Restriction map for pF1KB6305
 (Circular) (Six-base) MAPSORT of: pF1KB6305.seq  Check: 127  from: 1  to: 3887



pF1KB6305  3887 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AccI GT'mk_AC

Cuts at:    925     925 
   Size:       3887
 
Acc65I G'GTAC_C

Cuts at:    903     903 
   Size:       3887
 
AclI AA'CG_TT

Cuts at:    881    3727     881 
   Size:       2846    1041
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1712    2144    2891    3159    1712 
   Size:        432     747     268    2440

  Fragments arranged by size: 

                2440     747     432     268
 
AflIII A'CryG_T

Cuts at:   1239    2343    3320    1239 
   Size:       1104     977    1806

  Fragments arranged by size: 

                1806    1104     977
 
AgeI A'CCGG_T

Cuts at:    221    2275     221 
   Size:       2054    1833
 
AleI CACnn'nnGTG

Cuts at:    248     676     248 
   Size:        428    3459
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    683     715     683 
   Size:         32    3855
 
AlwNI CAG_nnn'CTG

Cuts at:    539     594    1192    2759     539 
   Size:         55     598    1567    1667

  Fragments arranged by size: 

                1667    1567     598      55
 
ApaI G_GGCC'C

Cuts at:    733     733 
   Size:       3887
 
ApaLI G'TGCA_C

Cuts at:   2657    2657 
   Size:       3887
 
ApoI r'AATT_y

Cuts at:    189     891    3240    3496     189 
   Size:        702    2349     256     580

  Fragments arranged by size: 

                2349     702     580     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3887
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3887
 
AvaI C'yCGr_G

Cuts at:    907     907 
   Size:       3887
 
BamHI G'GATC_C

Cuts at:    912     912 
   Size:       3887
 
BanI G'GyrC_C

Cuts at:    903    1317    1565    1600     903 
   Size:        414     248      35    3190

  Fragments arranged by size: 

                3190     414     248      35
 
BanII G_rGCy'C

Cuts at:    658     733     901    1931     658 
   Size:         75     168    1030    2614

  Fragments arranged by size: 

                2614    1030     168      75
 
BbeI G_GCGC'C

Cuts at:   1321    1569    1321 
   Size:        248    3639
 
BbsI GAAGACnn'nnnn_

Cuts at:    319     819     319 
   Size:        500    3387
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    3853
 
BciVI GTATCCnnnnn_n'

Cuts at:   1785    2546    3887    1785 
   Size:        761    1341    1785

  Fragments arranged by size: 

                1785    1341     761
 
BclI T'GATC_A

Cuts at:   1407    1407 
   Size:       3887
 
BglI GCCn_nnn'nGGC

Cuts at:    523     523 
   Size:       3887
 
BglII A'GATC_T

Cuts at:   1402    3439    1402 
   Size:       2037    1850
 
BlpI GC'TnA_GC

Cuts at:    999     999 
   Size:       3887
 
Bme1580I G_kGCm'C

Cuts at:    733    1512    1605    2661     733 
   Size:        779      93    1056    1959

  Fragments arranged by size: 

                1959    1056     779      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    285     660    1099    1180    1515    3659     285 
   Size:        375     439      81     335    2144     513

  Fragments arranged by size: 

                2144     513     439     375     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2254    2254 
   Size:       3887
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    199     248     353     621     795     199 
   Size:         49     105     268     174    3291

  Fragments arranged by size: 

                3291     268     174     105      49
 
Bpu10I CC'TnA_GC

Cuts at:    346     346 
   Size:       3887
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1063    1942    2434    2732    1063 
   Size:        879     492     298    2218

  Fragments arranged by size: 

                2218     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    329     843    3591     329 
   Size:        514    2748     625

  Fragments arranged by size: 

                2748     625     514
 
BsaAI yAC'GTr

Cuts at:    375    1240     375 
   Size:        865    3022
 
BsaBI GATnn'nnATC

Cuts at:      6    1401    3438       6 
   Size:       1395    2037     455

  Fragments arranged by size: 

                2037    1395     455
 
BsaHI Gr'CG_yC

Cuts at:   1318    1566    2268    3764    1318 
   Size:        248     702    1496    1441

  Fragments arranged by size: 

                1496    1441     702     248
 
BsaWI w'CCGG_w

Cuts at:    144     221    1076    1597    2275    2549    2696    3107     144 
   Size:         77     855     521     678     274     147     411     924

  Fragments arranged by size: 

                 924     855     678     521     411     274     147      77
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    640     670     683     713     640 
   Size:         30      13      30    3814

  Fragments arranged by size: 

                3814      30      30      13
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    633     633 
   Size:       3887
 
BseYI C'CCAG_C

Cuts at:    492     554    1312    2070    2647    3143     492 
   Size:         62     758     758     577     496    1236

  Fragments arranged by size: 

                1236     758     758     577     496      62
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    403     403 
   Size:       3887
 
BsiEI CG_ry'CG

Cuts at:    104    1429    1475    2683     104 
   Size:       1325      46    1208    1308

  Fragments arranged by size: 

                1325    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    901    1679    1869    2661     901 
   Size:        778     190     792    2127

  Fragments arranged by size: 

                2127     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    658     733     901    1512    1605    1679    1869    1931    2661 
   Size:         75     168     611      93      74     190      62     730

Cuts at:   2661     658 
   Size:       1884

  Fragments arranged by size: 

                1884     730     611     190     168      93      75      74
                  62
 
BspEI T'CCGG_A

Cuts at:    144    3107     144 
   Size:       2963     924
 
BspHI T'CATG_A

Cuts at:   3063    3063 
   Size:       3887
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    617     937    1151    1453    1834     617 
   Size:        320     214     302     381    2670

  Fragments arranged by size: 

                2670     381     320     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2179    2233    3717    3882      50 
   Size:       2129      54    1484     165      55

  Fragments arranged by size: 

                2129    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    738    1799     738 
   Size:       1061    2826
 
BsrFI r'CCGG_y

Cuts at:    221     430    1885    2066    2275     221 
   Size:        209    1455     181     209    1833

  Fragments arranged by size: 

                1833    1455     209     209     181
 
BssSI C'ACGA_G

Cuts at:   2158    2516    2158 
   Size:        358    3529
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1264    1264 
   Size:       3887
 
BstBI TT'CG_AA

Cuts at:   2248    2248 
   Size:       3887
 
BstXI CCAn_nnnn'nTGG

Cuts at:    358     358 
   Size:       3887
 
BstYI r'GATC_y

Cuts at:      1     912    1379    1402    1737    1983    2995    3081    3093 
   Size:        911     467      23     335     246    1012      86      12

Cuts at:   3093    3439       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     911     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    674     768     674 
   Size:         94    3793
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    426     855    1837    2018    2181     426 
   Size:        429     982     181     163    2132

  Fragments arranged by size: 

                2132     982     429     181     163
 
BtsI GCAGTG_nn'

Cuts at:    294    1130     294 
   Size:        836    3051
 
DraI TTT'AAA

Cuts at:    886     886 
   Size:       3887
 
DraIII CAC_nnn'GTG

Cuts at:    300     300 
   Size:       3887
 
DrdI GACnn_nn'nnGTC

Cuts at:   1593    2451    1593 
   Size:        858    3029
 
EaeI y'GGCC_r

Cuts at:   1472    1646    2037    2064    3409    1472 
   Size:        174     391      27    1345    1950

  Fragments arranged by size: 

                1950    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1472    1472 
   Size:       3887
 
EarI CTCTTCn'nnn_

Cuts at:   1910    2120    1910 
   Size:        210    3677
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2095    2405    2551    3697    2095 
   Size:        310     146    1146    2285

  Fragments arranged by size: 

                2285    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    899     899 
   Size:       3887
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    199     248     353     621     795    1712    2144    2891    3159 
   Size:         49     105     268     174     917     432     747     268

Cuts at:   3159     199 
   Size:        927

  Fragments arranged by size: 

                 927     917     747     432     268     268     174     105
                  49
 
EcoNI CCTnn'n_nnAGG

Cuts at:    598     598 
   Size:       3887
 
EcoO109I rG'GnC_Cy

Cuts at:    138     257     729     730    1026     138 
   Size:        119     472       1     296    2999

  Fragments arranged by size: 

                2999     472     296     119       1
 
EcoRI G'AATT_C

Cuts at:    189     891     189 
   Size:        702    3185
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1906    1906 
   Size:       3887
 
FspI TGC'GCA

Cuts at:   1668    1668 
   Size:       3887
 
HaeII r_GCGC'y

Cuts at:   1321    1569    2591    3570    1321 
   Size:        248    1022     979    1638

  Fragments arranged by size: 

                1638    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    276     308    1729    1759    1761    1791     276 
   Size:         32    1421      30       2      30    2372

  Fragments arranged by size: 

                2372    1421      32      30      30       2
 
HincII GTy'rAC

Cuts at:    926     926 
   Size:       3887
 
KasI G'GCGC_C

Cuts at:   1317    1565    1317 
   Size:        248    3639
 
KpnI G_GTAC'C

Cuts at:    907     907 
   Size:       3887
 
MluI A'CGCG_T

Cuts at:   3320    3320 
   Size:       3887
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    147     902    2558    2742     147 
   Size:        755    1656     184    1292

  Fragments arranged by size: 

                1656    1292     755     184
 
MscI TGG'CCA

Cuts at:   1648    1648 
   Size:       3887
 
MslI CAynn'nnrTG

Cuts at:    248     676    1876    2003     248 
   Size:        428    1200     127    2132

  Fragments arranged by size: 

                2132    1200     428     127
 
MspA1I CmG'CkG

Cuts at:    289     492     582     998    1312    1672    2685    2930     289 
   Size:        203      90     416     314     360    1013     245    1246

  Fragments arranged by size: 

                1246    1013     416     360     314     245     203      90
            
 
NaeI GCC'GGC

Cuts at:    432    2068     432 
   Size:       1636    2251
 
NarI GG'CG_CC

Cuts at:   1318    1566    1318 
   Size:        248    3639
 
NcoI C'CATG_G

Cuts at:    674     768     674 
   Size:         94    3793
 
NgoMIV G'CCGG_C

Cuts at:    430    2066     430 
   Size:       1636    2251
 
NspI r_CATG'y

Cuts at:    940    1440    2347     940 
   Size:        500     907    2480

  Fragments arranged by size: 

                2480     907     500
 
PciI A'CATG_T

Cuts at:   2343    2343 
   Size:       3887
 
PflMI CCAn_nnn'nTGG

Cuts at:    744     744 
   Size:       3887
 
PfoI T'CCnGG_A

Cuts at:    811     853     811 
   Size:         42    3845
 
PmeI GTTT'AAAC

Cuts at:    886     886 
   Size:       3887
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    683     715    3026    3058     683 
   Size:         32    2311      32    1512

  Fragments arranged by size: 

                2311    1512      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    138     257     138 
   Size:        119    3768
 
PspOMI G'GGCC_C

Cuts at:    729     729 
   Size:       3887
 
PstI C_TGCA'G

Cuts at:    599     934    1619     599 
   Size:        335     685    2867

  Fragments arranged by size: 

                2867     685     335
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3887
 
PvuII CAG'CTG

Cuts at:    289     492     582    1312    1672     289 
   Size:        203      90     730     360    2504

  Fragments arranged by size: 

                2504     730     360     203      90
 
RsrII CG'GwC_CG

Cuts at:   2082    2082 
   Size:       3887
 
SacI G_AGCT'C

Cuts at:    901     901 
   Size:       3887
 
SalI G'TCGA_C

Cuts at:    924     924 
   Size:       3887
 
SanDI GG'GwC_CC

Cuts at:    257     257 
   Size:       3887
 
SapI GCTCTTCn'nnn_

Cuts at:   1910    2120    1910 
   Size:        210    3677
 
SbfI CC_TGCA'GG

Cuts at:    934     934 
   Size:       3887
 
ScaI AGT'ACT

Cuts at:   3404    3404 
   Size:       3887
 
SfcI C'TryA_G

Cuts at:     33     595     821     930    1615    2608    2799      33 
   Size:        562     226     109     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     562     226     191     109
 
SfoI GGC'GCC

Cuts at:   1319    1567    1319 
   Size:        248    3639
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3887
 
SmaI CCC'GGG

Cuts at:    909     909 
   Size:       3887
 
SmlI C'TyrA_G

Cuts at:   1042    1957    2449    2711    1042 
   Size:        915     492     262    2218

  Fragments arranged by size: 

                2218     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3887
 
SphI G_CATG'C

Cuts at:    940    1440     940 
   Size:        500    3387
 
StyI C'CwwG_G

Cuts at:    674     768    1021    1375     674 
   Size:         94     253     354    3186

  Fragments arranged by size: 

                3186     354     253      94
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    148     267     484     847    1470    1603    2062    2271     148 
   Size:        119     217     363     623     133     459     209    1764

  Fragments arranged by size: 

                1764     623     459     363     217     209     133     119
            
 
TatI w'GTAC_w

Cuts at:   3402    3402 
   Size:       3887
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3404    3436    3404 
   Size:         32    3855
 
Tth111I GACn'n_nGTC

Cuts at:   1684    1684 
   Size:       3887
 
XbaI T'CTAG_A

Cuts at:    918     918 
   Size:       3887
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    223     579     721    1382     223 
   Size:        356     142     661    2728

  Fragments arranged by size: 

                2728     661     356     142
 
XmaI C'CCGG_G

Cuts at:    907     907 
   Size:       3887
 
XmnI GAAnn'nnTTC

Cuts at:    316     316 
   Size:       3887
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI     BcgI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI     BtgI
    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI
    PflMI     PfoI     PmeI     PpiI    PpuMI   PspOMI     PstI     PvuI
    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AlfI     AscI    AvrII
     BaeI    BbvCI    BfrBI    BmgBI     BmtI    BsiWI     BsmI    BsmBI
    BsrGI   BssHII   BstEII  BstZ17I   Bsu36I     ClaI    CspCI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PmlI    PshAI     PsiI    PspXI
     PsrI    SacII    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     ZraI
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