Restriction map for pF1KE0347
##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        AhdI        AocI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BbrPI       
BbvCI       BciVI       BclI        BfrI        BglII       
BlnI        BsaWI       BseAI       BsiWI       BsmI        
Bsp1407I    BspDI       BspEI       BsrBI       BsrGI       
BssHII      Bst1107I    BstEII      BstPI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        Eam1105I    
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HpaI        KpnI        
KspI        MfeI        MluI        MroI        MunI        
NdeI        NheI        NotI        NsiI        PacI        
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        ScaI        SnaBI       SpeI        
SspBI       SwaI        Tth111I     XbaI        XcmI        
XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AcsI         2020  GAATTT               [GA]^AATT[CT]
AlwNI        2479  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      1589  AGCGCT               AGC^GCT
ApoI         2020  GAATTT               [GA]^AATT[CT]
BalI          866  TGGCCA               TGG^CCA
BbeI         1610  GGCGCC               GGCGC^C
BglI          377  GCCGGCAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          189  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I      189  GCTAAGC              GC^T[ACGT]AGC
BsaAI        1870  TACGTG               [CT]AC^GT[GA]
BsaI          813  GGTCTC               GGTCTC(1/5)
BspHI        2375  TCATGA               T^CATGA
BsrDI        1830  CATTGC               GCAATG(2/0)
BstAPI       1433  GCACTGTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        2017  TTCGAA               TT^CGAA
BstXI         783  CCAGTGGTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        2420  GGATCT               [GA]^GATC[CT]
CelII         189  GCTAAGC              GC^T[ACGT]AGC
DraIII       1434  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          247  GACACCCCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1589  AGCGCT               AGC^GCT
HincII         67  GTCAAC               GT[CT]^[GA]AC
HindII         67  GTCAAC               GT[CT]^[GA]AC
HindIII      1549  AAGCTT               A^AGCTT
KasI         1610  GGCGCC               G^GCGCC
MflI         2420  GGATCT               [GA]^GATC[CT]
MluNI         866  TGGCCA               TGG^CCA
MscI          866  TGGCCA               TGG^CCA
NarI         1610  GGCGCC               GG^CGCC
NspV         2017  TTCGAA               TT^CGAA
PaeR7I       2260  CTCGAG               C^TCGAG
PflMI         909  CCATGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI         2375  TCATGA               T^CATGA
SacI         2410  GAGCTC               GAGCT^C
SbfI         1635  CCTGCAGG             CCTGCA^GG
SexAI         427  ACCAGGT              A^CC[AT]GGT
SfiI          376  GGCCGGCAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1610  GGCGCC               GGC^GCC
SfuI         2017  TTCGAA               TT^CGAA
SgrAI          39  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI         2218  CCCGGG               CCC^GGG
SphI          770  GCATGC               GCATG^C
Sse8387I     1635  CCTGCAGG             CCTGCA^GG
SspI         1903  AATATT               AAT^ATT
Tsp45I       1655  GTGAC                ^GT[GC]AC
Van91I        909  CCATGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         2260  CTCGAG               C^TCGAG
XhoII        2420  GGATCT               [GA]^GATC[CT]
XmaCI        2218  CCCGGG               C^CCGGG
XmaI         2218  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccII         158  CGCG                 CG^CG
             1752  CGCG                
Alw44I       2403  GTGCAC               G^TGCAC
             2437  GTGCAC              
ApaLI        2403  GTGCAC               G^TGCAC
             2437  GTGCAC              
BanI         1610  GGCGCC               G^G[CT][GA]CC
             2470  GGTGCC              
BbsI           52  GAAGAC               GAAGAC(2/6)
             1139  GAAGAC              
BpuAI          52  GAAGAC               GAAGAC(2/6)
             1139  GAAGAC              
BsaBI        1234  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1414  GATGGCTATC          
BseRI          31  GAGGAG               GAGGAG(10/8)
             1822  GAGGAG              
BsgI         1431  CTGCAC               GTGCAG(16/14)
             1091  GTGCAG              
BsiEI        1538  CGGCCG               CG[GA][CT]^CG
             2068  CGGCCG              
BsmBI         885  CGTCTC               CGTCTC(1/5)
              438  GAGACG              
BspLU11I     1400  ACATGT               A^CATGT
             1574  ACATGT              
BspMI        2030  ACCTGC               ACCTGC(4/8)
              609  GCAGGT              
BstUI         158  CGCG                 CG^CG
             1752  CGCG                
EagI         1538  CGGCCG               C^GGCCG
             2068  CGGCCG              
EclXI        1538  CGGCCG               C^GGCCG
             2068  CGGCCG              
Eco52I       1538  CGGCCG               C^GGCCG
             2068  CGGCCG              
EcoNI         570  CCTAGCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1989  CCTGTATGAGG         
MaeIII       1571  GTTAC                ^GT[ACGT]AC
             1655  GTGAC               
MamI         1234  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1414  GATGGCTATC          
MvnI          158  CGCG                 CG^CG
             1752  CGCG                
NcoI          469  CCATGG               C^CATGG
              722  CCATGG              
NruI          157  TCGCGA               TCG^CGA
             1751  TCGCGA              
SapI          267  GCTCTTC              GCTCTTC(1/4)
             2010  GCTCTTC             
TfiI         1111  GAATC                G^A[AT]TC
             1971  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   97 enzymes) #
#########################################

AciI        AcyI        AfaI        AflIII      AluI        
AlwI        ApaI        AspHI       AvaI        AvaII       
BanII       BbvI        BcgI        BcnI        BfaI        
BmyI        BpmI        BsaHI       BsaJI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrFI       BsrI        BssKI       BssSI       
BstF5I      BstNI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MboI        MboII       MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NaeI        NciI        NdeII       NgoMIV      
NlaIII      NlaIV       NspI        PleI        PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StuI        
StyI        TaqI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I     
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