Restriction map for pF1KB6305
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AflII       AflIII      AhdI        Alw44I      AlwI        
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaHI       
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspHI       BspLU11I    
BsrBI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstYI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DrdI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MamI        MfeI        
MflI        MluI        MluNI       MscI        MseI        
MunI        NarI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        TfiI        Tru9I       
Tth111I     TthHB8I     XbaI        XhoI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccIII         36  TCCGGA               T^CCGGA
AcsI           81  GAATTC               [GA]^AATT[CT]
AgeI          113  ACCGGT               A^CCGGT
ApaI          621  GGGCCC               GGGCC^C
ApoI           81  GAATTC               [GA]^AATT[CT]
Asp700I       204  GAAGACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BglI          409  GCCATCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         265  TACGTG               [CT]AC^GT[GA]
BseAI          36  TCCGGA               T^CCGGA
BseRI         534  CTCCTC               GAGGAG(10/8)
BsgI          274  GTGCAG               GTGCAG(16/14)
BspEI          36  TCCGGA               T^CCGGA
BspMI         500  ACCTGC               ACCTGC(4/8)
BsrDI         631  CATTGC               GCAATG(2/0)
BstXI         243  CCAGAATGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          345  GCGC                 GCG^C
DpnII         282  GATC                 ^GATC
DraIII        187  CACTGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI         486  CCTGCAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI          81  GAATTC               G^AATTC
FauI           50  CCCGC                CCCGC(4/6)
HhaI          345  GCGC                 GCG^C
HinP1I        345  GCGC                 G^CGC
HinfI         613  GACTC                G^A[ACGT]TC
MboI          282  GATC                 ^GATC
MroI           36  TCCGGA               T^CCGGA
NaeI          322  GCCGGC               GCC^GGC
NdeII         282  GATC                 ^GATC
NgoMIV        322  GCCGGC               G^CCGGC
PflMI         630  CCATTGCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         113  ACCGGT               A^CCGGT
PleI          613  GACTC                GAGTC(4/5)
PstI          487  CTGCAG               CTGCA^G
Sau3AI        282  GATC                 ^GATC
Tsp509I        82  AATT                 ^AATT
Van91I        630  CCATTGCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          204  GAAGACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccII          52  CGCG                 CG^CG
              214  CGCG                
AciI           51  CCGC                 CCGC(-3/-1)
              213  CCGC                
AfaI          111  GTAC                 GT^AC
              264  GTAC                
AlwNI         426  CAGTCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              481  CAGACCCTG           
BanII         546  GGGCTC               G[GA]GC[CT]^C
              621  GGGCCC              
BbsI          204  GAAGAC               GAAGAC(2/6)
              718  GTCTTC              
BmyI          546  GGGCTC               G[AGT]GC[ACT]^C
              621  GGGCCC              
BpuAI         204  GAAGAC               GAAGAC(2/6)
              718  GTCTTC              
BsaI          227  GAGACC               GGTCTC(1/5)
              729  GGTCTC              
BsaWI          36  TCCGGA               [AT]^CCGG[AT]
              113  ACCGGT              
Bsp1286I      546  GGGCTC               G[AGT]GC[ACT]^C
              621  GGGCCC              
BsrFI         113  ACCGGT               [GA]^CCGG[CT]
              322  GCCGGC              
BstUI          52  CGCG                 CG^CG
              214  CGCG                
Cfr10I        113  ACCGGT               [GA]^CCGG[CT]
              322  GCCGGC              
DsaI          566  CCATGG               C^C[GA][CT]GG
              660  CCATGG              
EcoT14I       566  CCATGG               C^C[AT][AT]GG
              660  CCATGG              
MaeII         266  ACGT                 A^CGT
              314  ACGT                
MslI          136  CACACCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              564  CACCATGGTG          
MvnI           52  CGCG                 CG^CG
              214  CGCG                
MwoI          409  GCCATCCTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              596  GCTCTGGATGC         
NcoI          566  CCATGG               C^CATGG
              660  CCATGG              
NlaIII        567  CATG                 CATG^
              661  CATG                
PpuMI          29  AGGACCT              [GA]G^G[AT]CC[CT]
              148  GGGACCC             
RsaI          111  GTAC                 GT^AC
              264  GTAC                
SfaNI         602  GATGC                GCATC(5/9)
              291  GCATC               
SfcI          487  CTGCAG               C^T[GA][CT]AG
              713  CTACAG              
StyI          566  CCATGG               C^C[AT][AT]GG
              660  CCATGG              
Tsp45I        611  GTGAC                ^GT[GC]AC
              667  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AluI        AvaII       BbvI        BcnI        BpmI        
BsaJI       BsiYI       BslI        BsmAI       BsmFI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DraII       EcoO109I    EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HpaII       
HphI        ItaI        MaeIII      MboII       MnlI        
MspA1I      MspI        MvaI        NciI        NlaIV       
PvuII       Sau96I      ScrFI       TseI        TspRI       
XcmI        
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