################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BanII BbrPI BbsI BbvCI BciVI BclI BfrI BglII BlnI BpuAI BsaBI BsaI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI BspDI BspHI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HinfI HpaI KpnI Ksp632I KspI MamI MfeI MluI MluNI MscI MseI MslI MunI NcoI NdeI NheI NlaIII NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccII 12 CGCG CG^CG AccIII 548 TCCGGA T^CCGGA AcyI 16 GGCGCC G[GA]^CG[CT]C AfaI 234 GTAC GT^AC BamHI 386 GGATCC G^GATCC BbeI 16 GGCGCC GGCGC^C BcgI 173 GCACCGTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 44 CCCGG CC^[GC]GG BglI 307 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 493 TACGTG [CT]AC^GT[GA] BsaHI 16 GGCGCC G[GA]^CG[CT]C BseAI 548 TCCGGA T^CCGGA BsgI 190 GTGCAG GTGCAG(16/14) Bsp1407I 233 TGTACA T^GTACA BspEI 548 TCCGGA T^CCGGA BsrFI 320 GCCGGC [GA]^CCGG[CT] BsrGI 233 TGTACA T^GTACA BstBI 181 TTCGAA TT^CGAA BstF5I 546 CATCC GGATG(2/0) BstUI 12 CGCG CG^CG Cfr10I 320 GCCGGC [GA]^CCGG[CT] DraII 603 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoNI 90 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 603 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoT14I 20 CCTTGG C^C[AT][AT]GG FokI 546 CATCC GGATG(9/13) Hin1I 16 GGCGCC G[GA]^CG[CT]C KasI 16 GGCGCC G^GCGCC MaeII 494 ACGT A^CGT MboII 417 GAAGA GAAGA(8/7) MroI 548 TCCGGA T^CCGGA MvnI 12 CGCG CG^CG NaeI 320 GCCGGC GCC^GGC NarI 16 GGCGCC GG^CGCC NciI 44 CCCGG CC^[GC]GG NgoMIV 320 GCCGGC G^CCGGC NspV 181 TTCGAA TT^CGAA PpuMI 603 AGGTCCC [GA]G^G[AT]CC[CT] PvuII 67 CAGCTG CAG^CTG RsaI 234 GTAC GT^AC SfoI 16 GGCGCC GGC^GCC SfuI 181 TTCGAA TT^CGAA SspBI 233 TGTACA T^GTACA StuI 314 AGGCCT AGG^CCT StyI 20 CCTTGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### Aor51HI 5 AGCGCT AGC^GCT 122 AGCGCT BfaI 367 CTAG C^TAG 598 CTAG BlpI 141 GCTGAGC GC^T[ACGT]AGC 352 GCTCAGC Bpu1102I 141 GCTGAGC GC^T[ACGT]AGC 352 GCTCAGC BseRI 25 GAGGAG GAGGAG(10/8) 394 GAGGAG BsmFI 605 GTCCC GGGAC(10/14) 652 GTCCC BspMI 332 ACCTGC ACCTGC(4/8) 533 ACCTGC BstAPI 264 GCATCGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 279 GCAGGCGCTGC BstYI 206 AGATCC [GA]^GATC[CT] 386 GGATCC CelII 141 GCTGAGC GC^T[ACGT]AGC 352 GCTCAGC Eco47III 5 AGCGCT AGC^GCT 122 AGCGCT HphI 93 GGTGA GGTGA(8/7) 218 TCACC MaeI 367 CTAG C^TAG 598 CTAG MflI 206 AGATCC [GA]^GATC[CT] 386 GGATCC MspA1I 67 CAGCTG C[AC]G^C[GT]G 400 CAGCGG PstI 523 CTGCAG CTGCA^G 556 CTGCAG SfaNI 519 GATGC GCATC(5/9) 264 GCATC Tsp45I 496 GTGAC ^GT[GC]AC 632 GTGAC TspRI 464 CACTG CA[GC]TG[ACGT][ACGT]^ 628 CAGTG XhoII 206 AGATCC [GA]^GATC[CT] 386 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AluI AlwI AlwNI AspHI AvaII BanI BbvI BmyI BpmI BsaJI BsaWI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI DpnII EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MaeIII MboI MnlI MspI MvaI MwoI NdeII NlaIV Sau3AI Sau96I ScrFI SfcI TaqI TseI TthHB8I