Restriction map for pF1KB6344
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# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AflIII      
AgeI        AhdI        AocI        ApaI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BamHI       
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaI        BsiEI       Bsp1407I    
BspLU11I    BspMI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      CelII       CpoI        
DraI        EaeI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MaeIII      MamI        MfeI        MluI        MluNI       
MscI        MseI        MunI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SexAI       SfiI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tru9I       Tsp45I      Tth111I     XbaI        XhoI        
XmnI        


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# ONE CUT ENZYMES (   71 enzymes) #
###################################

AccI          619  GTCTAC               GT^[AC][GT]AC
AccII         676  CGCG                 CG^CG
AccIII        305  TCCGGA               T^CCGGA
AcsI          547  AAATTC               [GA]^AATT[CT]
AcyI          141  GGCGCC               G[GA]^CG[CT]C
Alw44I        249  GTGCAC               G^TGCAC
Aor51HI       374  AGCGCT               AGC^GCT
ApaLI         249  GTGCAC               G^TGCAC
ApoI          547  AAATTC               [GA]^AATT[CT]
AspHI         249  GTGCAC               G[AT]GC[AT]^C
AvaI          461  CCCGGG               C^[CT]CG[GA]G
BanII           4  GGGCTC               G[GA]GC[CT]^C
BbeI          141  GGCGCC               GGCGC^C
BbsI          167  GTCTTC               GAAGAC(2/6)
BfaI          110  CTAG                 C^TAG
BglI           19  GCCACCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         167  GTCTTC               GAAGAC(2/6)
BsaHI         141  GGCGCC               G[GA]^CG[CT]C
BsaWI         305  TCCGGA               [AT]^CCGG[AT]
BseAI         305  TCCGGA               T^CCGGA
BseRI         133  CTCCTC               GAGGAG(10/8)
BsgI          781  GTGCAG               GTGCAG(16/14)
BsiHKAI       249  GTGCAC               G[AT]GC[AT]^C
BsiWI         452  CGTACG               C^GTACG
BsmBI         859  GAGACG               CGTCTC(1/5)
BsmI          507  GAATGC               GAATGC(1/-1)
BsoBI         461  CCCGGG               C^[CT]CG[GA]G
BspDI         415  ATCGAT               AT^CGAT
BspEI         305  TCCGGA               T^CCGGA
BspHI         737  TCATGA               T^CATGA
BsrBI         708  CCGCTC               CCGCTC(-3/-3)
BsrFI         220  GCCGGC               [GA]^CCGG[CT]
BsrI          849  ACTGG                ACTGG(1/-1)
BstUI         676  CGCG                 CG^CG
BstYI         317  AGATCC               [GA]^GATC[CT]
Cfr10I        220  GCCGGC               [GA]^CCGG[CT]
ClaI          415  ATCGAT               AT^CGAT
DraII         299  AGGACCT              [GA]G^G[ACGT]CC[CT]
DraIII        499  CACGTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          610  GACCTCAGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          436  CTCTTC               CTCTTC(1/4)
Eco47III      374  AGCGCT               AGC^GCT
EcoNI         805  CCTAACTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      299  AGGACCT              [GA]G^G[ACGT]CC[CT]
FauI          855  GCGGG                CCCGC(4/6)
HgaI          756  GACGC                GACGC(5/10)
Hin1I         141  GGCGCC               G[GA]^CG[CT]C
HphI          494  TCACC                GGTGA(8/7)
KasI          141  GGCGCC               G^GCGCC
Ksp632I       436  CTCTTC               CTCTTC(1/4)
MaeI          110  CTAG                 C^TAG
MaeII         500  ACGT                 A^CGT
MflI          317  AGATCC               [GA]^GATC[CT]
MroI          305  TCCGGA               T^CCGGA
MslI          412  CATATCGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          676  CGCG                 CG^CG
NaeI          220  GCCGGC               GCC^GGC
NarI          141  GGCGCC               GG^CGCC
NgoMIV        220  GCCGGC               G^CCGGC
PleI          283  GACTC                GAGTC(4/5)
PpuMI         299  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII         484  CAGCTG               CAG^CTG
RcaI          737  TCATGA               T^CATGA
ScaI          572  AGTACT               AGT^ACT
SfoI          141  GGCGCC               GGC^GCC
SmaI          461  CCCGGG               CCC^GGG
TfiI          491  GAATC                G^A[AT]TC
Tsp509I       548  AATT                 ^AATT
XhoII         317  AGATCC               [GA]^GATC[CT]
XmaCI         461  CCCGGG               C^CCGGG
XmaI          461  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AlwNI         159  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
              832  CAGGTTCTG           
AvaII         300  GGACC                G^G[AT]CC
              387  GGTCC               
BanI          141  GGCGCC               G^G[CT][GA]CC
              541  GGTGCC              
BcnI          461  CCCGG                CC^[GC]GG
              462  CCGGG               
BmyI            4  GGGCTC               G[AGT]GC[ACT]^C
              249  GTGCAC              
BsmFI         464  GGGAC                GGGAC(10/14)
              564  GTCCC               
Bsp1286I        4  GGGCTC               G[AGT]GC[ACT]^C
              249  GTGCAC              
CfoI          142  GCGC                 GCG^C
              375  GCGC                
HaeII         141  GGCGCC               [GA]GCGC^[CT]
              374  AGCGCT              
HhaI          142  GCGC                 GCG^C
              375  GCGC                
HinP1I        142  GCGC                 G^CGC
              375  GCGC                
HinfI         283  GACTC                G^A[ACGT]TC
              491  GAATC               
MspA1I        484  CAGCTG               C[AC]G^C[GT]G
              512  CCGCTG              
NciI          461  CCCGG                CC^[GC]GG
              462  CCGGG               
NcoI          321  CCATGG               C^CATGG
              695  CCATGG              
PflMI         273  CCACAGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              831  CCAGGTTCTGG         
Van91I        273  CCACAGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              831  CCAGGTTCTGG         
XcmI          431  CCAGCCTCTTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              716  CCACACTTGTCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BbvI        
BpmI        BsaJI       BsiYI       BslI        BsmAI       
BssKI       BstAPI      BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       DsaI        EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HpaII       ItaI        MboI        MboII       MnlI        
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
TseI        TspRI       TthHB8I     
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