Restriction map for pF1KB8184
 (Linear) (Six-base) MAPSORT of: KIBB8184.seq  Check: 8430  from: 1  to: 966



KIBB8184.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     580     667     966 
   Size:        580      87     299

  Fragments arranged by size: 

                 580     299      87
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     535     966 
   Size:        535     431
 
AleI CACnn'nnGTG

Cuts at:      0     410     966 
   Size:        410     556
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     509     966 
   Size:        509     457
 
AlwNI CAG_nnn'CTG

Cuts at:      0     331     966 
   Size:        331     635
 
ApoI r'AATT_y

Cuts at:      0     874     966 
   Size:        874      92
 
AvaI C'yCGr_G

Cuts at:      0     166     966 
   Size:        166     800
 
BanI G'GyrC_C

Cuts at:      0     592     966 
   Size:        592     374
 
BanII G_rGCy'C

Cuts at:      0     203     966 
   Size:        203     763
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     541     575     966 
   Size:        541      34     391

  Fragments arranged by size: 

                 541     391      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     145     319     807     966 
   Size:        145     174     488     159

  Fragments arranged by size: 

                 488     174     159     145
 
BlpI GC'TnA_GC

Cuts at:      0     433     966 
   Size:        433     533
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     765     966 
   Size:        765     201
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     940     966 
   Size:        940      26
 
BsaWI w'CCGG_w

Cuts at:      0     953     966 
   Size:        953      13
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     134     547     688     852     966 
   Size:        134     413     141     164     114

  Fragments arranged by size: 

                 413     164     141     134     114
 
Bsp1286I G_dGCh'C

Cuts at:      0     203     966 
   Size:        203     763
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     580     667     966 
   Size:        580      87     299

  Fragments arranged by size: 

                 580     299      87
 
BsrBI CCG'CTC

Cuts at:      0     185     224     234     447     966 
   Size:        185      39      10     213     519

  Fragments arranged by size: 

                 519     213     185      39      10
 
BsrFI r'CCGG_y

Cuts at:      0      31     806     966 
   Size:         31     775     160

  Fragments arranged by size: 

                 775     160      31
 
BssHII G'CGCG_C

Cuts at:      0     698     797     966 
   Size:        698      99     169

  Fragments arranged by size: 

                 698     169      99
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     331     821     966 
   Size:        331     490     145

  Fragments arranged by size: 

                 490     331     145
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     378     459     966 
   Size:        378      81     507

  Fragments arranged by size: 

                 507     378      81
 
BtgI C'CryG_G

Cuts at:      0     371     452     966 
   Size:        371      81     514

  Fragments arranged by size: 

                 514     371      81
 
BtsI GCAGTG_nn'

Cuts at:      0      78      87     122     267     314     835     966 
   Size:         78       9      35     145      47     521     131

  Fragments arranged by size: 

                 521     145     131      78      47      35       9
 
EaeI y'GGCC_r

Cuts at:      0     344     368     422     609     689     858     966 
   Size:        344      24      54     187      80     169     108

  Fragments arranged by size: 

                 344     187     169     108      80      54      24
 
EarI CTCTTCn'nnn_

Cuts at:      0     252     664     966 
   Size:        252     412     302

  Fragments arranged by size: 

                 412     302     252
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     308     966 
   Size:        308     658
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     535     940     966 
   Size:        535     405      26

  Fragments arranged by size: 

                 535     405      26
 
FspI TGC'GCA

Cuts at:      0     497     966 
   Size:        497     469
 
HaeII r_GCGC'y

Cuts at:      0     638     966 
   Size:        638     328
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     846     878     966 
   Size:        846      32      88

  Fragments arranged by size: 

                 846      88      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     641     961     966 
   Size:        154     487     320       5

  Fragments arranged by size: 

                 487     320     154       5
 
MscI TGG'CCA

Cuts at:      0     346     424     611     860     966 
   Size:        346      78     187     249     106

  Fragments arranged by size: 

                 346     249     187     106      78
 
MslI CAynn'nnrTG

Cuts at:      0     376     410     508     966 
   Size:        376      34      98     458

  Fragments arranged by size: 

                 458     376      98      34
 
MspA1I CmG'CkG

Cuts at:      0      65     331     477     543     913     966 
   Size:         65     266     146      66     370      53

  Fragments arranged by size: 

                 370     266     146      66      65      53
 
NaeI GCC'GGC

Cuts at:      0      33     808     966 
   Size:         33     775     158

  Fragments arranged by size: 

                 775     158      33
 
NcoI C'CATG_G

Cuts at:      0     371     966 
   Size:        371     595
 
NgoMIV G'CCGG_C

Cuts at:      0      31     806     966 
   Size:         31     775     160

  Fragments arranged by size: 

                 775     160      31
 
NspI r_CATG'y

Cuts at:      0     795     966 
   Size:        795     171
 
PstI C_TGCA'G

Cuts at:      0     327     488     966 
   Size:        327     161     478

  Fragments arranged by size: 

                 478     327     161
 
PvuII CAG'CTG

Cuts at:      0     477     966 
   Size:        477     489
 
SfcI C'TryA_G

Cuts at:      0     323     484     966 
   Size:        323     161     482

  Fragments arranged by size: 

                 482     323     161
 
StuI AGG'CCT

Cuts at:      0     656     966 
   Size:        656     310
 
StyI C'CwwG_G

Cuts at:      0     371     966 
   Size:        371     595
 

 Enzymes that do cut:

     AarI     AcuI     AleI     AlfI    AlwNI     ApoI     AvaI     BanI
    BanII     BcgI     BglI     BlpI     BplI     BpmI    BsaWI     BsgI
 Bsp1286I    BspMI    BsrBI    BsrFI   BssHII   BstAPI    BstXI     BtgI
     BtsI     EaeI     EarI     EciI  Eco57MI     FspI    HaeII    Hin4I
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI
     PstI    PvuII     SfcI     StuI     StyI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI
    BglII Bme1580I    BmgBI     BmrI     BmtI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI    BssSI
    BstBI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NarI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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