Restriction map for pF1KE0524
##################################
# NO CUT ENZYMES (  136 enzymes) #
##################################

Acc65I      AccI        AclI        AcsI        AflII       
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AviII       AvrII       BamHI       BbeI        BbrPI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BsaI        BsiWI       BsmAI       BsmBI       BsmI        
Bsp1407I    BspDI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrGI       BsrI        BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstYI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DpnII       DraI        DrdI        Eam1105I    Eco47III    
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HindIII     HpaI        
KasI        KpnI        MamI        MboI        MfeI        
MflI        MluI        MseI        MunI        NarI        
NciI        NcoI        NdeI        NdeII       NheI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SalI        Sau3AI      ScaI        
SexAI       SfaNI       SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SspBI       
SspI        StuI        SwaI        TaqI        Tru9I       
Tsp509I     TspRI       TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AatII         559  GACGTC               GACGT^C
AccIII        146  TCCGGA               T^CCGGA
AfaI          387  GTAC                 GT^AC
AflIII        389  ACGTGT               A^C[GA][CT]GT
ApaI          193  GGGCCC               GGGCC^C
AspI          286  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          410  CTCGGG               C^[CT]CG[GA]G
AvaII         513  GGACC                G^G[AT]CC
BalI          374  TGGCCA               TGG^CCA
BbvCI         105  CCTCAGC              CCTCAGC(-5/-2)
BfaI           16  CTAG                 C^TAG
BsaAI         388  TACGTG               [CT]AC^GT[GA]
BsaWI         146  TCCGGA               [AT]^CCGG[AT]
BseAI         146  TCCGGA               T^CCGGA
BseRI         103  CTCCTC               GAGGAG(10/8)
BsgI           91  GTGCAG               GTGCAG(16/14)
BsoBI         410  CTCGGG               C^[CT]CG[GA]G
BspEI         146  TCCGGA               T^CCGGA
BsrFI          39  GCCGGC               [GA]^CCGG[CT]
BssSI         508  CTCGTG               CACGAG(-5/-1)
BstF5I        504  CATCC                GGATG(2/0)
BstXI         503  CCATCCTCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          469  GCGC                 GCG^C
Cfr10I         39  GCCGGC               [GA]^CCGG[CT]
DdeI          106  CTCAG                C^T[ACGT]AG
DraIII        136  CACCTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI         357  CCTGGGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       463  CCTTGG               C^C[AT][AT]GG
FokI          504  CATCC                GGATG(9/13)
HaeII         468  GGCGCT               [GA]GCGC^[CT]
HhaI          469  GCGC                 GCG^C
HinP1I        469  GCGC                 G^CGC
HincII        421  GTCAAC               GT[CT]^[GA]AC
HindII        421  GTCAAC               GT[CT]^[GA]AC
MaeI           16  CTAG                 C^TAG
MluNI         374  TGGCCA               TGG^CCA
MroI          146  TCCGGA               T^CCGGA
MscI          374  TGGCCA               TGG^CCA
NaeI           39  GCCGGC               GCC^GGC
NgoMIV         39  GCCGGC               G^CCGGC
NotI          272  GCGGCCGC             GC^GGCCGC
PflFI         286  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          335  GACTC                GAGTC(4/5)
RsaI          387  GTAC                 GT^AC
SapI          225  GCTCTTC              GCTCTTC(1/4)
SbfI           30  CCTGCAGG             CCTGCA^GG
SfiI          366  GGCCCTCATGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI          248  GCATGC               GCATG^C
Sse8387I       30  CCTGCAGG             CCTGCA^GG
StyI          463  CCTTGG               C^C[AT][AT]GG
TfiI          203  GATTC                G^A[AT]TC
Tth111I       286  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcyI          313  GGCGTC               G[GA]^CG[CT]C
              559  GACGTC              
AspHI         100  GAGCTC               G[AT]GC[AT]^C
              700  GAGCTC              
BbsI          292  GTCTTC               GAAGAC(2/6)
              562  GTCTTC              
BglI          367  GCCCTCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              674  GCCAGGGCGGC         
BpuAI         292  GTCTTC               GAAGAC(2/6)
              562  GTCTTC              
BsaHI         313  GGCGTC               G[GA]^CG[CT]C
              559  GACGTC              
BsiHKAI       100  GAGCTC               G[AT]GC[AT]^C
              700  GAGCTC              
BspMI         515  ACCTGC               ACCTGC(4/8)
               33  GCAGGT              
DsaI           86  CCGCGG               C^C[GA][CT]GG
              270  CCGCGG              
FauI          132  CCCGC                CCCGC(4/6)
               51  GCGGG               
HapII          40  CCGG                 C^CGG
              147  CCGG                
HgaI           48  GACGC                GACGC(5/10)
              314  GCGTC               
Hin1I         313  GGCGTC               G[GA]^CG[CT]C
              559  GACGTC              
HinfI         203  GATTC                G^A[ACGT]TC
              335  GACTC               
HpaII          40  CCGG                 C^CGG
              147  CCGG                
KspI           86  CCGCGG               CCGC^GG
              270  CCGCGG              
MaeII         389  ACGT                 A^CGT
              560  ACGT                
MaeIII         46  GTGAC                ^GT[ACGT]AC
              172  GTCAC               
MspI           40  CCGG                 C^CGG
              147  CCGG                
NspI          221  ACATGC               [GA]CATG^[CT]
              248  GCATGC              
PstI           31  CTGCAG               CTGCA^G
              595  CTGCAG              
PvuII          94  CAGCTG               CAG^CTG
              638  CAGCTG              
SacI          100  GAGCTC               GAGCT^C
              700  GAGCTC              
SacII          86  CCGCGG               CCGC^GG
              270  CCGCGG              
SfcI           31  CTGCAG               C^T[GA][CT]AG
              595  CTGCAG              
Tsp45I         46  GTGAC                ^GT[GC]AC
              172  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AccII       AciI        AluI        BanI        BanII       
BbvI        BmyI        BpmI        BsaJI       BsiEI       
BsiYI       BslI        BsmFI       Bsp1286I    BssKI       
BstNI       BstUI       Cac8I       Cfr13I      DraII       
EaeI        EagI        EarI        EclXI       Eco52I      
EcoO109I    EcoRII      Fnu4HI      HaeIII      HphI        
ItaI        Ksp632I     MboII       MnlI        MslI        
MspA1I      MvaI        MvnI        MwoI        NlaIII      
NlaIV       Sau96I      ScrFI       TseI        
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