Restriction map for pF1KE0365
 (Linear) (Six-base) MAPSORT of: KIEE0365.seq  Check: 6748  from: 1  to: 1560



KIEE0365.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     403     499     606    1105    1174    1560 
   Size:        403      96     107     499      69     386

  Fragments arranged by size: 

                 499     403     386     107      96      69
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     802    1560 
   Size:        802     758
 
AlwNI CAG_nnn'CTG

Cuts at:      0     560     776     964    1309    1400    1560 
   Size:        560     216     188     345      91     160

  Fragments arranged by size: 

                 560     345     216     188     160      91
 
ApaI G_GGCC'C

Cuts at:      0     280    1394    1560 
   Size:        280    1114     166

  Fragments arranged by size: 

                1114     280     166
 
ApaLI G'TGCA_C

Cuts at:      0     787    1560 
   Size:        787     773
 
BanI G'GyrC_C

Cuts at:      0     821    1037    1560 
   Size:        821     216     523

  Fragments arranged by size: 

                 821     523     216
 
BanII G_rGCy'C

Cuts at:      0     280     407     683    1394    1560 
   Size:        280     127     276     711     166

  Fragments arranged by size: 

                 711     280     276     166     127
 
BbeI G_GCGC'C

Cuts at:      0     825    1560 
   Size:        825     735
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     360    1560 
   Size:        360    1200
 
BfrBI ATG'CAT

Cuts at:      0     522     617    1560 
   Size:        522      95     943

  Fragments arranged by size: 

                 943     522      95
 
BlpI GC'TnA_GC

Cuts at:      0      10     910    1560 
   Size:         10     900     650

  Fragments arranged by size: 

                 900     650      10
 
Bme1580I G_kGCm'C

Cuts at:      0     280     690     791    1394    1560 
   Size:        280     410     101     603     166

  Fragments arranged by size: 

                 603     410     280     166     101
 
BmgBI CAC'GTC

Cuts at:      0    1215    1560 
   Size:       1215     345
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     647    1122    1560 
   Size:        647     475     438

  Fragments arranged by size: 

                 647     475     438
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     240    1560 
   Size:        240    1320
 
BsaHI Gr'CG_yC

Cuts at:      0     454     822    1501    1560 
   Size:        454     368     679      59

  Fragments arranged by size: 

                 679     454     368      59
 
BsaWI w'CCGG_w

Cuts at:      0     143     719    1560 
   Size:        143     576     841

  Fragments arranged by size: 

                 841     576     143
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     226    1560 
   Size:        226    1334
 
BseYI C'CCAG_C

Cuts at:      0      24     233     393    1560 
   Size:         24     209     160    1167

  Fragments arranged by size: 

                1167     209     160      24
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1188    1560 
   Size:       1188     372
 
BsiEI CG_ry'CG

Cuts at:      0     135    1560 
   Size:        135    1425
 
BsiHKAI G_wGCw'C

Cuts at:      0     581     683     791     905    1223    1560 
   Size:        581     102     108     114     318     337

  Fragments arranged by size: 

                 581     337     318     114     108     102
 
Bsp1286I G_dGCh'C

Cuts at:      0     280     407     581     683     690     791     905    1223 
   Size:        280     127     174     102       7     101     114     318

Cuts at:   1223    1394    1560 
   Size:        171     166

  Fragments arranged by size: 

                 318     280     174     171     166     127     114     102
                 101       7
 
BsrBI CCG'CTC

Cuts at:      0     819    1560 
   Size:        819     741
 
BsrFI r'CCGG_y

Cuts at:      0      36    1061    1560 
   Size:         36    1025     499

  Fragments arranged by size: 

                1025     499      36
 
BsrGI T'GTAC_A

Cuts at:      0    1022    1560 
   Size:       1022     538
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1071    1560 
   Size:       1071     489
 
BstEII G'GTnAC_C

Cuts at:      0     184    1358    1560 
   Size:        184    1174     202

  Fragments arranged by size: 

                1174     202     184
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     885    1560 
   Size:        885     675
 
Bsu36I CC'TnA_GG

Cuts at:      0      31    1310    1560 
   Size:         31    1279     250

  Fragments arranged by size: 

                1279     250      31
 
BtgI C'CryG_G

Cuts at:      0      52     545     995    1197    1560 
   Size:         52     493     450     202     363

  Fragments arranged by size: 

                 493     450     363     202      52
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1435    1560 
   Size:       1435     125
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     440    1234    1312    1560 
   Size:        440     794      78     248

  Fragments arranged by size: 

                 794     440     248      78
 
EaeI y'GGCC_r

Cuts at:      0     132     242     542     998    1300    1560 
   Size:        132     110     300     456     302     260

  Fragments arranged by size: 

                 456     302     300     260     132     110
 
EagI C'GGCC_G

Cuts at:      0     132    1560 
   Size:        132    1428
 
EarI CTCTTCn'nnn_

Cuts at:      0    1348    1560 
   Size:       1348     212
 
EcoICRI GAG'CTC

Cuts at:      0     681    1560 
   Size:        681     879
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     403     499     606    1105    1174    1560 
   Size:        403      96     107     499      69     386

  Fragments arranged by size: 

                 499     403     386     107      96      69
 
EcoO109I rG'GnC_Cy

Cuts at:      0      77     225     277     773    1390    1560 
   Size:         77     148      52     496     617     170

  Fragments arranged by size: 

                 617     496     170     148      77      52
 
HaeII r_GCGC'y

Cuts at:      0     765     825    1450    1560 
   Size:        765      60     625     110

  Fragments arranged by size: 

                 765     625     110      60
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     314     346    1560 
   Size:        314      32    1214

  Fragments arranged by size: 

                1214     314      32
 
KasI G'GCGC_C

Cuts at:      0     821    1560 
   Size:        821     739
 
MfeI C'AATT_G

Cuts at:      0     636    1560 
   Size:        636     924
 
MscI TGG'CCA

Cuts at:      0     244     544    1302    1560 
   Size:        244     300     758     258

  Fragments arranged by size: 

                 758     300     258     244
 
MslI CAynn'nnrTG

Cuts at:      0      51     973    1560 
   Size:         51     922     587

  Fragments arranged by size: 

                 922     587      51
 
MspA1I CmG'CkG

Cuts at:      0     237    1199    1560 
   Size:        237     962     361

  Fragments arranged by size: 

                 962     361     237
 
NaeI GCC'GGC

Cuts at:      0    1063    1560 
   Size:       1063     497
 
NarI GG'CG_CC

Cuts at:      0     822    1560 
   Size:        822     738
 
NcoI C'CATG_G

Cuts at:      0     545    1560 
   Size:        545    1015
 
NgoMIV G'CCGG_C

Cuts at:      0    1061    1560 
   Size:       1061     499
 
NruI TCG'CGA

Cuts at:      0    1033    1420    1560 
   Size:       1033     387     140

  Fragments arranged by size: 

                1033     387     140
 
NsiI A_TGCA'T

Cuts at:      0     524     619    1560 
   Size:        524      95     941

  Fragments arranged by size: 

                 941     524      95
 
NspI r_CATG'y

Cuts at:      0     526    1560 
   Size:        526    1034
 
PasI CC'CwG_GG

Cuts at:      0     255    1554    1560 
   Size:        255    1299       6

  Fragments arranged by size: 

                1299     255       6
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     240    1215    1560 
   Size:        240     975     345

  Fragments arranged by size: 

                 975     345     240
 
PfoI T'CCnGG_A

Cuts at:      0     812    1560 
   Size:        812     748
 
PpuMI rG'GwC_Cy

Cuts at:      0     225    1560 
   Size:        225    1335
 
PspOMI G'GGCC_C

Cuts at:      0     276    1390    1560 
   Size:        276    1114     170

  Fragments arranged by size: 

                1114     276     170
 
PstI C_TGCA'G

Cuts at:      0     611     781    1560 
   Size:        611     170     779

  Fragments arranged by size: 

                 779     611     170
 
PvuII CAG'CTG

Cuts at:      0     237    1560 
   Size:        237    1323
 
SacI G_AGCT'C

Cuts at:      0     683    1560 
   Size:        683     877
 
SacII CC_GC'GG

Cuts at:      0    1200    1560 
   Size:       1200     360
 
SfcI C'TryA_G

Cuts at:      0     607     777    1560 
   Size:        607     170     783

  Fragments arranged by size: 

                 783     607     170
 
SfoI GGC'GCC

Cuts at:      0     823    1560 
   Size:        823     737
 
SmlI C'TyrA_G

Cuts at:      0     219    1560 
   Size:        219    1341
 
SphI G_CATG'C

Cuts at:      0     526    1560 
   Size:        526    1034
 
StyI C'CwwG_G

Cuts at:      0     545     866    1560 
   Size:        545     321     694

  Fragments arranged by size: 

                 694     545     321
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1055    1222    1560 
   Size:       1055     167     338

  Fragments arranged by size: 

                1055     338     167
 
TatI w'GTAC_w

Cuts at:      0     737    1022    1560 
   Size:        737     285     538

  Fragments arranged by size: 

                 737     538     285
 
Tth111I GACn'n_nGTC

Cuts at:      0     316     364    1560 
   Size:        316      48    1196

  Fragments arranged by size: 

                1196     316      48
 
XmnI GAAnn'nnTTC

Cuts at:      0     175    1560 
   Size:        175    1385
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI     ApaI    ApaLI     BanI    BanII     BbeI
     BbsI    BfrBI     BlpI Bme1580I    BmgBI     BmrI    BpuEI    BsaHI
    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BsrBI
    BsrFI    BsrGI   BstAPI   BstEII    BstXI   Bsu36I     BtgI    BtgZI
     DrdI     EaeI     EagI     EarI  EcoICRI  Eco57MI EcoO109I    HaeII
    Hin4I     KasI     MfeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NruI     NsiI     NspI     PasI    PflMI     PfoI
    PpuMI   PspOMI     PstI    PvuII     SacI    SacII     SfcI     SfoI
     SmlI     SphI     StyI    TaqII     TatI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI
     BglI    BglII     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaXI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrDI   BssHII    BssSI    BstBI    BstYI  BstZ17I     BtsI     ClaI
    CspCI     DraI   DraIII     EciI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MluI
     MmeI     NdeI     NheI     NotI     PacI     PciI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI     TstI
     XbaI     XcmI     XhoI     XmaI     ZraI
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