Restriction map for pF1KE0365
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
AlwI        Aor51HI     ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AvaI        AviII       
AvrII       BamHI       BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BpmI        BsaAI       BsaBI       BsaI        
BseAI       BsiWI       BsmBI       BsmI        BsoBI       
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrDI       BssHII      BssSI       Bst1107I    BstBI       
BstYI       BstZ17I     ClaI        CpoI        DraI        
DraIII      Eam1105I    Eco47III    EcoNI       EcoRI       
EcoRV       FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        MamI        
MflI        MluI        MroI        NdeI        NheI        
NotI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspI        StuI        SwaI        TfiI        
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

Alw44I        787  GTGCAC               G^TGCAC
ApaLI         787  GTGCAC               G^TGCAC
Asp700I       171  GAACTGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI          821  GGCGCC               GGCGC^C
BbsI          367  GTCTTC               GAAGAC(2/6)
BcnI          161  CCCGG                CC^[GC]GG
BfaI          836  CTAG                 C^TAG
BpuAI         367  GTCTTC               GAAGAC(2/6)
BseRI         211  GAGGAG               GAGGAG(10/8)
BsgI         1203  CTGCAC               GTGCAG(16/14)
BsiEI         132  CGGCCG               CG[GA][CT]^CG
BsmAI         432  GAGAC                GTCTC(1/5)
BsmFI        1115  GGGAC                GGGAC(10/14)
Bsp1407I     1022  TGTACA               T^GTACA
BsrBI         817  GAGCGG               CCGCTC(-3/-3)
BsrGI        1022  TGTACA               T^GTACA
BstAPI       1065  GCAGGAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         878  CCAAGACTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI          132  CGGCCG               C^GGCCG
EarI         1353  GAAGAG               CTCTTC(1/4)
EclXI         132  CGGCCG               C^GGCCG
Eco52I        132  CGGCCG               C^GGCCG
HinfI        1235  GAGTC                G^A[ACGT]TC
KasI          821  GGCGCC               G^GCGCC
Ksp632I      1353  GAAGAG               CTCTTC(1/4)
KspI         1197  CCGCGG               CCGC^GG
MaeI          836  CTAG                 C^TAG
MaeII        1214  ACGT                 A^CGT
MfeI          636  CAATTG               C^AATTG
MseI          412  TTAA                 T^TAA
MunI          636  CAATTG               C^AATTG
NaeI         1061  GCCGGC               GCC^GGC
NarI          821  GGCGCC               GG^CGCC
NciI          161  CCCGG                CC^[GC]GG
NcoI          545  CCATGG               C^CATGG
NgoMIV       1061  GCCGGC               G^CCGGC
NspI          522  GCATGC               [GA]CATG^[CT]
PleI         1235  GAGTC                GAGTC(4/5)
PpuMI         224  GGGTCCT              [GA]G^G[AT]CC[CT]
PvuII         235  CAGCTG               CAG^CTG
SacI          679  GAGCTC               GAGCT^C
SacII        1197  CCGCGG               CCGC^GG
SfoI          821  GGCGCC               GGC^GCC
SmlI          219  CTTGAG               C^T[CT][GA]AG
SphI          522  GCATGC               GCATG^C
SspBI        1022  TGTACA               T^GTACA
TaqI         1334  TCGA                 T^CGA
Tru9I         412  TTAA                 T^TAA
Tsp509I       637  AATT                 ^AATT
TthHB8I      1334  TCGA                 T^CGA
XmnI          171  GAACTGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AocI           30  CCTCAGG              CC^T[ACGT]AGG
             1309  CCTGAGG             
ApaI          276  GGGCCC               GGGCC^C
             1390  GGGCCC              
AspI          313  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              361  GACATAGTC           
BanI          821  GGCGCC               G^G[CT][GA]CC
             1037  GGCACC              
BlpI            9  GCTGAGC              GC^T[ACGT]AGC
              909  GCTGAGC             
Bpu1102I        9  GCTGAGC              GC^T[ACGT]AGC
              909  GCTGAGC             
BsaWI         143  TCCGGT               [AT]^CCGG[AT]
              719  TCCGGT              
BsrFI          36  GCCGGT               [GA]^CCGG[CT]
             1061  GCCGGC              
BstEII        184  GGTCACC              G^GT[ACGT]ACC
             1358  GGTCACC             
BstPI         184  GGTCACC              G^GT[ACGT]ACC
             1358  GGTCACC             
Bsu36I         30  CCTCAGG              CC^T[ACGT]AGG
             1309  CCTGAGG             
CelII           9  GCTGAGC              GC^T[ACGT]AGC
              909  GCTGAGC             
Cfr10I         36  GCCGGT               [GA]^CCGG[CT]
             1061  GCCGGC              
DpnII         323  GATC                 ^GATC
              672  GATC                
Eco81I         30  CCTCAGG              CC^T[ACGT]AGG
             1309  CCTGAGG             
EcoO65I       184  GGTCACC              G^GT[ACGT]ACC
             1358  GGTCACC             
EcoT14I       545  CCATGG               C^C[AT][AT]GG
              866  CCAAGG              
EcoT22I       520  ATGCAT               ATGCA^T
              615  ATGCAT              
HgaI          453  GACGC                GACGC(5/10)
             1500  GACGC               
MboI          323  GATC                 ^GATC
              672  GATC                
MslI           47  CATCCCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              969  CACTTTCATG          
MspA1I        235  CAGCTG               C[AC]G^C[GT]G
             1197  CCGCGG              
NdeII         323  GATC                 ^GATC
              672  GATC                
NruI         1031  TCGCGA               TCG^CGA
             1418  TCGCGA              
NsiI          520  ATGCAT               ATGCA^T
              615  ATGCAT              
PflFI         313  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              361  GACATAGTC           
PflMI         234  CCAGCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1209  CCAGGACGTGG         
PstI          607  CTGCAG               CTGCA^G
              777  CTGCAG              
Sau3AI        323  GATC                 ^GATC
              672  GATC                
SfcI          607  CTGCAG               C^T[GA][CT]AG
              777  CTGCAG              
StyI          545  CCATGG               C^C[AT][AT]GG
              866  CCAAGG              
Tth111I       313  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              361  GACATAGTC           
Van91I        234  CCAGCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1209  CCAGGACGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       AspHI       AvaII       BalI        BanII       
BbvI        BmyI        BsaHI       BsaJI       BsiHKAI     
BsiYI       BslI        Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        DraII       DrdI        DsaI        
EaeI        EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
Hin1I       HinP1I      HpaII       HphI        ItaI        
MaeIII      MboII       MluNI       MnlI        MscI        
MspI        MvaI        MvnI        MwoI        NlaIII      
NlaIV       RsaI        Sau96I      ScrFI       SfaNI       
TseI        Tsp45I      TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com