Restriction map for pF1KB8184
 (Circular) (Six-base) MAPSORT of: pF1KB8184.seq  Check: 6286  from: 1  to: 4079



pF1KB8184  4079 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    688     775     688 
   Size:         87    3992
 
AccI GT'mk_AC

Cuts at:   1117    1117 
   Size:       4079
 
Acc65I G'GTAC_C

Cuts at:   1095    1095 
   Size:       4079
 
AclI AA'CG_TT

Cuts at:   3919    3919 
   Size:       4079
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    643    1904    2336    3083    3351     643 
   Size:       1261     432     747     268    1371

  Fragments arranged by size: 

                1371    1261     747     432     268
 
AflIII A'CryG_T

Cuts at:   1431    2535    3512    1431 
   Size:       1104     977    1998

  Fragments arranged by size: 

                1998    1104     977
 
AgeI A'CCGG_T

Cuts at:   2467    2467 
   Size:       4079
 
AleI CACnn'nnGTG

Cuts at:    518     518 
   Size:       4079
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    617     617 
   Size:       4079
 
AlwNI CAG_nnn'CTG

Cuts at:    439    1384    2951     439 
   Size:        945    1567    1567

  Fragments arranged by size: 

                1567    1567     945
 
ApaLI G'TGCA_C

Cuts at:   2849    2849 
   Size:       4079
 
ApoI r'AATT_y

Cuts at:    982    1083    3432    3688     982 
   Size:        101    2349     256    1373

  Fragments arranged by size: 

                2349    1373     256     101
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4079
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4079
 
AvaI C'yCGr_G

Cuts at:    274    1099     274 
   Size:        825    3254
 
BamHI G'GATC_C

Cuts at:   1104    1104 
   Size:       4079
 
BanI G'GyrC_C

Cuts at:    700    1095    1509    1757    1792     700 
   Size:        395     414     248      35    2987

  Fragments arranged by size: 

                2987     414     395     248      35
 
BanII G_rGCy'C

Cuts at:    311    1093    2123     311 
   Size:        782    1030    2267

  Fragments arranged by size: 

                2267    1030     782
 
BbeI G_GCGC'C

Cuts at:   1513    1761    1513 
   Size:        248    3831
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    649     683     649 
   Size:         34    4045
 
BciVI GTATCCnnnnn_n'

Cuts at:   1977    2738    4079    1977 
   Size:        761    1341    1977

  Fragments arranged by size: 

                1977    1341     761
 
BclI T'GATC_A

Cuts at:   1599    1599 
   Size:       4079
 
BglI GCCn_nnn'nGGC

Cuts at:    253     427     915     253 
   Size:        174     488    3417

  Fragments arranged by size: 

                3417     488     174
 
BglII A'GATC_T

Cuts at:   1594    3631    1594 
   Size:       2037    2042
 
BlpI GC'TnA_GC

Cuts at:    541    1191     541 
   Size:        650    3429
 
Bme1580I G_kGCm'C

Cuts at:   1704    1797    2853    1704 
   Size:         93    1056    2930

  Fragments arranged by size: 

                2930    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1291    1372    1707    3851    1291 
   Size:         81     335    2144    1519

  Fragments arranged by size: 

                2144    1519     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    873    2446     873 
   Size:       1573    2506
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1048    1048 
   Size:       4079
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1255    2134    2626    2924    1255 
   Size:        879     492     298    2410

  Fragments arranged by size: 

                2410     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3783    3783 
   Size:       4079
 
BsaAI yAC'GTr

Cuts at:   1432    1432 
   Size:       4079
 
BsaBI GATnn'nnATC

Cuts at:      6    1593    3630       6 
   Size:       1587    2037     455

  Fragments arranged by size: 

                2037    1587     455
 
BsaHI Gr'CG_yC

Cuts at:   1510    1758    2460    3956    1510 
   Size:        248     702    1496    1633

  Fragments arranged by size: 

                1633    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1061    1268    1789    2467    2741    2888    3299    1061 
   Size:        207     521     678     274     147     411    1841

  Fragments arranged by size: 

                1841     678     521     411     274     207     147
 
BseYI C'CCAG_C

Cuts at:   1504    2262    2839    3335    1504 
   Size:        758     577     496    2248

  Fragments arranged by size: 

                2248     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    242     655     796     960     242 
   Size:        413     141     164    3361

  Fragments arranged by size: 

                3361     413     164     141
 
BsiEI CG_ry'CG

Cuts at:    104    1621    1667    2875     104 
   Size:       1517      46    1208    1308

  Fragments arranged by size: 

                1517    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1093    1871    2061    2853    1093 
   Size:        778     190     792    2319

  Fragments arranged by size: 

                2319     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    311    1093    1704    1797    1871    2061    2123    2853     311 
   Size:        782     611      93      74     190      62     730    1537

  Fragments arranged by size: 

                1537     782     730     611     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   3299    3299 
   Size:       4079
 
BspHI T'CATG_A

Cuts at:   3255    3255 
   Size:       4079
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    688     775    1129    1343    1645    2026     688 
   Size:         87     354     214     302     381    2741

  Fragments arranged by size: 

                2741     381     354     302     214      87
 
BsrBI CCG'CTC

Cuts at:     50     293     332     342     555    2371    2425    3909    4074 
   Size:        243      39      10     213    1816      54    1484     165

Cuts at:   4074      50 
   Size:         55

  Fragments arranged by size: 

                1816    1484     243     213     165      55      54      39
                  10
 
BsrDI GCAATG_nn'

Cuts at:   1991    1991 
   Size:       4079
 
BsrFI r'CCGG_y

Cuts at:    139     914    2077    2258    2467     139 
   Size:        775    1163     181     209    1751

  Fragments arranged by size: 

                1751    1163     775     209     181
 
BssHII G'CGCG_C

Cuts at:    806     905     806 
   Size:         99    3980
 
BssSI C'ACGA_G

Cuts at:   2350    2708    2350 
   Size:        358    3721
 
BstAPI GCAn_nnn'nTGC

Cuts at:    439     929    1456     439 
   Size:        490     527    3062

  Fragments arranged by size: 

                3062     527     490
 
BstBI TT'CG_AA

Cuts at:   2440    2440 
   Size:       4079
 
BstXI CCAn_nnnn'nTGG

Cuts at:    486     567     486 
   Size:         81    3998
 
BstYI r'GATC_y

Cuts at:      1    1104    1571    1594    1929    2175    3187    3273    3285 
   Size:       1103     467      23     335     246    1012      86      12

Cuts at:   3285    3631       1 
   Size:        346     449

  Fragments arranged by size: 

                1103    1012     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    479     560     479 
   Size:         81    3998
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2029    2210    2373    2029 
   Size:        181     163    3735

  Fragments arranged by size: 

                3735     181     163
 
BtsI GCAGTG_nn'

Cuts at:    186     195     230     375     422     943    1322     186 
   Size:          9      35     145      47     521     379    2943

  Fragments arranged by size: 

                2943     521     379     145      47      35       9
 
DraI TTT'AAA

Cuts at:   1078    1078 
   Size:       4079
 
DrdI GACnn_nn'nnGTC

Cuts at:   1785    2643    1785 
   Size:        858    3221
 
EaeI y'GGCC_r

Cuts at:    452     476     530     717     797     966    1664    1838    2229 
   Size:         24      54     187      80     169     698     174     391

Cuts at:   2229    2256    3601     452 
   Size:         27    1345     930

  Fragments arranged by size: 

                1345     930     698     391     187     174     169      80
                  54      27      24
 
EagI C'GGCC_G

Cuts at:   1664    1664 
   Size:       4079
 
EarI CTCTTCn'nnn_

Cuts at:    360     772    2102    2312     360 
   Size:        412    1330     210    2127

  Fragments arranged by size: 

                2127    1330     412     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    416    2287    2597    2743    3889     416 
   Size:       1871     310     146    1146     606

  Fragments arranged by size: 

                1871    1146     606     310     146
 
EcoICRI GAG'CTC

Cuts at:   1091    1091 
   Size:       4079
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    643    1048    1904    2336    3083    3351     643 
   Size:        405     856     432     747     268    1371

  Fragments arranged by size: 

                1371     856     747     432     405     268
 
EcoO109I rG'GnC_Cy

Cuts at:   1218    1218 
   Size:       4079
 
EcoRI G'AATT_C

Cuts at:   1083    1083 
   Size:       4079
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2098    2098 
   Size:       4079
 
FspI TGC'GCA

Cuts at:    605    1860     605 
   Size:       1255    2824
 
HaeII r_GCGC'y

Cuts at:    746    1513    1761    2783    3762     746 
   Size:        767     248    1022     979    1063

  Fragments arranged by size: 

                1063    1022     979     767     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    954     986    1921    1951    1953    1983     954 
   Size:         32     935      30       2      30    3050

  Fragments arranged by size: 

                3050     935      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1118    1118 
   Size:       4079
 
KasI G'GCGC_C

Cuts at:   1509    1757    1509 
   Size:        248    3831
 
KpnI G_GTAC'C

Cuts at:   1099    1099 
   Size:       4079
 
MluI A'CGCG_T

Cuts at:   3512    3512 
   Size:       4079
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    262     749    1069    2750    2934     262 
   Size:        487     320    1681     184    1407

  Fragments arranged by size: 

                1681    1407     487     320     184
 
MscI TGG'CCA

Cuts at:    454     532     719     968    1840     454 
   Size:         78     187     249     872    2693

  Fragments arranged by size: 

                2693     872     249     187      78
 
MslI CAynn'nnrTG

Cuts at:    484     518     616    2068    2195     484 
   Size:         34      98    1452     127    2368

  Fragments arranged by size: 

                2368    1452     127      98      34
 
MspA1I CmG'CkG

Cuts at:    173     439     585     651    1021    1190    1504    1864    2877 
   Size:        266     146      66     370     169     314     360    1013

Cuts at:   2877    3122     173 
   Size:        245    1130

  Fragments arranged by size: 

                1130    1013     370     360     314     266     245     169
                 146      66
 
NaeI GCC'GGC

Cuts at:    141     916    2260     141 
   Size:        775    1344    1960

  Fragments arranged by size: 

                1960    1344     775
 
NarI GG'CG_CC

Cuts at:   1510    1758    1510 
   Size:        248    3831
 
NcoI C'CATG_G

Cuts at:    479     479 
   Size:       4079
 
NgoMIV G'CCGG_C

Cuts at:    139     914    2258     139 
   Size:        775    1344    1960

  Fragments arranged by size: 

                1960    1344     775
 
NspI r_CATG'y

Cuts at:    903    1132    1632    2539     903 
   Size:        229     500     907    2443

  Fragments arranged by size: 

                2443     907     500     229
 
PciI A'CATG_T

Cuts at:   2535    2535 
   Size:       4079
 
PmeI GTTT'AAAC

Cuts at:   1078    1078 
   Size:       4079
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3218    3250    3218 
   Size:         32    4047
 
PstI C_TGCA'G

Cuts at:    435     596    1126    1811     435 
   Size:        161     530     685    2703

  Fragments arranged by size: 

                2703     685     530     161
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4079
 
PvuII CAG'CTG

Cuts at:    585    1504    1864     585 
   Size:        919     360    2800

  Fragments arranged by size: 

                2800     919     360
 
RsrII CG'GwC_CG

Cuts at:   2274    2274 
   Size:       4079
 
SacI G_AGCT'C

Cuts at:   1093    1093 
   Size:       4079
 
SalI G'TCGA_C

Cuts at:   1116    1116 
   Size:       4079
 
SapI GCTCTTCn'nnn_

Cuts at:   2102    2312    2102 
   Size:        210    3869
 
SbfI CC_TGCA'GG

Cuts at:   1126    1126 
   Size:       4079
 
ScaI AGT'ACT

Cuts at:   3596    3596 
   Size:       4079
 
SfcI C'TryA_G

Cuts at:     33     431     592    1122    1807    2800    2991      33 
   Size:        398     161     530     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     530     398     191     161
 
SfoI GGC'GCC

Cuts at:   1511    1759    1511 
   Size:        248    3831
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4079
 
SmaI CCC'GGG

Cuts at:   1101    1101 
   Size:       4079
 
SmlI C'TyrA_G

Cuts at:   1234    2149    2641    2903    1234 
   Size:        915     492     262    2410

  Fragments arranged by size: 

                2410     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4079
 
SphI G_CATG'C

Cuts at:   1132    1632    1132 
   Size:        500    3579
 
StuI AGG'CCT

Cuts at:    764     764 
   Size:       4079
 
StyI C'CwwG_G

Cuts at:    479    1213    1567     479 
   Size:        734     354    2991

  Fragments arranged by size: 

                2991     734     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1662    1795    2254    2463    1662 
   Size:        133     459     209    3278

  Fragments arranged by size: 

                3278     459     209     133
 
TatI w'GTAC_w

Cuts at:   3594    3594 
   Size:       4079
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3596    3628    3596 
   Size:         32    4047
 
Tth111I GACn'n_nGTC

Cuts at:   1876    1876 
   Size:       4079
 
XbaI T'CTAG_A

Cuts at:   1110    1110 
   Size:       4079
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1574    1574 
   Size:       4079
 
XmaI C'CCGG_G

Cuts at:   1099    1099 
   Size:       4079
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI
     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PciI     PmeI     PpiI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AloI     ApaI     AscI    AvrII
     BaeI     BbsI    BbvCI    BfrBI    BmgBI     BmtI   Bpu10I    BsaXI
    BseRI    BsiWI     BsmI    BsmBI    BsrGI   BstEII  BstZ17I   Bsu36I
     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SspI     SwaI     XhoI     XmnI     ZraI
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