894 
   Size:       2349     256    1285

  Fragments arranged by size: 

                2349    1285     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3890
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3890
 
AvaI C'yCGr_G

Cuts at:    352     458     910     352 
   Size:        106     452    3332

  Fragments arranged by size: 

                3332     452     106
 
AvrII C'CTAG_G

Cuts at:    735     735 
   Size:       3890
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    481     514     481 
   Size:         33    3857
 
BamHI G'GATC_C

Cuts at:    915     915 
   Size:       3890
 
BanI G'GyrC_C

Cuts at:    906    1320    1568    1603     906 
   Size:        414     248      35    3193

  Fragments arranged by size: 

                3193     414     248      35
 
BanII G_rGCy'C

Cuts at:    196     294     721     754     846     904    1934     196 
   Size:         98     427      33      92      58    1030    2152

  Fragments arranged by size: 

                2152    1030     427      98      92      58      33
 
BbeI G_GCGC'C

Cuts at:   1324    1572    1324 
   Size:        248    3642
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    343     377     343 
   Size:         34    3856
 
BciVI GTATCCnnnnn_n'

Cuts at:   1788    2549    3890    1788 
   Size:        761    1341    1788

  Fragments arranged by size: 

                1788    1341     761
 
BclI T'GATC_A

Cuts at:   1410    1410 
   Size:       3890
 
BglI GCCn_nnn'nGGC

Cuts at:    436     653     436 
   Size:        217    3673
 
BglII A'GATC_T

Cuts at:   1405    3442    1405 
   Size:       2037    1853
 
BlpI GC'TnA_GC

Cuts at:   1002    1002 
   Size:       3890
 
Bme1580I G_kGCm'C

Cuts at:    196     294     353     721     846    1515    1608    2664     196 
   Size:         98      59     368     125     669      93    1056    1422

  Fragments arranged by size: 

                1422    1056     669     368     125      98      93      59
            
 
BmrI ACTGGGnnnn_n'

Cuts at:    209    1102    1183    1518    3662     209 
   Size:        893      81     335    2144     437

  Fragments arranged by size: 

                2144     893     437     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2257    2257 
   Size:       3890
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    410     634     410 
   Size:        224    3666
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    232    1066    1945    2437    2735     232 
   Size:        834     879     492     298    1387

  Fragments arranged by size: 

                1387     879     834     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    760    3594     760 
   Size:       2834    1056
 
BsaAI yAC'GTr

Cuts at:   1243    1243 
   Size:       3890
 
BsaBI GATnn'nnATC

Cuts at:      6    1404    3441       6 
   Size:       1398    2037     455

  Fragments arranged by size: 

                2037    1398     455
 
BsaHI Gr'CG_yC

Cuts at:   1321    1569    2271    3767    1321 
   Size:        248     702    1496    1444

  Fragments arranged by size: 

                1496    1444     702     248
 
BsaWI w'CCGG_w

Cuts at:   1079    1600    2278    2552    2699    3110    1079 
   Size:        521     678     274     147     411    1859

  Fragments arranged by size: 

                1859     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    493     523     616     646     493 
   Size:         30      93      30    3737

  Fragments arranged by size: 

                3737      93      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    138     370     721     138 
   Size:        232     351    3307

  Fragments arranged by size: 

                3307     351     232
 
BseYI C'CCAG_C

Cuts at:    156    1315    2073    2650    3146     156 
   Size:       1159     758     577     496     900

  Fragments arranged by size: 

                1159     900     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    306     306 
   Size:       3890
 
BsiEI CG_ry'CG

Cuts at:    104    1432    1478    2686     104 
   Size:       1328      46    1208    1308

  Fragments arranged by size: 

                1328    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    904    1682    1872    2664     904 
   Size:        778     190     792    2130

  Fragments arranged by size: 

                2130     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    196     294     353     721     754     846     904    1515    1608 
   Size:         98      59     368      33      92      58     611      93

Cuts at:   1608    1682    1872    1934    2664     196 
   Size:         74     190      62     730    1422

  Fragments arranged by size: 

                1422     730     611     368     190      98      93      92
                  74      62      59      58      33
 
BspEI T'CCGG_A

Cuts at:   3110    3110 
   Size:       3890
 
BspHI T'CATG_A

Cuts at:   3066    3066 
   Size:       3890
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    266     413     668     940    1154    1456    1837     266 
   Size:        147     255     272     214     302     381    2319

  Fragments arranged by size: 

                2319     381     302     272     255     214     147
 
BsrBI CCG'CTC

Cuts at:     50     548    2182    2236    3720    3885      50 
   Size:        498    1634      54    1484     165      55

  Fragments arranged by size: 

                1634    1484     498     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1802    1802 
   Size:       3890
 
BsrFI r'CCGG_y

Cuts at:    423    1888    2069    2278     423 
   Size:       1465     181     209    2035

  Fragments arranged by size: 

                2035    1465     209     181
 
BssSI C'ACGA_G

Cuts at:   2161    2519    2161 
   Size:        358    3532
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1267    1267 
   Size:       3890
 
BstBI TT'CG_AA

Cuts at:   2251    2251 
   Size:       3890
 
BstYI r'GATC_y

Cuts at:      1     915    1382    1405    1740    1986    2998    3084    3096 
   Size:        914     467      23     335     246    1012      86      12

Cuts at:   3096    3442       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     914     467     449     346     335     246      86
                  23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1840    2021    2184    1840 
   Size:        181     163    3546

  Fragments arranged by size: 

                3546     181     163
 
BtsI GCAGTG_nn'

Cuts at:    624     832    1133     624 
   Size:        208     301    3381

  Fragments arranged by size: 

                3381     301     208
 
DraI TTT'AAA

Cuts at:    889     889 
   Size:       3890
 
DrdI GACnn_nn'nnGTC

Cuts at:   1596    2454    1596 
   Size:        858    3032
 
EaeI y'GGCC_r

Cuts at:    225     335     621     801    1475    1649    2040    2067    3412 
   Size:        110     286     180     674     174     391      27    1345

Cuts at:   3412     225 
   Size:        703

  Fragments arranged by size: 

                1345     703     674     391     286     180     174     110
                  27
 
EagI C'GGCC_G

Cuts at:   1475    1475 
   Size:       3890
 
EarI CTCTTCn'nnn_

Cuts at:    832    1913    2123     832 
   Size:       1081     210    2599

  Fragments arranged by size: 

                2599    1081     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2098    2408    2554    3700    2098 
   Size:        310     146    1146    2288

  Fragments arranged by size: 

                2288    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    902     902 
   Size:       3890
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    410     634    1715    2147    2894    3162     410 
   Size:        224    1081     432     747     268    1138

  Fragments arranged by size: 

                1138    1081     747     432     268     224
 
EcoNI CCTnn'n_nnAGG

Cuts at:    634     739     634 
   Size:        105    3785
 
EcoO109I rG'GnC_Cy

Cuts at:    192     200     291     717     718     842    1029     192 
   Size:          8      91     426       1     124     187    3053

  Fragments arranged by size: 

                3053     426     187     124      91       8       1
 
EcoRI G'AATT_C

Cuts at:    894     894 
   Size:       3890
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1909    1909 
   Size:       3890
 
FspI TGC'GCA

Cuts at:    181    1671     181 
   Size:       1490    2400
 
HaeII r_GCGC'y

Cuts at:   1324    1572    2594    3573    1324 
   Size:        248    1022     979    1641

  Fragments arranged by size: 

                1641    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1732    1762    1764    1794    1732 
   Size:         30       2      30    3828

  Fragments arranged by size: 

                3828      30      30       2
 
HincII GTy'rAC

Cuts at:    929     929 
   Size:       3890
 
HindIII A'AGCT_T

Cuts at:    465     465 
   Size:       3890
 
KasI G'GCGC_C

Cuts at:   1320    1568    1320 
   Size:        248    3642
 
KpnI G_GTAC'C

Cuts at:    910     910 
   Size:       3890
 
MluI A'CGCG_T

Cuts at:   3323    3323 
   Size:       3890
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    479    2561    2745     479 
   Size:       2082     184    1624

  Fragments arranged by size: 

                2082    1624     184
 
MscI TGG'CCA

Cuts at:    623     803    1651     623 
   Size:        180     848    2862

  Fragments arranged by size: 

                2862     848     180
 
MslI CAynn'nnrTG

Cuts at:   1879    2006    1879 
   Size:        127    3763
 
MspA1I CmG'CkG

Cuts at:    160     575    1001    1315    1675    2688    2933     160 
   Size:        415     426     314     360    1013     245    1117

  Fragments arranged by size: 

                1117    1013     426     415     360     314     245
 
NaeI GCC'GGC

Cuts at:    425    2071     425 
   Size:       1646    2244
 
NarI GG'CG_CC

Cuts at:   1321    1569    1321 
   Size:        248    3642
 
NgoMIV G'CCGG_C

Cuts at:    423    2069     423 
   Size:       1646    2244
 
NruI TCG'CGA

Cuts at:    281     281 
   Size:       3890
 
NspI r_CATG'y

Cuts at:    480     943    1443    2350     480 
   Size:        463     500     907    2020

  Fragments arranged by size: 

                2020     907     500     463
 
PasI CC'CwG_GG

Cuts at:    196     846     196 
   Size:        650    3240
 
PciI A'CATG_T

Cuts at:   2346    2346 
   Size:       3890
 
PflMI CCAn_nnn'nTGG

Cuts at:    531     531 
   Size:       3890
 
PfoI T'CCnGG_A

Cuts at:    553     553 
   Size:       3890
 
PmeI GTTT'AAAC

Cuts at:    889     889 
   Size:       3890
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3029    3061    3029 
   Size:         32    3858
 
PpuMI rG'GwC_Cy

Cuts at:    200     200 
   Size:       3890
 
PspOMI G'GGCC_C

Cuts at:    192     290     717     842     192 
   Size:         98     427     125    3240

  Fragments arranged by size: 

                3240     427     125      98
 
PstI C_TGCA'G

Cuts at:    417     687     716     937    1622     417 
   Size:        270      29     221     685    2685

  Fragments arranged by size: 

                2685     685     270     221      29
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3890
 
PvuII CAG'CTG

Cuts at:   1315    1675    1315 
   Size:        360    3530
 
RsrII CG'GwC_CG

Cuts at:   2085    2085 
   Size:       3890
 
SacI G_AGCT'C

Cuts at:    904     904 
   Size:       3890
 
SalI G'TCGA_C

Cuts at:    927     927 
   Size:       3890
 
SanDI GG'GwC_CC

Cuts at:    200     200 
   Size:       3890
 
SapI GCTCTTCn'nnn_

Cuts at:   1913    2123    1913 
   Size:        210    3680
 
SbfI CC_TGCA'GG

Cuts at:    937     937 
   Size:       3890
 
ScaI AGT'ACT

Cuts at:   3407    3407 
   Size:       3890
 
SfcI C'TryA_G

Cuts at:     33     413     683     712     933    1618    2611    2802      33 
   Size:        380     270      29     221     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     380     270     221     191      29
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    436     436 
   Size:       3890
 
SfoI GGC'GCC

Cuts at:   1322    1570    1322 
   Size:        248    3642
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3890
 
SmaI CCC'GGG

Cuts at:    460     912     460 
   Size:        452    3438
 
SmlI C'TyrA_G

Cuts at:    247    1045    1960    2452    2714     247 
   Size:        798     915     492     262    1423

  Fragments arranged by size: 

                1423     915     798     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3890
 
SphI G_CATG'C

Cuts at:    943    1443     943 
   Size:        500    3390
 
StuI AGG'CCT

Cuts at:    439     439 
   Size:       3890
 
StyI C'CwwG_G

Cuts at:    635     735    1024    1378     635 
   Size:        100     289     354    3147

  Fragments arranged by size: 

                3147     354     289     100
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    432    1473    1606    2065    2274     432 
   Size:       1041     133     459     209    2048

  Fragments arranged by size: 

                2048    1041     459     209     133
 
TatI w'GTAC_w

Cuts at:   3405    3405 
   Size:       3890
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    616     648    3407    3439     616 
   Size:         32    2759      32    1067

  Fragments arranged by size: 

                2759    1067      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1687    1687 
   Size:       3890
 
XbaI T'CTAG_A

Cuts at:    921     921 
   Size:       3890
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1385    1385 
   Size:       3890
 
XmaI C'CCGG_G

Cuts at:    458     910     458 
   Size:        452    3438
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI    BanII     BbeI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI   NgoMIV     NruI     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PpuMI   PspOMI     PstI     PvuI    PvuII
    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI     SfcI
     SfiI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AloI     AscI     BbsI
    BbvCI    BfrBI    BmgBI     BmtI   Bpu10I    BsiWI     BsmI    BsmBI
    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     ClaI
    CspCI   DraIII    EcoRV     FseI    FspAI     HpaI     MfeI     NcoI
     NdeI     NheI     NotI     NsiI     PacI     PmlI    PshAI     PsiI
    PspXI     PsrI    SacII    SexAI    SgrAI    SnaBI     SrfI     SspI
     SwaI     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com