Restriction map for pF1KE0347
 (Circular) (Six-base) MAPSORT of: pF1KE0347.seq  Check: 8982  from: 1  to: 5672



pF1KE0347  5672 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    708    2147     708 
   Size:       1439    4233
 
AccI GT'mk_AC

Cuts at:   2710    2710 
   Size:       5672
 
Acc65I G'GTAC_C

Cuts at:   2688    2688 
   Size:       5672
 
AclI AA'CG_TT

Cuts at:   5512    5512 
   Size:       5672
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1114    1355    1645    1940    2665    3497    3929    4676    4944 
   Size:        241     290     295     725     832     432     747     268

Cuts at:   4944    1114 
   Size:       1842

  Fragments arranged by size: 

                1842     832     747     725     432     295     290     268
                 241
 
AfeI AGC'GCT

Cuts at:   1699    1699 
   Size:       5672
 
AflIII A'CryG_T

Cuts at:   1508    1682    2558    3024    4128    5105    1508 
   Size:        174     876     466    1104     977    2075

  Fragments arranged by size: 

                2075    1104     977     876     466     174
 
AgeI A'CCGG_T

Cuts at:   4060    4060 
   Size:       5672
 
AleI CACnn'nnGTG

Cuts at:   2557    2557 
   Size:       5672
 
AlwNI CAG_nnn'CTG

Cuts at:   2592    2977    4544    2592 
   Size:        385    1567    3720

  Fragments arranged by size: 

                3720    1567     385
 
ApaI G_GGCC'C

Cuts at:   1116    2029    2304    1116 
   Size:        913     275    4484

  Fragments arranged by size: 

                4484     913     275
 
ApaLI G'TGCA_C

Cuts at:   2511    2545    4442    2511 
   Size:         34    1897    3741

  Fragments arranged by size: 

                3741    1897      34
 
ApoI r'AATT_y

Cuts at:   2128    2676    5025    5281    2128 
   Size:        548    2349     256    2519

  Fragments arranged by size: 

                2519    2349     548     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5672
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5672
 
AvaI C'yCGr_G

Cuts at:    479    2326    2368    2692     479 
   Size:       1847      42     324    3459

  Fragments arranged by size: 

                3459    1847     324      42
 
BamHI G'GATC_C

Cuts at:   2697    2697 
   Size:       5672
 
BanI G'GyrC_C

Cuts at:   1718    2578    2688    3102    3350    3385    1718 
   Size:        860     110     414     248      35    4005

  Fragments arranged by size: 

                4005     860     414     248     110      35
 
BanII G_rGCy'C

Cuts at:   1116    1469    1670    2029    2304    2522    2686    3716    1116 
   Size:        353     201     359     275     218     164    1030    3072

  Fragments arranged by size: 

                3072    1030     359     353     275     218     201     164
            
 
BbeI G_GCGC'C

Cuts at:   1722    3106    3354    1722 
   Size:       1384     248    4040

  Fragments arranged by size: 

                4040    1384     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    167    1254     167 
   Size:       1087    4585
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    545     579     907     941    2502    2536     545 
   Size:         34     328      34    1561      34    3681

  Fragments arranged by size: 

                3681    1561     328      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3570    4331    5672    3570 
   Size:        761    1341    3570

  Fragments arranged by size: 

                3570    1341     761
 
BclI T'GATC_A

Cuts at:   3192    3192 
   Size:       5672
 
BglI GCCn_nnn'nGGC

Cuts at:    491     491 
   Size:       5672
 
BglII A'GATC_T

Cuts at:   3187    5224    3187 
   Size:       2037    3635
 
BlpI GC'TnA_GC

Cuts at:    298    2784     298 
   Size:       2486    3186
 
Bme1580I G_kGCm'C

Cuts at:   1116    1636    2029    2304    2515    2549    2583    3297    3390 
   Size:        520     393     275     211      34      34     714      93

Cuts at:   3390    4446    1116 
   Size:       1056    2342

  Fragments arranged by size: 

                2342    1056     714     520     393     275     211      93
                  34      34
 
BmgBI CAC'GTC

Cuts at:   1989    2559    1989 
   Size:        570    5102
 
BmrI ACTGGGnnnn_n'

Cuts at:    355     955    1145    2884    2965    3300    5444     355 
   Size:        600     190    1739      81     335    2144     583

  Fragments arranged by size: 

                2144    1739     600     583     335     190      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4039    4039 
   Size:       5672
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    241     426    1045    1091    1639    1692    2269    2628     241 
   Size:        185     619      46     548      53     577     359    3285

  Fragments arranged by size: 

                3285     619     577     548     359     185      53      46
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1288    1516    2848    3727    4219    4517    1288 
   Size:        228    1332     879     492     298    2443

  Fragments arranged by size: 

                2443    1332     879     492     298     228
 
BsaI GGTCTCn'nnnn_

Cuts at:    927    5376     927 
   Size:       4449    1223
 
BsaAI yAC'GTr

Cuts at:   1980    3025    1980 
   Size:       1045    4627
 
BsaBI GATnn'nnATC

Cuts at:      6    1346    1526    3186    5223       6 
   Size:       1340     180    1660    2037     455

  Fragments arranged by size: 

                2037    1660    1340     455     180
 
BsaHI Gr'CG_yC

Cuts at:    146     646    1709    1719    3103    3351    4053    5549     146 
   Size:        500    1063      10    1384     248     702    1496     269

  Fragments arranged by size: 

                1496    1384    1063     702     500     269     248      10
            
 
BsaWI w'CCGG_w

Cuts at:   2861    3382    4060    4334    4481    4892    2861 
   Size:        521     678     274     147     411    3641

  Fragments arranged by size: 

                3641     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    184     214     424     454     184 
   Size:         30     210      30    5402

  Fragments arranged by size: 

                5402     210      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    154    1945     154 
   Size:       1791    3881
 
BseYI C'CCAG_C

Cuts at:    408     592    1060    2595    3097    3855    4432    4928     408 
   Size:        184     468    1535     502     758     577     496    1152

  Fragments arranged by size: 

                1535    1152     758     577     502     496     468     184
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1220    1524    1220 
   Size:        304    5368
 
BsiEI CG_ry'CG

Cuts at:    104    1649    2179    3214    3260    4468     104 
   Size:       1545     530    1035      46    1208    1308

  Fragments arranged by size: 

                1545    1308    1208    1035     530      46
 
BsiHKAI G_wGCw'C

Cuts at:   2515    2522    2549    2686    3464    3654    4446    2515 
   Size:          7      27     137     778     190     792    3741

  Fragments arranged by size: 

                3741     792     778     190     137      27       7
 
BsmBI CGTCTCn'nnnn_

Cuts at:    540     999     540 
   Size:        459    5213
 
Bsp1286I G_dGCh'C

Cuts at:   1116    1469    1636    1670    2029    2304    2515    2522    2549 
   Size:        353     167      34     359     275     211       7      27

Cuts at:   2549    2583    2686    3297    3390    3464    3654    3716    4446 
   Size:         34     103     611      93      74     190      62     730

Cuts at:   4446    1116 
   Size:       2342

  Fragments arranged by size: 

                2342     730     611     359     353     275     211     190
                 167     103      93      74      62      34      34      27
                   7
 
BspEI T'CCGG_A

Cuts at:   4892    4892 
   Size:       5672
 
BspHI T'CATG_A

Cuts at:   2483    4848    2483 
   Size:       2365    3307
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    708    2147    2722    2936    3238    3619     708 
   Size:       1439     575     214     302     381    2761

  Fragments arranged by size: 

                2761    1439     575     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    3964    4018    5502    5667      50 
   Size:       3914      54    1484     165      55

  Fragments arranged by size: 

                3914    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1937    3584    1937 
   Size:       1647    4025
 
BsrFI r'CCGG_y

Cuts at:    148     485    1595    2114    2174    3670    3851    4060     148 
   Size:        337    1110     519      60    1496     181     209    1760

  Fragments arranged by size: 

                1760    1496    1110     519     337     209     181      60
            
 
BssSI C'ACGA_G

Cuts at:    790    1584    1960    3943    4301     790 
   Size:        794     376    1983     358    2161

  Fragments arranged by size: 

                2161    1983     794     376     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1547    3049    1547 
   Size:       1502    4170
 
BstBI TT'CG_AA

Cuts at:   2126    4033    2126 
   Size:       1907    3765
 
BstXI CCAn_nnnn'nTGG

Cuts at:    898     898 
   Size:       5672
 
BstYI r'GATC_y

Cuts at:      1    2528    2697    3164    3187    3522    3768    4780    4866 
   Size:       2527     169     467      23     335     246    1012      86

Cuts at:   4866    4878    5224       1 
   Size:         12     346     449

  Fragments arranged by size: 

                2527    1012     467     449     346     335     246     169
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    518     577     830    1116    2574     518 
   Size:         59     253     286    1458    3616

  Fragments arranged by size: 

                3616    1458     286     253      59
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    338     917    1842    3622    3803    3966     338 
   Size:        579     925    1780     181     163    2044

  Fragments arranged by size: 

                2044    1780     925     579     181     163
 
BtsI GCAGTG_nn'

Cuts at:   2915    2915 
   Size:       5672
 
DraI TTT'AAA

Cuts at:   2671    2671 
   Size:       5672
 
DraIII CAC_nnn'GTG

Cuts at:   1547    1547 
   Size:       5672
 
DrdI GACnn_nn'nnGTC

Cuts at:    361    3378    4236     361 
   Size:       3017     858    1797

  Fragments arranged by size: 

                3017    1797     858
 
EaeI y'GGCC_r

Cuts at:    580     974    1646    2176    3257    3431    3822    3849    5194 
   Size:        394     672     530    1081     174     391      27    1345

Cuts at:   5194     580 
   Size:       1058

  Fragments arranged by size: 

                1345    1081    1058     672     530     394     391     174
                  27
 
EagI C'GGCC_G

Cuts at:   1646    2176    3257    1646 
   Size:        530    1081    4061

  Fragments arranged by size: 

                4061    1081     530
 
EarI CTCTTCn'nnn_

Cuts at:    382    2125    2638    3695    3905     382 
   Size:       1743     513    1057     210    2149

  Fragments arranged by size: 

                2149    1743    1057     513     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2063    3880    4190    4336    5482    2063 
   Size:       1817     310     146    1146    2253

  Fragments arranged by size: 

                2253    1817    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   2520    2684    2520 
   Size:        164    5508
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    241     426    1045    1091    1114    1355    1639    1645    1692 
   Size:        185     619      46      23     241     284       6      47

Cuts at:   1692    1940    2269    2628    2665    3497    3929    4676    4944 
   Size:        248     329     359      37     832     432     747     268

Cuts at:   4944     241 
   Size:        969

  Fragments arranged by size: 

                 969     832     747     619     432     359     329     284
                 268     248     241     185      47      46      37      23
                   6
 
EcoNI CCTnn'n_nnAGG

Cuts at:    682    2101     682 
   Size:       1419    4253
 
EcoO109I rG'GnC_Cy

Cuts at:    182     539     786    1146    2025    2300    2603    2811     182 
   Size:        357     247     360     879     275     303     208    3043

  Fragments arranged by size: 

                3043     879     360     357     303     275     247     208
            
 
EcoRI G'AATT_C

Cuts at:   2676    2676 
   Size:       5672
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2670    3691    2670 
   Size:       1021    4651
 
FspI TGC'GCA

Cuts at:   3453    3453 
   Size:       5672
 
HaeII r_GCGC'y

Cuts at:   1302    1701    1722    3106    3354    4376    5355    1302 
   Size:        399      21    1384     248    1022     979    1619

  Fragments arranged by size: 

                1619    1384    1022     979     399     248      21
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    184     216    3514    3544    3546    3576     184 
   Size:         32    3298      30       2      30    2280

  Fragments arranged by size: 

                3298    2280      32      30      30       2
 
HincII GTy'rAC

Cuts at:    177    2711     177 
   Size:       2534    3138
 
HindIII A'AGCT_T

Cuts at:   1657    1657 
   Size:       5672
 
KasI G'GCGC_C

Cuts at:   1718    3102    3350    1718 
   Size:       1384     248    4040

  Fragments arranged by size: 

                4040    1384     248
 
KpnI G_GTAC'C

Cuts at:   2692    2692 
   Size:       5672
 
MluI A'CGCG_T

Cuts at:   5105    5105 
   Size:       5672
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    227    1085    1926    2588    2649    4343    4527     227 
   Size:        858     841     662      61    1694     184    1372

  Fragments arranged by size: 

                1694    1372     858     841     662     184      61
 
MscI TGG'CCA

Cuts at:    976    3433     976 
   Size:       2457    3215
 
MslI CAynn'nnrTG

Cuts at:    131     835    2557    3661    3788     131 
   Size:        704    1722    1104     127    2015

  Fragments arranged by size: 

                2015    1722    1104     704     127
 
MspA1I CmG'CkG

Cuts at:    412    1092    1258    1645    2783    3097    3457    4470    4715 
   Size:        680     166     387    1138     314     360    1013     245

Cuts at:   4715     412 
   Size:       1369

  Fragments arranged by size: 

                1369    1138    1013     680     387     360     314     245
                 166
 
NaeI GCC'GGC

Cuts at:    150     487    2116    3853     150 
   Size:        337    1629    1737    1969

  Fragments arranged by size: 

                1969    1737    1629     337
 
NarI GG'CG_CC

Cuts at:   1719    3103    3351    1719 
   Size:       1384     248    4040

  Fragments arranged by size: 

                4040    1384     248
 
NcoI C'CATG_G

Cuts at:    577     830     577 
   Size:        253    5419
 
NgoMIV G'CCGG_C

Cuts at:    148     485    2114    3851     148 
   Size:        337    1629    1737    1969

  Fragments arranged by size: 

                1969    1737    1629     337
 
NruI TCG'CGA

Cuts at:    267    1861     267 
   Size:       1594    4078
 
NspI r_CATG'y

Cuts at:    882    1481    1512    1686    2663    2725    3225    4132     882 
   Size:        599      31     174     977      62     500     907    2422

  Fragments arranged by size: 

                2422     977     907     599     500     174      62      31
            
 
PciI A'CATG_T

Cuts at:   1508    1682    4128    1508 
   Size:        174    2446    3052

  Fragments arranged by size: 

                3052    2446     174
 
PflMI CCAn_nnn'nTGG

Cuts at:   1023    1023 
   Size:       5672
 
PfoI T'CCnGG_A

Cuts at:    218     380    2246    2605     218 
   Size:        162    1866     359    3285

  Fragments arranged by size: 

                3285    1866     359     162
 
PmeI GTTT'AAAC

Cuts at:   2671    2671 
   Size:       5672
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4811    4843    4811 
   Size:         32    5640
 
PpuMI rG'GwC_Cy

Cuts at:    539     786    2603     539 
   Size:        247    1817    3608

  Fragments arranged by size: 

                3608    1817     247
 
PspOMI G'GGCC_C

Cuts at:   1112    2025    2300    1112 
   Size:        913     275    4484

  Fragments arranged by size: 

                4484     913     275
 
PspXI vC'TCGA_Gb

Cuts at:   2368    2368 
   Size:       5672
 
PstI C_TGCA'G

Cuts at:    190     930    1748    2378    2719    3404     190 
   Size:        740     818     630     341     685    2458

  Fragments arranged by size: 

                2458     818     740     685     630     341
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5672
 
PvuII CAG'CTG

Cuts at:    412    1092    1258    3097    3457     412 
   Size:        680     166    1839     360    2627

  Fragments arranged by size: 

                2627    1839     680     360     166
 
RsrII CG'GwC_CG

Cuts at:   3867    3867 
   Size:       5672
 
SacI G_AGCT'C

Cuts at:   2522    2686    2522 
   Size:        164    5508
 
SalI G'TCGA_C

Cuts at:   2709    2709 
   Size:       5672
 
SapI GCTCTTCn'nnn_

Cuts at:    382    2125    3695    3905     382 
   Size:       1743    1570     210    2149

  Fragments arranged by size: 

                2149    1743    1570     210
 
SbfI CC_TGCA'GG

Cuts at:   1748    2719    1748 
   Size:        971    4701
 
ScaI AGT'ACT

Cuts at:   5189    5189 
   Size:       5672
 
SexAI A'CCwGG_T

Cuts at:    535     535 
   Size:       5672
 
SfcI C'TryA_G

Cuts at:     33     186     926    1744    2374    2715    3400    4393    4584 
   Size:        153     740     818     630     341     685     993     191

Cuts at:   4584      33 
   Size:       1121

  Fragments arranged by size: 

                1121     993     818     740     685     630     341     191
                 153
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    491     491 
   Size:       5672
 
SfoI GGC'GCC

Cuts at:   1720    3104    3352    1720 
   Size:       1384     248    4040

  Fragments arranged by size: 

                4040    1384     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5672
 
SgrAI Cr'CCGG_yG

Cuts at:    148     148 
   Size:       5672
 
SmaI CCC'GGG

Cuts at:   2328    2694    2328 
   Size:        366    5306
 
SmlI C'TyrA_G

Cuts at:   1303    1495    2368    2827    3742    4234    4496    1303 
   Size:        192     873     459     915     492     262    2479

  Fragments arranged by size: 

                2479     915     873     492     459     262     192
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5672
 
SphI G_CATG'C

Cuts at:    882    2725    3225     882 
   Size:       1843     500    3329

  Fragments arranged by size: 

                3329    1843     500
 
SspI AAT'ATT

Cuts at:   2013    2013 
   Size:       5672
 
StuI AGG'CCT

Cuts at:    462    1750    2096     462 
   Size:       1288     346    4038

  Fragments arranged by size: 

                4038    1288     346
 
StyI C'CwwG_G

Cuts at:    577     830    2159    2806    3160     577 
   Size:        253    1329     647     354    3089

  Fragments arranged by size: 

                3089    1329     647     354     253
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1308    1349    3255    3388    3847    4056    1308 
   Size:         41    1906     133     459     209    2924

  Fragments arranged by size: 

                2924    1906     459     209     133      41
 
TatI w'GTAC_w

Cuts at:   2418    5187    2418 
   Size:       2769    2903
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    448     480     778     810    5189    5221     448 
   Size:         32     298      32    4379      32     899

  Fragments arranged by size: 

                4379     899     298      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   3469    3469 
   Size:       5672
 
XbaI T'CTAG_A

Cuts at:   2703    2703 
   Size:       5672
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3167    3167 
   Size:       5672
 
XhoI C'TCGA_G

Cuts at:   2368    2368 
   Size:       5672
 
XmaI C'CCGG_G

Cuts at:   2326    2692    2326 
   Size:        366    5306
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AleI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI
    BstYI     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NruI     NspI     PciI    PflMI     PfoI     PmeI     PpiI
    PpuMI   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI

 Enzymes that do not cut: 

    AatII    AflII     AhdI     AlfI     AloI     AscI    AvrII     BaeI
    BbvCI    BfrBI     BmtI   Bpu10I    BsiWI     BsmI    BsrGI   BssHII
   BstEII  BstZ17I   Bsu36I     ClaI    CspCI    EcoRV     FseI    FspAI
     HpaI     MfeI     NdeI     NheI     NotI     NsiI     PacI     PasI
     PmlI    PshAI     PsiI     PsrI    SacII    SanDI    SnaBI     SrfI
     SwaI     XmnI     ZraI
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