Restriction map for pF1KE0365
 (Circular) (Six-base) MAPSORT of: pF1KE0365.seq  Check: 692  from: 1  to: 4673



pF1KE0365  4673 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AccI GT'mk_AC

Cuts at:   1711    1711 
   Size:       4673
 
Acc65I G'GTAC_C

Cuts at:   1689    1689 
   Size:       4673
 
AclI AA'CG_TT

Cuts at:   4513    4513 
   Size:       4673
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    511     607     714    1213    1282    2498    2930    3677    3945 
   Size:         96     107     499      69    1216     432     747     268

Cuts at:   3945     511 
   Size:       1239

  Fragments arranged by size: 

                1239    1216     747     499     432     268     107      96
                  69
 
AflIII A'CryG_T

Cuts at:   2025    3129    4106    2025 
   Size:       1104     977    2592

  Fragments arranged by size: 

                2592    1104     977
 
AgeI A'CCGG_T

Cuts at:   3061    3061 
   Size:       4673
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    910     910 
   Size:       4673
 
AlwNI CAG_nnn'CTG

Cuts at:    668     884    1072    1417    1508    1978    3545     668 
   Size:        216     188     345      91     470    1567    1796

  Fragments arranged by size: 

                1796    1567     470     345     216     188      91
 
ApaI G_GGCC'C

Cuts at:    388    1502     388 
   Size:       1114    3559
 
ApaLI G'TGCA_C

Cuts at:    895    3443     895 
   Size:       2548    2125
 
ApoI r'AATT_y

Cuts at:   1677    4026    4282    1677 
   Size:       2349     256    2068

  Fragments arranged by size: 

                2349    2068     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4673
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4673
 
AvaI C'yCGr_G

Cuts at:   1693    1693 
   Size:       4673
 
BamHI G'GATC_C

Cuts at:   1698    1698 
   Size:       4673
 
BanI G'GyrC_C

Cuts at:    929    1145    1689    2103    2351    2386     929 
   Size:        216     544     414     248      35    3216

  Fragments arranged by size: 

                3216     544     414     248     216      35
 
BanII G_rGCy'C

Cuts at:    388     515     791    1502    1687    2717     388 
   Size:        127     276     711     185    1030    2344

  Fragments arranged by size: 

                2344    1030     711     276     185     127
 
BbeI G_GCGC'C

Cuts at:    933    2107    2355     933 
   Size:       1174     248    3251

  Fragments arranged by size: 

                3251    1174     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    468     468 
   Size:       4673
 
BciVI GTATCCnnnnn_n'

Cuts at:   2571    3332    4673    2571 
   Size:        761    1341    2571

  Fragments arranged by size: 

                2571    1341     761
 
BclI T'GATC_A

Cuts at:   2193    2193 
   Size:       4673
 
BfrBI ATG'CAT

Cuts at:    630     725     630 
   Size:         95    4578
 
BglII A'GATC_T

Cuts at:   2188    4225    2188 
   Size:       2037    2636
 
BlpI GC'TnA_GC

Cuts at:    118    1018    1785     118 
   Size:        900     767    3006

  Fragments arranged by size: 

                3006     900     767
 
Bme1580I G_kGCm'C

Cuts at:    388     798     899    1502    2298    2391    3447     388 
   Size:        410     101     603     796      93    1056    1614

  Fragments arranged by size: 

                1614    1056     796     603     410     101      93
 
BmgBI CAC'GTC

Cuts at:   1323    1323 
   Size:       4673
 
BmrI ACTGGGnnnn_n'

Cuts at:    755    1230    1885    1966    2301    4445     755 
   Size:        475     655      81     335    2144     983

  Fragments arranged by size: 

                2144     983     655     475     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3040    3040 
   Size:       4673
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    348    1849    2728    3220    3518     348 
   Size:       1501     879     492     298    1503

  Fragments arranged by size: 

                1503    1501     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4377    4377 
   Size:       4673
 
BsaAI yAC'GTr

Cuts at:   2026    2026 
   Size:       4673
 
BsaBI GATnn'nnATC

Cuts at:      6    2187    4224       6 
   Size:       2181    2037     455

  Fragments arranged by size: 

                2181    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    562     930    1609    2104    2352    3054    4550     562 
   Size:        368     679     495     248     702    1496     685

  Fragments arranged by size: 

                1496     702     685     679     495     368     248
 
BsaWI w'CCGG_w

Cuts at:    251     827    1862    2383    3061    3335    3482    3893     251 
   Size:        576    1035     521     678     274     147     411    1031

  Fragments arranged by size: 

                1035    1031     678     576     521     411     274     147
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    334     334 
   Size:       4673
 
BseYI C'CCAG_C

Cuts at:    132     341     501    2098    2856    3433    3929     132 
   Size:        209     160    1597     758     577     496     876

  Fragments arranged by size: 

                1597     876     758     577     496     209     160
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1296    1296 
   Size:       4673
 
BsiEI CG_ry'CG

Cuts at:    104     243    2215    2261    3469     104 
   Size:        139    1972      46    1208    1308

  Fragments arranged by size: 

                1972    1308    1208     139      46
 
BsiHKAI G_wGCw'C

Cuts at:    689     791     899    1013    1331    1687    2465    2655    3447 
   Size:        102     108     114     318     356     778     190     792

Cuts at:   3447     689 
   Size:       1915

  Fragments arranged by size: 

                1915     792     778     356     318     190     114     108
                 102
 
Bsp1286I G_dGCh'C

Cuts at:    388     515     689     791     798     899    1013    1331    1502 
   Size:        127     174     102       7     101     114     318     171

Cuts at:   1502    1687    2298    2391    2465    2655    2717    3447     388 
   Size:        185     611      93      74     190      62     730    1614

  Fragments arranged by size: 

                1614     730     611     318     190     185     174     171
                 127     114     102     101      93      74      62       7
            
 
BspEI T'CCGG_A

Cuts at:   3893    3893 
   Size:       4673
 
BspHI T'CATG_A

Cuts at:   3849    3849 
   Size:       4673
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1723    1937    2239    2620    1723 
   Size:        214     302     381    3776

  Fragments arranged by size: 

                3776     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     927    2965    3019    4503    4668      50 
   Size:        877    2038      54    1484     165      55

  Fragments arranged by size: 

                2038    1484     877     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2585    2585 
   Size:       4673
 
BsrFI r'CCGG_y

Cuts at:    144    1169    2671    2852    3061     144 
   Size:       1025    1502     181     209    1756

  Fragments arranged by size: 

                1756    1502    1025     209     181
 
BsrGI T'GTAC_A

Cuts at:   1130    1130 
   Size:       4673
 
BssSI C'ACGA_G

Cuts at:   2944    3302    2944 
   Size:        358    4315
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1179    2050    1179 
   Size:        871    3802
 
BstBI TT'CG_AA

Cuts at:   3034    3034 
   Size:       4673
 
BstEII G'GTnAC_C

Cuts at:    292    1466     292 
   Size:       1174    3499
 
BstXI CCAn_nnnn'nTGG

Cuts at:    993     993 
   Size:       4673
 
BstYI r'GATC_y

Cuts at:      1    1698    2165    2188    2523    2769    3781    3867    3879 
   Size:       1697     467      23     335     246    1012      86      12

Cuts at:   3879    4225       1 
   Size:        346     449

  Fragments arranged by size: 

                1697    1012     467     449     346     335     246      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:    139    1418     139 
   Size:       1279    3394
 
BtgI C'CryG_G

Cuts at:    160     653    1103    1305     160 
   Size:        493     450     202    3528

  Fragments arranged by size: 

                3528     493     450     202
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1543    2623    2804    2967    1543 
   Size:       1080     181     163    3249

  Fragments arranged by size: 

                3249    1080     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1916    1916 
   Size:       4673
 
DraI TTT'AAA

Cuts at:   1672    1672 
   Size:       4673
 
DrdI GACnn_nn'nnGTC

Cuts at:    548    1342    1420    2379    3237     548 
   Size:        794      78     959     858    1984

  Fragments arranged by size: 

                1984     959     858     794      78
 
EaeI y'GGCC_r

Cuts at:    240     350     650    1106    1408    2258    2432    2823    2850 
   Size:        110     300     456     302     850     174     391      27

Cuts at:   2850    4195     240 
   Size:       1345     718

  Fragments arranged by size: 

                1345     850     718     456     391     302     300     174
                 110      27
 
EagI C'GGCC_G

Cuts at:    240    2258     240 
   Size:       2018    2655
 
EarI CTCTTCn'nnn_

Cuts at:   1456    2696    2906    1456 
   Size:       1240     210    3223

  Fragments arranged by size: 

                3223    1240     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2881    3191    3337    4483    2881 
   Size:        310     146    1146    3071

  Fragments arranged by size: 

                3071    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    789    1685     789 
   Size:        896    3777
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    511     607     714    1213    1282    2498    2930    3677    3945 
   Size:         96     107     499      69    1216     432     747     268

Cuts at:   3945     511 
   Size:       1239

  Fragments arranged by size: 

                1239    1216     747     499     432     268     107      96
                  69
 
EcoO109I rG'GnC_Cy

Cuts at:    185     333     385     881    1498    1812     185 
   Size:        148      52     496     617     314    3046

  Fragments arranged by size: 

                3046     617     496     314     148      52
 
EcoRI G'AATT_C

Cuts at:   1677    1677 
   Size:       4673
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2692    2692 
   Size:       4673
 
FspI TGC'GCA

Cuts at:   2454    2454 
   Size:       4673
 
HaeII r_GCGC'y

Cuts at:    873     933    1558    2107    2355    3377    4356     873 
   Size:         60     625     549     248    1022     979    1190

  Fragments arranged by size: 

                1190    1022     979     625     549     248      60
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    422     454    2515    2545    2547    2577     422 
   Size:         32    2061      30       2      30    2518

  Fragments arranged by size: 

                2518    2061      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1712    1712 
   Size:       4673
 
KasI G'GCGC_C

Cuts at:    929    2103    2351     929 
   Size:       1174     248    3251

  Fragments arranged by size: 

                3251    1174     248
 
KpnI G_GTAC'C

Cuts at:   1693    1693 
   Size:       4673
 
MfeI C'AATT_G

Cuts at:    744     744 
   Size:       4673
 
MluI A'CGCG_T

Cuts at:   4106    4106 
   Size:       4673
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3344    3528    3344 
   Size:        184    4489
 
MscI TGG'CCA

Cuts at:    352     652    1410    2434     352 
   Size:        300     758    1024    2591

  Fragments arranged by size: 

                2591    1024     758     300
 
MslI CAynn'nnrTG

Cuts at:    159    1081    2662    2789     159 
   Size:        922    1581     127    2043

  Fragments arranged by size: 

                2043    1581     922     127
 
MspA1I CmG'CkG

Cuts at:    345    1307    1784    2098    2458    3471    3716     345 
   Size:        962     477     314     360    1013     245    1302

  Fragments arranged by size: 

                1302    1013     962     477     360     314     245
 
NaeI GCC'GGC

Cuts at:   1171    2854    1171 
   Size:       1683    2990
 
NarI GG'CG_CC

Cuts at:    930    2104    2352     930 
   Size:       1174     248    3251

  Fragments arranged by size: 

                3251    1174     248
 
NcoI C'CATG_G

Cuts at:    653     653 
   Size:       4673
 
NgoMIV G'CCGG_C

Cuts at:   1169    2852    1169 
   Size:       1683    2990
 
NruI TCG'CGA

Cuts at:   1141    1528    1141 
   Size:        387    4286
 
NsiI A_TGCA'T

Cuts at:    632     727     632 
   Size:         95    4578
 
NspI r_CATG'y

Cuts at:    634    1726    2226    3133     634 
   Size:       1092     500     907    2174

  Fragments arranged by size: 

                2174    1092     907     500
 
PasI CC'CwG_GG

Cuts at:    363    1662     363 
   Size:       1299    3374
 
PciI A'CATG_T

Cuts at:   3129    3129 
   Size:       4673
 
PflMI CCAn_nnn'nTGG

Cuts at:    348    1323     348 
   Size:        975    3698
 
PfoI T'CCnGG_A

Cuts at:    920     920 
   Size:       4673
 
PmeI GTTT'AAAC

Cuts at:   1672    1672 
   Size:       4673
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3812    3844    3812 
   Size:         32    4641
 
PpuMI rG'GwC_Cy

Cuts at:    333     333 
   Size:       4673
 
PspOMI G'GGCC_C

Cuts at:    384    1498     384 
   Size:       1114    3559
 
PstI C_TGCA'G

Cuts at:    719     889    1720    2405     719 
   Size:        170     831     685    2987

  Fragments arranged by size: 

                2987     831     685     170
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4673
 
PvuII CAG'CTG

Cuts at:    345    2098    2458     345 
   Size:       1753     360    2560

  Fragments arranged by size: 

                2560    1753     360
 
RsrII CG'GwC_CG

Cuts at:   2868    2868 
   Size:       4673
 
SacI G_AGCT'C

Cuts at:    791    1687     791 
   Size:        896    3777
 
SacII CC_GC'GG

Cuts at:   1308    1308 
   Size:       4673
 
SalI G'TCGA_C

Cuts at:   1710    1710 
   Size:       4673
 
SapI GCTCTTCn'nnn_

Cuts at:   2696    2906    2696 
   Size:        210    4463
 
SbfI CC_TGCA'GG

Cuts at:   1720    1720 
   Size:       4673
 
ScaI AGT'ACT

Cuts at:   4190    4190 
   Size:       4673
 
SfcI C'TryA_G

Cuts at:     33     715     885    1716    2401    3394    3585      33 
   Size:        682     170     831     685     993     191    1121

  Fragments arranged by size: 

                1121     993     831     685     682     191     170
 
SfoI GGC'GCC

Cuts at:    931    2105    2353     931 
   Size:       1174     248    3251

  Fragments arranged by size: 

                3251    1174     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4673
 
SmaI CCC'GGG

Cuts at:   1695    1695 
   Size:       4673
 
SmlI C'TyrA_G

Cuts at:    327    1828    2743    3235    3497     327 
   Size:       1501     915     492     262    1503

  Fragments arranged by size: 

                1503    1501     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4673
 
SphI G_CATG'C

Cuts at:    634    1726    2226     634 
   Size:       1092     500    3081

  Fragments arranged by size: 

                3081    1092     500
 
StyI C'CwwG_G

Cuts at:    653     974    1807    2161     653 
   Size:        321     833     354    3165

  Fragments arranged by size: 

                3165     833     354     321
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1163    1330    2256    2389    2848    3057    1163 
   Size:        167     926     133     459     209    2779

  Fragments arranged by size: 

                2779     926     459     209     167     133
 
TatI w'GTAC_w

Cuts at:    845    1130    4188     845 
   Size:        285    3058    1330

  Fragments arranged by size: 

                3058    1330     285
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4190    4222    4190 
   Size:         32    4641
 
Tth111I GACn'n_nGTC

Cuts at:    424     472    2470     424 
   Size:         48    1998    2627

  Fragments arranged by size: 

                2627    1998      48
 
XbaI T'CTAG_A

Cuts at:   1704    1704 
   Size:       4673
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2168    2168 
   Size:       4673
 
XmaI C'CCGG_G

Cuts at:   1693    1693 
   Size:       4673
 
XmnI GAAnn'nnTTC

Cuts at:    283     283 
   Size:       4673
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NruI     NsiI     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI   PspOMI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AloI     AscI
    AvrII     BaeI    BbvCI     BcgI     BglI     BmtI     BpmI   Bpu10I
    BsaXI    BsiWI     BsmI    BsmBI   BssHII  BstZ17I     ClaI    CspCI
   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     NdeI
     NheI     NotI     PacI     PmlI    PshAI     PsiI    PspXI     PsrI
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI
     SwaI     XhoI     ZraI
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