Restriction map for pF1KB7325
##################################
# NO CUT ENZYMES (   63 enzymes) #
##################################

AatII       Acc65I      AflII       AhdI        AscI        
Asp700I     Asp718I     AspEI       AviII       AvrII       
BbeI        BciVI       BfrI        BlnI        BsaBI       
BsiWI       BsmBI       BsmI        BspLU11I    BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
DraIII      Eam1105I    EcoO65I     EcoT22I     FseI        
FspI        HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MunI        NarI        
NdeI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PmeI        PvuI        SacI        
SacII       SbfI        SexAI       SfoI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SwaI        
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccIII         45  TCCGGA               T^CCGGA
AclI          596  AACGTT               AA^CGTT
AflIII       3420  ACGTGT               A^C[GA][CT]GT
AgeI          141  ACCGGT               A^CCGGT
AocI          728  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      2147  AGCGCT               AGC^GCT
AseI         3828  ATTAAT               AT^TAAT
AsnI         3828  ATTAAT               AT^TAAT
AspI         2461  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI         1309  GGCACC               G^G[CT][GA]CC
BbrPI         385  CACGTG               CAC^GTG
BsaAI         385  CACGTG               [CT]AC^GT[GA]
BsaI         1152  GGTCTC               GGTCTC(1/5)
BseAI          45  TCCGGA               T^CCGGA
Bsp1407I     4910  TGTACA               T^GTACA
BspEI          45  TCCGGA               T^CCGGA
BsrGI        4910  TGTACA               T^GTACA
BstAPI       3033  GCATGAAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        1115  CCATCGTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        728  CCTCAGG              CC^T[ACGT]AGG
CpoI          138  CGGACCG              CG^G[AT]CCG
DrdI         1911  GACACTGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     2147  AGCGCT               AGC^GCT
Eco81I        728  CCTCAGG              CC^T[ACGT]AGG
EcoRI        1036  GAATTC               G^AATTC
EcoRV        4140  GATATC               GAT^ATC
HindIII      4505  AAGCTT               A^AGCTT
MroI           45  TCCGGA               T^CCGGA
NheI         4608  GCTAGC               G^CTAGC
PflFI        2461  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI         141  ACCGGT               A^CCGGT
PmaCI         385  CACGTG               CAC^GTG
PmlI          385  CACGTG               CAC^GTG
PshAI        4473  GACTAAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        3828  ATTAAT               AT^TAAT
Psp1406I      596  AACGTT               AA^CGTT
RsrII         138  CGGACCG              CG^G[AT]CCG
SalI         3764  GTCGAC               G^TCGAC
SfiI           84  GGCCCTGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI         2910  GCATGC               GCATG^C
SspBI        4910  TGTACA               T^GTACA
SspI         4749  AATATT               AAT^ATT
Tth111I      2461  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         3458  CCAAAGAACTTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

Alw44I        838  GTGCAC               G^TGCAC
             2383  GTGCAC              
ApaI           17  GGGCCC               GGGCC^C
             2017  GGGCCC              
ApaLI         838  GTGCAC               G^TGCAC
             2383  GTGCAC              
BamHI        3356  GGATCC               G^GATCC
             3641  GGATCC              
BbvCI        1470  CCTCAGC              CCTCAGC(-5/-2)
             2112  CCTCAGC             
BcgI         4512  CGATAATCTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1197  GCATCAGCTTCG        
BclI          825  TGATCA               T^GATCA
             3935  TGATCA              
BglI           85  GCCCTGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1175  GCCACTCAGGC         
BglII        3098  AGATCT               A^GATCT
             3804  AGATCT              
BlpI          586  GCTAAGC              GC^T[ACGT]AGC
             1219  GCTCAGC             
Bpu1102I      586  GCTAAGC              GC^T[ACGT]AGC
             1219  GCTCAGC             
BsaWI          45  TCCGGA               [AT]^CCGG[AT]
              141  ACCGGT              
BspDI        3127  ATCGAT               AT^CGAT
             3446  ATCGAT              
BspHI         435  TCATGA               T^CATGA
             4335  TCATGA              
BsrBI        1969  GAGCGG               CCGCTC(-3/-3)
             1978  GAGCGG              
BsrDI        1087  GCAATG               GCAATG(2/0)
             2330  GCAATG              
BssSI          73  CACGAG               CACGAG(-5/-1)
             1975  CACGAG              
CelII         586  GCTAAGC              GC^T[ACGT]AGC
             1219  GCTCAGC             
ClaI         3127  ATCGAT               AT^CGAT
             3446  ATCGAT              
DraI         3223  TTTAAA               TTT^AAA
             3399  TTTAAA              
FbaI          825  TGATCA               T^GATCA
             3935  TGATCA              
HincII       2554  GTCAAC               GT[CT]^[GA]AC
             3764  GTCGAC              
HindII       2554  GTCAAC               GT[CT]^[GA]AC
             3764  GTCGAC              
NcoI         2819  CCATGG               C^CATGG
             4575  CCATGG              
NspI         2910  GCATGC               [GA]CATG^[CT]
             3195  GCATGT              
PflMI        2763  CCAGAAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3987  CCAGTTTGTGG         
PstI         4326  CTGCAG               CTGCA^G
             4459  CTGCAG              
RcaI          435  TCATGA               T^CATGA
             4335  TCATGA              
SapI          502  GAAGAGC              GCTCTTC(1/4)
             2418  GAAGAGC             
ScaI         2408  AGTACT               AGT^ACT
             4322  AGTACT              
SmaI           22  CCCGGG               CCC^GGG
             2181  CCCGGG              
SmlI          182  CTCAAG               C^T[CT][GA]AG
             2986  CTCAAG              
Van91I       2763  CCAGAAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3987  CCAGTTTGTGG         
XmaCI          22  CCCGGG               C^CCGGG
             2181  CCCGGG              
XmaI           22  CCCGGG               C^CCGGG
             2181  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  116 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AluI        AlwI        AlwNI       ApoI        
AspHI       AvaI        AvaII       BalI        BanII       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaHI       BsaJI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EagI        EarI        EclXI       Eco52I      
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       PleI        PpuMI       PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       
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