Restriction map for pF1KB6552
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        AhdI        AocI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       BamHI       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BglII       BlpI        Bpu1102I    
BsaAI       BsaBI       BseAI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspEI       BspHI       BspLU11I    
BsrDI       BsrFI       BsrGI       BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
CelII       Cfr10I      CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRV       
EcoT22I     FbaI        FseI        FspI        HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MamI        MfeI        MluI        MroI        MunI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        TfiI        Tth111I     XbaI        
XhoI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI          319  GTCTAC               GT^[AC][GT]AC
AcsI          466  GAATTC               [GA]^AATT[CT]
AcyI          652  GACGCC               G[GA]^CG[CT]C
ApoI          466  GAATTC               [GA]^AATT[CT]
AvaI         1038  CTCGGG               C^[CT]CG[GA]G
AvrII         183  CCTAGG               C^CTAGG
BalI         1004  TGGCCA               TGG^CCA
BanI          103  GGCACC               G^G[CT][GA]CC
BbsI         1093  GTCTTC               GAAGAC(2/6)
BglI          839  GCCAGGCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI          183  CCTAGG               C^CTAGG
BpuAI        1093  GTCTTC               GAAGAC(2/6)
BsaHI         652  GACGCC               G[GA]^CG[CT]C
BsaI         1107  GGTCTC               GGTCTC(1/5)
BsaWI        1104  TCCGGT               [AT]^CCGG[AT]
BsiEI         856  CGGTCG               CG[GA][CT]^CG
BsoBI        1038  CTCGGG               C^[CT]CG[GA]G
BspDI         796  ATCGAT               AT^CGAT
BspMI        1148  ACCTGC               ACCTGC(4/8)
BssHII        339  GCGCGC               G^CGCGC
ClaI          796  ATCGAT               AT^CGAT
DdeI          230  CTAAG                C^T[ACGT]AG
EcoNI         351  CCTCCGCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         466  GAATTC               G^AATTC
HgaI          652  GACGC                GACGC(5/10)
Hin1I         652  GACGCC               G[GA]^CG[CT]C
HincII        193  GTCAAC               GT[CT]^[GA]AC
HindII        193  GTCAAC               GT[CT]^[GA]AC
HinfI         991  GAGTC                G^A[ACGT]TC
MluNI        1004  TGGCCA               TGG^CCA
MscI         1004  TGGCCA               TGG^CCA
MseI          929  TTAA                 T^TAA
NheI         1030  GCTAGC               G^CTAGC
NspI          644  ACATGC               [GA]CATG^[CT]
PflMI         996  CCAGGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          991  GAGTC                GAGTC(4/5)
StuI          914  AGGCCT               AGG^CCT
Tru9I         929  TTAA                 T^TAA
Tsp45I          4  GTGAC                ^GT[GC]AC
Tsp509I       467  AATT                 ^AATT
Van91I        996  CCAGGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

Alw44I        832  GTGCAC               G^TGCAC
             1120  GTGCAC              
AlwI          255  GATCC                GGATC(4/5)
              594  GATCC               
ApaLI         832  GTGCAC               G^TGCAC
             1120  GTGCAC              
BanII         234  GGGCTC               G[GA]GC[CT]^C
              529  GAGCCC              
BfaI          184  CTAG                 C^TAG
             1031  CTAG                
BpmI          247  CTGGAG               CTGGAG(16/14)
              907  CTGGAG              
BsgI         1150  CTGCAC               GTGCAG(16/14)
              382  GTGCAG              
BsrBI         120  GAGCGG               CCGCTC(-3/-3)
             1054  GAGCGG              
BsrI          518  CCAGT                ACTGG(1/-1)
              736  CCAGT               
BstXI         482  CCAAGGCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              866  CCATCTGCCTGG        
BstYI         254  AGATCC               [GA]^GATC[CT]
              593  AGATCC              
DpnII         255  GATC                 ^GATC
              594  GATC                
EaeI          860  CGGCCA               [CT]^GGCC[GA]
             1004  TGGCCA              
HaeII         387  GGCGCT               [GA]GCGC^[CT]
              935  AGCGCC              
MaeI          184  CTAG                 C^TAG
             1031  CTAG                
MaeII         197  ACGT                 A^CGT
             1009  ACGT                
MaeIII          4  GTGAC                ^GT[ACGT]AC
              206  GTAAC               
MboI          255  GATC                 ^GATC
              594  GATC                
MflI          254  AGATCC               [GA]^GATC[CT]
              593  AGATCC              
MslI          210  CACCATCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              732  CATCCCAGTG          
NdeII         255  GATC                 ^GATC
              594  GATC                
PvuII         976  CAGCTG               CAG^CTG
             1176  CAGCTG              
Sau3AI        255  GATC                 ^GATC
              594  GATC                
XcmI          524  CCACAGAGCCCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              863  CCACCATCTGCCTGG     
XhoII         254  AGATCC               [GA]^GATC[CT]
              593  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwNI       
AspHI       AvaII       BbvI        BcnI        BmyI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BssKI       BstAPI      
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DraII       DsaI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HpaII       HphI        
ItaI        MboII       MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NlaIII      
NlaIV       PpuMI       PstI        RsaI        Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
TseI        TspRI       TthHB8I     
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